##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780662_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3713 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.084029086991652 23.0 12.0 32.0 12.0 34.0 2 21.038782655534607 21.0 11.0 32.0 11.0 34.0 3 20.916509561001885 21.0 11.0 31.0 11.0 34.0 4 21.829248586049015 23.0 11.0 32.0 11.0 34.0 5 21.771882574737408 23.0 11.0 32.0 11.0 34.0 6 23.387018583355776 26.0 11.0 34.0 11.0 37.0 7 22.486937786156748 23.0 11.0 34.0 11.0 37.0 8 22.03151090762187 21.0 11.0 34.0 11.0 37.0 9 23.043630487476435 23.0 12.0 34.0 12.0 37.0 10-11 21.737139779154322 21.0 11.0 33.5 11.0 37.0 12-13 21.858470239698356 21.0 11.0 33.0 11.0 37.0 14-15 21.675868569889577 21.0 11.0 33.0 11.0 37.0 16-17 21.305548074333423 21.0 11.0 32.0 11.0 37.0 18-19 21.67452194990574 21.0 11.0 32.0 11.0 37.0 20-21 21.567600323188795 21.5 11.0 32.0 11.0 36.5 22-23 21.147589550228926 21.0 11.0 31.0 11.0 37.0 24-25 21.144896310261245 21.0 11.0 31.0 11.0 37.0 26-27 21.06733099919203 21.0 11.0 31.0 11.0 36.5 28-29 20.43926743872879 21.0 11.0 31.0 11.0 36.5 30-31 20.153784002154595 16.0 11.0 27.5 11.0 36.0 32-33 20.474952868300562 21.0 11.0 29.0 11.0 36.0 34-35 20.888903851333154 21.0 11.0 31.0 11.0 36.0 36-37 20.39846485321842 16.0 11.0 27.0 11.0 36.0 38-39 19.966334500403985 11.0 11.0 27.0 11.0 36.0 40-41 20.4282251548613 21.0 11.0 27.0 11.0 36.0 42-43 20.55346081335847 21.0 11.0 29.0 11.0 36.0 44-45 20.216536493401563 20.5 11.0 27.0 11.0 36.0 46-47 20.367088607594937 21.0 11.0 27.0 11.0 36.0 48-49 20.652437382170753 21.0 11.0 29.0 11.0 36.0 50-51 20.718287099380554 21.0 11.0 31.0 11.0 36.0 52-53 20.801642876380285 21.0 11.0 29.5 11.0 36.0 54-55 20.926205224885535 21.0 11.0 31.0 11.0 36.0 56-57 20.09399407487207 20.0 11.0 27.0 11.0 36.0 58-59 20.346754645838942 16.0 11.0 29.0 11.0 36.0 60-61 20.54847831941826 21.0 11.0 29.5 11.0 35.5 62-63 19.946000538647993 17.5 11.0 27.0 11.0 35.5 64-65 20.20576353353084 16.0 11.0 28.0 11.0 35.0 66-67 20.637759224346887 20.5 11.0 29.0 11.0 36.0 68-69 19.853353083759764 19.0 11.0 27.0 11.0 35.0 70-71 20.2888499865338 19.5 11.0 28.0 11.0 34.0 72-73 19.776326420684082 19.0 11.0 27.0 11.0 34.0 74-75 19.96808510638298 19.0 11.0 27.0 11.0 34.0 76 19.709668731483976 19.0 11.0 26.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.9118990658464305 1101 2 1.8280196411775371 1101 3 -1.213651041940512 1101 4 -4.793414334203806 1101 5 -5.903778458383719 1101 6 -2.629944981918669 1101 7 -0.4557956663219791 1101 8 0.21086472007524648 1101 9 0.9131237183868741 1101 10-11 -2.624084942834937 1101 12-13 1.0648444756997435 1101 14-15 -6.784531842426578 1101 16-17 -3.4101417953391646 1101 18-19 0.6510698731093427 1101 20-21 -3.097359986504724 1101 22-23 -4.118568346857813 1101 24-25 -0.009396086369772405 1101 26-27 -1.552499145591252 1101 28-29 -0.9176908617698025 1101 30-31 -3.5841827397748496 1101 32-33 -1.2596614277535352 1101 34-35 -3.397304888094361 1101 36-37 -1.9944604737367904 1101 38-39 -3.0958627629680286 1101 40-41 -2.6292875983665454 1101 42-43 -3.0544141165851677 1101 44-45 -5.123164043558781 1101 46-47 -4.13345780681307 1101 48-49 -2.8647969355206158 1101 50-51 -5.865766105239796 1101 52-53 -2.0518176998440083 1101 54-55 -2.466379453879455 1101 56-57 3.2742797042139173 