##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780662_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3713 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67196337193644 34.0 34.0 34.0 31.0 34.0 2 33.09803393482359 34.0 34.0 34.0 31.0 34.0 3 33.40506329113924 34.0 34.0 34.0 34.0 34.0 4 33.477511446269865 34.0 34.0 34.0 34.0 34.0 5 33.38082413143011 34.0 34.0 34.0 34.0 34.0 6 37.33046054403447 38.0 37.0 38.0 37.0 38.0 7 36.67573390789119 38.0 37.0 38.0 34.0 38.0 8 36.8505251817937 38.0 37.0 38.0 35.0 38.0 9 36.53595475356855 38.0 37.0 38.0 34.0 38.0 10-11 36.32534338809588 38.0 37.0 38.0 34.0 38.0 12-13 35.984917856180985 38.0 36.5 38.0 32.5 38.0 14-15 36.1089415566927 38.0 36.5 38.0 33.0 38.0 16-17 35.899542149205494 38.0 36.0 38.0 31.0 38.0 18-19 35.85119849178562 38.0 36.0 38.0 31.0 38.0 20-21 35.73081066523027 38.0 36.0 38.0 31.0 38.0 22-23 35.90762186910854 38.0 36.5 38.0 32.5 38.0 24-25 34.65674656611904 37.0 34.0 38.0 28.0 38.0 26-27 34.91987611096148 37.0 34.5 38.0 30.0 38.0 28-29 34.66900080797199 37.5 34.5 38.0 28.0 38.0 30-31 34.276999730676 37.0 34.0 38.0 25.0 38.0 32-33 35.04147589550229 37.0 35.5 38.0 27.0 38.0 34-35 34.56423377322919 37.0 35.0 38.0 27.5 38.0 36-37 34.136277942364664 37.0 34.0 38.0 25.0 38.0 38-39 29.975626178292487 34.0 26.5 37.0 11.0 38.0 40-41 32.510234311877184 36.5 30.5 37.0 18.0 38.0 42-43 34.69889577161325 37.0 34.5 38.0 28.5 38.0 44-45 35.25531914893617 37.0 35.5 38.0 29.0 38.0 46-47 35.3775922434689 38.0 36.0 38.0 31.0 38.0 48-49 34.94303797468355 37.5 35.0 38.0 29.0 38.0 50-51 34.81457042822515 37.0 34.5 38.0 28.0 38.0 52-53 34.34608133584702 37.0 34.0 38.0 25.5 38.0 54-55 34.87611096148667 37.0 35.5 38.0 27.0 38.0 56-57 34.963237274441155 37.0 35.5 38.0 27.0 38.0 58-59 34.47158631834097 37.0 34.0 38.0 26.5 38.0 60-61 34.15526528413682 37.0 34.0 38.0 25.0 38.0 62-63 34.96215997845408 37.0 35.0 38.0 29.0 38.0 64-65 35.027605709668734 37.0 36.0 38.0 29.0 38.0 66-67 32.82386210611365 37.0 31.0 38.0 21.5 38.0 68-69 29.71518987341772 34.0 24.0 37.5 11.0 38.0 70-71 25.988015082143818 28.5 16.5 35.5 10.5 37.5 72-73 21.074468085106382 21.5 11.0 29.0 10.5 35.5 74-75 20.49501750605979 21.0 11.0 27.0 11.0 36.0 76 21.108806894694318 21.0 11.0 29.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7273087292824059 1101 2 0.39001955646692466 1101 3 0.6803542948279784 1101 4 0.4123635136792956 1101 5 0.2978696741854634 1101 6 0.2341005485742258 1101 7 0.8347737058263291 1101 8 1.0227677293466755 1101 9 1.4120452646768413 1101 10-11 1.1226048074732233 1101 12-13 1.5992134327660565 1101 14-15 1.7534084336715878 1101 16-17 0.48773083641504655 1101 18-19 0.9181512931512898 1101 20-21 1.7330179688732343 1101 22-23 1.1400334679939874 1101 24-25 -1.6245858673490332 1101 26-27 -0.10161172891436365 1101 28-29 1.1278678558283843 1101 30-31 1.2189407449275862 1101 32-33 2.5538103709156346 1101 34-35 0.16159023724812727 1101 36-37 0.42121058765795283 1101 38-39 1.2097319347319377 1101 40-41 4.535037221550368 1101 42-43 1.7659724047881866 1101 44-45 1.8742253360674397 1101 46-47 2.631604908249635 1101 48-49 1.0169856824462187 1101 50-51 1.7618578789631343 1101 52-53 2.652883337422807 1101 54-55 -2.0488333669254715 1101 56-57 -2.992042277897532 1101 58-59 -0.8261301929065112 1101 60-61 -0.9844086103954481 1101 62-63 -0.6478247994695323 1101 64-65 0.869588635049162 1101 66-67 -3.413655788655795 1101 68-69 -1.5674499111999083 1101 70-71 0.6687809266756695 1101 72-73 2.738148145713936 1101 74-75 0.385392093286832 1101 76 -1.6492700282173978 1102 1 0.10230872928240586 1102 2 0.5150195564669247 1102 3 0.6803542948279784 1102 4 0.4123635136792956 1102 5 0.2978696741854634 1102 6 0.6431914576651323 1102 7 -0.7674990214463975 1102 8 0.9659495475284956 1102 9 0.7188634464950212 1102 10-11 0.4862411711095831 1102 12-13 -0.19055929450667008 1102 14-15 0.07727207003522807 1102 16-17 1.2206853818695933 1102 18-19 1.0942876567876496 1102 20-21 2.0682452416005077 1102 22-23 0.992306195266714 1102 24-25 -4.243904049167213 1102 26-27 -0.8402480925507234 1102 