FastQCFastQC Report
Wed 25 May 2016
SRR1780664_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780664_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45602
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC150.002145467670.533711
GGGGACA150.002212152169.99232568
CGTGCAC150.002212152169.9923253
GCGTGCA150.002212152169.9923252
GACGAAA150.002212152169.99232518
GTGCACA200.006912512752.494244
AAATACC200.006912512752.4942423
AGGACCA200.006912512752.4942442
TTATCAA200.006912512752.4942432
TGCATCT200.006912512752.4942444
AGACGAA200.006912512752.4942417
GGGACAG200.006912512752.4942469
CCCCCAG200.006912512752.4942412
CCCCAGA200.006912512752.4942413
TTAGTCG200.006912512752.494243
TTGCATC200.006912512752.4942443
GAATTGC200.006912512752.4942440
GGACAGC200.006912512752.4942470
CATTTAC200.006912512752.4942414
CTAATGT200.006912512752.4942461
Produced by FastQC (version 0.11.5)