##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1780661_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41630
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.726927696372808	16.0	10.0	30.0	7.0	33.0
2	20.073937064616864	18.0	10.0	30.0	7.0	33.0
3	20.26452077828489	22.0	10.0	30.0	7.0	34.0
4	22.467042997838096	23.0	10.0	33.0	7.0	37.0
5	22.179365841940907	22.0	10.0	33.0	7.0	37.0
6	22.504155656978142	24.0	11.0	33.0	8.0	37.0
7	22.382104251741534	23.0	11.0	33.0	8.0	37.0
8	22.184794619264952	23.0	10.0	33.0	7.0	37.0
9	22.797381695892387	24.0	10.0	34.0	7.0	39.0
10	22.491424453519098	23.0	10.0	34.0	7.0	39.0
11	22.81061734326207	24.0	10.0	35.0	7.0	39.0
12	22.63026663463848	23.0	10.0	34.0	7.0	39.0
13	22.71085755464809	23.0	10.0	35.0	7.0	39.0
14	23.02536632236368	23.0	10.0	35.0	7.0	40.0
15	22.67391304347826	22.0	10.0	34.0	7.0	40.0
16	22.65378332932981	22.0	10.0	34.0	7.0	40.0
17	22.75207782848907	23.0	10.0	34.0	7.0	40.0
18	22.384674513571944	22.0	10.0	34.0	7.0	40.0
19	22.36622627912563	21.0	10.0	34.0	7.0	40.0
20	22.476531347585876	22.0	10.0	34.0	7.0	40.0
21	22.208959884698533	21.0	9.0	34.0	7.0	40.0
22	22.211361998558733	21.0	9.0	34.0	7.0	40.0
23	22.16319961566178	21.0	9.0	34.0	7.0	40.0
24	21.665169349027146	19.0	8.0	34.0	7.0	40.0
25	21.607734806629836	19.0	8.0	33.0	7.0	40.0
26	21.33036271919289	18.0	8.0	33.0	7.0	40.0
27	21.074681719913524	18.0	8.0	32.0	7.0	40.0
28	20.976507326447273	18.0	8.0	32.0	7.0	40.0
29	20.765505644967572	17.0	8.0	32.0	7.0	40.0
30	20.544222916166227	17.0	8.0	32.0	7.0	39.0
31	20.46927696372808	17.0	8.0	32.0	7.0	39.0
32	20.196661061734325	16.0	8.0	31.0	7.0	39.0
33	20.235335094883496	16.0	8.0	32.0	7.0	39.0
34	19.936512130674995	16.0	8.0	31.0	7.0	39.0
35	19.74210905596925	16.0	8.0	31.0	7.0	39.0
36	19.68023060293058	15.0	8.0	31.0	7.0	39.0
37	19.525342301225077	15.0	8.0	30.0	7.0	39.0
38	19.560052846504924	15.0	8.0	30.0	7.0	39.0
39	19.21011289935143	15.0	8.0	30.0	7.0	39.0
40	18.817487388902233	14.0	8.0	30.0	7.0	38.0
41	18.870646168628394	15.0	8.0	30.0	7.0	38.0
42	18.861542157098246	14.0	8.0	30.0	7.0	38.0
43	18.935551285130916	14.0	8.0	30.0	7.0	38.0
44	18.90900792697574	14.0	8.0	30.0	7.0	38.0
45	18.989791016094163	15.0	8.0	30.0	7.0	38.0
46	18.74412683161182	14.0	8.0	29.0	7.0	38.0
47	18.71988950276243	14.0	8.0	29.0	7.0	37.0
48	18.656521739130437	14.0	8.0	29.0	7.0	37.0
49	18.41035311073745	13.0	8.0	28.0	7.0	37.0
50	18.357770838337736	13.0	8.0	28.0	7.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.330214496945871
1101	2	0.8924367225048755
1101	3	-0.7752659024158497
1101	4	-0.2613600666964153
1101	5	1.0188809427319327
1101	6	0.9831153043668373
1101	7	0.8990418569645584
1101	8	0.8806357904359245
1101	9	0.8122001591920522
1101	10	0.8187819619975656
1101	11	1.5112425578114035
1101	12	-0.3587183600107302
1101	13	0.6032206359964967
1101	14	0.0368685348115676
1101	15	2.168154818006478
1101	16	1.5962187445636928
1101	17	1.2654811331842915
1101	18	1.1288943130980833
1101	19	0.2183765105108506
1101	20	-0.07818399377790186
1101	21	-0.2227112574204817
1101	22	0.329514794294262
1101	23	0.9035462139448285
1101	24	0.4457438993621139
1101	25	1.0559673393846012
1101	26	-0.07193273043824178
1101	27	-0.49659677656569556
1101	28	0.8812505853401333
1101	29	0.4845849492780019
1101	30	0.01635097468298241
1101	31	0.36919687849595917
1101	32	0.21185233575889484
1101	33	-0.3140210854729375
1101	34	0.19785852682536031
1101	35	1.1370988019446706
1101	36	1.2631737323307846
1101	37	1.6814506381705243
1101	38	-1.0611423145739494
1101	39	-0.6612646640448752
1101	40	0.2942873495788767
1101	41	0.7305959237778232
1101	42	-0.027533772322879457
1101	43	0.44098788691808366
1101	44	-0.29287856056072314
1101	45	2.211003996226381
1101	46	0.5615027454519286
1101	47	1.1029173975966202
1101	48	1.8818196669545841
1101	49	0.6590282549266107
1101	50	1.4944491460301066
1102	1	-0.9996117814979755
1102	2	-0.944852242506137
1102	3	-2.3074410920414934