28-29 0.002867855828384336 1102 30-31 2.656440744927586 1102 32-33 1.139037643642908 1102 34-35 2.4286356917935805 1102 36-37 -4.65265304870568 1102 38-39 -5.068677156177156 1102 40-41 -0.15246277844963174 1102 42-43 2.41938149569728 1102 44-45 1.146952608794713 1102 46-47 1.7452412718859946 1102 48-49 -2.761423408462875 1102 50-51 0.6766306062358609 1102 52-53 -0.47211666257719287 1102 54-55 1.235257542165435 1102 56-57 2.1840940857388276 1102 58-59 -0.2579483747246982 1102 60-61 -6.711681337668175 1102 62-63 -4.0057793449240755 1102 64-65 1.3411795441400685 1102 66-67 3.671571484071471 1102 68-69 0.7848228160728183 1102 70-71 -0.6039463460516039 1102 72-73 -1.057306399740611 1102 74-75 0.06721027510501187 1102 76 -1.8197245736719445 1103 1 1.1023087292824059 1103 2 0.5150195564669247 1103 3 0.09702096149464268 1103 4 0.16236351367929558 1103 5 0.2978696741854634 1103 6 0.7341005485742258 1103 7 0.7931070391596649 1103 8 0.8977677293466755 1103 9 0.620378598010177 1103 10-11 -2.2107285258601053 1103 12-13 -1.3382865672339435 1103 14-15 0.6492417670049164 1103 16-17 1.4252308364150466 1103 18-19 0.9389846264846184 1103 20-21 0.33718463553990574 1103 22-23 1.3067001346606588 1103 24-25 -4.395419200682369 1103 26-27 -0.5599450622476922 1103 28-29 0.41953452249504863 1103 30-31 2.531440744927586 1103 32-33 1.5746437042489632 1103 34-35 1.3907569039147916 1103 36-37 -0.9746227456753829 1103 38-39 0.8555652680652734 1103 40-41 0.014203888217039662 1103 42-43 -0.12986092854514197 1103 44-45 2.3742253360674397 1103 46-47 2.673271574916299 1103 48-49 -1.2121809842204527 1103 50-51 1.3243578789631343 1103 52-53 1.2362166707561357 1103 54-55 -1.0071667002588 1103 56-57 1.3204577221024678 1103 58-59 1.0488698070934888 1103 60-61 -5.567741943728784 1103 62-63 -1.0853247994695323 1103 64-65 0.8904219683824977 1103 66-67 -0.3094891219891238 1103 68-69 -0.4424499111999083 1103 70-71 -0.16455240665766624 1103 72-73 -3.803518520952732 1103 74-75 1.385392093286832 1103 76 0.22572997178260223 1104 1 0.8715394985131724 1104 2 0.5150195564669247 1104 3 0.6803542948279784 1104 4 0.10467120598698898 1104 5 0.2978696741854634 1104 6 0.1956390101126857 1104 7 0.6520813981340225 1104 8 0.9939215755005222 1104 9 1.5370452646768413 1104 10-11 0.6226048074732233 1104 12-13 1.4213288173814433 1104 14-15 1.3639853567485076 1104 16-17 0.13676929795350645 1104 18-19 0.7691128316128228 1104 20-21 1.2185948919501541 1104 22-23 1.2265719295324473 1104 24-25 0.1302218249586602 1104 26-27 1.331080578777943 1104 28-29 3.1567140096745376 1104 30-31 0.9256715141583598 1104 32-33 1.9432334478387148 1104 34-35 1.5510133141712075 1104 36-37 2.637556741504106 1104 38-39 2.714539627039631 1104 40-41 2.270614144627288 1104 42-43 1.4053954817112668 1104 44-45 0.8742253360674397 1104 46-47 0.4200664467111679 1104 48-49 0.9448702978308319 1104 50-51 2.295511725116981 1104 52-53 2.1048064143458873 1104 54-55 2.480012786920682 1104 56-57 1.8589192605640008 1104 58-59 2.4238698070934888 1104 60-61 -0.44594707193390803 1104 62-63 -0.6526324917772257 1104 64-65 2.0859347888953153 1104 66-67 2.514228826728818 1104 68-69 2.442165473415475 1104 70-71 1.8995501574448994 1104 72-73 -3.3195441619783708 1104 74-75 -3.7684540605593213 1104 76 -0.5050392589866277 1105 1 1.1023087292824059 1105 2 0.42411046555783116 1105 3 0.6803542948279784 1105 4 0.3214544227702021 1105 5 -0.24758487126908335 1105 6 0.37046418493785893 1105 7 0.8688646149172357 1105 8 -0.0340504524715044 1105 9 1.4461361737677478 1105 10-11 0.7135138983823168 1105 12-13 0.08216797822060329 1105 14-15 0.8045447973079547 1105 16-17 0.1297762909604998 1105 18-19 1.0488331113331029 1105 20-21 1.1136997870550545 1105 22-23 -0.23496653200601259 1105 24-25 0.16518685992369342 1105 26-27 0.8415700892674494 1105 28-29 1.3210496740102045 1105 30-31 0.9746225631094063 1105 32-33 0.27540128000654107 1105 34-35 -5.207727944570053 1105 36-37 -1.7890166850693205 1105 38-39 -1.4323135198135155 1105 40-41 1.3020826760958215 1105 42-43 -0.03516395884817314 1105 44-45 1.0560435178856196 