FastQCFastQC Report
Wed 25 May 2016
SRR1780662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT131735.46997037436036No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT43211.63479666038244RNA PCR Primer, Index 47 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1494.01292755184487No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC982.639375168327498No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT651.7506059789927284No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC591.5890115809318612No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT511.3735523835173715No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT381.0234311877188258No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT360.9695663883652034No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC350.9426339886883921No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT240.6463775922434689No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGGTAGATCTCGTATGCCGT230.6194451925666576No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC160.43091839482897926No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGTCTT150.40398599515216804No Hit
CTTATACACATCTCCGAGCCCACGCGACAATGGTAGATCTCGTATGCCGT140.37705359547535683No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGTCTT140.37705359547535683No Hit
ACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCT140.37705359547535683RNA PCR Primer, Index 47 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC140.37705359547535683No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGTCTT120.32318879612173446No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCC110.29625639644492324No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCC110.29625639644492324No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTCTGCCGTCTT100.26932399676811203No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATTTCGTATGCCGT100.26932399676811203No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCG90.24239159709130084No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCC90.24239159709130084No Hit
ATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCTT90.24239159709130084RNA PCR Primer, Index 23 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT90.24239159709130084No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC90.24239159709130084No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCACGTATGCCGT80.21545919741448963No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG80.21545919741448963No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCCCGTATGCCGT80.21545919741448963No Hit
CTTATAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG80.21545919741448963No Hit
CTTATACACATCTCCGAGCACACGAGACAATGGTAGATCTCGTATGCCGT80.21545919741448963No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT70.18852679773767841No Hit
CTTATACACATATCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT70.18852679773767841No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG70.18852679773767841No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGT60.16159439806086723No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAAATCGTATGCCGT60.16159439806086723No Hit
CTTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCGT60.16159439806086723No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTAGTATGCCGT60.16159439806086723No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTCGATCTCGTATGCCGT60.16159439806086723No Hit
CTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCTTGA60.16159439806086723RNA PCR Primer, Index 47 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCG60.16159439806086723No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACATCGTATGCCGT60.16159439806086723No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG60.16159439806086723No Hit
GTTCTGAGTGAGAAGTCATATCTTAGATCAGACTATGGCTGTCTCTTATA50.13466199838405601No Hit
GTGGAGAAAAGAACTATTTCTTATTAATAAGTGACATCTAGGCACTGTCT50.13466199838405601No Hit
CTTATACACATCTCCGAGACCACGAGACAATGGTAGATCTCGTATGCCGT50.13466199838405601No Hit
GTTAAATATTATATGTCTAAGTACTGAGAAAATGAAAATGACATAAAAAC50.13466199838405601No Hit
CTTATAAACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT50.13466199838405601No Hit
CTTAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC50.13466199838405601No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTCTGCCGT50.13466199838405601No Hit
ATACACATCTACGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT50.13466199838405601RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCG50.13466199838405601No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT50.13466199838405601No Hit
GCAGCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCAC50.13466199838405601No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGC50.13466199838405601No Hit
TATTGTGACTGGTCATTTCAACATTTTCAATTTTACCCACTCTCACTTGC40.10772959870724481No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAAATCTCGTATGCCGT40.10772959870724481No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGGTAGATCTCGTATGCCGT40.10772959870724481No Hit
CTTATACACATCTCCGAGCCCACGAGAAAATGGTAGATATCGTATGCCGT40.10772959870724481No Hit
CTACCAGGATTAATCACATGAACAGATCCAATCTGTTCATATTCTGTCTC40.10772959870724481No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCCCGTATGCCGTCTT40.10772959870724481No Hit
CTCTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG40.10772959870724481No Hit
CTCTTATACACATCTCCGAGCCCAAGAGACAATGGTAGATCTCGTATGCC40.10772959870724481No Hit
TCTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT40.10772959870724481No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGAACTCGTATGCCGT40.10772959870724481No Hit
ATACACATCTCCGAGCCCACGAGACACTGGTAGATCTCGTATGCCGTCTT40.10772959870724481RNA PCR Primer, Index 23 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA150.002053246170.0000133
GATATCG150.002053246170.0000136
TATCGTA150.002053246170.0000138
ATATCGT150.002053246170.0000137
TAGATAT150.002053246170.0000134
AGATATC150.002053246170.0000135
ACCATGG206.931956E-570.027
CCATGGT206.931956E-570.028
CATGGTA206.931956E-570.029
CGAGACC206.931956E-570.023
ATCGTAT206.931956E-570.039
AGACCAT206.931956E-570.025
GACCATG206.931956E-570.026
GAGACCA206.931956E-570.024
AAACCAA252.0921287E-456.00000468
TTATACA2550.053.529412
CTTATAC2550.053.529411
GGTAGAA200.00641956652.532
AAAACCA405.6440695E-752.567
TATACAC2700.049.259263
Produced by FastQC (version 0.11.5)