1102	4	-1.265519596914178
1102	5	-1.2720414942634193
1102	6	-4.187914791057679
1102	7	-1.409497413894261
1102	8	-1.458243583187759
1102	9	-3.0334078662544854
1102	10	-2.704829488944444
1102	11	-0.8616470922987034
1102	12	-1.6481727275174585
1102	13	-1.2010367655205094
1102	14	-1.0720132855946005
1102	15	-1.6365919400067348
1102	16	-2.616896009534667
1102	17	-1.3359380006547106
1102	18	-1.903158537403506
1102	19	0.043186884868571696
1102	20	-1.5822211848716137
1102	21	-1.6198240540928595
1102	22	-2.3680628910495116
1102	23	-2.29048363680144
1102	24	-1.5378626580149337
1102	25	-1.5669834802875293
1102	26	-2.504523873085663
1102	27	-1.1344729693721547
1102	28	-0.4759796568913295
1102	29	-1.2210671182531954
1102	30	-1.6753544327062997
1102	31	-1.3641038310709597
1102	32	-1.8784339377913852
1102	33	-0.5919755753187879
1102	34	-0.11557430899553367
1102	35	-1.7782425242114357
1102	36	-0.20562979103598877
1102	37	0.3413478733063222
1102	38	-0.7185927672287065
1102	39	-0.5543402696235411
1102	40	-1.9281251779474253
1102	41	-0.5522521310301052
1102	42	-1.3235944525284111
1102	43	-0.5169274544080729
1102	44	0.7616846887664117
1102	45	-0.9543984994917487
1102	46	-0.7920573230579819
1102	47	-1.1243886948917563
1102	48	-0.6205047764024023
1102	49	-1.2842063915133188
1102	50	-1.192362696397101
1103	1	0.17535951586138054
1103	2	1.0452740491126917
1103	3	1.535957300610633
1103	4	1.1409097945898417
1103	5	0.6907598834633326
1103	6	0.5425100080238359
1103	7	-0.5488774368563725
1103	8	-0.5009531124644511
1103	9	0.4311156699108416
1103	10	-0.007699753008740373
1103	11	0.23961582388958647
1103	12	-0.024292130502534093
1103	13	-0.1597049629946774
1103	14	0.07999589925040596
1103	15	1.7023288407050892
1103	16	0.5523347596961017
1103	17	-0.029853040838407452
1103	18	1.3735349183944265
1103	19	0.36465646006949015
1103	20	-0.4194198071448625
1103	21	-1.2340605638517559
1103	22	-0.15497448691633053
1103	23	-0.4964537860551701
1103	24	-0.5840165041687797
1103	25	-0.6131373264413753
1103	26	-0.2358671566677515
1103	27	0.6650677883775593
1103	28	-0.17448711957789698
1103	29	0.2714701951796421
1103	30	0.6589739255026537
1103	31	0.7114415191012569
1103	32	-0.8194213086295044
1103	33	1.4539486497099148
1103	34	-0.3559876270207951
1103	35	0.20040271115021824
1103	36	-1.2846194580853592
1103	37	-0.3881584412746193
1103	38	-0.7078005743469618
1103	39	0.1916987659677325
1103	40	0.49529617681721305
1103	41	-0.3150535087568578
1103	42	-0.31303188077180977
1103	43	-0.555733424557328
1103	44	-0.9271786866641278
1103	45	-1.046246949549154
1103	46	0.6621332624758871
1103	47	-0.2635390967287279
1103	48	-0.4992648223266265
1103	49	-0.11222016878082997
1103	50	0.2429989568750024
1104	1	-0.25760751710565444
1104	2	-0.4890360851974407
1104	3	-0.7258253611720313
1104	4	-1.490347836667791
1104	5	-1.2059434132399645
1104	6	-1.5378318723180442
1104	7	-0.935202223181161
1104	8	-0.16542197693331673
1104	9	-1.5351846963895248
1104	10	-0.9171014624104501
1104	11	-0.9066601223863593
1104	12	-0.6465143527247541
1104	13	-0.3855901888799025
1104	14	-0.361518142263634
1104	15	-0.857866519490269
1104	16	-0.41054680318546133
1104	17	-0.9106833216686887
1104	18	-1.8877593428998338
1104	19	-1.9918758964628687
1104	20	-1.3573929451180007
1104	21	-2.0618993916905843
1104	22	-1.4839122158540583
1104	23	-0.9853426749440608
1104	24	-0.6648467849990602
1104	25	-0.852697766001814
1104	26	-1.5850735058740995
1104	27	-1.8338333105235414
1104	28	-1.8697252148159933
1104	29	-0.28408536037591503
1104	30	0.966910433439164
1104	31	0.07749768515742161
1104	32	0.3861952969871041
1104	33	0.10596330172456447
1104	34	0.3565886855555185
1104	35	-0.21913330838580336
1104	36	1.20280422933833
1104	37	-0.40207785519403316
1104	38	-0.7756882422346294
1104	39	0.5565339308028996
1104	40	0.2154426969637342
1104	41	-0.28501687872022785
1104	42	-0.7621161298560573
1104	43	-0.2477969166208176