1105 46-47 1.0634230900678219 1105 48-49 0.5567584097189453 1105 50-51 1.812994242599494 1105 52-53 0.4824287919682604 1105 54-55 -1.582924276016378 1105 56-57 -0.7249968233520789 1105 58-59 0.10568798891166864 1105 60-61 1.5610459350590986 1105 62-63 -3.596688435833169 1105 64-65 -1.5224568194962913 1105 66-67 -2.9193376068376153 1105 68-69 -4.351540820290815 1105 70-71 -1.603946346051604 1105 72-73 3.1245117820775725 1105 74-75 0.6581193660141054 1105 76 1.6348208808735123 1106 1 1.0023087292824044 1106 2 0.2150195564669275 1106 3 0.6803542948279784 1106 4 -0.28763648632070726 1106 5 0.2978696741854634 1106 6 0.034100548574222955 1106 7 -2.540226294173671 1106 8 0.5477677293466741 1106 9 -0.16295473532316151 1106 10-11 0.9726048074732176 1106 12-13 1.636713432766058 1106 14-15 -0.4090915663284136 1106 16-17 1.275230836415048 1106 18-19 0.6306512931512884 1106 20-21 -1.6044820311267642 1106 22-23 0.8150334679939846 1106 24-25 -1.1120858673490304 1106 26-27 0.7233882710856392 1106 28-29 1.0028678558283843 1106 30-31 1.6564407449275862 1106 32-33 -0.9836896290843598 1106 34-35 1.7740902372481244 1106 36-37 3.6337105876579585 1106 38-39 2.122231934731939 1106 40-41 2.1975372215503626 1106 42-43 1.0284724047881895 1106 44-45 2.1242253360674397 1106 46-47 1.2316049082496363 1106 48-49 1.8794856824462158 1106 50-51 0.7993578789631286 1106 52-53 0.72788333742281 1106 54-55 -2.1238333669254743 1106 56-57 0.8204577221024678 1106 58-59 0.3738698070934916 1106 60-61 1.6155913896045533 1106 62-63 1.9896752005304705 1106 64-65 0.1320886350491648 1106 66-67 2.2988442113442034 1106 68-69 4.207550088800097 1106 70-71 5.518780926675671 1106 72-73 2.0881481457139373 1106 74-75 1.9853920932868334 1106 76 5.125729971782601 1107 1 0.018975395949070162 1107 2 -0.06831377686641105 1107 3 0.6803542948279784 1107 4 -0.17096981965404012 1107 5 0.047869674185463396 1107 6 0.4841005485742258 1107 7 0.7931070391596649 1107 8 -0.8522322706533245 1107 9 1.2037119313435056 1107 10-11 0.03927147413988763 1107 12-13 0.9950467660993851 1107 14-15 0.3575751003382521 1107 16-17 0.38356416974838226 1107 18-19 1.8556512931512898 1107 20-21 1.753851302206563 1107 22-23 0.973366801327316 1107 24-25 0.4379141326509668 1107 26-27 -0.5599450622476922 1107 28-29 1.4195345224950557 1107 30-31 1.1147740782609148 1107 32-33 1.3663103709156346 1107 34-35 2.1407569039147916 1107 36-37 3.3170439209912814 1107 38-39 -0.26943473193472656 1107 40-41 -1.1941294451162996 1107 42-43 1.7868057381215152 1107 44-45 0.9575586694007754 1107 46-47 0.6732715749163063 1107 48-49 1.621152349112883 1107 50-51 1.8243578789631343 1107 52-53 1.6528833374228071 1107 54-55 2.4094999664078642 1107 56-57 2.1537910554357964 1107 58-59 2.0072031404268245 1107 60-61 0.7655913896045519 1107 62-63 -0.7936581328028609 1107 64-65 1.0987553017158334 1107 66-67 1.815510878010869 1107 68-69 3.0575500888000917 1107 70-71 -2.0395524066576627 1107 72-73 -2.0535185209527285 1107 74-75 0.510392093286832 1107 76 0.14239663844927009 1108 1 0.43564206261574157 1108 2 0.5150195564669247 1108 3 0.6803542948279784 1108 4 0.4123635136792956 1108 5 0.2978696741854634 1108 6 0.6507672152408901 1108 7 0.45977370582632915 1108 8 -0.18556560398666022 1108 9 0.7870452646768413 1108 10-11 1.205938140806552 1108 12-13 1.7867134327660565 1108 14-15 1.3159084336715878 1108 16-17 -1.1581024969182891 1108 18-19 0.18898462648461845 1108 20-21 1.4205179688732343 1108 22-23 0.8900334679939874 1108 24-25 3.187914132650967 1108 26-27 1.0650549377523078 1108 28-29 0.9612011891617129 1108 30-31 0.9897740782609148 1108 32-33 2.032977037582306 1108 34-35 2.2240902372481273 1108 36-37 2.233710587657953 1108 38-39 5.522231934731938 1108 40-41 2.9725372215503683 1108 42-43 1.745139071454851 1108 44-45 2.2075586694007683 1108 46-47 2.423271574916299 1108 48-49 2.24615234911289 1108 50-51 1.6993578789631343 1108 52-53 1.1945500040894714 1108 54-55 0.36783329974119283 1108 56-57 0.11212438876913922 1108 58-59 1.9238698070934888 1108 60-61 3.432258056271216 1108 62-63 2.581341867197132 