##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780660_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5662 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5503355704698 34.0 34.0 34.0 31.0 34.0 2 33.01112681031438 34.0 34.0 34.0 32.0 34.0 3 33.369480748852 34.0 34.0 34.0 33.0 34.0 4 33.46714941716708 34.0 34.0 34.0 34.0 34.0 5 33.50211939244083 34.0 34.0 34.0 34.0 34.0 6 37.33945602260685 38.0 37.0 38.0 36.0 38.0 7 36.7629812787001 38.0 37.0 38.0 34.0 38.0 8 36.89438361003179 38.0 37.0 38.0 35.0 38.0 9 36.67502649240551 38.0 37.0 38.0 34.0 38.0 10-11 36.38943836100318 38.0 37.0 38.0 34.0 38.0 12-13 36.08716001412928 38.0 37.0 38.0 33.0 38.0 14-15 36.11091487107029 38.0 36.5 38.0 32.5 38.0 16-17 35.87831155068881 38.0 36.0 38.0 31.5 38.0 18-19 35.93332744613211 38.0 36.5 38.0 32.0 38.0 20-21 35.694542564464854 38.0 36.0 38.0 31.0 38.0 22-23 36.10720593429883 38.0 36.5 38.0 32.5 38.0 24-25 34.61930413281526 37.0 34.5 38.0 27.0 38.0 26-27 34.624161073825505 37.0 34.0 38.0 26.0 38.0 28-29 35.087424938184384 37.0 34.5 38.0 31.0 38.0 30-31 35.00529848110209 37.0 34.5 38.0 29.0 38.0 32-33 35.42537972447899 37.5 36.0 38.0 31.0 38.0 34-35 34.89985870717061 37.0 35.0 38.0 28.5 38.0 36-37 34.54159307665136 37.0 34.5 38.0 26.5 38.0 38-39 29.54750971388202 34.0 25.0 37.0 11.0 38.0 40-41 32.352702225362066 36.0 30.5 37.0 18.0 38.0 42-43 34.834157541504766 37.0 34.5 38.0 29.0 38.0 44-45 35.29380077711056 37.0 35.5 38.0 31.0 38.0 46-47 35.584334157541505 38.0 36.0 38.0 31.0 38.0 48-49 35.06826209819852 37.5 35.0 38.0 28.5 38.0 50-51 34.8658601200989 37.0 34.5 38.0 29.0 38.0 52-53 34.42184740374426 37.0 34.0 38.0 25.5 38.0 54-55 34.96388202048746 37.0 35.5 38.0 27.0 38.0 56-57 35.057311903920876 37.0 35.5 38.0 27.0 38.0 58-59 34.497615683504065 37.0 34.0 38.0 26.0 38.0 60-61 34.18968562345461 37.0 34.0 38.0 25.0 38.0 62-63 35.05642882373719 37.0 35.0 38.0 29.0 38.0 64-65 35.19516072059343 37.0 36.0 38.0 29.0 38.0 66-67 33.31367008124337 37.0 32.5 38.0 22.0 38.0 68-69 30.44604380077711 35.0 24.5 37.5 11.0 38.0 70-71 26.841222182974214 29.5 17.0 35.5 10.5 37.5 72-73 21.51280466266337 22.0 11.0 29.5 10.5 36.5 74-75 20.825679971741433 21.0 11.0 27.5 11.0 36.0 76 21.236135641116213 21.0 11.0 30.0 11.0 36.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5516948504666388 1101 2 0.7831946562209708 1101 3 0.4413494629815702 1101 4 0.4276226550322235 1101 5 -0.8802248755625897 1101 6 -1.647173676856326 1101 7 0.16565113219933636 1101 8 0.18097772981369076 1101 9 -0.6659600072586755 1101 10-11 -0.8800037589485825 1101 12-13 0.6022475633513196 1101 14-15 0.6550014245911058 1101 16-17 -0.03333351765148507 1101 18-19 -0.747858808684029 1101 20-21 -0.1074470850221374 1101 22-23 0.7413536594554415 1101 24-25 -3.498890563431466 1101 26-27 -1.848602757220057 1101 28-29 0.3561175659656257 1101 30-31 1.2900944003763044 1101 32-33 0.30712309197419785 1101 34-35 0.21264076362098194 1101 36-37 -0.8948671375742805 1101 38-39 -0.9592170110817158 1101 40-41 2.3788432870516942 1101 42-43 0.46710128208925994 1101 44-45 0.44798247463434393 1101 46-47 1.4447093094610182 1101 48-49 -2.383626005012694 1101 50-51 -3.160190733928424 1101 52-53 -1.8029071293863765 1101 54-55 -0.35007036929088287 1101 56-57 -2.1333768113347986 1101 58-59 -1.373300413896395 1101 60-61 -3.133433096141367 1101 62-63 -5.666465227442686 1101 64-65 -1.5331557529949578 1101 66-67 -4.753137785948507 1101 68-69 -5.677246105027901 1101 70-71 -4.338566777853078 1101 72-73 -3.797348353434895 1101 74-75 -5.1333926291219125 1101 76 0.9915715115863755 1102 1 -0.8399718162000269 1102 2 0.8581946562209737 1102 3 0.5080161296482402 1102 4 0.4276226550322235 1102 5 -0.10522487556259108 1102 6 0.18199298981033962 1102 7 0.5948177988660035 1102 8 0.851811063147025 1102 9 0.9548733260746545 1102 10-11 0.8408295743847489 1102 12-13 1.4022475633513167 1102 14-15 -0.7554152420755571 1102 16-17 0.04374981568184921 1102 18-19 1.1417245246493053 1102 20-21 1.894636248311194 1102 22-23 0.5621869927887744 1102 