1104	44	-0.7867635462489879
1104	45	-0.429641332943536
1104	46	0.06579626613889289
1104	47	-0.4908895240791544
1104	48	0.24872052565872238
1104	49	0.10243184587118392
1104	50	-0.6590767452007
1105	1	-1.4398021340828784
1105	2	-0.5007683143209753
1105	3	-0.812091751786248
1105	4	-1.1846197483310057
1105	5	-0.3384485892234004
1105	6	-0.6151265583083827
1105	7	-1.930371305306764
1105	8	-0.4904737367676866
1105	9	-1.3985387336566042
1105	10	0.8703381511161226
1105	11	-0.7251556365340477
1105	12	0.6916498984829751
1105	13	-1.1820015070303498
1105	14	-1.3891233872601845
1105	15	0.3050044259893703
1105	16	0.6729590629291025
1105	17	-0.11703252801789432
1105	18	-0.8449712131551834
1105	19	-0.3610960482917136
1105	20	-0.28630943925188745
1105	21	0.9588045420568285
1105	22	0.6051146992598113
1105	23	0.6136911414810591
1105	24	1.331702559376371
1105	25	-0.6394472484034708
1105	26	-0.6706497653634038
1105	27	-2.6992577411653613
1105	28	-0.6295595833460155
1105	29	-1.670558790327604
1105	30	-1.2511710020335762
1105	31	-1.2468639435520323
1105	32	-2.0499675739583765
1105	33	-3.0845128887516253
1105	34	-2.7006921978123195
1105	35	-0.946531514044878
1105	36	-1.46455256845325
1105	37	-1.3482476274507604
1105	38	-1.8881796155955684
1105	39	-1.3295944335863332
1105	40	-1.2255510918561434
1105	41	-0.4423667752005578
1105	42	-1.7973128172266577
1105	43	-1.929646468035589
1105	44	-1.6473570590164144
1105	45	-0.9983093798724525
1105	46	-2.0604977297203213
1105	47	-0.7034499105525853
1105	48	-1.4883395157491464
1105	49	-0.9099905143772631
1105	50	-1.1821961378853096
1106	1	-1.2658890906046167
1106	2	-0.9065654157702525
1106	3	-1.2758598677282755
1106	4	-0.7643298932585445
1106	5	-0.7152601834262988
1106	6	-2.02092365975766
1106	7	0.05513594107004849
1106	8	-1.3020679396662374
1106	9	-0.8188285887290689
1106	10	-2.274589385115764
1106	11	-0.855590419142743
1106	12	-2.496755898618474
1106	13	-0.6167841157260021
1106	14	-3.099268314796415
1106	15	-1.8689086174888878
1106	16	-1.616896009534667
1106	17	-2.3199310787425347
1106	18	-1.2652610682276482
1106	19	-2.2741395265525846
1106	20	-1.5616717580924657
1106	21	-0.9542389362040424
1106	22	-1.4528563152329426
1106	23	0.25137230090135176
1106	24	-0.6393119333772539
1106	25	-1.7553892773889785
1106	26	-2.250359910290939
1106	27	-0.960127306382752
1106	28	-1.4411537862445662
1106	29	-1.3082399497478931
1106	30	-1.2366782484103886
1106	31	-0.44976249427666914
1106	32	-0.8615617768569273
1106	33	-0.345382453969016
1106	34	-1.2659095891166672
1106	35	-1.8740677459289365
1106	36	-2.1746974959894843
1106	37	-0.667088207160905
1106	38	-1.5403535286390486
1106	39	-0.749884288658798
1106	40	-1.6458409469286046
1106	41	-1.413381267954179
1106	42	-0.6523852809947748
1106	43	-1.5238493665863118
1106	44	-1.4009802474222113
1106	45	-1.3606282204521634
1106	46	-0.2923817876913368
1106	47	-0.39910208446562834
1106	48	-1.2419627041549433
1106	49	-1.9389760216236382
1106	50	-1.8778483117983562
1107	1	1.4676672081690718
1107	2	0.291427895266537
1107	3	0.22826499291832292
1107	4	1.3716790253590716
1107	5	1.5522983450017946
1107	6	1.6655869311007585
1107	7	2.0511225631436254
1107	8	3.729816118304779
1107	9	2.9695772083723817
1107	10	2.6538387085297224
1107	11	2.731923516197277
1107	12	3.4526309464205447
1107	13	3.5479873446976313
1107	14	1.664611283865792
1107	15	3.548482686858936
1107	16	1.290796298157641
1107	17	2.5701469591615904
1107	18	3.0196887645482704
1107	19	1.8877333831464114
1107	20	0.7959648082397521
1107	21	2.9813240515328587
1107	22	2.552717820775978
1107	23	3.3189308293294424
1107	24	0.6621373419850656
1107	25	2.0022472889432414
1107	26	1.7179789971784025
1107	27	2.5881447114544827
1107	28	2.440897495806716
1107	29	3.6560855797950254
1107	30	2.5512816178103463
1107	31	2.957595365255102
1107	32	2.319040229832037
1107	33	0.884717880479144
1107	34	1.3824739114407443
1107	35	2.7234796342271395
1107	36	2.1307651572992583
