FastQCFastQC Report
Wed 25 May 2016
SRR1780663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399876
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG207.89951E-443.94421444
TATGATC353.2309187E-431.40050742
TGCGTGT353.2309187E-431.40050733
CGACGTT502.3244957E-630.8535714
CATCGTA300.00571288329.36225124
GTACAAG901.6370905E-1129.3438571
ATCGTAC300.005747975329.32548725
TTTGACC300.005765584829.30714228
TACGAAT300.005779703629.2924827
ATACGAA300.005783237529.288826
GCTAAAC300.005783237529.288823
AATTATC852.6557245E-1028.44872530
TTATCAA852.6557245E-1028.44516632
TATACCG703.6312485E-828.2427886
ACGTTAC554.878875E-628.05221716
CCGACGT554.902746E-628.03463213
TACGGAC554.931524E-628.01355638
GATGCAT554.931524E-628.01355635
ATTCGCC554.999245E-627.964543
CAAGGAA953.274181E-1127.74734
Produced by FastQC (version 0.11.5)