1108 64-65 2.307088635049162 1108 66-67 3.940510878010869 1108 68-69 3.849216755466763 1108 70-71 1.6687809266756695 1108 72-73 2.9048148123806037 1108 74-75 -0.15627457337983586 1108 76 1.39239663844927 1109 1 -0.32626269928902474 1109 2 -1.77069472924736 1109 3 0.2517828662565478 1109 4 -0.516207914892135 1109 5 0.012155388471178696 1109 6 0.1626719771456564 1109 7 -1.2545120084593862 1109 8 0.004910586489529578 1109 9 -0.03438330675172807 1109 10-11 -0.19882376395534607 1109 12-13 0.17957057562319534 1109 14-15 1.2266227193858725 1109 16-17 1.1038022649864772 1109 18-19 0.8020798645798592 1109 20-21 1.581232254587519 1109 22-23 1.193604896565418 1109 24-25 -1.1335144387776026 1109 26-27 -1.6194688717715024 1109 28-29 -2.9257035727430427 1109 30-31 0.8707264592133015 1109 32-33 0.25916751377277336 1109 34-35 0.29551880867669666 1109 36-37 0.9122820162293834 1109 38-39 0.45080336330336834 1109 40-41 -1.2596056355924858 1109 42-43 1.6141866905024713 1109 44-45 0.4813681932103009 1109 46-47 0.7387477653924961 1109 48-49 -2.1705143175537813 1109 50-51 -2.4792135496082963 1109 52-53 -2.5792595197200505 1109 54-55 -0.7809762240683327 1109 56-57 -2.572399420754678 1109 58-59 -3.1475587643350806 1109 60-61 -1.9844086103954481 1109 62-63 0.3968180576733289 1109 64-65 1.2892314921920232 1109 66-67 1.5417013542013436 1109 68-69 -3.728164196914193 1109 70-71 -0.7597905018957611 1109 72-73 1.5238624314282205 1109 74-75 -0.7217507638560221 1109 76 3.511444257496887 1110 1 -1.2613276343539574 1110 2 -1.3940713526239819 1110 3 -0.04691843244474825 1110 4 -0.22400012268433755 1110 5 0.2978696741854634 1110 6 0.27955509402877254 1110 7 0.5052282512808759 1110 8 0.6932222748012222 1110 9 1.082499810131388 1110 10-11 -1.6046679197995033 1110 12-13 0.3094407054933299 1110 14-15 0.7136357063988612 1110 16-17 0.5388672000514134 1110 18-19 -1.3602577977578036 1110 20-21 0.4773361506914142 1110 22-23 -2.4167847138241925 1110 24-25 -2.4711767764399397 1110 26-27 2.432479180176543 1110 28-29 1.730140583101111 1110 30-31 -1.8890138005269606 1110 32-33 2.0481285527338216 1110 34-35 -2.6622733991155094 1110 36-37 1.7109833149306795 1110 38-39 2.2040501165501176 1110 40-41 -4.016099142085995 1110 42-43 -0.6260730497572666 1110 44-45 0.6924071542492527 1110 46-47 1.5634230900678219 1110 48-49 1.6931220460825784 1110 50-51 -2.641551211945959 1110 52-53 -0.7448439353044662 1110 54-55 -1.8556515487436513 1110 56-57 1.3204577221024678 1110 58-59 -1.939766556542871 1110 60-61 -0.7116813376681748 1110 62-63 -1.005779344924079 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-3.6979412400465037 2116 76 -0.9409366948840656 2117 1 1.1023087292824059 2117 2 0.5150195564669247 2117 3 0.10892572339940898 2117 4 0.4123635136792956 2117 5 0.2978696741854634 2117 6 0.5912434057170799 2117 7 0.9597737058263291 2117 8 1.0049105864895296 2117 9 0.822759550391126 2117 10-11 1.1940333789017927 2117 12-13 0.39385628990891774 2117 14-15 -0.13052013775698157 2117 16-17 -2.8247691635849534 2117 18-19 -2.9836344211344255 2117 20-21 1.1526608260160884 2117 22-23 0.2650334679939874 2117 24-25 -4.490657295920464 2117 26-27 -5.048040300342933 2117 28-29 -0.8542750013144769 2117 30-31 2.3707264592133015 2117 32-33 1.5091675137727734 2117 34-35 2.0455188086766967 2117 36-37 -1.159146555199186 2117 38-39 -4.192053779553778 2117 40-41 -2.9381770641639164 2117 42-43 0.5427581190739019 2117 44-45 -2.768631806789699 2117 46-47 -0.761252234607511 2117 48-49 -0.24194288898235072 2117 50-51 -2.6934992638940116 2117 52-53 0.38502619456566833 2117 54-55 -1.7452619383540409 2117 56-57 0.7490291506738984 2117 58-59 -1.6475587643350806 2117 60-61 0.30130567531883656 2117 62-63 1.4682466291018983 2117 64-65 -1.067911364950838 2117 66-67 -1.5297272172272223 2117 68-69 1.3432643745143764 2117 70-71 -3.545504787610046 2117 72-73 0.41671957428536643 2117 74-75 0.9568206647154014 2117 76 3.297158543211175 2118 1 -2.0405484135747365 2118 2 -1.4849804435330753 2118 3 -4.462502848029164 2118 4 -2.7304936291778468 2118 5 -0.273558897243106 2118 