1107	37	1.857995404879226
1107	38	1.5691225025761142
1107	39	1.60708338135235
1107	40	1.3722192537402904
1107	41	1.531100337396989
1107	42	1.0869681192281924
1107	43	0.47503580621190267
1107	44	1.6728213133358736
1107	45	1.092214588912384
1107	46	0.8929024932451171
1107	47	-0.9250775582671871
1107	48	0.9315044084426027
1107	49	1.1185490619884
1107	50	2.366075879951925
1108	1	0.9753595158613813
1108	2	0.5375817414203858
1108	3	-0.8025042378509077
1108	4	-0.08985943617939185
1108	5	0.05999065269410053
1108	6	0.15789462340845262
1108	7	-0.3488774368563732
1108	8	1.1298161183047775
1108	9	1.1080387468339197
1108	10	1.9923002469912596
1108	11	0.6550004392742004
1108	12	-1.024292130502534
1108	13	1.02491042162071
1108	14	1.6492266684811767
1108	15	0.17925191762816794
1108	16	0.4600270673884097
1108	17	0.2009161899308225
1108	18	1.6504579953175025
1108	19	0.4877333831464128
1108	20	0.9036571159320594
1108	21	-0.95713748692868
1108	22	-0.5549744869163291
1108	23	-0.8041460937474803
1108	24	-0.8917088118610863
1108	25	-0.8746757879798359
1108	26	-1.7743286951292916
1108	27	-1.7195475962378275
1108	28	-1.7437178888086677
1108	29	-0.9131451894357419
1108	30	0.3820508485795777
1108	31	0.11144151910125544
1108	32	-0.8809597701679657
1108	33	-0.6998975041362421
1108	34	-0.10983378086694628
1108	35	-1.3995972888497832
1108	36	-0.3615425350084358
1108	37	-1.280466133582312
1108	38	0.07681481026842363
1108	39	-0.715993541724572
1108	40	0.12606540758644513
1108	41	-0.02274581644916296
1108	42	-0.651493419233347
1108	43	-0.1249641937880952
1108	44	-1.1733325328179731
1108	45	-1.7385546418568474
1108	46	-1.5994051990625735
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2210	47	-0.9240345334953481
2210	48	0.04936620766033428
2210	49	-1.6957925286243736
2210	50	-1.4433113429946225
2211	1	2.6263210543229185
2211	2	2.422918279881923
2211	3	3.0633611467644783
2211	4	4.556775179205225
2211	5	4.561913729617178
2211	6	4.2295292387930665
2211	7	2.589103332374396
2211	8	4.351691118304778
2211	9	3.4477022083723803
2211	10	2.825954093145107
2211	11	3.0973081315818938
2211	12	3.7632078694974673
2211	13	2.4556796523899393
2211	14	2.815813206942714
2211	15	3.1141076868589366
2211	16	2.304978990465333
2211	17	2.966300805315438
2211	18	2.9324291491636565
2211	19	1.89230069083872
2211	20	4.5798590390089835
2211	21	4.295987513071321
2211	22	4.6128139746221315
2211	23	1.8566712139448285
2211	24	2.9308873419850663
2211	25	4.651766519712471
2211	26	2.3578828433322485
2211	27	2.552327403762174
2211	28	2.1942628804221016
2211	29	2.146470195179642
2211	30	2.0058489255026544
2211	31	2.836922288332026
2211	32	2.7505306144474204
2211	33	1.4311121112483747
2211	34	2.3228585268253603
2211	35	1.4314123265348329
2211	36	0.9552843880684883
2211	37	1.4253030971869194
2211	38	1.8525359641145762
2211	39	3.090016073660042
2211	40	2.826305792201829
2211	41	0.7544176450892977
2211	42	1.2393719653820376
2211	43	0.5098915754426727
2211	44	-0.5065056097410512
2211	45	1.6088011273739227
2211	46	0.3217486470912725
2211	47	-0.023875635190265143
2211	48	0.7516967161349122
2211	49	1.0481163696807094
2211	50	2.2208835722596163
2212	1	0.6332654987673614
2212	2	1.0149321687708124
2212	3	1.184888924542257
2212	4	0.21996962364966777
2212	5	0.7563581740616208
2212	6	0.6010570165708451
2212	7	1.1481311101521747
2212	8	1.9159272294158889
2212	9	1.4945772083723803
2212	10	2.1245652042562178
2212	11	0.7223081315818938
2212	12	1.7701523139419102
2212	13	0.6779018746121608
2212	14	0.8210215402760461
2212	15	1.3762604646367151
2212	16	2.299770657132001
2212	17	2.216300805315438
2212	18	1.7709708158303243
2212	19	1.4982034686164987
2212	20	0.4878451501200942
2212	21	1.4956402908490993
2212	22	0.9426750857332422
2212	23	2.1118795472781606
2212	24	1.4343595642072877
2212	25	2.1274609641569135
2212	26	2.291910621110027
2212	27	1.936007959317731
2212	28	1.4616239915332123
