##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780666_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 132223 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.021720880633474 34.0 34.0 34.0 32.0 34.0 2 33.34400974111917 34.0 34.0 34.0 33.0 34.0 3 33.50305166272131 34.0 34.0 34.0 34.0 34.0 4 33.52505993662222 34.0 34.0 34.0 34.0 34.0 5 33.516959984269 34.0 34.0 34.0 34.0 34.0 6 37.255212784462614 38.0 38.0 38.0 36.0 38.0 7 36.85840587492343 38.0 37.0 38.0 35.0 38.0 8 36.91223917170235 38.0 37.0 38.0 35.0 38.0 9 36.70908994652973 38.0 37.0 38.0 34.0 38.0 10-11 36.41759754354386 38.0 37.0 38.0 34.0 38.0 12-13 36.296642792857526 38.0 37.0 38.0 34.0 38.0 14-15 36.551439613380424 38.0 37.0 38.0 34.0 38.0 16-17 36.68816696036242 38.0 37.0 38.0 34.0 38.0 18-19 36.70445383934717 38.0 37.0 38.0 34.0 38.0 20-21 36.434515931418886 38.0 37.0 38.0 34.0 38.0 22-23 36.50360754180438 38.0 37.0 38.0 34.0 38.0 24-25 35.83832994259698 38.0 36.5 38.0 32.5 38.0 26-27 36.079411297580606 38.0 37.0 38.0 33.0 38.0 28-29 35.95261414428655 38.0 37.0 38.0 33.0 38.0 30-31 35.675041407319455 38.0 36.5 38.0 31.5 38.0 32-33 36.18547832071576 38.0 37.0 38.0 34.0 38.0 34-35 36.246401911921524 38.0 37.0 38.0 33.5 38.0 36-37 36.38206666011209 38.0 37.0 38.0 34.0 38.0 38-39 36.032131323597255 38.0 36.5 38.0 32.5 38.0 40-41 35.50064285336137 38.0 36.0 38.0 31.0 38.0 42-43 35.90061865182305 38.0 36.5 38.0 32.5 38.0 44-45 36.09884815803605 38.0 37.0 38.0 33.5 38.0 46-47 36.406090468375396 38.0 37.0 38.0 34.0 38.0 48-49 36.222419700052185 38.0 37.0 38.0 34.0 38.0 50-51 36.37598602361163 38.0 37.0 38.0 34.0 38.0 52-53 36.29988353009688 38.0 37.0 38.0 34.0 38.0 54-55 36.47895600614114 38.0 37.0 38.0 34.0 38.0 56-57 36.53356828993443 38.0 37.0 38.0 34.0 38.0 58-59 36.589182668673374 38.0 37.0 38.0 34.0 38.0 60-61 36.27095891032573 38.0 37.0 38.0 33.5 38.0 62-63 36.527506560885776 38.0 37.0 38.0 34.0 38.0 64-65 36.52603556113536 38.0 37.0 38.0 34.0 38.0 66-67 35.93513609583809 38.0 37.0 38.0 32.5 38.0 68-69 35.430813852355485 38.0 36.0 38.0 31.0 38.0 70-71 35.45955696058931 38.0 36.0 38.0 31.0 38.0 72-73 35.20102024609939 38.0 36.0 38.0 29.0 38.0 74-75 35.82124516914607 38.0 36.5 38.0 32.0 38.0 76 35.24505570135302 38.0 36.0 38.0 30.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.01659386232420701 1101 2 0.39404767112309 1101 3 0.23560572847277683 1101 4 0.24514391701411853 1101 5 0.3388474185315289 1101 6 -1.3623880972521434 1101 7 0.17727721972239863 1101 8 0.5068602356188947 1101 9 0.5457325818111798 1101 10-11 0.33862631760329265 1101 12-13 0.5282257587030657 1101 14-15 0.6460054767677477 1101 16-17 -0.017549474390357034 1101 18-19 -0.17396641047556471 1101 20-21 -0.02810110108517705 1101 22-23 0.5172741169200279 1101 24-25 -0.8949921284602382 1101 26-27 0.06491467052562427 1101 28-29 0.8341492902428485 1101 30-31 1.058618937318002 1101 32-33 0.18617676159060892 1101 34-35 0.32388733254142466 1101 36-37 0.17836167962521898 1101 38-39 0.24974800427840904 1101 40-41 1.212404643831853 1101 42-43 0.7286953448862761 1101 44-45 0.472123657016958 1101 46-47 0.6455302031763708 1101 48-49 -1.4135204756773874 1101 50-51 -1.0634558540038768 1101 52-53 -1.57118874729305 1101 54-55 -0.851369834568203 1101 56-57 -1.0291724481224946 1101 58-59 -0.7824698711210587 1101 60-61 -1.4464338586275147 1101 62-63 -0.46524139643452145 1101 64-65 -1.1764259081397626 1101 66-67 -1.6048303742762968 1101 68-69 -1.1904465712194892 1101 70-71 -0.7424820203979934 1101 72-73 -1.3027039212054632 1101 74-75 -0.45274309185953143 1101 76 -0.7040882321157298 1102 1 -1.027043185868564 1102 2 0.03226303256887775 1102 3 0.18473976461735475 1102 4 0.2509421097851998 1102 5 0.08455524985683383 1102 6 0.20248389069966066 1102 7 0.03300011128865776 1102 8 0.5277563199562465 1102 9 0.6554087866304528 1102 10-11 0.3735472513382305 1102 12-13 0.9220510599078864 1102 14-15 0.1365551755629255 1102 16-17 -0.04239887198071557 1102 18-19 -0.034470928547854385 1102 20-21 0.5094177242160214 1102 22-23 0.4681777313778639 1102 24-25 -4.903218785086747 1102 26-27 -1.0426342752575053 1102 28-29 0.38830968180911185 1102 30-31 1.5236603529806558 1102 32-33 0.4022535688195248 1102 34-35 0.7050996819390107 1102 36-37 -2.4908966035073092 1102 38-39 -0.4951315137938792 1102 40-41 1.171986722145114 1102 42-43 0.7295613087416939 1102 44-45 0.9377298317157639 1102 46-47 1.1461890887185362 1102 48-49 -3.2383886985689543 1102 50-51 -0.3727179021966478 1102 52-53 -0.24142594608823487 1102 54-55 0.3642739907329968 1102 56-57 0.6826498410341344 1102 58-59 0.1890474481560389 1102 60-61 -5.831354792362454 1102 62-63 -2.8026284446272953 1102 64-65 -0.08100045633253217 1102 66-67 0.630109384759848 1102 68-69 -0.7380934085688793 1102 70-71 -1.2394134963016086 1102 72-73 -5.994947897109078 1102 74-75 -1.9445540858354349 1102 76 -0.35984877428440853 1103 1 0.40338821716292017 1103 2 0.41155439155319584 1103 3 0.03261513707492725 1103 4 0.3517971428205655 1103 5 0.1367640851981946 1103 6 0.058747655436029333 1103 7 0.6452879724105713 1103 8 0.5419744829307191 1103 9 0.36236564632730506 1103 10-11 0.3007398928721088 1103 12-13 -0.39180112301736614 1103 14-15 -0.5294313511892454 1103 16-17 0.10677848259889089 1103 18-19 0.31712901963195606 1103 20-21 -0.5062093000099139 1103 22-23 0.30141390186626893 1103 24-25 -2.966682316632287 1103 26-27 -0.699584657431366 1103 28-29 -0.42816589792919757 1103 30-31 0.6089045555975758 1103 32-33 0.9613447723433026 1103 34-35 0.41511717125110437 1103 36-37 -0.9547196375790818 1103 38-39 -0.5709509204527734 1103 40-41 -0.845340651867069 1103 42-43 -0.7271850314578145 1103 44-45 0.882120296801908 1103 46-47 0.7067701225312106 1103 48-49 -2.281917653096748 1103 50-51 -0.4906735959393629 1103 52-53 -0.056622215034984436 1103 54-55 -0.296514323815515 1103 56-57 0.17667432607105837 1103 58-59 -0.44414661843288883 1103 60-61 -4.077095820993108 1103 62-63 -1.1033798372947388 1103 64-65 -0.018848623193520098 1103 66-67 -0.43144327750210465 1103 68-69 -0.29227788842379 1103 70-71 0.04268263013963747 1103 72-73 -6.130862523356001 1103 74-75 -2.486933280031579 1103 76 -3.4570116192125084 1104 1 0.36079271056269135 1104 2 0.2823691480878594 1104 3 0.3350891295567848 1104 4 0.03631939126886152 1104 5 -0.3781410638532918 1104 6 0.1559808051868785 1104 7 0.595502151971715 1104 8 0.7462318480850172 1104 9 0.945283733572694 1104 10-11 -0.740959556380453 1104 12-13 0.4528531299767806 1104 14-15 0.6508073060360431 1104 16-17 0.23532992940368302 1104 18-19 0.45675005293907134 1104 20-21 -0.058169698646153734 1104 22-23 0.33644755865444864 1104 24-25 0.4902085829218734 1104 26-27 0.8522664659185821 1104 28-29 1.2099580639555896 1104 30-31 0.653084553849169 1104 32-33 0.46680853665836963 1104 34-35 0.2769276441945365 1104 36-37 1.0311182853162748 1104 38-39 1.3202764595629617 1104 40-41 1.4918719541299552 1104 42-43 0.1581414830976584 1104 44-45 0.6394635960413595 1104 46-47 0.8538000202495439 1104 48-49 0.03578931429550636 1104 50-51 0.13300417309639556 1104 52-53 0.36288560907551215 1104 54-55 -0.005142700421863822 