FastQCFastQC Report
Wed 25 May 2016
SRR1780660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5662
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT214037.795831861533024No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT4367.700459201695514Illumina PCR Primer Index 9 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2845.015895443306253No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2294.044507241257507No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1242.190038855528082No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC811.4305898975626987No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT801.412928293889085No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT641.130342635111268No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC500.8830801836806783No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT480.8477569763334512Illumina PCR Primer Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCG280.49452490286117984No Hit
CTTATACACATCTCCGAGCCCACGCGACGTTAACAGATCTCGTATGCCGT250.44154009184033916No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCG230.40621688449311194No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCTT200.35323207347227126No Hit
ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT200.35323207347227126Illumina PCR Primer Index 9 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG200.35323207347227126No Hit
CTTATACACATCTCCGAGCCCACGAGCCGTTAACAGATCTCGTATGCCGT180.31790886612504415No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC180.31790886612504415No Hit
CTTATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGT170.30024726245143063No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACATCGTATGCCGT170.30024726245143063No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC170.30024726245143063No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT160.282585658777817No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT160.282585658777817No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGT160.282585658777817No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC140.24726245143058992No Hit
CTTATACACATCTCCGAGCCCACGAGAAGTTAACAGATCTCGTATGCCGT140.24726245143058992No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGTCTT140.24726245143058992No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT130.2296008477569763No Hit
ATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTGCTT120.2119392440833628Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACCGATCTCGTATGCCGT120.2119392440833628No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT120.2119392440833628No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATTTCGTATGCCGT110.1942776404097492No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCC110.1942776404097492No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAACAGATCTCGTATGCCGT110.1942776404097492No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT110.1942776404097492No Hit
CTTATACACATCTCCGCGCCCACGAGACGTTAACAGATCTCGTATGCCGT110.1942776404097492No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAAATCGTATGCCGT100.17661603673613563No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG100.17661603673613563No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT100.17661603673613563No Hit
CTTATACACATCTCCGAGCCCAAGAGACGTTAACAGATCTCGTATGCCGT90.15895443306252208No Hit
CTTATACACATATCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT90.15895443306252208No Hit
CTTAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC90.15895443306252208No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTAGTATGCCGT90.15895443306252208No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCACAGATCTCGTATGCCGT80.1412928293889085No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTA80.1412928293889085No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTCTGCCGT80.1412928293889085No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC80.1412928293889085No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCACGTATGCCGT80.1412928293889085No Hit
CTTATACACATCTACGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT80.1412928293889085No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA80.1412928293889085No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCC80.1412928293889085No Hit
CTTATACACATCTCCGAGCCCACGTGACGTTAACAGATCTCGTATGCCGT70.12363122571529496No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCCCGTATGCCGT70.12363122571529496No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAAATCGTATGCCG60.1059696220416814No Hit
ATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGTCTT60.1059696220416814Illumina PCR Primer Index 9 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGAAGTTAACAGATCTCGTATGCCGTCTT60.1059696220416814TruSeq Adapter, Index 9 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTCTGCCGTCTT60.1059696220416814No Hit
GTATAATGTTTACATATTTGTGAAATAATGTTTTATACTGTTTATGGATA60.1059696220416814No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCAACAGATCTCGTATGCCGT60.1059696220416814No Hit
AGATTAATTAGTTCAAAAATCAAACAGCTAATGAGTAAAAGTGCCTGGAT60.1059696220416814No Hit
GCTGGACAGGGTCTTTTCGATGAGTTCCCGCAGCGCAAAGCTTGATGGGA60.1059696220416814No Hit
CTTATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT60.1059696220416814No Hit
TCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT60.1059696220416814No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGATA150.002111759770.0000133
GATATCG150.002111759770.0000136
CAGATAT150.002111759770.0000134
TTGAACA150.002111759770.0000158
CTTGAAC150.002111759770.0000157
TGAACAA150.002111759770.0000159
ATATCGT150.002111759770.0000137
AGATATC150.002111759770.0000135
CTTTACA252.4919646E-670.01
TATCGTA207.266711E-570.038
TTACACA252.4919646E-670.03
ATCGTAT252.4919646E-670.039
TTTACAC252.4919646E-670.02
ACTCGTA252.1925599E-455.99999638
AGAACTC252.1925599E-455.99999635
AACTCGT252.1925599E-455.99999637
GAACTCG252.1925599E-455.99999636
CAGAACT252.1925599E-455.99999634
TTATACA4250.053.529412
CTTATAC4200.053.3333321
Produced by FastQC (version 0.11.5)