FastQCFastQC Report
Wed 25 May 2016
SRR1780665_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780665_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066347
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGT207.8768027E-443.9766329
ATCGTTC207.887732E-443.96425231
CGCTATA250.002334469535.25575614
ACTTACG250.00235013135.2077436
TTACGAT250.00235013135.2077438
GCAATCG250.002358807535.18130528
CGTGCGA1500.029.34943410
ACCCGCA300.00578081129.2957556
CGAGAAT1750.026.44181814
ATTTGCT508.328047E-526.40580638
TAATTGC551.5910702E-424.00527840
TCGACTC551.5991973E-423.98725330
CGACTCA551.602254E-423.98050131
GCGAACT852.3641951E-723.28174428
TTGTACG852.378165E-723.2675519
GCGAAGT952.7119313E-823.13582432
CCGTGCG2100.023.0213349
GATGTAT1900.022.0131124
GCATATA500.002576945822.0048391
TTTCGAC602.8828063E-421.98831628
Produced by FastQC (version 0.11.5)