1104 56-57 0.1693200993029862 1104 58-59 -0.4847860974083247 1104 60-61 0.3353200370364391 1104 62-63 0.4341234409638517 1104 64-65 0.7160495322396443 1104 66-67 -0.04622603823293758 1104 68-69 1.4815004138753665 1104 70-71 1.486045249249699 1104 72-73 1.268451227846029 1104 74-75 0.20979501789656751 1104 76 -0.05208111829459483 1105 1 0.7206497133880347 1105 2 0.2862684158039457 1105 3 0.1678530688983102 1105 4 0.22293647020560314 1105 5 -0.41554087934080997 1105 6 0.10350286019465926 1105 7 0.679404879296861 1105 8 -0.43363178565770255 1105 9 0.5269160924494756 1105 10-11 0.5219707324969107 1105 12-13 -0.4159231774671426 1105 14-15 0.2192102640017879 1105 16-17 0.18723775965219858 1105 18-19 0.733280929949963 1105 20-21 0.4079693776382243 1105 22-23 0.5600932658561959 1105 24-25 0.6132193077099686 1105 26-27 0.32831227690860487 1105 28-29 0.8738101944981693 1105 30-31 1.2230803734882159 1105 32-33 0.7480783573352952 1105 34-35 -0.5733866036287907 1105 36-37 0.659113009412458 1105 38-39 0.14230119576777156 1105 40-41 0.8273315055339836 1105 42-43 0.29166077041818994 1105 44-45 0.8250824336127138 1105 46-47 1.0502841925380721 1105 48-49 0.18578138602473615 1105 50-51 0.6542303162088885 1105 52-53 0.60544024206866 1105 54-55 0.9303788356445608 1105 56-57 0.5143115944307013 1105 58-59 0.3426298629214912 1105 60-61 0.7821898115852477 1105 62-63 -0.08685043898771738 1105 64-65 0.5922243578176847 1105 66-67 0.6637866470003004 1105 68-69 0.6739483755890276 1105 70-71 1.365463458325408 1105 72-73 1.0647162915604937 1105 74-75 0.28711728048089213 1105 76 0.9812176189480937 1106 1 -0.5442623515486247 1106 2 0.331204463030609 1106 3 0.13081887876179366 1106 4 0.30681299215862623 1106 5 0.22443267864714045 1106 6 0.23105482182300108 1106 7 -0.8428094854799184 1106 8 0.24683494660155247 1106 9 -0.4444588921772592 1106 10-11 0.5068434418807541 1106 12-13 1.1420263367377501 1106 14-15 -0.20697501456173484 1106 16-17 0.33830312676571594 1106 18-19 0.2829519421255924 1106 20-21 0.13829160122696038 1106 22-23 0.3000775851281219 1106 24-25 -0.17052500418277816 1106 26-27 1.0181028930689777 1106 28-29 0.5623555763700168 1106 30-31 0.8746684315376569 1106 32-33 0.3095329754634477 1106 34-35 1.0102131995934442 1106 36-37 1.0266185437870732 1106 38-39 0.564343380000949 1106 40-41 1.1842525484561364 1106 42-43 0.9214879749440712 1106 44-45 0.9432490182886397 1106 46-47 0.869202529765964 1106 48-49 0.9036128335711666 1106 50-51 0.21988952171867027 1106 52-53 0.5717574232271829 1106 54-55 0.04733861918901994 1106 56-57 0.24839258077923887 1106 58-59 0.5742588138500295 1106 60-61 0.9891062425285568 1106 62-63 0.696048343449867 1106 64-65 -0.83665892837098 1106 66-67 0.3655453482670552 1106 68-69 1.1933955530579752 1106 70-71 0.4946296125499856 1106 72-73 0.5266674660777753 1106 74-75 -0.09106498492312198 1106 76 0.10647896441606264 1107 1 0.5543750319900198 1107 2 -0.3524738584398932 1107 3 0.17878535572213394 1107 4 0.30894533089586673 1107 5 -0.002910293550733911 1107 6 0.14362893359618312 1107 7 0.7192438006992603 1107 8 -0.4497191345610574 1107 9 -0.4822317498083635 1107 10-11 0.3727884319991759 1107 12-13 -0.24828452407328427 1107 14-15 0.03471852304024026 1107 16-17 0.32065746648367366 1107 18-19 0.35411038901029457 1107 20-21 0.5515863989148215 1107 22-23 0.017981057794067112 1107 24-25 0.044391708300686616 1107 26-27 -0.632542752353551 1107 28-29 0.7239749650500542 1107 30-31 0.7000432625108104 1107 32-33 0.8638518901253178 1107 34-35 -0.09356928699585154 1107 36-37 0.926461776026251 