24-25 -4.271807230098133 1102 26-27 -0.8277694238867213 1102 28-29 1.2644508992989572 1102 30-31 1.2234277337096344 1102 32-33 0.49045642530753497 1102 34-35 -0.008192569712349496 1102 36-37 -3.8552838042409405 1102 38-39 1.7824496555849514 1102 40-41 2.855926620385027 1102 42-43 1.0087679487559313 1102 44-45 0.8521491413010125 1102 46-47 1.6738759761276896 1102 48-49 -5.402376005012691 1102 50-51 -0.34769073392842387 1102 52-53 0.0637595372802906 1102 54-55 -0.6063203692908772 1102 56-57 1.141623188665207 1102 58-59 -1.4420504138963892 1102 60-61 -7.406349762808034 1102 62-63 -3.9685485607760214 1102 64-65 -2.270655752994955 1102 66-67 2.5635288807181595 1102 68-69 -0.7439127716945677 1102 70-71 -0.9219001111864138 1102 72-73 -0.15359835343489436 1102 74-75 0.6228573708780871 1102 76 0.045738178253042605 1103 1 1.226204654388205 1103 2 0.8067240679856766 1103 3 0.3315455414129431 1103 4 0.36879912562045547 1103 5 0.46095159502564087 1103 6 0.5018459309868106 1103 7 0.9734942694542355 1103 8 0.796664004323496 1103 9 0.38502038489818347 1103 10-11 0.15332957438474892 1103 12-13 0.2551887398219037 1103 14-15 0.11774652263032692 1103 16-17 -0.7117648902004987 1103 18-19 0.3384157011198923 1103 20-21 -0.2505843399240959 1103 22-23 0.3158634633770063 1103 24-25 -1.9537925242157854 1103 26-27 0.13178939964269176 1103 28-29 -0.13996086540692687 1103 30-31 1.6057806748861054 1103 32-33 1.8875152488369409 1103 34-35 1.4440133126405925 1103 36-37 -3.4085926277703535 1103 38-39 -0.6513738738268167 1103 40-41 -0.2525292619679149 1103 42-43 -0.6015261688911266 1103 44-45 1.9146491413010125 1103 46-47 1.9349053878923996 1103 48-49 -2.036567181483278 1103 50-51 1.516279854306866 1103 52-53 0.8872889490450007 1103 54-55 1.1087531601208909 1103 56-57 1.060740835724033 1103 58-59 0.8208172331624368 1103 60-61 -3.205982115749208 1103 62-63 -0.2645044431289634 1103 64-65 0.872726599946219 1103 66-67 -0.3923534722230144 1103 68-69 1.6384401694819033 1103 70-71 3.798688124107702 1103 72-73 -0.37970129461136537 1103 74-75 -0.033392629121912876 1103 76 1.270002884135394 1104 1 -0.5973247573764979 1104 2 0.6890770091621476 1104 3 0.5080161296482402 1104 4 0.2511520667969265 1104 5 0.5197751244374089 1104 6 0.26655181333975264 1104 7 0.8558472106307065 1104 8 0.737840474911728 1104 9 0.6203145025452415 1104 10-11 -1.1996116020858452 1104 12-13 0.43165932805720075 1104 14-15 0.8530406402773849 1104 16-17 0.6411762862700812 1104 18-19 0.45606275994342127 1104 20-21 -1.309407869335864 1104 22-23 0.9335105222005424 1104 24-25 -0.21849840656872033 1104 26-27 1.8376717525838728 1104 28-29 1.1835685463577832 1104 30-31 -1.7177487368785975 1104 32-33 0.12280936648400598 1104 34-35 0.12048390087588245 1104 36-37 1.6796426663472914 1104 38-39 2.583920243820245 1104 40-41 3.218058973326201 1104 42-43 2.0749444193441633 1104 44-45 1.5322962001245415 1104 46-47 0.9643171525982766 1104 48-49 1.081079877340251 1104 50-51 1.516279854306866 1104 52-53 1.1519948313979356 1104 54-55 1.7852237483561808 1104 56-57 -0.40984739957008287 1104 58-59 -3.2380062962493312 1104 60-61 -0.5589232922198022 1104 62-63 -1.7645044431289634 1104 64-65 -0.24492045887730995 1104 66-67 1.0488229983652175 1104 68-69 3.4325578165407222 1104 70-71 2.063394006460644 1104 72-73 -0.46793658872901034 1104 74-75 0.34896031205455813 1104 76 1.3876499429589266 1105 1 1.2323966048526032 1105 2 0.6147736035893914 1105 3 0.5080161296482402 1105 4 0.05920160240064121 1105 5 -0.37496171766785125 1105 6 -0.27524385229492054 1105 7 0.7165283251817911 1105 8 -0.4541099894845573 1105 9 0.8989522734430722 1105 10-11 0.7849085217531737 1105 12-13 -1.3609103313855258 1105 14-15 0.14096633687181281 1105 16-17 0.30526297357658905 1105 18-19 0.9328429457019354 1105 20-21 0.952202037784879 1105 22-23 -0.02004984931648579 1105 24-25 0.8712848751650242 1105 26-27 1.7478884708501212 1105 28-29 1.521029846667382 1105 30-31 0.933954049499107 1105 32-33 0.4772985305706925 1105 34-35 -1.6891136223439247 1105 36-37 -0.07732327792514582 