FastQCFastQC Report
Wed 25 May 2016
SRR1780665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066347
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT153321.4378058924533947No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT52860.4957110584078166Illumina PCR Primer Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC23300.21850298261260173No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG11240.10540658903715207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCC207.845673E-444.0120113
GAACGAT207.856554E-443.9996266
CCGTCTT6350.037.7634644
GCGATGC250.002349521335.20960612
GACCCGT250.0023538535.196438
GATATCC250.0023538535.19648
ATACGTC250.0023538535.196436
GCCGTCT6850.035.00700443
AGTGCCG300.005737057429.3413414
TATTGCG1050.029.3385879
ATCGTAA300.005743636729.3344572
ACGATTG300.00574758729.3303328
CGCGTAG300.00574758729.33033219
ATGCCGT23600.027.96586244
CGAGAAT1350.027.71126414
TGCCGTC8750.027.4029142
TATGCCG24150.027.32895943
CATCGTT1300.027.07415232
TTAGACC508.3463296E-526.39729939
CGTATGC26450.025.03335641
Produced by FastQC (version 0.11.5)