1107 38-39 0.16240867265887005 1107 40-41 0.3476030692817318 1107 42-43 0.7615439952718788 1107 44-45 0.6359210541891969 1107 46-47 0.9241492584462989 1107 48-49 0.5265863687956198 1107 50-51 0.7819650971529413 1107 52-53 0.7145238169742996 1107 54-55 0.48131413072742646 1107 56-57 0.45956469840707115 1107 58-59 0.3569942998300988 1107 60-61 0.9926688084675988 1107 62-63 0.5326458143623896 1107 64-65 0.5903920545851804 1107 66-67 0.013742890505191951 1107 68-69 0.8543276897059613 1107 70-71 -0.3482459985470996 1107 72-73 0.7561167728819385 1107 74-75 -0.8293296921166018 1107 76 0.8907784131284799 1108 1 -0.4426978043424583 1108 2 0.2933393377897602 1108 3 0.014980728472778537 1108 4 0.3928939170141206 1108 5 0.22476408519819557 1108 6 -0.218263097252148 1108 7 -0.18838944694427084 1108 8 -0.7816814310477724 1108 9 -0.5978924181888203 1108 10-11 -0.04489451573004288 1108 12-13 0.7383924253697316 1108 14-15 0.18521381010107518 1108 16-17 -0.4703828077236878 1108 18-19 -0.12292474380890184 1108 20-21 0.5982530655814884 1108 22-23 -0.5077258830799707 1108 24-25 0.5210703715397642 1108 26-27 -1.1799394961410385 1108 28-29 -1.0574132097571507 1108 30-31 -0.08923522934865957 1108 32-33 0.323968428257281 1108 34-35 0.3137623325414225 1108 36-37 0.8098825129585592 1108 38-39 0.8000813376117435 1108 40-41 0.6695088104985203 1108 42-43 -0.9743463217803878 1108 44-45 0.11956115701696746 1108 46-47 0.5274260365097021 1108 48-49 0.7107920243226076 1108 50-51 0.47029414599612807 1108 52-53 -0.3400845806263817 1108 54-55 -0.6392240012348651 1108 56-57 -0.1454224481224955 1108 58-59 -0.0014907044543974735 1108 60-61 0.7557536413724861 1108 62-63 0.6677169368988061 1108 64-65 0.4220115918602403 1108 66-67 0.47883629239036907 1108 68-69 1.1289909287805173 1108 70-71 -0.7375861870646645 1108 72-73 -0.4717872545388033 1108 74-75 -1.1525139251928636 1108 76 0.4058701012176016 1109 1 -1.254005297882511 1109 2 0.21090006130396688 1109 3 0.21591096103091445 1109 4 0.199488232259597 1109 5 -0.23016097940128333 1109 6 -0.5210848026785015 1109 7 -0.4687822118021501 1109 8 0.16508859479202442 1109 9 0.6789447911135014 1109 10-11 0.2514775772932154 1109 12-13 0.34492472511133343 1109 14-15 0.4304127765093497 1109 16-17 0.6890073731548583 1109 18-19 0.7225532923668112 1109 20-21 0.7608887244962119 1109 22-23 0.3747030574885031 1109 24-25 -0.5957875095971872 1109 26-27 0.14753337207601191 1109 28-29 -1.3592736748734282 1109 30-31 0.10370533912679036 1109 32-33 0.5011880665001769 1109 34-35 -0.04364335221310256 1109 36-37 0.8040117119249643 1109 38-39 1.0458074358029563 1109 40-41 0.5651057097233334 1109 42-43 0.9073694404935111 1109 44-45 0.35421748776632 1109 46-47 0.5096896024011741 1109 48-49 0.6698411199298491 1109 50-51 -0.3309461640813893 1109 52-53 -0.28915951602689915 1109 54-55 -0.41816973766897547 1109 56-57 0.0024328490351308574 1109 58-59 -0.16125297835620955 1109 60-61 0.8624203080391553 1109 62-63 0.2255774020150909 1109 64-65 0.7114172766147675 1109 66-67 0.20621613735161048 1109 68-69 -0.5626163063616048 1109 70-71 -0.028387220656398426 1109 72-73 -0.3317252390349239 1109 74-75 -0.4364519096889907 1109 76 0.05695020457677202 1110 1 -0.33909963082647465 1110 2 -0.12149171243855506 1110 3 0.012788947650854254 1110 4 -0.3165855350406801 1110 5 0.3221522130520782 1110 6 0.031490327405386154 1110 7 0.3132361238319845 1110 8 0.6438893452079384 1110 9 0.7712034722221333 1110 10-11 -0.8939721413008144 1110 12-13 0.2595613751414234 1110 14-15 0.7308941753978786 1110 16-17 0.3949505256096444 