FastQCFastQC Report
Wed 25 May 2016
SRR1780664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45602
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT9302.0393842375334414No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT4130.9056620323670014TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1270.2784965571685452No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1180.2587605806762861No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG840.18420244726108503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCA207.847379E-570.0000115
CGCCATT207.847379E-570.0000117
TTGGCGT252.7974547E-670.022
TGAAGGT252.7974547E-670.028
ATTGGCG252.7974547E-670.021
GTGACGA150.002211190870.070
GTGAAGG252.7974547E-670.027
CATTGGC308.2522965E-658.33333220
AGCCATA308.2522965E-658.33333247
TGGCGTG308.2522965E-658.33333223
AAGGTAG308.2522965E-658.33333230
AGCGGAT308.2522965E-658.33333235
CGGATGA308.2522965E-658.33333237
TTACGTC308.2522965E-658.33333256
GCGTGAA308.2522965E-658.33333225
CGAGTGA252.3666851E-455.99999664
GTCTCGA252.3666851E-455.99999660
AGGCGCC252.3666851E-455.99999614
TCTCGAG252.3666851E-455.99999661
CTCGAGT252.3666851E-455.99999662
Produced by FastQC (version 0.11.5)