1105 38-39 0.06534439242705758 1105 40-41 -0.435191800667603 1105 42-43 0.26534689612435614 1105 44-45 1.2567544044589027 1105 46-47 0.4008496603382099 1105 48-49 -3.157310215539006 1105 50-51 -1.607559154981054 1105 52-53 -0.12045098903550411 1105 54-55 0.1288769991301777 1105 56-57 -0.9965347060716283 1105 58-59 -0.5259319928437591 1105 60-61 -0.2632576575448766 1105 62-63 -1.0261143502497063 1105 64-65 -0.4647347003633797 1105 66-67 0.12931835440236483 1105 68-69 -1.322860140115619 1105 70-71 1.9596788361820074 1105 72-73 -1.3394536165927917 1105 74-75 -1.1123399975429678 1105 76 0.12139607298988508 1106 1 0.7225281837999731 1106 2 0.9831946562209737 1106 3 0.5080161296482402 1106 4 0.4276226550322235 1106 5 0.3322751244374089 1106 6 0.3694929898103396 1106 7 -2.7801822011339965 1106 8 0.539311063147025 1106 9 -0.8576266739253455 1106 10-11 1.309579574384749 1106 12-13 1.8709975633513167 1106 14-15 0.8383347579244429 1106 16-17 1.4187498156818492 1106 18-19 1.3604745246493053 1106 20-21 0.769636248311194 1106 22-23 1.1246869927887744 1106 24-25 -0.053057230098133346 1106 26-27 2.4222305761132787 1106 28-29 1.3269508992989572 1106 30-31 1.4734277337096344 1106 32-33 0.521706425307535 1106 34-35 -1.0394425697123495 1106 36-37 1.8634661957590595 1106 38-39 5.719949655584951 1106 40-41 2.137176620385027 1106 42-43 2.3837679487559313 1106 44-45 2.6333991413010125 1106 46-47 2.2988759761276896 1106 48-49 2.035123994987309 1106 50-51 0.49605926607157613 1106 52-53 2.1887595372802906 1106 54-55 0.9874296307091228 1106 56-57 2.047873188665207 1106 58-59 2.495449586103611 1106 60-61 3.281150237191966 1106 62-63 2.3439514392239786 1106 64-65 -2.083155752994955 1106 66-67 2.3135288807181595 1106 68-69 2.5060872283054323 1106 70-71 3.265599888813586 1106 72-73 2.4401516465651056 1106 74-75 3.029107370878087 1106 76 1.2332381782530426 1107 1 1.0628059615777516 1107 2 -2.683472010445694 1107 3 -1.6030949814628705 1107 4 -0.07237734496777648 1107 5 0.1308862355485232 1107 6 0.3972707675881182 1107 7 0.42120668775488923 1107 8 0.1920888409248036 1107 9 -0.6770711183697884 1107 10-11 0.29221846327363465 1107 12-13 0.6522475633513167 1107 14-15 0.005001424591114301 1107 16-17 1.4499998156818492 1107 18-19 1.2910300802048624 1107 20-21 1.675886248311194 1107 22-23 1.0691314372332172 1107 24-25 0.551109436568531 1107 26-27 -1.2374916461089427 1107 28-29 0.8894508992989572 1107 30-31 -0.4154611551792513 1107 32-33 0.7682342030853135 1107 34-35 1.262640763620979 1107 36-37 2.071799529092388 1107 38-39 -0.2314392333039379 1107 40-41 2.0677321759405842 1107 42-43 0.38376794875593134 1107 44-45 0.9424269190787911 1107 46-47 1.7224870872388038 1107 48-49 0.8441517727650876 1107 50-51 2.0620314882937976 1107 52-53 1.9248706483914049 1107 54-55 2.35548518626468 1107 56-57 1.3221787442207642 1107 58-59 1.8044773638813894 1107 60-61 1.4443446816364087 1107 62-63 1.3335347725573072 1107 64-65 2.1390664692272665 1107 66-67 0.23019554738483095 1107 68-69 0.3949761171943216 1107 70-71 -4.671900111186414 1107 72-73 -2.4973483534348944 1107 74-75 -2.1445037402330236 1107 76 -2.919539599524736 1108 1 -0.0679129926706139 1108 2 0.9243711268092056 1108 3 0.5080161296482402 1108 4 0.4276226550322235 1108 5 0.5197751244374089 1108 6 0.5018459309868106 1108 7 0.32643544592482954 1108 8 -1.968041878029446 1108 9 0.6203145025452415 1108 10-11 0.976858986149459 1108 12-13 1.4316593280572008 1108 14-15 0.9412759343950299 1108 16-17 0.023529227446559275 1108 18-19 -0.10276076946834678 1108 20-21 0.9847097777229621 1108 22-23 -0.06648947779945757 1108 24-25 2.2226780640195187 1108 26-27 1.0141423408191628 1108 28-29 -1.3164314536422168 1108 30-31 0.6057806748861054 1108 32-33 -0.28895533939834905 1108 34-35 -0.26186904030058145 1108 36-37 1.0031720781120015 1108 38-39 0.5839202438202484 1108 40-41 2.276882502737969 1108 42-43 0.4572973605206414 1108 44-45 1.5617079648304184 1108 46-47 1.6996112702453345 1108 48-49 1.963432818516722 1108 50-51 2.075103383718634 1108 52-53 