2212	29	0.6464701951796421
2212	30	2.6673072588359865
2212	31	1.9584500661098048
2212	32	2.2453222811140883
2212	33	1.8738204445817068
2212	34	3.0867474157142496
2212	35	2.5268984376459436
2212	36	1.8059788325129311
2212	37	1.3697475416313623
2212	38	1.6528831863367976
2212	39	0.7410577403267098
2212	40	1.560680792201829
2212	41	0.8637926450892977
2212	42	1.430344187604259
2212	43	2.452599908776005
2212	44	1.705299945814506
2212	45	1.5723427940405905
2212	46	1.7661930915357154
2212	47	2.3146660314764027
2212	48	2.3697522716904693
2212	49	1.4179080363473773
2212	50	1.2833835722596163
2213	1	-0.2018039456770815
2213	2	0.6416682798819231
2213	3	-0.10851385323552165
2213	4	-1.068224820794775
2213	5	0.09316372961717789
2213	6	-0.2704707612069335
2213	7	-0.19214666762560384
2213	8	0.8516911183047782
2213	9	0.7758272083723803
2213	10	0.07595409314510704
2213	11	0.08168313158189378
2213	12	-0.5492921305025327
2213	13	0.5338046523899393
2213	14	0.503313206942714
2213	15	-0.3390173131410634
2213	16	0.039353990465333055
2213	17	-1.0336991946845622
2213	18	0.1511791491636565
2213	19	0.5173006908387201
2213	20	0.6111090390089835
2213	21	0.8897375130713208
2213	22	0.050313974622131497
2213	23	0.20042121394482848
2213	24	0.6183873419850663
2213	25	1.5580165197124707
2213	26	0.2485078433322485
2213	27	0.09920240376217393
2213	28	-1.0244871195778984
2213	29	-1.353529804820358
2213	30	-1.9785260744973456
2213	31	1.0400472883320262
2213	32	0.9380306144474204
2213	33	-0.4126378887516253
2213	34	0.3853585268253603
2213	35	-0.13108767346516714
2213	36	-0.37284061193151175
2213	37	0.3940530971869194
2213	38	0.9931609641145762
2213	39	-0.7068589263399581
2213	40	0.8575557922018291
2213	41	0.06691764508929765
2213	42	0.5362469653820376
2213	43	1.9005165754426727
2213	44	1.0716193902589488
2213	45	0.21817612737392267
2213	46	-0.11575135290872751
2213	47	0.7886243648097349
2213	48	2.329821716134912
2213	49	0.18874136968070943
2213	50	-0.0603664277403837
2214	1	-0.34289536358753026
2214	2	-0.1239567201180769
2214	3	-0.7701276592056701
2214	4	-0.2505942237798493
2214	5	-0.7795041808305818
2214	6	-1.8595565821024564
2214	7	-0.5587511452375438
2214	8	-0.9806316428892501
2214	9	0.0561443725514863
2214	10	-1.794381727750416
2214	11	0.48163648979084783
2214	12	0.7100362277064214
2214	13	-0.6338725864160324
2214	14	-0.012311793057286025
2214	15	-0.21868149224554045
2214	16	-1.2139109349078012
2214	17	-1.574743970803965
2214	18	-0.9628600299408205
2214	19	-2.180693712146354
2214	20	-0.9852062594984794
2214	21	-1.2765404720033047
2214	22	-1.3680628910495116
2214	23	-0.39496124874174043
2214	24	-1.9408477326417994
2214	25	-0.8057894504367837
2214	26	-1.176165664130437
2214	27	-0.02999535743185433
2214	28	1.6135725819146387
2214	29	-0.2658432376561777
2214	30	0.38434705983101125
2214	31	-0.12529786092170525
2214	32	-1.6396279676421308
2214	33	-1.5919755753187879
2214	34	-2.0260220701895655
2214	35	-0.8827201361517361
2214	36	-1.7727939701404658
2214	37	-0.6586521266936778
2214	38	-1.3603838120048266
2214	39	-0.6886686278324952
2214	40	-0.6594684615295137
2214	41	0.05968816747735772
2214	42	-1.0250869898418422
2214	43	0.48307254559192714
2214	44	0.06019215145297707
2214	45	-0.04395073829772045
2214	46	-0.07563941261021867
2214	47	0.02486503645152638
2214	48	-0.6652808958053846
2214	49	-0.4782362422595874
2214	50	-0.9237059799791894
2215	1	-0.08617894567708007
2215	2	-0.30857210473346086
2215	3	-1.7409657763124464
2215	4	-0.8129363592563124
2215	5	-0.03231703961359145
2215	6	0.2040484695622986
2215	7	0.2357379477590129
2215	8	-0.2086454201567598
2215	9	-1.7688843300891577
2215	10	-0.946161291470279
2215	11	-0.6219226376488756
2215	12	-1.0550613612717648
2215	13	-2.0058588091485205
2215	14	-1.2584656392111313
2215	15	-1.913055774679524
2215	16	-2.1861267787654377
2215	17	-2.491391502376871
2215	18	-1.3341573892978822
2215	19	-1.8353435399305091
2215	20	-1.2809582686833245