6 -0.5516137371400589 2118 7 0.6740594201120444 2118 8 1.0049105864895296 2118 9 -1.4629547353231587 2118 10-11 -1.6631094782410614 2118 12-13 -3.249000852948228 2118 14-15 -0.5590915663284122 2118 16-17 -4.3247691635849534 2118 18-19 -1.2693487068487102 2118 20-21 -0.7044820311267657 2118 22-23 0.4078906108511262 2118 24-25 0.8664855612223974 2118 26-27 -2.048040300342933 2118 28-29 -3.211417858457331 2118 30-31 1.6564407449275862 2118 32-33 -3.062261057655796 2118 34-35 0.18837595153383546 2118 36-37 -2.659146555199193 2118 38-39 0.6650890775890801 2118 40-41 -6.438177064163916 2118 42-43 0.18561526193104783 2118 44-45 -5.62577466393256 2118 46-47 1.595890622535343 2118 48-49 1.1151999681605105 2118 50-51 -0.8363564067511504 2118 52-53 -4.9006880911486235 2118 54-55 -4.6738333669254715 2118 56-57 -0.9652565636118169 2118 58-59 -0.004701621477941842 2118 60-61 -0.6272657532525869 2118 62-63 -4.674610513755248 2118 64-65 -1.6393399363794074 2118 66-67 -2.601155788655795 2118 68-69 -2.0138784826284777 2118 70-71 -0.7597905018957611 2118 72-73 2.3452910028567935 2118 74-75 0.5996778075725473 2118 76 -0.13141288536025542 2119 1 1.1023087292824059 2119 2 0.5150195564669247 2119 3 0.6803542948279784 2119 4 0.4123635136792956 2119 5 0.2978696741854634 2119 6 -1.09923278475911 2119 7 0.45977370582632915 2119 8 0.4811010626800112 2119 9 -1.129621401989823 2119 10-11 -0.5440618591934481 2119 12-13 1.6200467660993851 2119 14-15 -1.1424248996617408 2119 16-17 -4.658102496918289 2119 18-19 -0.6860153735153744 2119 20-21 -2.87114869779343 2119 22-23 -0.8182998653393483 2119 24-25 0.8545807993176382 2119 26-27 1.1900549377523078 2119 28-29 -9.997132144171616 2119 30-31 -5.343559255072414 2119 32-33 -1.0503562957510368 2119 34-35 -4.942576429418537 2119 36-37 -3.1829560790087115 2119 38-39 -5.227768065268062 2119 40-41 -3.235796111782964 2119 42-43 -1.9215275952118134 2119 44-45 -1.6257746639325603 2119 46-47 0.2149382415829706 2119 48-49 0.9128190157795473 2119 50-51 1.6993578789631343 2119 52-53 1.1112166707561357 2119 54-55 1.9094999664078571 2119 56-57 -0.3462089445641965 2119 58-59 -1.8261301929065112 2119 60-61 -0.6510752770621195 2119 62-63 -0.4603247994695323 2119 64-65 -4.151244698284174 2119 66-67 -0.3511557886557952 2119 68-69 -4.109116577866576 2119 70-71 -1.9145524066576662 2119 72-73 -2.928518520952732 2119 74-75 -0.364607906713168 2119 76 -2.4409366948840656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 4.0 25 10.0 26 38.0 27 52.0 28 101.0 29 133.0 30 221.0 31 283.0 32 447.0 33 587.0 34 763.0 35 748.0 36 224.0 37 101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 3.9859951521680577 19.822246162133045 11.338540263937517 64.85321842176138 2 1.6159439806086722 3.959062752491247 83.32884460005387 11.096148666846215 3 2.558577969297064 20.657150552114192 68.758416374899 8.02585510368974 4 1.5890115809318612 57.17748451387018 20.495556154053325 20.737947751144628 5 1.9391327767304067 31.672502019929976 61.836789657958526 4.551575545381093 6 3.3665499596014 63.722057635335304 9.749528683005655 23.161863722057635 7 3.501211957985457 31.26851602477781 8.187449501750606 57.04282251548612 8 2.2084567734985185 64.44923242660921 19.41826016698088 13.924050632911392 9 3.204955561540533 13.89711823323458 4.01292755184487 78.88499865338001 10-11 2.814435766226771 35.60463237274441 40.23700511715594 21.343926743872878 12-13 10.772959870724481 6.61190412065715 37.71882574737409 44.896310261244274 14-15 14.031780231618637 11.984917856180987 8.96848909237813 65.01481281982224 16-17 35.72582817129006 31.861028817667652 2.666307568004309 29.746835443037973 18-19 35.17371397791543 18.354430379746837 3.151090762186911 43.32076488015082 20-21 13.937516832749797 7.056288715324535 3.3126851602477783 75.69350929167788 22-23 13.775922434688932 41.852949097764615 3.2184217613789388 41.15270670616752 24-25 35.88742256935093 43.29383248047401 3.272286560732561 17.5464583894425 26-27 35.5776999730676 54.820899542149206 3.433880958793428 6.167519525989765 28-29 13.573929437112847 40.18314031780232 