1101 58-59 -0.1820806386595848 1101 60-61 -3.097923348581247 1101 62-63 0.9742780976333592 1101 64-65 -4.147518526794837 1101 66-67 1.3221578859736738 1101 68-69 0.4633648880359438 1101 70-71 -2.0722771745798063 1101 72-73 0.16534587488535024 1101 74-75 0.23951750004381367 1101 76 -3.411050353155616 1102 1 1.6618990658464305 1102 2 3.089383277541174 1102 3 2.8658944126049413 1102 4 1.1270402112507405 1102 5 -1.3696875492928093 1102 6 2.074600472626784 1102 7 1.669204333678021 1102 8 -2.05049891628839 1102 9 2.9813055365686907 1102 10-11 -2.0274940337440306 1102 12-13 2.34893538479065 1102 14-15 -3.5799863878811244 1102 16-17 -2.2283236135209847 1102 18-19 -1.360293763254294 1102 20-21 1.6810491044043694 1102 22-23 1.09165892586946 1102 24-25 -1.4355324500061393 1102 26-27 -3.2343173274094354 1102 28-29 -0.9176908617698025 1102 30-31 0.08627180567969717 1102 32-33 -2.5948887004808086 1102 34-35 -2.840486706276181 1102 36-37 -1.9887786555549702 1102 38-39 3.693909964304698 1102 40-41 -1.5611057801847252 1102 42-43 -0.6737322984033476 1102 44-45 1.8825177746230395 1102 46-47 -2.3323214431767063 1102 48-49 -0.27388784461152227 1102 50-51 1.4751429856692937 1102 52-53 0.11295502742871832 1102 54-55 -0.4322885447885483 1102 56-57 -0.7029930230588093 1102 58-59 0.39746481588586846 1102 60-61 0.7941221059642061 1102 62-63 -1.7359491750939142 1102 64-65 0.6877087459324365 1102 66-67 -1.9448875685717795 1102 68-69 -0.24118056650950948 1102 70-71 -1.9359135382161696 1102 72-73 2.03466405670353 1102 74-75 -0.6979824999561863 1102 76 -5.43377762588289 1103 1 -0.9214342674869016 1103 2 -0.5469803588224629 1103 3 -2.088651041940512 1103 4 0.1649189991295259 1103 5 2.3878882082829485 1103 6 6.120055018081331 1103 7 0.1692043336780209 1103 8 -2.7474686132580857 1103 9 2.913123718386874 1103 10-11 3.813415057165063 1103 12-13 0.6481778090330756 1103 14-15 5.798801490906758 1103 16-17 1.8398582046608354 1103 18-19 -0.5989301268906573 1103 20-21 -0.5348599865047241 1103 22-23 3.652264986475519 1103 24-25 -1.0718960863697724 1103 26-27 -0.38583247892458417 1103 28-29 -2.0010241951031347 1103 30-31 -8.49406441513878E-4 1103 32-33 0.636171905579797 1103 34-35 2.186028445238975 1103 36-37 3.8180395262632096 1103 38-39 -1.075029429634693 1103 40-41 -2.0042875983665454 1103 42-43 -0.6169141165851677 1103 44-45 0.35600262310788366 1103 46-47 -0.15429114014640533 1103 48-49 2.0102030644793842 1103 50-51 3.0092338947602038 1103 52-53 -2.780984366510676 1103 54-55 2.783620546120545 1103 56-57 0.02427970421391734 1103 58-59 0.1929193613404152 1103 60-61 -1.2437566819145829 1103 62-63 -0.33822190236664085 1103 64-65 -1.460018526794837 1103 66-67 1.9679912193070095 1103 68-69 1.0883648880359438 1103 70-71 -1.9472771745798063 1103 72-73 -1.4804874584479855 1103 74-75 0.6353508333771494 1103 76 3.7556163135110516 1104 1 -1.3381009341535695 1104 2 -4.239288051130156 1104 3 -1.9732664265558952 1104 4 0.8123548965654237 1104 5 0.49045231084705065 1104 6 -5.918406520380209 1104 7 -0.10002643555274915 1104 8 -4.02951989530937 1104 9 -1.8368762816131259 1104 10-11 -2.282738788988784 1104 12-13 -3.829386293531023 1104 14-15 -1.9191472270419645 1104 16-17 -5.29475717995455 1104 18-19 -2.3393147422752705 1104 20-21 -2.756013832658571 