1.9167007137508776 1108 54-55 0.49110610129735477 1108 56-57 0.35485848278285204 1108 58-59 1.5855231155153788 1108 60-61 1.35284141366256 1108 62-63 1.8825543804004496 1108 64-65 2.049197188181509 1108 66-67 2.5488229983652175 1108 68-69 2.8149107577172003 1108 70-71 -0.9071942288334718 1108 72-73 -2.93852482402313 1108 74-75 -0.9745690997101484 1108 76 -1.3770559393940154 1109 1 0.6600281837999731 1109 2 0.3581946562209737 1109 3 0.44551612964824017 1109 4 0.4276226550322235 1109 5 -0.9802248755625911 1109 6 -1.8805070101896604 1109 7 0.2823177988660035 1109 8 0.539311063147025 1109 9 0.7673733260746545 1109 10-11 0.6533295743847489 1109 12-13 0.7772475633513167 1109 14-15 1.088334757924443 1109 16-17 1.6687498156818492 1109 18-19 1.2042245246493053 1109 20-21 1.457136248311194 1109 22-23 0.09343699278877438 1109 24-25 0.7594427699018667 1109 26-27 0.7972305761132787 1109 28-29 -0.5167991007010428 1109 30-31 -0.026572266290365576 1109 32-33 1.865456425307535 1109 34-35 1.7418074302876505 1109 36-37 1.3009661957590595 1109 38-39 3.0011996555849514 1109 40-41 -0.4565733796149729 1109 42-43 1.2275179487559313 1109 44-45 -0.4291008586989875 1109 46-47 1.7363759761276896 1109 48-49 1.597623994987309 1109 50-51 0.33980926607157613 1109 52-53 -0.6549904627197094 1109 54-55 -1.6688203692908772 1109 56-57 0.32912318866520707 1109 58-59 -0.3483004138963892 1109 60-61 1.1874002371919659 1109 62-63 0.9064514392239786 1109 64-65 0.6355942470050451 1109 66-67 1.6260288807181595 1109 68-69 2.9123372283054323 1109 70-71 3.953099888813586 1109 72-73 3.0339016465651056 1109 74-75 3.622857370878087 1109 76 3.5457381782530426 1110 1 -2.089971816200027 1110 2 0.4831946562209737 1110 3 0.32051612964824017 1110 4 0.4276226550322235 1110 5 0.5197751244374089 1110 6 0.5569929898103396 1110 7 0.9698177988660035 1110 8 0.789311063147025 1110 9 0.7673733260746545 1110 10-11 1.059579574384749 1110 12-13 0.7459975633513167 1110 14-15 1.369584757924443 1110 16-17 2.012499815681849 1110 18-19 1.0167245246493053 1110 20-21 0.800886248311194 1110 22-23 0.24968699278877438 1110 24-25 0.25944276990186665 1110 26-27 2.8284805761132787 1110 28-29 1.3894508992989572 1110 30-31 0.16092773370963442 1110 32-33 1.990456425307535 1110 34-35 2.6168074302876505 1110 36-37 3.5509661957590595 1110 38-39 2.5324496555849514 1110 40-41 1.668426620385027 1110 42-43 0.5712679487559313 1110 44-45 1.3833991413010125 1110 46-47 1.8926259761276896 1110 48-49 1.753873994987309 1110 50-51 0.12105926607157613 1110 52-53 2.7825095372802906 1110 54-55 1.2999296307091228 1110 56-57 2.485373188665207 1110 58-59 0.4016995861036108 1110 60-61 2.531150237191966 1110 62-63 -0.5622985607760214 1110 64-65 1.166844247005045 1110 66-67 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74-75 -0.3191069148361976 2116 76 -0.28461896460409974 2117 1 1.285028183799973 2117 2 0.8013764744027938 2117 3 -0.12834750671539297 2117 4 0.33671356412313 2117 5 0.24704785171013555 2117 6 0.25585662617397276 2117 7 0.6686814352296366 2117 8 0.6415837904197517 2117 9 -1.3689903102889787 2117 10-11 -0.4830340619788913 2117 12-13 -1.0068433457395898 2117 14-15 -1.3207561511664636 2117 16-17 -5.254545638863604 2117 18-19 -1.6054345662597882 2117 20-21 -2.1195682971433527 2117 22-23 -0.21622209812031912 2117 24-25 -3.77464813918904 2117 26-27 -3.242542151159448 2117 28-29 -2.5196400097919494 2117 30-31 -0.7311177208358188 2117 32-33 -5.259543574692465 2117 34-35 0.770216521196744 2117 36-37 -2.034261076968214 2117 38-39 -1.0925503444150486 2117 40-41 -2.896914288705883 2117 42-43 -0.9571411421531621 2117 44-45 -3.0398963132444408 2117 46-47 1.520466885218596 2117 48-49 0.08910126771458238 2117 50-51 -0.8420089157466109 2117 52-53 0.8819413554621036 2117 54-55 1.6408387216182163 2117 56-57 0.4029868250288473 2117 58-59 -3.7460276866236626 2117 60-61 -0.5000997628080341 2117 62-63 0.31838325740579165 2117 64-65 -0.7877012075404082 2117 66-67 -0.4137438465545671 2117 68-69 0.21063268285088554 2117 70-71 -0.12644556573186705 2117 72-73 -1.042802898889441 2117 74-75 0.01206191633263387 2117 76 0.8582381782530426 2118 1 -2.945741046969257 2118 2 -0.6321899591636395 2118 3 -0.18429156265945323 2118 4 0.11993034733991692 2118 5 -0.01868641402413118 2118 6 0.5425699128872594 2118 7 0.6477024142506167 2118 8 0.6066187554547184 2118 9 -2.8095497508484257 2118 10-11 -0.4620550409998643 2118 12-13 -0.09775243664868327 2118 14-15 -2.373203703614017 2118 16-17 -7.261538645856611 2118 18-19 -1.9376023984276145 2118 20-21 -0.38180605938111256 2118 22-23 -0.5099283918266124 2118 24-25 1.0959812314403266 2118 26-27 -6.399884808502108 2118 28-29 -0.8797798699318164 2118 30-31 -2.507341497059599 2118 32-33 -1.5672358823847716 2118 34-35 -1.3976156466354297 2118 36-37 -3.7615338042409405 2118 38-39 -4.746396498261202 2118 40-41 -0.6032079949995861 2118 42-43 0.47992179490977094 2118 44-45 -3.239197012545141 2118 46-47 -0.8501624854107774 2118 48-49 -0.4528567742434646 2118 50-51 -1.275575349313037 2118 52-53 -5.013163539642786 2118 54-55 -4.219301138521647 2118 56-57 1.766623188665207 2118 58-59 -1.857915798511776 2118 60-61 -0.26933053203880064 2118 62-63 -2.384413945391408 2118 64-65 1.666844247005045 2118 66-67 -1.7249326577433806 2118 68-69 -1.666989694771491 2118 70-71 -1.5949770342633336 2118 72-73 1.8488054927189523 2118 74-75 -0.8795464752757596 2118 76 -0.449454129439264 2119 1 0.746566645338433 2119 2 -2.9398822668559497 2119 3 -0.49198387035175983 2119 4 -1.1877619603523897 2119 5 -0.6340710294087444 2119 6 -2.3805070101896604 2119 7 -1.1215283549801498 2119 8 -0.08568893685297496 2119 9 -1.3480112893099587 2119 10-11 -0.7697473486921709 2119 12-13 -0.02082935972560307 2119 14-15 -0.3347421651524769 2119 16-17 -1.953846338164304 2119 18-19 -2.0145254753506947 2119 20-21 -1.9971906747657258 2119 22-23 -1.279159161057379 2119 24-25 0.48059661605571335 2119 26-27 -1.0152694238867213 2119 28-29 -1.3797798699318164 2119 30-31 -0.6227261124442123 2119 32-33 0.08661027146138167 2119 34-35 0.4869997379799571 2119 36-37 -0.6461491888563273 2119 38-39 -2.746396498261202 2119 40-41 -2.218592610384203 2119 42-43 -4.597001282013302 2119 44-45 -1.4699662433143743 2119 46-47 -1.2347778700261571 2119 48-49 -1.722087543474224 2119 50-51 -0.006344580082270568 2119 52-53 -1.5131635396427896 2119 54-55 -1.6423780615985706 2119 56-57 -4.925684503642486 2119 58-59 -2.434838875434849 2119 60-61 -2.3077920705003407 2119 62-63 -0.15364471462217466 2119 64-65 -0.4100788299180351 2119 66-67 -1.8018557346664537 2119 68-69 0.2945487667669724 2119 70-71 -1.9026693419556437 2119 72-73 0.425728569642029 2119 74-75 0.12045352472424042 2119 76 0.3197766397915025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 1.0 23 5.0 24 3.0 25 24.0 26 33.0 27 70.0 28 123.0 29 203.0 30 319.0 31 472.0 32 614.0 33 807.0 34 1162.0 35 1219.0 36 401.0 37 205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.680324973507594 14.111621335217237 11.99222889438361 69.21582479689155 2 2.7375485694101025 3.7795831861533027 81.40233133168492 12.080536912751679 3 2.808194984104557 15.489226421759096 72.23595902507948 9.46661956905687 4 1.7308371600141295 59.13104909925821 23.825503355704697 15.312610385022959 5 2.225362062875309 27.658071352878842 66.30166019074531 3.81490639350053 6 3.2497350759448955 67.85588131402332 12.062875309078064 16.831508300953725 7 3.461674320028259 27.64040974920523 9.46661956905687 59.43129636170964 8 2.5256093253267395 67.66160367361357 14.500176616036736 15.312610385022959 9 3.1967502649240553 15.11833274461321 4.327092900035323 77.35782409042741 10-11 3.240904274108089 38.25503355704698 38.85552808194984 19.64853408689509 12-13 8.239138113740728 6.790886612504416 40.957258919109854 44.012716354645 14-15 10.623454609678559 9.051571882726952 11.082656305192511 69.24231720240198 16-17 36.91275167785235 33.30095372659837 3.2055810667608617 26.580713528788415 18-19 38.81137407276581 16.566584245849523 3.0201342281879198 41.60190745319675 20-21 12.177675732956553 6.561285764747439 3.0642882373719535 