FastQCFastQC Report
Wed 25 May 2016
SRR1780659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56594
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT3109854.9492879103792No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT51009.011555995335195RNA PCR Primer, Index 14 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG49068.668763473159698No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC33755.963529702795349No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC8881.5690709262465987No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT7401.307559105205499No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC5250.9276601759903876No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC2850.5035869526804961No Hit
ACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCT2140.3781319574513199RNA PCR Primer, Index 14 (95% over 23bp)
ATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCTT2090.3692970986323639RNA PCR Primer, Index 27 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCT1750.3092200586634626RNA PCR Primer, Index 14 (96% over 26bp)
TCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCTTG1660.29331731278934164RNA PCR Primer, Index 14 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1640.2897833692617592No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1250.22087147047390182No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTA1140.20143478107219848No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1040.1837650634342863No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCATCTCGTATGCCGTC1020.1802311199067039No Hit
CTTAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC960.1696292893239566No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC920.16256140226879173No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG800.14135774110329716No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGC610.1077852775912641No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA580.10248436229989046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT207.7930756E-444.0000041
CTTTATA351.4137004E-744.02
CTTTACA850.044.01
TTTACAC850.044.02
TTTATAC351.4137004E-744.03
TCTTTAT404.0326995E-738.5000041
CCGTCTT6050.038.1818244
TAATACA357.168179E-637.7142874
TTAATAC357.168179E-637.7142873
TTACACA1050.035.619053
CTTAATA250.002333938835.22
ATGCCGT40550.034.2342844
AATACAC401.7897342E-533.05
CTTATAC42600.032.7934271
TTATACA42850.032.6020972
GCCGTCT7200.032.08333243
CTATACA551.3348108E-732.01
TATACAC44300.031.584653
CTCTTAT5100.030.6274491
TATGCCG46450.029.79117443
Produced by FastQC (version 0.11.5)