1110 18-19 -0.32794757485912385 1110 20-21 0.7529562619285173 1110 22-23 -0.8926573899292833 1110 24-25 -1.2213405873643524 1110 26-27 0.9638230609365834 1110 28-29 0.2908059683250386 1110 30-31 0.008554724989238593 1110 32-33 0.6440962821385625 1110 34-35 0.8320637024044331 1110 36-37 0.9808231522279627 1110 38-39 0.9555699220866245 1110 40-41 -0.9288838835654047 1110 42-43 0.1265121257081887 1110 44-45 0.8174789652361412 1110 46-47 0.3823666757791102 1110 48-49 1.2067920243226098 1110 50-51 -0.19710311427784433 1110 52-53 0.7101254650357163 1110 54-55 -0.33705048525313686 1110 56-57 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38-39 -3.60982172865927 2116 40-41 -1.3140836721918774 2116 42-43 -1.3369852930959212 2116 44-45 -0.21126376879906417 2116 46-47 -0.8526847448948445 2116 48-49 0.2563469204650417 2116 50-51 -1.090774103262035 2116 52-53 -0.0838788437322151 2116 54-55 -0.5887353761112308 2116 56-57 -0.34655004456165983 2116 58-59 -2.494946688628481 2116 60-61 -1.5223472488352314 2116 62-63 0.043855413654497966 2116 64-65 -1.9607777256471763 2116 66-67 -0.6905128668578939 2116 68-69 -1.3453473501512363 2116 70-71 0.9645700938453245 2116 72-73 0.05621670193993822 2116 74-75 -0.05741204586546189 2116 76 -1.6759261401276007 2117 1 0.6806472248973066 2117 2 0.46508202784823993 2117 3 0.3750976875370995 2117 4 -0.2304043285999171 2117 5 0.23617928987656 2117 6 0.29205269222153873 2117 7 0.6549087986697657 2117 8 0.4826460543323421 2117 9 -1.0236935877794693 2117 10-11 -0.35239159175343104 2117 12-13 -0.8983093290162358 2117 14-15 0.05559977501336277 2117 16-17 -0.6801956732207657 2117 18-19 -1.1428077847445692 2117 20-21 -0.15747792857055742 2117 22-23 0.2232682689668124 2117 24-25 -1.1291752424953287 2117 26-27 -1.5685008996498127 2117 28-29 -1.265366426131422 2117 30-31 -0.20973815332526158 2117 32-33 -1.4329204606316068 2117 34-35 0.36125940856481265 2117 36-37 -1.4890999431817917 2117 38-39 0.08293513878133751 2117 40-41 -0.1353566865774951 2117 42-43 0.4887647893307161 2117 44-45 -0.24186574356783552 2117 46-47 -0.781416068753451 2117 48-49 -0.7063658704142313 2117 50-51 -0.3492380177465648 2117 52-53 0.5310499222975906 2117 54-55 -0.3632941766734632 2117 56-57 -0.024018939350561652 2117 58-59 -0.8753152658579069 2117 60-61 -0.20775513055733796 2117 62-63 -0.13294972976785857 2117 64-65 -0.9317895777303988 2117 66-67 -0.6143332982529088 2117 68-69 0.2815289404764201 2117 70-71 -0.11226454963775723 2117 72-73 0.9801542659290376 2117 74-75 0.7102229169124001 2117 76 0.7687005105743268 2118 1 -0.03241102594395073 2118 2 0.3609296543633178 2118 3 -0.552505305046779 2118 4 0.21235760416495708 2118 5 0.3639521671348831 2118 6 0.3076363440886354 2118 7 0.7447353202810518 2118 8 0.5263260177418019 2118 9 -1.424082362322899 2118 10-11 -0.6870807354693298 2118 12-13 -0.9672351351516895 2118 14-15 0.6117352253710919 2118 16-17 -2.2259991950607443 2118 18-19 -0.5485299579615983 2118 20-21 -0.5000895787388089 2118 22-23 0.6864081951323158 2118 24-25 0.6132044497520468 2118 26-27 -1.9613510417648783 2118 28-29 -0.23864226003647815 2118 30-31 -0.6194624174305972 2118 32-33 0.19572634259619548 2118 34-35 -0.09082984622952495 2118 36-37 -2.33475808294461 2118 38-39 0.09066979198790648 2118 40-41 -0.06457685058155249 2118 42-43 0.6853817974002396 2118 44-45 -1.8055561614187923 2118 46-47 0.21008152999201712 2118 48-49 0.5595853204119976 2118 50-51 -0.3515494294228674 2118 52-53 -1.2473322528796373 2118 54-55 -0.5425796809369174 2118 56-57 0.37826470271549084 2118 