13.116078642607057 33.12685160247778 30-31 14.785887422569353 39.40210072717479 39.388634527336386 6.423377322919473 32-33 68.48909237813089 15.230272017236734 12.819822246162133 3.4608133584702396 34-35 15.028279019660651 40.33126851602478 41.36816590358201 3.272286560732561 36-37 32.776730406679235 37.866953945596556 17.034742795583085 12.321572852141125 38-39 13.81632103420415 12.173444653918663 33.59816859682198 40.41206571505521 40-41 6.275249124697011 11.890654457312147 44.51925666576892 37.31483975222192 42-43 39.64449232426609 5.171020737947751 43.9806086722327 11.20387826555346 44-45 8.133584702396982 31.955292216536495 39.02504713169944 20.88607594936709 46-47 40.65445731214651 5.440344734715863 19.4721249663345 34.433072986803126 48-49 33.04605440344734 3.4608133584702396 15.18987341772152 48.303258820360895 50-51 8.766496094802047 3.5954753568542954 45.219499057366015 42.41852949097765 52-53 9.453272286560733 4.578507945057905 72.82520872609749 13.143011042283867 54-55 4.5650417452194985 4.201454349582548 49.40748720711015 41.8260166980878 56-57 39.361702127659576 4.578507945057905 21.330460544034473 34.72932938324805 58-59 7.554538109345543 21.518987341772153 60.3420414758955 10.584433072986803 60-61 31.793697818475625 51.99299757608403 11.97145165634258 4.241852949097765 62-63 4.68623754376515 87.66496094802046 2.5855103689738757 5.063291139240507 64-65 1.7910045785079451 90.49286291408565 2.100727174791274 5.615405332615135 66-67 2.2353891731753297 88.91731753299219 2.396983571236197 6.450309722596283 68-69 2.976030164287638 82.48047401023432 3.4608133584702396 11.08268246700781 70-71 3.258820360894156 75.35685429571774 5.440344734715863 15.94398060867223 72-73 4.470778346350659 59.96498788042015 10.018852679773769 25.545381093455426 74-75 5.884729329383248 58.13358470239698 10.113116078642607 25.86856988957716 76 5.709668731483975 59.30514408833827 9.183948289792621 25.801238890385132 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 0.0 22 4.0 23 4.0 24 0.0 25 0.0 26 0.0 27 0.5 28 2.5 29 4.0 30 2.0 31 1.5 32 5.5 33 8.0 34 8.5 35 10.0 36 15.0 37 19.0 38 68.5 39 318.5 40 697.5 41 843.5 42 811.0 43 682.5 44 431.0 45 231.0 46 154.0 47 118.0 48 67.5 49 44.0 50 35.0 51 29.5 52 18.5 53 10.5 54 8.0 55 10.0 56 11.0 57 13.0 58 16.0 59 10.5 60 8.0 61 8.5 62 6.0 63 9.5 64 11.5 65 5.5 66 2.5 67 4.0 68 3.0 69 2.0 70 1.5 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 3713.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.67357931591705 #Duplication Level Percentage of deduplicated Percentage of total 1 76.56427758816837 18.12550498249394 2 12.059158134243459 5.709668731483975 3 3.6405005688282137 2.5855103689738757 4 1.2514220705346986 1.185025585779693 5 1.3651877133105803 1.6159439806086722 6 1.023890784982935 1.454349582547805 7 0.3412969283276451 0.5655803932130353 8 0.5688282138794084 1.0772959870724481 9 0.5688282138794084 1.2119579854565041 >10 1.8202502844141069 7.998922704012927 >50 0.4550625711035267 7.352545111769458 >100 0.22753128555176336 15.64772421222731 >500 0.0 0.0 >1k 0.11376564277588168 35.46997037436036 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 1317 35.46997037436036 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 432 11.63479666038244 RNA PCR Primer, Index 47 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 149 4.01292755184487 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 98 2.639375168327498 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT 65 1.7506059789927284 No Hit CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 59 1.5890115809318612 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT 51 1.3735523835173715 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT 38 1.0234311877188258 No Hit TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT 36 0.9695663883652034 No Hit CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 35 0.9426339886883921 No Hit CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT 24 0.6463775922434689 No Hit CTTATACACATCTCCGAGCCCACGAGACACTGGTAGATCTCGTATGCCGT 