SRR1780663_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780663_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399876
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT	13037	3.2602606808110517	No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT	4722	1.1808660684812293	RNA PCR Primer, Index 34 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC	2089	0.5224119477037882	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG	1201	0.3003431063629725	No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC	993	0.24832698136422293	No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC	522	0.13054046754493895	RNA PCR Primer, Index 34 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT	474	0.11853674639138133	RNA PCR Primer, Index 34 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	20	7.8469084E-4	44.00388	10
CGTCCAG	20	7.851744E-4	43.998375	27
ATACCGA	20	7.851744E-4	43.998375	6
ATTGGCG	130	0.0	40.613888	21
TGAAGGT	130	0.0	38.921642	28
ATCACGT	40	4.116755E-7	38.498577	24
CACGTAG	40	4.116755E-7	38.498577	26
GAGGCGC	150	0.0	38.136692	13
CGCCATT	155	0.0	36.901863	17
GACAGTA	30	1.2998599E-4	36.665314	43
AATCGTT	30	1.2998599E-4	36.665314	39
CCGTCTT	625	0.0	36.606647	44
GCGTGAA	145	0.0	36.41245	25
GAGCAAC	25	0.0023499	35.203102	11
CGCACGG	25	0.0023499	35.203102	11
TAGGTCC	25	0.0023513425	35.1987	34
ACCCGAT	25	0.0023513425	35.1987	27
AGGCGCC	160	0.0	34.37803	14
TTGAGGC	160	0.0	34.37803	11
CTCCGGT	45	1.0352087E-6	34.22096	7