58-59 -0.5395316727970396 2118 60-61 -0.8810620010856169 2118 62-63 -1.2465437707361957 2118 64-65 0.3801121505194587 2118 66-67 0.3441919720924176 2118 68-69 -1.2086291829513343 2118 70-71 -0.11801449246503637 2118 72-73 1.1976801570068218 2118 74-75 0.25775795562650217 2118 76 0.6527378852027041 2119 1 0.646776315740361 2119 2 -0.8466937885042398 2119 3 0.022931039031782063 2119 4 -0.41172720099830684 2119 5 -0.16344295413927767 2119 6 -0.7560022276869276 2119 7 -0.6616979355571075 2119 8 0.08809082568100735 2119 9 -0.13883651756770377 2119 10-11 -0.08679099606130336 2119 12-13 -0.19576492452674898 2119 14-15 -0.1672126909341216 2119 16-17 -0.2997699712847677 2119 18-19 -0.15387712476128002 2119 20-21 -1.2154943464267944 2119 22-23 -0.09623519985015605 2119 24-25 0.780871613775787 2119 26-27 0.4335967357430164 2119 28-29 0.04687250452855807 2119 30-31 0.9377047292434781 2119 32-33 0.14023343860925053 2119 34-35 0.442967301485524 2119 36-37 0.11433799950099655 2119 38-39 -0.9072395733613376 2119 40-41 -1.2686609617582079 2119 42-43 0.20115264302292246 2119 44-45 0.335002150805785 2119 46-47 0.13762065348692687 2119 48-49 -0.6109098390314287 2119 50-51 0.5309152640085486 2119 52-53 -0.06654006716882321 2119 54-55 0.18516937350632645 2119 56-57 -1.0590249325945464 2119 58-59 -0.3202319052825544 2119 60-61 0.2966853183911198 2119 62-63 -0.35706981258359605 2119 64-65 -0.12146666900932246 2119 66-67 0.05433111640693511 2119 68-69 0.20224558716560637 2119 70-71 -0.10893194275824669 2119 72-73 0.5532355197883234 2119 74-75 -0.3884476726048689 2119 76 0.12473552564824075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 4.0 23 25.0 24 43.0 25 98.0 26 170.0 27 302.0 28 598.0 29 1049.0 30 1699.0 31 2911.0 32 4260.0 33 6946.0 34 10748.0 35 17034.0 36 30604.0 37 55731.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.48876519213753 22.238944812929674 11.929089492750883 27.34320050218192 2 18.70173872926798 19.089719640304637 36.973143855456314 25.23539777497107 3 18.080818011994886 23.96708590789802 29.92217692837101 28.02991915173608 4 13.865968855645386 18.281237001126886 38.2142289919303 29.63856515129743 5 12.941772611421614 36.80448938535656 37.09263895086332 13.161099052358516 6 29.40789423927758 39.006829371591934 17.1392269121106 14.446049477019884 7 28.693948859124358 29.187811500268484 22.56339668590185 19.554842954705308 8 24.48817527964121 35.3584474713174 20.042655211271867 20.110722037769527 9 23.55565975662328 15.97301528478404 22.259364860878968 38.21196009771371 10-11 24.96426491608873 25.632076113837986 27.869583960430482 21.5340750096428 12-13 24.193597180520786 23.796918841653873 26.92988360572669 25.079600372098653 14-15 21.88159397381696 25.084894458604023 23.9315398985048 29.101971669074217 16-17 22.603858632764346 27.90210477753492 26.670851515999484 22.823185073701246 18-19 22.446170484711434 27.60298888998132 27.118579974739642 22.8322606505676 20-21 21.267101790157536 27.509586078065084 27.252822882554472 23.970489249222904 22-23 22.08352555909335 27.78223153309182 27.729668817074188 22.404574090740642 24-25 22.554321109035495 28.232607035084666 26.941984374881827 22.271087480998013 26-27 21.763611474554352 28.205002155449506 27.748198119842993 22.28318825015315 28-29 21.602141836140458 26.78391807779282 29.395037172050248 22.21890291401647 30-31 21.609326667826323 26.940471778737436 29.170794793644074 22.27940675979217 32-33 21.439537750618275 27.626812279255503 27.51147682324558 23.42217314688065 34-35 21.983316064527354 26.96391701897552 