1104 22-23 -2.6426068083962733 1104 24-25 -1.9565114709851592 1104 26-27 -5.552499145591252 1104 28-29 -0.4561524002313426 1104 30-31 -0.651490432082543 1104 32-33 -6.70677681236892 1104 34-35 -2.7530741188635908 1104 36-37 -4.460806627582944 1104 38-39 -4.533362762968029 1104 40-41 -5.033133752212699 1104 42-43 -3.4534525781236276 1104 44-45 -4.666433274328011 1104 46-47 -1.5421116529669163 1104 48-49 -3.8647969355206158 1104 50-51 -3.2696122590859495 1104 52-53 -1.191240776767085 1104 54-55 -3.2259948384948416 1104 56-57 -3.168027988093776 1104 58-59 -4.441696023274968 1104 60-61 -0.7758079639658639 1104 62-63 -1.732452671597411 1104 64-65 -0.9311723729486836 1104 66-67 0.2019655782813672 1104 68-69 -3.6712504965794395 1104 70-71 -0.7261233284259596 1104 72-73 -3.4548464328069564 1104 74-75 -4.236444038417725 1104 76 -1.1610503531556162 1105 1 -2.7017372977899328 1105 2 3.089383277541174 1105 3 2.320439867150398 1105 4 3.5815856657961938 1105 5 5.630312450707191 1105 6 2.620055018081331 1105 7 -2.876250211776526 1105 8 6.404046538257063 1105 9 6.3449419002050576 1105 10-11 4.063415057165063 1105 12-13 3.212571748427017 1105 14-15 3.9654681575734223 1105 16-17 1.2262218410244685 1105 18-19 1.5942516912911628 1105 20-21 1.999230922586186 1105 22-23 4.318931653142187 1105 24-25 3.5190130045393175 1105 26-27 3.765682672590568 1105 28-29 4.536854592775651 1105 30-31 2.3589990784069705 1105 32-33 3.2232931177010116 1105 34-35 3.6140587482692723 1105 36-37 5.8748577080813895 1105 38-39 4.603000873395608 1105 40-41 3.3025305834516345 1105 42-43 3.735358610687559 1105 44-45 4.791608683713946 1105 46-47 4.531314920459657 1105 48-49 3.6352030644793842 1105 50-51 1.6569611674874771 1105 52-53 0.6584095728832651 1105 54-55 4.340438727938725 1105 56-57 2.3879160678502807 1105 58-59 3.624737543158595 1105 60-61 1.4759402877823895 1105 62-63 2.945869006724269 1105 64-65 3.914981473205163 1105 66-67 -0.7176148412990528 1105 68-69 2.486092160763217 1105 70-71 2.700450098147467 1105 72-73 5.03466405670353 1105 74-75 1.4383811364074504 1105 76 1.3844041922989305 1106 1 2.1618990658464305 1106 2 5.653019641177536 1106 3 3.2113489580594887 1106 4 5.081585665796194 1106 5 -0.9787784583837187 1106 6 3.620055018081331 1106 7 3.86920433367802 1106 8 1.8858647200752472 1106 9 6.963123718386875 1106 10-11 5.113415057165064 1106 12-13 4.389844475699743 1106 14-15 3.0154681575734195 1106 16-17 5.889858204660836 1106 18-19 7.926069873109341 1106 20-21 5.4901400134952745 1106 22-23 6.5189316531421895 1106 24-25 5.928103913630228 1106 26-27 3.5475008544087494 1106 28-29 5.932309138230199 1106 30-31 7.890817260225152 1106 32-33 2.877838572246464 1106 34-35 1.9276951119056385 1106 36-37 2.9930395262632103 1106 38-39 4.666637237031971 1106 40-41 4.920712401633452 1106 42-43 2.908085883414831 1106 44-45 0.014335956441218656 1106 46-47 1.8040421931869304 1106 48-49 6.535203064479383 1106 50-51 7.484233894760205 1106 52-53 6.53568230015599 1106 54-55 3.5086205461205466 1106 56-57 5.874279704213919 1106 58-59 1.9429193613404152 1106 60-61 6.939576651418754 1106 62-63 4.986778097633362 1106 64-65 2.3649814732051624 1106 66-67 5.759657885973674 1106 68-69 3.963364888035944 1106 70-71 7.677722825420194 1106 72-73 3.9528458748853517 1106 74-75 