23 0.6194451925666576 No Hit TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 16 0.43091839482897926 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGTCTT 15 0.40398599515216804 No Hit CTTATACACATCTCCGAGCCCACGCGACAATGGTAGATCTCGTATGCCGT 14 0.37705359547535683 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGTCTT 14 0.37705359547535683 No Hit ACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCT 14 0.37705359547535683 RNA PCR Primer, Index 47 (95% over 23bp) TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 14 0.37705359547535683 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGTCTT 12 0.32318879612173446 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCC 11 0.29625639644492324 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCC 11 0.29625639644492324 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTCTGCCGTCTT 10 0.26932399676811203 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATTTCGTATGCCGT 10 0.26932399676811203 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCG 9 0.24239159709130084 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCC 9 0.24239159709130084 No Hit ATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCTT 9 0.24239159709130084 RNA PCR Primer, Index 23 (96% over 27bp) CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT 9 0.24239159709130084 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 9 0.24239159709130084 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCACGTATGCCGT 8 0.21545919741448963 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 8 0.21545919741448963 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCCCGTATGCCGT 8 0.21545919741448963 No Hit CTTATAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 8 0.21545919741448963 No Hit CTTATACACATCTCCGAGCACACGAGACAATGGTAGATCTCGTATGCCGT 8 0.21545919741448963 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT 7 0.18852679773767841 No Hit CTTATACACATATCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 7 0.18852679773767841 No Hit CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 7 0.18852679773767841 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGCTCTCGTATGCCGT 6 0.16159439806086723 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAAATCGTATGCCGT 6 0.16159439806086723 No Hit CTTATACACATCTCCGAGCCAACGAGACAATGGTAGATCTCGTATGCCGT 6 0.16159439806086723 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTAGTATGCCGT 6 0.16159439806086723 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTCGATCTCGTATGCCGT 6 0.16159439806086723 No Hit CTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTGCTTGA 6 0.16159439806086723 RNA PCR Primer, Index 47 (96% over 28bp) TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCG 6 0.16159439806086723 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACATCGTATGCCGT 6 0.16159439806086723 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG 6 0.16159439806086723 No Hit GTTCTGAGTGAGAAGTCATATCTTAGATCAGACTATGGCTGTCTCTTATA 5 0.13466199838405601 No Hit GTGGAGAAAAGAACTATTTCTTATTAATAAGTGACATCTAGGCACTGTCT 5 0.13466199838405601 No Hit CTTATACACATCTCCGAGACCACGAGACAATGGTAGATCTCGTATGCCGT 5 0.13466199838405601 No Hit GTTAAATATTATATGTCTAAGTACTGAGAAAATGAAAATGACATAAAAAC 5 0.13466199838405601 No Hit CTTATAAACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 5 0.13466199838405601 No Hit CTTAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC 5 0.13466199838405601 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTCTGCCGT 5 0.13466199838405601 No Hit ATACACATCTACGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 5 0.13466199838405601 RNA PCR Primer, Index 23 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCG 5 0.13466199838405601 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT 5 0.13466199838405601 No Hit GCAGCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCAC 5 0.13466199838405601 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGC 5 0.13466199838405601 No Hit TATTGTGACTGGTCATTTCAACATTTTCAATTTTACCCACTCTCACTTGC 4 0.10772959870724481 