FastQCFastQC Report
Wed 25 May 2016
SRR1780661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41630
Sequences flagged as poor quality0
Sequence length50
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA760.18256065337497No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATC700.16814797021378813No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG670.1609416286331972No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG580.13932260389142445No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC440.10569300984866682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG357.1653376E-637.6813161
TAAGCGG301.2862596E-436.6346171
AGCCACG505.6972567E-835.2115527
ATCGGGC250.002323082435.2115527
CGTCAGA250.00233686235.1692282
TTTACAC551.3305544E-731.9720292
CTTTACA551.3305544E-731.9720291
CAATGTG353.1814398E-431.40109832
CGGGTCG300.00563974229.37831312
GATCGGG300.00567310829.34296226
GAGCCAC453.967979E-529.34296226
CCGACGC453.967979E-529.34296218
GCCACGG300.00567310829.34296228
GAGATCT300.00567310829.34296239
GACGAAA454.000788E-529.30769323
GTCAGAT300.005706629729.3076923
TCAGATG300.005706629729.3076924
GCGTCAG300.005706629729.3076921
AGAACGG406.904481E-427.50902427
AATGTGT406.904481E-427.50902433