4.552017500043814 1106 76 1.7389496468443824 1107 1 -3.5047676008202373 1107 2 2.203019641177537 1107 3 -2.921984375273844 1107 4 -2.5017476675371384 1107 5 -0.11211179171705155 1107 6 1.620055018081331 1107 7 1.3358710003446888 1107 8 -0.5808019465914214 1107 9 1.7464570517202063 1107 10-11 0.5634150571650629 1107 12-13 -0.7684888576335922 1107 14-15 -0.2428651757599134 1107 16-17 -0.5768084620058325 1107 18-19 -3.0989301268906573 1107 20-21 0.17347334682860804 1107 22-23 0.6105983198088545 1107 24-25 1.9281039136302276 1107 26-27 -0.8441658122579163 1107 28-29 1.4989758048968618 1107 30-31 -2.0425160731081817 1107 32-33 -1.113828094420203 1107 34-35 -0.730638221427693 1107 36-37 0.5680395262632096 1107 38-39 -0.15836276296802865 1107 40-41 -3.170954265033213 1107 42-43 1.1330858834148323 1107 44-45 -0.5189973768921163 1107 46-47 -0.4042911401464053 1107 48-49 -0.8231302688539479 1107 50-51 -1.9490994385731284 1107 52-53 -1.947651033177344 1107 54-55 -1.383046120546119 1107 56-57 -4.184053629119415 1107 58-59 1.6512526946737474 1107 60-61 3.797909984752085 1107 62-63 -1.171555235699973 1107 64-65 -1.960018526794837 1107 66-67 -3.0320087806929905 1107 68-69 -3.1199684452973884 1107 70-71 -1.0306105079131385 1107 72-73 -3.4804874584479855 1107 74-75 -2.781315833289522 1107 76 -4.74438368648895 1108 1 -1.5047676008202373 1108 2 -0.2969803588224629 1108 3 1.3280156247261559 1108 4 -2.2517476675371384 1108 5 0.9712215416162806 1108 6 1.5367216847479988 1108 7 2.5858710003446888 1108 8 2.0025313867419143 1108 9 -1.3368762816131259 1108 10-11 0.021748390498398606 1108 12-13 3.273177809033079 1108 14-15 2.0904681575734223 1108 16-17 3.1315248713274997 1108 18-19 2.0260698731093427 1108 20-21 -1.159859986504724 1108 22-23 -3.7227350135244777 1108 24-25 0.5531039136302276 1108 26-27 -2.385832478924584 1108 28-29 -3.1676908617698025 1108 30-31 -0.12584940644151388 1108 32-33 1.9278385722464648 1108 34-35 3.1443617785723035 1108 36-37 0.15137285959654534 1108 38-39 0.00830390369863565 1108 40-41 3.162379068300119 1108 42-43 1.0080858834148323 1108 44-45 0.10600262310788366 1108 46-47 0.4707088598535982 1108 48-49 -1.4897969355206158 1108 50-51 0.9675672280935359 1108 52-53 -0.072651033177344 1108 54-55 0.20028721278721306 1108 56-57 4.565946370880582 1108 58-59 -0.9737473053262491 1108 60-61 1.3812433180854207 1108 62-63 0.953444764300027 1108 64-65 -1.7933518601281726 1108 66-67 -1.2403421140263262 1108 68-69 3.463364888035944 1108 70-71 3.3027228254201937 1108 72-73 1.186179208218686 1108 74-75 0.9270175000438137 1108 76 -2.911050353155616 1109 1 -2.5523866484392848 1109 2 -5.189837501679605 1109 3 -4.94579389908337 1109 4 -2.3469857627752333 1109 5 3.364078684473423 1109 6 -0.45137355334724205 1109 7 -1.6165099520362638 1109 8 3.0858647200752465 1109 9 1.234552289815447 1109 10-11 -2.7580135142635065 1109 12-13 -3.345869810014541 1109 14-15 -0.8916746995694353 1109 16-17 -1.37442750962488 1109 18-19 -0.6167872697477996 1109 20-21 -1.3741457007904394 1109 22-23 -1.859639775429244 1109 24-25 0.1781039136302276 1109 26-27 -4.838213431305537 1109 28-29 0.04659485251591278 1109 30-31 -0.24489702548913428 1109 32-33 -0.4293042848963893 1109 34-35 1.9062665404770698 1109 36-37 -2.092674759451075 1109 38-39 -1.2119341915394592 1109 40-41 -2.0221447412236913 1109 42-43 0.2580858834148323 1109 44-45 -0.8999497578444959 1109 46-47 -1.338814949670212 1109 48-49 -2.6147969355206158 1109 50-51 -0.6157661052397962 1109 52-53 -2.3286034141297236 1109 54-55 -0.019950882450885388 1109 56-57 -1.797148867214652 1109 58-59 -1.8427949243738695 1109 60-61 -0.9461376342955319 1109 62-63 -2.2846504737952102 1109 64-65 -2.585018526794837 1109 66-67 -1.0974849711691839 1109 68-69 -5.893777969106914 1109 70-71 -0.679420031722664 1109 72-73 1.2457030177424926 1109 74-75 0.19487464290095602 1109 76 1.6246639325586685 1110 1 -3.519919115971753 1110 2 -4.728798540640646 1110 3 0.22953077624130458 1110 4 -2.509323425112896 1110 5 -2.1878693674746295 1110 6 -1.470854072827759 1110 7 0.8510225154962043 1110 8 -3.3232261890156636 1110 9 -2.927785372522216 1110 10-11 -1.618403124653117 1110 12-13 -1.8328827970275299 1110 14-15 0.9200136121188756 1110 16-17 -2.0465054317028013 1110 18-19 -4.496657399617931 1110 20-21 -2.591678168322904 1110 22-23 -5.181068346857813 1110 24-25 0.06446754999386073 1110 26-27 -2.5070446001367053 1110 28-29 -4.735872679951621 1110 30-31 -4.004637285229396 1110 32-33 -1.0494341550262618 1110 34-35 -1.1132139790034543 1110 36-37 -2.397869564645884 1110 38-39 -6.533362762968029 1110 40-41 -0.7429239620029087 1110 42-43 -2.764641389312441 1110 44-45 -0.9811185890133274 1110 46-47 0.8494967386414771 1110 48-49 1.271566700843021 1110 50-51 0.7933248038511103 1110 52-53 -0.9779540634803716 1110 54-55 -3.8868339993340015 1110 56-57 0.34246152239573746 1110 58-59 -0.6479897295686783 1110 60-61 -2.6149688031267004 1110 62-63 -2.6450400841848243 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2.5958767840346795 2117 3 5.125634672345203 2117 4 0.5815856657961938 2117 5 3.435507255901996 2117 6 7.191483589509904 2117 7 -0.6165099520362638 2117 8 3.728721862932389 2117 9 0.7345522898154471 2117 10-11 9.991986485736494 2117 12-13 6.725558761414028 2117 14-15 8.751182443287707 2117 16-17 7.518429633232262 2117 18-19 3.6332127302522004 2117 20-21 -0.1955742722190088 2117 22-23 8.676074510285044 2117 24-25 5.285246770773085 2117 26-27 4.661786568694463 2117 28-29 1.7965948525159128 2117 30-31 -2.030611311203419 2117 32-33 2.177838572246465 2117 34-35 2.5491236833342086 2117 36-37 1.4787538119774943 2117 38-39 2.323780094174829 2117 40-41 3.1207124016334546 2117 42-43 2.2223715977005476 2117 44-45 4.921478813584077 2117 46-47 6.232613621758361 2117 48-49 6.563774493050815 2117 50-51 4.241376751903058 2117 52-53 1.3142537287274223 2117 54-55 3.7300491175491146 2117 56-57 5.095708275642487 2117 58-59 2.800062218483273 2117 60-61 1.1252909371330375 2117 62-63 2.393920954776217 2117 64-65 7.700695758919448 2117 66-67 3.509657885973674 2117 68-69 3.713364888035944 2117 70-71 1.4277228254201937 2117 72-73 2.4242744463139196 2117 74-75 3.2305889286152443 2117 76 2.838949646844384 2118 1 -1.4809580770107118 2118 2 -0.9755517873938899 2118 3 -3.160079613369085 2118 4 2.010157094367621 2118 5 4.2926501130448536 2118 6 1.9057693037956156 2118 7 0.8120614765351633 2118 8 4.0144361486466735 2118 9 2.163123718386874 2118 10-11 5.634843628593632 2118 12-13 1.511273047128313 2118 14-15 -1.3916746995694318 2118 16-17 -0.981570366767734 2118 