2215	21	-2.5879067176979085
2215	22	-1.8626667946086393
2215	23	-0.6656845552859423
2215	24	-1.3070934272457038
2215	25	-2.4439065572106067
2215	26	-1.9127902335908296
2215	27	-2.53493221162244
2215	28	-0.7437178888086677
2215	29	-0.5439144202049739
2215	30	-2.0025645360358055
2215	31	-3.4577892501295118
2215	32	-3.127113616321811
2215	33	-0.7460513502900881
2215	34	-0.14060301163617694
2215	35	-0.12267421192670724
2215	36	-1.7769271503930497
2215	37	-0.95738921050539
2215	38	-0.8154928820392691
2215	39	-0.08522431095534344
2215	40	-0.10470382318278482
2215	41	0.13110033739699034
2215	42	-0.743801111541039
2215	43	-1.278810347634252
2215	44	-0.5733325328179752
2215	45	-0.86163156493377
2215	46	-2.0147898144471874
2215	47	-3.155846789036419
2215	48	-2.960803283865088
2215	49	-3.6506817072423683
2215	50	-3.1570010431249997
2216	1	2.374427114928981
2216	2	2.133619037457681
2216	3	2.176050540703873
2216	4	1.9800706337506782
2216	5	2.67933797204142
2216	6	1.9975216630354922
2216	7	2.332474544495607
2216	8	1.7479979364865947
2216	9	1.3241226629178335
2216	10	2.638454093145107
2216	11	2.6048838891576516
2216	12	1.8635866573762563
2216	13	2.713255409965697
2216	14	0.9119306311851396
2216	15	2.6363614747377255
2216	16	1.3982555056168486
2216	17	1.2769068659215002
2216	18	-0.21387766901816008
2216	19	1.383304478717509
2216	20	-0.31265990038495417
2216	21	1.0246806948895042
2216	22	1.024745792803948
2216	23	0.07021288061149633
2216	24	1.3560767359244608
2216	25	2.2208953075912596
2216	26	2.2944358736352797
2216	27	2.1834827067924785
2216	28	1.1952098501190704
2216	29	0.7411671648766109
2216	30	1.88321634974508
2216	31	1.9230965307562684
2216	32	1.8551707659625727
2216	33	1.188214383975648
2216	34	2.0614948904617236
2216	35	0.3703327810802861
2216	36	1.785776812310914
2216	37	0.9593940062778294
2216	38	1.7942973277509395
2216	39	1.699391073660042
2216	40	0.26522624674728235
2216	41	1.0418229481196022
2216	42	-2.1136795129450547E-4
2216	43	0.8351756663517627
2216	44	0.5133807538953121
2216	45	-0.21174811505031954
2216	46	0.5376577380003624
2216	47	2.0343630011733715
2216	48	1.6816209585591544
2216	49	1.4141201575594984
2216	50	1.0523229661990108
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2.0
5	1.0
6	42.0
7	669.0
8	1962.0
9	2708.0
10	2286.0
11	1907.0
12	1832.0
13	1911.0
14	1887.0
15	1947.0
16	1670.0
17	1583.0
18	1464.0
19	1315.0
20	1254.0
21	1068.0
22	991.0
23	1005.0
24	912.0
25	872.0
26	840.0
27	816.0
28	750.0
29	712.0
30	651.0
31	663.0
32	596.0
33	606.0
34	614.0
35	644.0
36	793.0
37	1038.0
38	1114.0
39	2505.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.956035498689246	19.52668414343779	19.98124053007528	28.536039827797687
2	19.242780954211312	26.93989333589584	32.902512852544085	20.91481285734877
3	29.37855821662783	21.444185543731535	27.730668524346008	21.446587715294626
4	21.42205140523661	20.41316358395388	25.43838577948595	32.72639923132357
5	20.343502282008167	26.7475378332933	26.53134758587557	26.377612298822967
6	38.011049723756905	25.62575066058131	12.942589478741292	23.420610136920487
7	38.85659380254624	22.488589959164067	13.790535671390824	24.864280566898874
8	30.965649771799182	24.657698774921933	20.922411722315637	23.45423973096325
9	32.55104491952919	16.038914244535192	15.892385299063175	35.517655536872454
10	28.097525822723995	22.337256785971654	26.512130674993994	23.053086716310354
11	37.099137620408854	17.266809195512746	21.225587931490068	24.40846525258834
12	27.463367763631997	20.3987509007927	24.287773240451596	27.85010809512371
13	31.048813298741234	15.124435476121842	24.33218026328433	29.494570961852602
14	36.80809148568134	16.922929079377283	18.222660003843934	28.046319431097444
15	29.084182202575438	22.607149721314627	19.966846050355564	28.34182202575437
16	34.00871092716029	17.804461342252807	20.01588180089997	28.170945929686937
17	40.99884692995099	17.783703276640722	17.125492457000096	24.091957336408186