78.19675026492405 22-23 10.535146591310491 41.75203108442246 3.4263511126810315 44.28647121158601 24-25 37.59272341928647 41.858000706464146 3.6559519604380077 16.893323913811372 26-27 44.2776404097492 40.70116566584246 8.795478629459556 6.225715294948781 28-29 35.57930060049452 17.14941716707877 11.859766866831508 35.4115153655952 30-31 11.241610738255034 11.930413281525963 64.22642175909573 12.60155422112328 32-33 8.804309431296362 69.90462734016248 14.924055104203463 6.367008124337691 34-35 5.174849876368774 50.10596962204168 9.201695513952666 35.51748498763688 36-37 34.57258919109855 40.97492052278347 11.276933945602261 13.175556340515717 38-39 12.071706110914871 15.11833274461321 34.643235605793 38.16672553867891 40-41 5.87248322147651 9.71388202048746 44.18050158954433 40.2331331684917 42-43 40.06534793359237 5.245496291063229 42.67926527728718 12.009890498057224 44-45 8.468738961497705 34.56375838926174 41.858000706464146 15.109501942776404 46-47 40.52454962910632 6.34051571882727 17.811727304839277 35.323207347227125 48-49 35.56163899682091 3.2320734722712823 12.001059696220418 49.205227834687385 50-51 10.190745319675026 3.5323207347227132 44.51607205934299 41.76086188625927 52-53 7.824090427410808 3.2320734722712823 74.50547509713881 14.43836100317909 54-55 4.530201342281879 3.8060755916637232 50.30907806428824 41.35464500176616 56-57 38.917343694807485 4.3359237018721295 20.293182620981984 36.453549982338394 58-59 7.718120805369128 15.692334864005652 64.76510067114094 11.824443659484281 60-61 34.13104909925821 46.82974214058637 14.54433062522077 4.494878134934653 62-63 5.704697986577181 86.54185800070647 3.0907806428823736 4.662663369833981 64-65 2.0310844224655598 89.8269162839986 2.9671494171670787 5.174849876368774 66-67 2.0929000353232072 88.24620275521016 2.8523489932885906 6.808548216178028 68-69 2.5432709290003532 83.38043094312964 4.035676439420699 10.040621688449312 70-71 3.673613564111621 75.50335570469798 5.37795831861533 15.445072412575062 72-73 5.042387848816673 60.102437301306956 10.393853761921584 24.461321087954786 74-75 5.65171317555634 57.5503355704698 9.872836453549983 26.925114800423877 76 4.221123277993642 59.96114447191805 9.51960438007771 26.298127870010596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 5.5 23 5.5 24 1.0 25 2.0 26 3.5 27 3.5 28 2.0 29 2.0 30 1.5 31 1.0 32 9.5 33 18.0 34 19.0 35 18.5 36 25.5 37 34.0 38 95.5 39 468.5 40 1057.5 41 1303.0 42 1271.0 43 1049.0 44 639.5 45 344.0 46 236.0 47 170.0 48 81.0 49 51.5 50 45.0 51 42.5 52 38.0 53 29.0 54 22.0 55 27.0 56 31.0 57 24.5 58 19.0 59 18.5 60 17.0 61 18.0 62 20.0 63 18.0 64 14.0 65 10.5 66 7.0 67 5.0 68 4.5 69 3.5 70 1.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 5662.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.681737901801483 #Duplication Level Percentage of deduplicated Percentage of total 1 73.01451750640479 15.100671140939598 2 13.407344150298888 5.54574355351466 3 5.038428693424423 3.126103850229601 4 2.220324508966695 1.8368067820558105 5 0.9393680614859093 0.9713882020487461 6 0.8539709649871904 1.0596962204168139 7 0.1707941929974381 0.24726245143058992 8 0.6831767719897524 1.130342635111268 9 0.3415883859948762 0.6358177322500883 >10 2.6473099914602902 9.625574002119393 >50 0.25619128949615716 3.973860826563052 >100 0.3415883859948762 18.950900741787354 >500 0.0 0.0 >1k 0.08539709649871904 37.795831861533024 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 2140 37.795831861533024 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 436 7.700459201695514 Illumina PCR Primer Index 9 (95% over 22bp) TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 284 5.015895443306253 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 229 4.044507241257507 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT 124 2.190038855528082 No Hit CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 81 1.4305898975626987 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT 80 1.412928293889085 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT 64 1.130342635111268 No Hit CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 50 0.8830801836806783 No Hit TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT 48 0.8477569763334512 Illumina PCR Primer Index 9 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCG 28 0.49452490286117984 No Hit CTTATACACATCTCCGAGCCCACGCGACGTTAACAGATCTCGTATGCCGT 25 0.44154009184033916 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCG 23 0.40621688449311194 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCTT 20 0.35323207347227126 No Hit ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT 20 0.35323207347227126 Illumina PCR Primer Index 9 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG 20 0.35323207347227126 No Hit CTTATACACATCTCCGAGCCCACGAGCCGTTAACAGATCTCGTATGCCGT 18 0.31790886612504415 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 18 0.31790886612504415 No Hit CTTATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGT 17 0.30024726245143063 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACATCGTATGCCGT 17 0.30024726245143063 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC 17 0.30024726245143063 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT 16 0.282585658777817 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT 16 0.282585658777817 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGT 16 0.282585658777817 No Hit TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 14 0.24726245143058992 No Hit CTTATACACATCTCCGAGCCCACGAGAAGTTAACAGATCTCGTATGCCGT 14 0.24726245143058992 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGTCTT 14 0.24726245143058992 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT 13 0.2296008477569763 No Hit ATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTGCTT 12 0.2119392440833628 Illumina PCR Primer Index 9 (96% over 28bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACCGATCTCGTATGCCGT 12 0.2119392440833628 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT 12 0.2119392440833628 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATTTCGTATGCCGT 11 0.1942776404097492 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCC 11 0.1942776404097492 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTAACAGATCTCGTATGCCGT 11 0.1942776404097492 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT 11 0.1942776404097492 No Hit CTTATACACATCTCCGCGCCCACGAGACGTTAACAGATCTCGTATGCCGT 11 0.1942776404097492 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAAATCGTATGCCGT 10 0.17661603673613563 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 10 0.17661603673613563 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT 10 0.17661603673613563 No Hit CTTATACACATCTCCGAGCCCAAGAGACGTTAACAGATCTCGTATGCCGT 9 0.15895443306252208 No Hit CTTATACACATATCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 9 0.15895443306252208 No Hit CTTAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 9 0.15895443306252208 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTAGTATGCCGT 9 0.15895443306252208 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCACAGATCTCGTATGCCGT 8 0.1412928293889085 No Hit CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTA 8 0.1412928293889085 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTCTGCCGT 8 0.1412928293889085 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 8 0.1412928293889085 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCACGTATGCCGT 8 0.1412928293889085 No Hit CTTATACACATCTACGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 8 0.1412928293889085 No Hit CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA 8 0.1412928293889085 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCC 8 0.1412928293889085 No Hit CTTATACACATCTCCGAGCCCACGTGACGTTAACAGATCTCGTATGCCGT 7 0.12363122571529496 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCCCGTATGCCGT 7 0.12363122571529496 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAAATCGTATGCCG 6 0.1059696220416814 No Hit ATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGTCTT 6 0.1059696220416814 Illumina PCR Primer Index 9 (95% over 22bp) ATACACATCTCCGAGCCCACGAGAAGTTAACAGATCTCGTATGCCGTCTT 6 0.1059696220416814 TruSeq Adapter, Index 9 (95% over 22bp) ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTCTGCCGTCTT 6 0.1059696220416814 No Hit GTATAATGTTTACATATTTGTGAAATAATGTTTTATACTGTTTATGGATA 6 0.1059696220416814 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCAACAGATCTCGTATGCCGT 6 0.1059696220416814 No Hit AGATTAATTAGTTCAAAAATCAAACAGCTAATGAGTAAAAGTGCCTGGAT 6 0.1059696220416814 No Hit GCTGGACAGGGTCTTTTCGATGAGTTCCCGCAGCGCAAAGCTTGATGGGA 6 0.1059696220416814 No Hit CTTATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT 6 0.1059696220416814 No Hit TCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 6 0.1059696220416814 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.15895443306252208 0.0 2 0.0 0.0 0.0 0.15895443306252208 0.0 3 0.0 0.0 0.0 0.15895443306252208 0.0 4 0.0 0.0 0.0 0.15895443306252208 0.0 5 0.0 0.0 0.0 0.15895443306252208 0.0 6 0.0 0.0 0.0 0.15895443306252208 0.0 7 0.0 0.0 0.0 0.15895443306252208 0.0 8 0.0 0.0 0.0 0.15895443306252208 0.0 9 0.0 0.0 0.0 0.15895443306252208 0.0 10 0.0 0.0 0.0 0.15895443306252208 0.0 11 0.0 0.0 0.0 0.2472624514305899 0.0 12 0.0 0.0 0.0 0.3002472624514306 0.0 13 0.0 0.0 0.0 0.31790886612504415 0.0 14 0.0 0.0 0.0 0.31790886612504415 0.0 15 0.0 0.0 0.0 0.33557046979865773 0.0 16 0.0 0.0 0.0 0.33557046979865773 0.0 17 0.0 0.0 0.0 0.33557046979865773 0.0 18 0.0 0.0 0.0 0.5475097138820205 0.0 19 0.0 0.0 0.0 0.7417873542917697 0.0 20 0.0 0.0 0.0 0.7771105616389968 0.0 21 0.0 0.0 0.0 0.7771105616389968 0.0 22 0.0 0.0 0.0 0.7771105616389968 0.0 23 0.0 0.0 0.0 0.7771105616389968 0.0 24 0.0 0.0 0.0 0.7771105616389968 0.0 25 0.0 0.0 0.0 0.7947721653126104 0.0 26 0.0 0.0 0.0 0.7947721653126104 0.0 27 0.0 0.0 0.0 0.7947721653126104 0.0 28 0.0 0.0 0.0 0.847756976333451 0.0 29 0.0 0.0 0.0 0.9360649947015189 0.0 30 0.0 0.0 0.0 0.9360649947015189 0.0 31 0.0 0.0 0.0 0.971388202048746 0.0 32 0.0 0.0 0.0 1.0243730130695867 0.0 33 0.0 0.0 0.0 1.1126810314376545 0.0 34 0.0 0.0 0.0 1.1833274461321088 0.0 35 0.0 0.0 0.0 1.2009890498057223 0.0 36 0.0 0.0 0.0 1.342281879194631 0.0 37 0.0 0.0 0.0 1.5718827269516071 0.0 38 0.0 0.0 0.0 1.7131755563405158 0.0 39 0.0 0.0 0.0 1.8544683857294242 0.0 40 0.0 0.0 0.0 2.1193924408336278 0.0 41 0.0 0.0 0.0 2.472624514305899 0.0 42 0.0 0.0 0.0 2.7728717767573294 0.0 43 0.0 0.0 0.0 3.0554574355351467 0.0 44 0.0 0.0 0.0 3.126103850229601 0.0 45 0.0 0.0 0.0 3.408689509007418 0.0 46 0.0 0.0 0.0 3.7089367714588484 0.0 47 0.0 0.0 0.0 3.885552808194984 0.0 48 0.0 0.0 0.0 4.291769692688096 0.0 49 0.0 0.0 0.0 4.468385729424232 0.0 50 0.0 0.0 0.0 4.85694101024373 0.0 51 0.0 0.0 0.0 5.1395266690215475 0.0 52 0.0 0.0 0.0 5.40445072412575 0.0 53 0.0 0.0 0.0 5.5987283645355 0.0 54 0.0 0.0 0.0 5.793006004945249 0.0 55 0.0 0.0 0.0 5.8636524196397035 0.0 56 0.0 0.0 0.0 5.951960438007771 0.0 57 0.0 0.0 0.0 6.022606852702225 0.0 58 0.0 0.0 0.0 6.075591663723066 0.0 59 0.0 0.0 0.0 6.181561285764747 0.0 60 0.0 0.0 0.0 6.2875309078064285 0.0 61 0.0 0.0 0.0 6.499470151889792 0.0 62 0.0 0.0 0.0 6.5877781702578595 0.0 63 0.0 0.0 0.0 6.605439773931473 0.0 64 0.0 0.0 0.0 6.676086188625927 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGATA 15 0.0021117597 70.00001 33 GATATCG 15 0.0021117597 70.00001 36 CAGATAT 15 0.0021117597 70.00001 34 TTGAACA 15 0.0021117597 70.00001 58 CTTGAAC 15 0.0021117597 70.00001 57 TGAACAA 15 0.0021117597 70.00001 59 ATATCGT 15 0.0021117597 70.00001 37 AGATATC 15 0.0021117597 70.00001 35 CTTTACA 25 2.4919646E-6 70.0 1 TATCGTA 20 7.266711E-5 70.0 38 TTACACA 25 2.4919646E-6 70.0 3 ATCGTAT 25 2.4919646E-6 70.0 39 TTTACAC 25 2.4919646E-6 70.0 2 ACTCGTA 25 2.1925599E-4 55.999996 38 AGAACTC 25 2.1925599E-4 55.999996 35 AACTCGT 25 2.1925599E-4 55.999996 37 GAACTCG 25 2.1925599E-4 55.999996 36 CAGAACT 25 2.1925599E-4 55.999996 34 TTATACA 425 0.0 53.52941 2 CTTATAC 420 0.0 53.333332 1 >>END_MODULE