18-19 1.418927015966485 2118 20-21 4.875854299209561 2118 22-23 4.604645938856471 2118 24-25 -1.500467514941203 2118 26-27 -1.2667848598769673 2118 28-29 -2.7034051474840872 2118 30-31 -1.8877541683462802 2118 32-33 2.3206957151036107 2118 34-35 -2.022304888094361 2118 36-37 -4.592674759451077 2118 38-39 3.1809229513176867 2118 40-41 -2.0935733126522607 2118 42-43 -2.9204855451565983 2118 44-45 -3.7928069007016383 2118 46-47 -2.767386378241639 2118 48-49 -5.1505112212349005 2118 50-51 0.2413767519030614 2118 52-53 1.957110871584561 2118 54-55 -0.4128080253080242 2118 56-57 -1.547148867214652 2118 58-59 1.0143479327689882 2118 60-61 4.1252909371330375 2118 62-63 0.5367780976333592 2118 64-65 4.129267187490878 2118 66-67 1.1525150288308161 2118 68-69 1.1419363166073708 2118 70-71 -3.7865628888655216 2118 72-73 3.638560160599635 2118 74-75 0.6591603571866713 2118 76 2.1246639325586685 2119 1 -0.33810093415356945 2119 2 -6.213647025489129 2119 3 -4.088651041940512 2119 4 0.08158566579619375 2119 5 -7.612111791717053 2119 6 -1.7132783152520012 2119 7 -5.164128999655311 2119 8 -2.9141352799247535 2119 9 -0.670209614946458 2119 10-11 -0.2699182761682728 2119 12-13 -4.5601555243002565 2119 14-15 -3.5345318424265777 2119 16-17 0.2565248713274997 2119 18-19 0.10940320644267487 2119 20-21 -1.659859986504724 2119 22-23 -5.681068346857813 2119 24-25 -3.3218960863697724 2119 26-27 -0.8858324789245842 2119 28-29 -5.001024195103135 2119 30-31 -0.29251607310818173 2119 32-33 1.1778385722464648 2119 34-35 -3.6889715547610287 2119 36-37 -4.140293807070126 2119 38-39 -3.2000294296346965 2119 40-41 0.37071240163345465 2119 42-43 -4.241914116585168 2119 44-45 1.3976692897745515 2119 46-47 -7.8626244734797375 2119 48-49 -1.28146360218728 2119 50-51 -5.782432771906464 2119 52-53 -0.0309843665106726 2119 54-55 6.491953879453881 2119 56-57 -3.2257202957860827 2119 58-59 -4.973747305326251 2119 60-61 0.2562433180854171 2119 62-63 -3.629888569033305 2119 64-65 -5.501685193461505 2119 66-67 -3.573675447359662 2119 68-69 -3.286635111964056 2119 70-71 -0.8222771745798063 2119 72-73 -0.8971541251146498 2119 74-75 -3.1979824999561863 2119 76 -3.161050353155616 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 7.0 13 37.0 14 123.0 15 270.0 16 301.0 17 413.0 18 466.0 19 457.0 20 355.0 21 295.0 22 206.0 23 138.0 24 93.0 25 79.0 26 56.0 27 44.0 28 30.0 29 32.0 30 27.0 31 32.0 32 27.0 33 31.0 34 42.0 35 37.0 36 61.0 37 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 7.956698240866035 28.33558863328823 9.93234100135318 53.77537212449256 2 4.25531914893617 22.919472124966333 42.95717748451387 29.868031241583626 3 3.79746835443038 44.7077834635066 23.75437651494748 27.74037166711554 4 4.120657150552114 27.578777269054672 20.657150552114192 47.643415028279016 5 3.0702935631564774 30.837597629948828 21.115001346619984 44.97710746027471 6 5.844330729868031 46.16213304605441 11.123081066523028 36.87045515755454 7 6.032857527605709 37.0320495556154 21.088068946943174 35.84702396983571 8 4.524643145704283 36.25100996498788 25.28952329652572 33.93482359278212 9 7.756531106921627 29.921896040937245 14.839752221922971 47.48182063021815 10-11 7.8373283059520595 31.160786426070565 26.286022084567733 34.71586318340964 12-13 6.988957716132507 