18	34.55328569022354	16.494622103515482	18.008133015712602	30.943959190548377
19	31.972658130355253	17.473765283527488	21.786848945797633	28.76672764031963
20	37.41450727290242	17.353819477892305	20.508621520084773	24.72305172912051
21	30.338648297124138	19.65171732742425	18.177657883327715	31.831976492123896
22	34.9764468371467	16.439146318015766	16.49923091713132	32.08517592770621
23	41.534321616491674	18.646836933278873	17.03947526489032	22.779366185339132
24	30.57680280944821	26.276037908308076	16.062923942848894	27.084235339394812
25	37.04700567144093	19.60972796308757	16.442372392579063	26.900893972892437
26	35.813148788927336	23.37322183775471	16.491253364090735	24.32237600922722
27	32.0141291810842	24.718858131487888	19.057574009996156	24.20943867743176
28	37.5480584390619	21.15051903114187	18.29825067281815	23.003171856978085
29	33.30129283414235	21.13711731628779	20.829528524054407	24.73206132551545
30	36.72115384615385	20.247596153846153	18.884615384615387	24.146634615384617
31	34.76568132660418	19.13722662821437	22.0620043258832	24.035087719298247
32	32.58597073030063	21.301035734025426	21.19289645062841	24.92009708504554
33	35.42007113332693	19.109872152263772	22.47909256945112	22.990964144958184
34	31.249849806550838	21.671112392761877	23.002427126138468	24.076610674548817
35	33.00012014898474	23.282470263126275	20.463775081100565	23.25363450678842
36	37.309845954194806	18.533560837278603	22.044651654610558	22.111941553916033
37	32.2063629373318	20.256632064590544	23.30113417916186	24.235870818915803
38	33.712212236266645	17.86658336136877	24.765703849665975	23.655500552698612
39	33.54801499567432	18.523502835720464	25.358069787561284	22.57041238104393
40	28.15237270189624	28.580710366734046	23.329964385407642	19.936952545962075
41	32.46100978059741	15.603297046596015	25.033042558815755	26.90265061399082
42	25.970249681589884	18.088097469540767	31.552639800062483	24.389013048806863
43	26.679642445213382	15.205690119184927	29.16426374471357	28.95040369088812
44	26.388254799721267	15.315856500949131	31.95809404810534	26.33779465122426
45	25.92993079584775	16.296616685890044	34.55642060745867	23.217031910803538
46	25.6908068624153	18.57849968763516	28.821663703205342	26.909029746744196
47	30.059109039357967	18.311788168580904	30.078331491181697	21.550771300879425
48	29.540102840117257	21.61324426930655	25.767696669710222	23.07895622086597
49	28.05689709027127	20.44979456498234	26.526346139984142	24.966962204762247
50	28.829564841290818	22.834418627003387	23.483191964822066	24.852824566883726
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	2.5
12	3.0
13	6.0
14	9.0
15	5.5
16	2.0
17	18.0
18	34.0
19	42.0
20	50.0
21	36.5
22	23.0
23	60.5
24	98.0
25	117.0
26	136.0
27	174.5
28	213.0
29	225.0
30	237.0
31	270.5
32	304.0
33	437.5
34	571.0
35	644.0
36	717.0
37	777.5
38	838.0
39	835.0
40	832.0
41	981.0
42	1130.0
43	1196.5
44	1263.0
45	1396.5
46	1530.0
47	1830.0
48	2130.0
49	2351.0
50	2572.0
51	2542.5
52	2513.0
53	2585.0
54	2657.0
55	2729.5
56	2802.0
57	2716.0
58	2630.0
59	2622.5
60	2615.0
61	2404.0
62	2193.0
63	2156.0
64	2119.0
65	1984.5
66	1850.0
67	1847.0
68	1844.0
69	1697.5
70	1551.0
71	1442.5
72	1334.0
73	1226.5
74	1119.0
75	1059.0
76	999.0
77	908.0
78	817.0
79	707.5
80	598.0
81	553.0
82	508.0
83	413.5
84	319.0
85	270.5
86	222.0
87	167.5
88	113.0
89	87.5
90	62.0
91	50.5
92	39.0
93	24.0
94	9.0
95	8.0
96	7.0
97	9.0
98	11.0
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12250780687004564
2	0.009608455440787894
3	0.0024021138601969735
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0024021138601969735
12	0.0
13	0.004804227720393947
14	0.01441268316118184
15	0.01441268316118184
16	0.17535431179437905
17	0.004804227720393947
18	0.1705500840739851
19	0.1969733365361518
20	0.25462406918087915
21	0.26903675234206104
22	0.05284650492433341
23	0.021619024741772762
24	0.13451837617103052