28.70340258502681 22.34936433147032 36-37 22.042685463194754 28.165674655695305 27.36967093470879 22.421968946401154 38-39 21.48794082723883 27.62378708696672 28.056011435226853 22.8322606505676 40-41 21.62180558601756 25.64266428684873 29.560666449861216 23.174863677272487 42-43 21.698947989381576 25.526194383730516 29.497893709868933 23.276963917018975 44-45 20.758113187569485 25.54018589806615 30.214107984238748 23.48759293012562 46-47 20.89689388381749 24.421242900251848 30.431921829031257 24.249941386899405 48-49 19.919000476467787 24.737375494429862 31.20863994917677 24.13498407992558 50-51 18.854132790815516 25.761024935147443 31.17574098303623 24.20910129100081 52-53 18.393169115811926 25.712621858526884 31.956996891614924 23.93721213404627 54-55 17.477292150382308 26.445096541448915 31.03885103196872 25.038760276200055 56-57 18.18783418921065 26.184930004613417 29.58864947853248 26.038586327643447 58-59 17.603972077475174 25.668000272267307 30.668264976592575 26.05976267366494 60-61 18.440815894360284 26.17850147099975 29.43625541698494 25.94442721765502 62-63 17.499224794476 27.730046966110283 28.029162853663887 26.741565385749833 64-65 18.457076302912505 28.316177972062352 26.225770100512015 27.000975624513135 66-67 18.0585072188651 27.723618432496615 25.462287196629934 28.75558715200835 68-69 18.063045007298275 28.166430953767495 25.292876428457983 28.477647610476243 70-71 18.582621782897075 27.506560885776306 25.674806954917074 28.23601037640955 72-73 18.77169630094613 26.951816249820382 25.53905145095785 28.73743599827564 74-75 19.158542764874493 27.02139567246243 26.018166279694157 27.801895282968925 76 19.04887954440604 26.3093410374897 27.15110079184408 27.490678626260184 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 6.5 17 12.0 18 12.5 19 17.5 20 23.5 21 25.0 22 38.0 23 60.5 24 99.0 25 128.0 26 211.5 27 364.5 28 531.0 29 628.0 30 822.0 31 1132.5 32 1601.5 33 1954.0 34 2236.5 35 2878.0 36 3599.5 37 3962.0 38 4665.5 39 6140.0 40 7675.0 41 8785.5 42 9132.0 43 9303.5 44 9866.5 45 10324.0 46 10390.0 47 10470.5 48 9931.0 49 8886.0 50 8461.0 51 7814.5 52 6375.0 53 4960.0 54 4338.0 55 3975.0 56 3117.0 57 2234.0 58 1846.0 59 1478.5 60 979.0 61 654.0 62 461.0 63 379.5 64 227.0 65 135.5 66 88.0 67 61.0 68 46.5 69 24.5 70 17.5 71 18.0 72 16.0 73 8.5 74 2.0 75 1.0 76 2.0 77 2.5 78 1.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 132223.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.63373996959682 #Duplication Level Percentage of deduplicated Percentage of total 1 57.897529501655484 20.63105511144052 2 14.31997622888191 10.205486186215712 3 8.44511418626369 9.027930087806206 4 5.446132948467612 7.76264341302194 5 3.3067323202309193 5.891561982408507 6 2.2306647423380594 4.769215643269326 7 1.470837931912726 3.6688019482238343 8 1.0994142117327446 3.134099211181111 9 0.8914169284319552 2.8588067129016888 >10 4.811528992274386 28.749914916466878 >50 0.07216232277782494 1.6653683549760632 >100 0.0042448425163426435 0.22008273900909828 >500 0.0021224212581713218 0.39251869946983503 >1k 0.0021224212581713218 1.0225149936092814 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 1352 1.0225149936092814 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 519 0.39251869946983503 RNA PCR Primer, Index 48 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 190 0.14369663371728067 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006806682649765926 0.0 2 0.0 0.0 0.0 0.009831874938550781 0.0 3 0.0 0.0 0.0 0.012100769155139423 0.0 4 0.0 0.0 0.0 