26.70347427955831 26.4341502827902 39.87341772151899 14-15 11.338540263937517 31.120387826555344 20.024239159709133 37.516832749798006 16-17 14.39536762725559 31.349313223808238 20.17236735793159 34.082951791004575 18-19 11.39240506329114 27.875033665499593 19.243199569081604 41.48936170212766 20-21 10.113116078642607 28.53487745758147 20.14543495825478 41.20657150552115 22-23 16.576892001077294 28.77726905467277 16.99434419606787 37.65149474818206 24-25 12.079181255049825 34.931322380824135 15.997845408025855 36.99165095610019 26-27 11.338540263937517 34.648532184217615 20.1992997576084 33.81362779423647 28-29 13.196875841637489 30.2854834365742 24.64314570428225 31.87449501750606 30-31 13.035281443576624 29.679504443845943 25.45111769458659 31.834096417990843 32-33 9.762994882844062 35.07945057904659 23.57931591704821 31.57823862106114 34-35 9.385941287368704 36.291408564503094 20.57635335308376 33.74629679504444 36-37 10.867223269593321 30.824131430110423 23.767842714785886 34.54080258551037 38-39 11.486668462159978 25.922434688930785 27.134392674387286 35.45650417452195 40-41 9.762994882844062 27.080527875033667 26.393751683274978 36.76272555884729 42-43 6.032857527605709 25.46458389442499 29.302450848370594 39.20010772959871 44-45 7.271747912739025 25.329921896040936 31.60517102073795 35.79315917048209 46-47 7.729598707244816 29.94882844061406 26.851602477780773 35.46997037436036 48-49 7.460274710476704 33.55776999730676 22.326959332076488 36.65499596014005 50-51 7.460274710476704 33.23458120118502 19.754915162941018 39.55022892539725 52-53 6.140587126312955 32.332345812011845 22.95987072448155 38.56719633719364 54-55 6.1809857258281715 29.652572044169133 21.599784540802585 42.56665768920011 56-57 10.570966873148398 30.729868031241587 19.970374360355507 38.728790735254506 58-59 9.49367088607595 30.851063829787233 18.179369781847562 41.475895502289255 60-61 7.325612712092648 27.95583086453003 20.010772959870724 44.7077834635066 62-63 9.345542687853488 28.89846485321842 19.458658766496097 42.29733369243199 64-65 10.032318879612173 30.608672232695934 16.07864260705629 43.280366280635604 66-67 7.971990304336115 36.91085375706976 13.546997037436034 41.570158901158095 68-69 9.103151090762186 36.27794236466469 15.903582009157017 38.71532453541611 70-71 8.470239698357124 39.92728252087261 15.822784810126583 35.77969297064369 72-73 8.295179100457851 36.21061136547266 19.09507137085914 36.399138163210345 74-75 7.71613250740641 39.40210072717479 14.947481820630218 37.93428494478858 76 6.5984379208187445 36.089415566927016 14.651225424185297 42.660921088068946 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.5 16 2.0 17 3.0 18 2.0 19 2.0 20 4.5 21 6.0 22 9.5 23 12.0 24 10.0 25 9.0 26 13.0 27 18.0 28 22.5 29 26.0 30 28.0 31 37.5 32 54.5 33 64.0 34 66.0 35 77.5 36 99.0 37 111.0 38 119.0 39 147.0 40 169.0 41 185.5 42 200.0 43 217.0 44 239.5 45 230.5 46 216.0 47 212.0 48 209.5 49 213.5 50 216.0 51 207.5 52 182.5 53 156.5 54 147.0 55 135.0 56 121.5 57 101.0 58 82.0 59 74.5 60 65.0 61 53.5 62 44.0 63 45.0 64 38.5 65 28.5 66 23.5 67 21.0 68 16.0 69 9.5 70 9.0 71 10.0 72 7.0 73 3.5 74 4.0 75 5.0 76 4.0 77 3.0 78 3.0 79 3.0 80 2.0 81 0.5 82 0.0 83 0.0 84 1.5 85 1.5 86 0.5 87 1.0 88 1.5 89 2.0 90 2.5 91 2.5 92 2.0 93 1.