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAAATCTCGTATGCCGT 4 0.10772959870724481 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGGTAGATCTCGTATGCCGT 4 0.10772959870724481 No Hit CTTATACACATCTCCGAGCCCACGAGAAAATGGTAGATATCGTATGCCGT 4 0.10772959870724481 No Hit CTACCAGGATTAATCACATGAACAGATCCAATCTGTTCATATTCTGTCTC 4 0.10772959870724481 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCCCGTATGCCGTCTT 4 0.10772959870724481 No Hit CTCTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 4 0.10772959870724481 No Hit CTCTTATACACATCTCCGAGCCCAAGAGACAATGGTAGATCTCGTATGCC 4 0.10772959870724481 No Hit TCTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 4 0.10772959870724481 No Hit CTTATACACATCTCCGAGCCCACGAGACCATGGTAGAACTCGTATGCCGT 4 0.10772959870724481 No Hit ATACACATCTCCGAGCCCACGAGACACTGGTAGATCTCGTATGCCGTCTT 4 0.10772959870724481 RNA PCR Primer, Index 23 (95% over 21bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.05386479935362241 0.0 2 0.0 0.0 0.0 0.05386479935362241 0.0 3 0.0 0.0 0.0 0.10772959870724481 0.0 4 0.0 0.0 0.0 0.10772959870724481 0.0 5 0.0 0.0 0.0 0.13466199838405601 0.0 6 0.0 0.0 0.0 0.13466199838405601 0.0 7 0.0 0.0 0.0 0.13466199838405601 0.0 8 0.0 0.0 0.0 0.13466199838405601 0.0 9 0.0 0.0 0.0 0.13466199838405601 0.0 10 0.0 0.0 0.0 0.18852679773767841 0.0 11 0.0 0.0 0.0 0.18852679773767841 0.0 12 0.0 0.0 0.0 0.21545919741448963 0.0 13 0.0 0.0 0.0 0.21545919741448963 0.0 14 0.0 0.0 0.0 0.21545919741448963 0.0 15 0.0 0.0 0.0 0.21545919741448963 0.0 16 0.0 0.0 0.0 0.24239159709130084 0.0 17 0.0 0.0 0.0 0.24239159709130084 0.0 18 0.0 0.0 0.0 0.45785079450579047 0.0 19 0.0 0.0 0.0 0.5925127928898465 0.0 20 0.0 0.0 0.0 0.6463775922434689 0.0 21 0.0 0.0 0.0 0.6463775922434689 0.0 22 0.0 0.0 0.0 0.6463775922434689 0.0 23 0.0 0.0 0.0 0.7271747912739025 0.0 24 0.0 0.0 0.0 0.7541071909507137 0.0 25 0.0 0.0 0.0 0.7541071909507137 0.0 26 0.0 0.0 0.0 0.7541071909507137 0.0 27 0.0 0.0 0.0 0.7810395906275249 0.0 28 0.0 0.0 0.0 0.7810395906275249 0.0 29 0.0 0.0 0.0 0.8079719903043361 0.0 30 0.0 0.0 0.0 0.8079719903043361 0.0 31 0.0 0.0 0.0 0.8079719903043361 0.0 32 0.0 0.0 0.0 0.8618367896579585 0.0 33 0.0 0.0 0.0 0.9157015890115809 0.0 34 0.0 0.0 0.0 0.9426339886883921 0.0 35 0.0 0.0 0.0 1.1311607864260707 0.0 36 0.0 0.0 0.0 1.4274171828709938 0.0 37 0.0 0.0 0.0 1.723673579315917 0.0 38 0.0 0.0 0.0 2.1545919741448962 0.0 39 0.0 0.0 0.0 2.774037166711554 0.0 40 0.0 0.0 0.0 3.204955561540533 0.0 41 0.0 0.0 0.0 3.3126851602477783 0.0 42 0.0 0.0 0.0 3.5281443576622675 0.0 43 0.0 0.0 0.0 3.7705359547535684 0.0 44 0.0 0.0 0.0 4.01292755184487 0.0 45 0.0 0.0 0.0 4.578507945057905 0.0 46 0.0 0.0 0.0 4.8478319418260165 0.0 47 0.0 0.0 0.0 4.982493940210072 0.0 48 0.0 0.0 0.0 5.278750336654996 0.0 49 0.0 0.0 0.0 5.467277134392674 0.0 50 0.0 0.0 0.0 5.709668731483975 0.0 51 0.0 0.0 0.0 5.844330729868031 0.0 52 0.0 0.0 0.0 5.925127928898465 0.0 53 0.0 0.0 0.0 5.978992728252087 0.0 54 0.0 0.0 0.0 6.167519525989766 0.0 55 0.0 0.0 0.0 6.356046323727444 0.0 56 0.0 0.0 0.0 6.356046323727444 0.0 57 0.0 0.0 0.0 6.356046323727444 0.0 58 0.0 0.0 0.0 6.382978723404255 0.0 59 0.0 0.0 0.0 6.598437920818745 0.0 60 0.0 0.0 0.0 6.679235119849179 0.0 61 0.0 0.0 0.0 6.733099919202801 0.0 62 0.0 0.0 0.0 6.7869647185564235 0.0 63 0.0 0.0 0.0 6.867761917586857 0.0 64 0.0 0.0 0.0 6.867761917586857 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGATA 15 0.0020532461 70.00001 33 GATATCG 15 0.0020532461 70.00001 36 TATCGTA 15 0.0020532461 70.00001 38 ATATCGT 15 0.0020532461 70.00001 37 TAGATAT 15 0.0020532461 70.00001 34 AGATATC 15 0.0020532461 70.00001 35 ACCATGG 20 6.931956E-5 70.0 27 CCATGGT 20 6.931956E-5 70.0 28 CATGGTA 20 6.931956E-5 70.0 29 CGAGACC 20 6.931956E-5 70.0 23 ATCGTAT 20 6.931956E-5 70.0 39 AGACCAT 20 6.931956E-5 70.0 25 GACCATG 20 6.931956E-5 70.0 26 GAGACCA 20 6.931956E-5 70.0 24 AAACCAA 25 2.0921287E-4 56.000004 68 TTATACA 255 0.0 53.52941 2 CTTATAC 255 0.0 53.52941 1 GGTAGAA 20 0.006419566 52.5 32 AAAACCA 40 5.6440695E-7 52.5 67 TATACAC 270 0.0 49.25926 3 >>END_MODULE