0.014369663371728066 0.0 5 0.0 0.0 0.0 0.014369663371728066 0.0 6 0.0 0.0 0.0 0.014369663371728066 0.0 7 0.0 0.0 0.0 0.01512596144392428 0.0 8 0.0 0.0 0.0 0.017394855660512923 0.0 9 0.0 0.0 0.0 0.021932644093690206 0.0 10 0.0 0.0 0.0 0.031764519032240986 0.0 11 0.0 0.0 0.0 0.03403341324882963 0.0 12 0.0 0.0 0.0 0.035546009393222056 0.0 13 0.0 0.0 0.0 0.038571201682006916 0.0 14 0.0 0.0 0.0 0.038571201682006916 0.0 15 0.0 0.0 0.0 0.042352692042987986 0.0 16 0.0 0.0 0.0 0.04462158625957662 0.0 17 0.0 0.0 0.0 0.04764677854836148 0.0 18 0.0 0.0 0.0 0.05520975927032362 0.0 19 0.0 0.0 0.0 0.06050384577569712 0.0 20 0.0 0.0 0.0 0.061260143847893335 0.0 21 0.0 0.0 0.0 0.0703357207142479 0.0 22 0.0 0.0 0.0 0.07487350914742519 0.0 23 0.0 0.0 0.0 0.08016759565279868 0.0 24 0.0 0.0 0.0 0.08243648986938733 0.0 25 0.0 0.0 0.0 0.08394908601377975 0.0 26 0.0 0.0 0.0 0.09075576866354568 0.0 27 0.0 0.0 0.0 0.12630177805676773 0.0 28 0.0 0.0 0.0 0.1678981720275595 0.0 29 0.0 0.0 0.0 0.20117528720419292 0.0 30 0.0 0.0 0.0 0.24050278695839605 0.0 31 0.0 0.0 0.0 0.2987377385175045 0.0 32 0.0 0.0 0.0 0.37436754573712594 0.0 33 0.0 0.0 0.0 0.4447032664513738 0.0 34 0.0 0.0 0.0 0.5173078813822104 0.0 35 0.0 7.562980721962139E-4 0.0 0.5891561982408506 0.0 36 0.0 7.562980721962139E-4 0.0 0.827390090982658 0.0 37 0.0 7.562980721962139E-4 0.0 1.3000763861052917 0.0 38 0.0 7.562980721962139E-4 0.0 1.9330978725335228 0.0 39 0.0 7.562980721962139E-4 0.0 2.707547098462446 0.0 40 0.0 7.562980721962139E-4 0.0 3.721742813277569 0.0 41 0.0 7.562980721962139E-4 0.0 5.0354325646823925 0.0 42 0.0 7.562980721962139E-4 0.0 6.678111977492569 0.0 43 0.0 7.562980721962139E-4 0.0 8.282976486692936 0.0 44 0.0 7.562980721962139E-4 0.0 9.635993737851962 0.0 45 0.0 7.562980721962139E-4 0.0 10.86497810517081 0.0 46 0.0 7.562980721962139E-4 0.0 12.19152492380297 0.0 47 0.0 7.562980721962139E-4 0.0 13.402358137389108 0.0 48 0.0 7.562980721962139E-4 0.0 14.477813996052124 0.0 49 0.0 7.562980721962139E-4 0.0 15.514698653033133 0.0 50 0.0 7.562980721962139E-4 0.0 16.449483070267654 0.0 51 0.0 7.562980721962139E-4 0.0 17.537795996158007 0.0 52 0.0 7.562980721962139E-4 0.0 18.98535050634156 0.0 53 0.0 7.562980721962139E-4 0.0 20.374670064966004 0.0 54 0.0 7.562980721962139E-4 0.0 21.818443084788576 0.0 55 0.0 7.562980721962139E-4 0.0 22.913562693328696 0.0 56 0.0 7.562980721962139E-4 0.0 23.85515379321298 0.0 57 0.0 7.562980721962139E-4 0.0 24.769518162498205 0.0 58 0.0 7.562980721962139E-4 0.0 25.730016714187396 0.0 59 0.0 7.562980721962139E-4 0.0 26.66404483334972 0.0 60 0.0 7.562980721962139E-4 0.0 27.469502280238686 0.0 61 0.0 7.562980721962139E-4 0.0 28.27042193869448 0.0 62 0.0 7.562980721962139E-4 0.0 29.078148279800036 0.0 63 0.0 7.562980721962139E-4 0.0 30.096881783048335 0.0 64 0.0 7.562980721962139E-4 0.0 31.09292634413075 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATATCG 15 0.00222054 70.0 36 AGGCCAG 15 0.00222054 70.0 27 CCGCCAG 15 0.00222054 70.0 22 CCGTGCG 45 2.6791895E-8 54.444443 9 AAATACG 20 0.006938503 52.5 4 CGTGCGA 40 7.4336094E-7 52.5 10 GGGTCCC 20 0.006938503 52.5 8 TCGGGCC 20 0.006938503 52.5 43 ACGGGCC 20 0.006938503 52.5 8 ATATCGT 20 0.006938503 52.5 37 GGGGTCC 20 0.006938503 52.5 7 CCCTTGA 20 0.006938503 52.5 12 CGTCCCA 20 0.006938503 52.5 31 CCACCGG 20 0.006938503 52.5 53 TTGAGTG 45 1.6744125E-6 46.666664 43 GTAATGG 45 1.6744125E-6 46.666664 58 CCCGTGC 45 1.6744125E-6 46.666664 8 ATTTGGC 30 5.86044E-4 46.666664 40 GATGTAT 40 4.5726672E-5 43.75 24 TATGCCG 340 0.0 42.205883 43 >>END_MODULE