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4847831941826017 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 3713.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.0608672232696 #Duplication Level Percentage of deduplicated Percentage of total 1 98.5718209283164 96.66038244007541 2 1.098599285910464 2.1545919741448962 3 0.21971985718209283 0.6463775922434689 4 0.0 0.0 5 0.10985992859104642 0.5386479935362241 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA 5 0.13466199838405601 No Hit TGCCTAGATGTCACTTATTAATAAGAAATAGTTCTTTTCTCCACCTGTCT 5 0.13466199838405601 No Hit TTTTATTACTTATGTTTTCTTGTTTTTATGTCATTTTCATTTTCTCAGTA 5 0.13466199838405601 No Hit CCATAGTCTGATCTAAGATATGACTTCTCACTCAGAACCTGTCTCTTATA 5 0.13466199838405601 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.026932399676811204 0.0 2 0.0 0.0 0.0 0.026932399676811204 0.0 3 0.0 0.0 0.0 0.08079719903043361 0.0 4 0.0 0.0 0.0 0.08079719903043361 0.0 5 0.0 0.0 0.0 0.10772959870724481 0.0 6 0.0 0.0 0.0 0.10772959870724481 0.0 7 0.0 0.0 0.0 0.10772959870724481 0.0 8 0.0 0.0 0.0 0.10772959870724481 0.0 9 0.0 0.0 0.0 0.10772959870724481 0.0 10 0.0 0.0 0.0 0.13466199838405601 0.0 11 0.0 0.0 0.0 0.13466199838405601 0.0 12 0.0 0.0 0.0 0.16159439806086723 0.0 13 0.0 0.0 0.0 0.16159439806086723 0.0 14 0.0 0.0 0.0 0.16159439806086723 0.0 15 0.0 0.0 0.0 0.16159439806086723 0.0 16 0.0 0.0 0.0 0.18852679773767841 0.0 17 0.0 0.0 0.0 0.18852679773767841 0.0 18 0.0 0.0 0.0 0.40398599515216804 0.0 19 0.0 0.0 0.0 0.5386479935362241 0.0 20 0.0 0.0 0.0 0.5925127928898465 0.0 21 0.0 0.0 0.0 0.5925127928898465 0.0 22 0.0 0.0 0.0 0.6463775922434689 0.0 23 0.0 0.0 0.0 0.7002423915970913 0.0 24 0.0 0.0 0.0 0.7810395906275249 0.0 25 0.0 0.0 0.0 0.7810395906275249 0.0 26 0.0 0.0 0.0 0.7810395906275249 0.0 27 0.0 0.0 0.0 0.8349043899811474 0.0 28 0.0 0.0 0.0 0.8349043899811474 0.0 29 0.0 0.0 0.0 0.8887691893347698 0.0 30 0.0 0.0 0.0 0.8887691893347698 0.0 31 0.0 0.0 0.0 0.8887691893347698 0.0 32 0.0 0.0 0.0 0.9695663883652034 0.0 33 0.0 0.0 0.0 1.0234311877188258 0.0 34 0.0 0.0 0.0 1.050363587395637 0.0 35 0.0 0.0 0.0 1.2388903851333153 0.0 36 0.0 0.0 0.0 1.5351467815782387 0.0 37 0.0 0.0 0.0 1.723673579315917 0.0 38 0.0 0.0 0.0 2.100727174791274 0.0 39 0.0 0.0 0.0 2.6932399676811203 0.0 40 0.0 0.0 0.0 3.0164287638028546 0.0 41 0.0 0.0 0.0 3.0972259628332885 0.0 42 0.0 0.0 0.0 3.2588203608941555 0.0 43 0.0 0.0 0.0 3.474279558308645 0.0 44 0.0 0.0 0.0 3.689738755723135 0.0 45 0.0 0.0 0.0 4.228386749259359 0.0 46 0.0 0.0 0.0 4.4977107460274715 0.0 47 0.0 0.0 0.0 4.605440344734716 0.0 48 0.0 0.0 0.0 4.874764341502828 0.0 49 0.0 0.0 0.0 5.063291139240507 0.0 50 0.0 0.0 0.0 5.305682736331807 0.0 51 0.0 0.0 0.0 5.413412335039052 0.0 52 0.0 0.0 0.0 5.467277134392674 0.0 53 0.0 0.0 0.0 5.548074333423108 0.0 54 0.0 0.0 0.0 5.7366011311607865 0.0 55 0.0 0.0 0.0 5.952060328575276 0.0 56 0.0 0.0 0.0 5.978992728252087 0.0 57 0.0 0.0 0.0 5.978992728252087 0.0 58 0.0 0.0 0.0 6.005925127928898 0.0 59 0.0 0.0 0.0 6.221384325343388 0.0 60 0.0 0.0 0.0 6.302181524373822 0.0 61 0.0 0.0 0.0 6.409911123081066 0.0 62 0.0 0.0 0.0 6.4907083221115 0.0 63 0.0 0.0 0.0 6.598437920818745 0.0 64 0.0 0.0 0.0 6.598437920818745 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE