FastQCFastQC Report
Wed 25 May 2016
SRR1780659_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780659_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56594
Sequences flagged as poor quality0
Sequence length50
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATC1180.20850266812736332No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG810.1431247128670884No Hit
CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA590.10425133406368166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAAC254.413759E-543.9584432
TTTACAC501.3114914E-939.56262
CTTTACA404.0345185E-738.4976771
CTTATAA301.285954E-436.6644551
CGAAACT353.1806197E-431.42667625
GGGAACC353.1806197E-431.4266761
GATACAC353.1971984E-431.398893
GGTGGTC502.326431E-630.7709144
TCTTTAT300.005701056729.3315641
TCGTTGG454.028051E-529.30562842
AAACACA300.00572579829.3056284
GTGCCGA406.9154415E-427.52269718
GGGATAA406.951401E-427.4983429
ACGAAAC508.1580845E-526.4217924
CTATACA609.544561E-625.6651171
CCGTGTA450.001375164724.4646234
GGATAAG450.001382291724.44296830
AGCTCGG551.5776674E-423.97733342
GAGCTCG651.787101E-523.6699341
CGGTGGT651.787101E-523.6699343
Produced by FastQC (version 0.11.5)