FastQCFastQC Report
Wed 25 May 2016
SRR1780661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41630
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2048749.21210665385539No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT493511.854431900072065TruSeq Adapter, Index 10 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG26026.250300264232525No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC24835.964448714869085No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC6331.5205380735046843No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT5411.2995435983665626No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC4321.0377131876050925No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT2280.5476819601249099No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC2220.5332692769637281No Hit
ATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCTT2010.4828248858995916TruSeq Adapter, Index 10 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCT1710.4107614700936824RNA PCR Primer, Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCT1710.4107614700936824TruSeq Adapter, Index 10 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC1590.38193610377131876No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTATCTCGTATGCCGTC1160.2786452077828489No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC980.23540715829930337No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG920.22099447513812157No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA760.18256065337497No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTA710.1705500840739851No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGTCTT700.16814797021378813No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATTTCGTATGCCGT630.15133317319240933No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG620.14893105933221235No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG600.1441268316118184No Hit
TCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCTTG540.12971414845063656TruSeq Adapter, Index 10 (96% over 28bp)
CTTATCACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC530.12731203459043958No Hit
CTTATAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG500.12010569300984868No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG450.10809512370886379No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCG430.10329089598846986No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCC420.10088878212827289No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGC420.10088878212827289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA302.4515066E-644.0457271
CTTTACA502.5465852E-1144.0457271
TATAACA207.729451E-444.0457273
TTATAAC207.729451E-444.0457272
ATAACAC207.729451E-444.0457274
CTTATAA351.3901627E-744.0457271
TCTTTAT302.4515066E-644.0457271
TTTACAC502.5465852E-1144.0457272
TTACACA557.2759576E-1140.0415733
CCGTCTT5450.038.3423444
ACATCTG250.002328729535.194238
AACATCT250.002328729535.194237
CTTATAC29200.034.9198151
TTATACA29300.034.8006362
TATACAC30200.033.763533
GCCGTCT6200.033.7041543
ATGCCGT29900.033.61990744
TATGCCG32750.030.69420443
TGCCGTC7050.029.64053242
TAACACA300.00565343829.363825
Produced by FastQC (version 0.11.5)