25	0.043238049483545524
26	0.03362959404275763
27	0.03362959404275763
28	0.03362959404275763
29	0.03843382176315158
30	0.0720634158059092
31	0.048042277203939464
32	0.04083593562334855
33	0.043238049483545524
34	0.04083593562334855
35	0.0360317079029546
36	0.0456401633437425
37	0.03362959404275763
38	0.03843382176315158
39	0.043238049483545524
40	0.17775642565457603
41	0.04083593562334855
42	0.04083593562334855
43	0.03362959404275763
44	0.031227480182560653
45	0.03362959404275763
46	0.02882536632236368
47	0.02882536632236368
48	0.02882536632236368
49	0.026423252462166705
50	0.031227480182560653
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	41630.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.88926255104492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.80493199159571	84.98198414604853
2	0.6496737808249475	1.1289935142925775
3	0.16034501824615724	0.4179678116742734
4	0.11887647904456486	0.4131635839538794
5	0.13546389472520182	0.5885178957482585
6	0.1299347561649895	0.6773961085755464
7	0.12164104832467103	0.7398510689406678
8	0.08846621696339711	0.6149411482104252
9	0.13546389472520182	1.0593322123468651
>10	0.6441446422647351	8.726879654095605
>50	0.011058277120424639	0.6509728561133797
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA	76	0.18256065337497	No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATC	70	0.16814797021378813	No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG	67	0.1609416286331972	No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG	58	0.13932260389142445	No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC	44	0.10569300984866682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16814797021378813	0.0
2	0.0	0.0	0.0	0.16814797021378813	0.0
3	0.0	0.0	0.0	0.19216910881575786	0.0
4	0.0	0.0	0.0	0.20417967811674273	0.0
5	0.0	0.0	0.0	0.2161902474177276	0.0
6	0.0	0.0	0.0	0.2161902474177276	0.0
7	0.0	0.0	0.0	0.2161902474177276	0.0
8	0.0	0.0	0.0	0.2161902474177276	0.0
9	0.0	0.0	0.0	0.21859236127792459	0.0
10	0.0	0.0	0.0	0.27384098006245494	0.0
11	0.0	0.0	0.0	0.36752342061013693	0.0
12	0.0	0.0	0.0	0.41076147009368247	0.0
13	0.0	0.0	0.0	0.4155656978140764	0.0
14	0.0	0.0	0.0	0.4155656978140764	0.0
15	0.0	0.0	0.0	0.4155656978140764	0.0
16	0.0	0.0	0.0	0.4419889502762431	0.0
17	0.0	0.0	0.0	0.4419889502762431	0.0
18	0.0	0.0	0.0	0.6533749699735768	0.0
19	0.0	0.0	0.0	0.8239250540475619	0.0
20	0.0	0.0	0.0	0.9128032668748499	0.0
21	0.0	0.0	0.0	0.9152053807350469	0.0
22	0.0	0.0	0.0	0.9488349747778044	0.0
23	0.0	0.0	0.0	1.0088878212827288	0.0
24	0.0	0.0	0.0	1.0305068460245015	0.0
25	0.0	0.0	0.0	1.0329089598846986	0.0
26	0.0	0.0	0.0	1.0329089598846986	0.0
27	0.0	0.0	0.0	1.1097766034110017	0.0
28	0.0	0.0	0.0	1.1770357914965168	0.0
29	0.0	0.0	0.0	1.2106653855392746	0.0
30	0.0	0.0	0.0	1.2394907518616383	0.0
31	0.0	0.0	0.0	1.2442949795820322	0.0
32	0.0	0.0	0.0	1.2971414845063656	0.0
33	0.0	0.0	0.0	1.4004323804948355	0.0
34	0.0	0.0	0.0	1.4724957963007446	0.0
35	0.0	0.0	0.0	1.6862839298582752	0.0
36	0.0	0.0	0.0	1.9721354792217152	0.0
37	0.0	0.0	0.0	2.3060293057890946	0.0
38	0.0	0.0	0.0	2.97621907278405	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	35	7.1653376E-6	37.681316	1
TAAGCGG	30	1.2862596E-4	36.634617	1
AGCCACG	50	5.6972567E-8	35.21155	27
ATCGGGC	25	0.0023230824	35.21155	27
CGTCAGA	25	0.002336862	35.169228	2
TTTACAC	55	1.3305544E-7	31.972029	2
CTTTACA	55	1.3305544E-7	31.972029	1
CAATGTG	35	3.1814398E-4	31.401098	32
CGGGTCG	30	0.005639742	29.378313	12
GATCGGG	30	0.005673108	29.342962	26
GAGCCAC	45	3.967979E-5	29.342962	26
CCGACGC	45	3.967979E-5	29.342962	18
GCCACGG	30	0.005673108	29.342962	28
GAGATCT	30	0.005673108	29.342962	39
GACGAAA	45	4.000788E-5	29.307693	23
GTCAGAT	30	0.0057066297	29.307692	3
TCAGATG	30	0.0057066297	29.307692	4
GCGTCAG	30	0.0057066297	29.307692	1
AGAACGG	40	6.904481E-4	27.509024	27
AATGTGT	40	6.904481E-4	27.509024	33
>>END_MODULE