##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780658_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6412 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.529008109794137 23.0 12.0 31.0 12.0 33.0 2 19.28930131004367 12.0 11.0 27.0 11.0 34.0 3 20.35683094198378 21.0 11.0 31.0 11.0 34.0 4 19.518714909544602 21.0 11.0 31.0 11.0 34.0 5 19.93621334996881 21.0 11.0 31.0 11.0 34.0 6 20.196974422956956 11.0 11.0 28.0 11.0 37.0 7 22.05723643169058 21.0 11.0 33.0 11.0 37.0 8 21.382252027448533 21.0 11.0 31.0 11.0 36.0 9 22.49422956955708 21.0 12.0 34.0 12.0 37.0 10-11 21.26325639426076 21.0 11.0 31.0 11.0 37.0 12-13 21.28828758577667 21.0 11.0 31.0 11.0 36.5 14-15 21.227542108546473 21.0 11.0 31.0 11.0 36.5 16-17 20.682626325639426 21.0 11.0 31.0 11.0 36.0 18-19 21.086244541484717 21.0 11.0 31.0 11.0 36.0 20-21 21.13724266999376 21.0 11.0 31.0 11.0 36.0 22-23 20.661883967560826 21.0 11.0 29.5 10.5 36.5 24-25 20.79881472239551 21.0 11.0 31.0 10.5 36.0 26-27 20.480505302557702 21.0 11.0 31.0 11.0 36.0 28-29 19.92732376793512 11.0 11.0 27.5 11.0 36.0 30-31 20.058562071116654 20.0 11.0 27.0 11.0 36.0 32-33 20.32805676855895 21.0 11.0 27.5 11.0 36.0 34-35 20.694167186525263 21.0 11.0 31.0 11.0 36.0 36-37 20.416172800998126 21.0 11.0 27.0 11.0 36.0 38-39 19.602854023705554 11.0 11.0 27.0 11.0 35.0 40-41 19.845056144728634 16.0 11.0 27.0 11.0 34.5 42-43 20.318543356207112 21.0 11.0 27.0 11.0 35.5 44-45 19.803961322520273 15.0 11.0 27.0 11.0 34.5 46-47 20.048346849656895 16.0 11.0 27.0 11.0 35.0 48-49 20.21935433562071 21.0 11.0 27.0 11.0 34.5 50-51 20.549984404242046 21.0 11.0 27.5 11.0 36.0 52-53 20.566281971303805 21.0 11.0 29.0 11.0 36.0 54-55 20.5630068621335 21.0 11.0 29.0 11.0 35.0 56-57 19.77791640673737 16.0 11.0 27.0 11.0 34.0 58-59 20.047411104179666 16.0 11.0 27.0 11.0 34.0 60-61 20.044993761696816 20.0 11.0 27.0 11.0 34.0 62-63 19.660636306924516 15.0 11.0 26.5 11.0 34.0 64-65 19.859326263256396 15.5 11.0 26.5 11.0 34.0 66-67 20.365096693699314 20.0 11.0 28.5 11.0 34.0 68-69 19.51910480349345 19.0 11.0 26.5 11.0 34.0 70-71 19.959217092950716 19.0 11.0 27.5 11.0 34.0 72-73 19.171007485963816 11.0 11.0 26.0 11.0 34.0 74-75 19.69065814098565 19.0 11.0 26.0 11.0 34.0 76 19.584996880848408 19.0 11.0 26.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1801797200454907 1101 2 0.03256299853171285 1101 3 -3.3964737844850283 1101 4 -1.6506144901574267 1101 5 -3.4027925008473296 1101 6 -0.9100187178391081 1101 7 -3.524540496596263 1101 8 -2.1371878003181983 1101 9 -4.816103323355442 1101 10-11 -2.3013671964905207 1101 12-13 -2.437937048750051 1101 14-15 -3.1802690723539655 1101 16-17 -1.4891831191616305 1101 18-19 -2.2867389147366097 1101 20-21 -2.7893033208802294 1101 22-23 -3.6171074505017167 1101 24-25 -1.3523109515002893 1101 26-27 -1.2598600694224587 1101 28-29 -4.106747649178811 1101 30-31 -0.5534127124414532 1101 32-33 -3.738497243730862 1101 34-35 -0.49617939872033645 1101 36-37 -3.6005471461486174 1101 38-39 0.10131382663513122 1101 40-41 -1.5286661902022658 1101 42-43 -0.950005822611022 1101 44-45 -0.8676329737174839 1101 46-47 -3.868553993894146 1101 48-49 -2.760050283834804 1101 50-51 -3.4967191583514747 1101 52-53 1.7747221812777099 1101 54-55 0.15891751883940586 1101 56-57 0.26883391399034196 1101 58-59 -1.4291049000765774 1101 60-61 -1.9283175577003 1101 62-63 0.12422847789782665 1101 64-65 -3.5806321979375824 1101 66-67 -1.4818692138682614 1101 68-69 -1.8114792707111853 1101 70-71 -1.9896011794679715 1101 72-73 1.072336645982162 1101 74-75 0.7089188527579644 1101 76 -0.7209765196556397 1102 1 0.9154738376925486 1102 2 2.6465335867670063 1102 3 3.092496803750265 1102 4 2.29791492160728 1102 5 1.3177957344467863 1102 6 1.2921871645138339 1102 7 2.6666359739919727 1102 8 5.017223964387686 1102 9 3.8677202060563225 1102 10-11 2.7942210388035953 1102 12-13 0.5547100100734781 1102 14-15 4.44840739823427 1102 16-17 1.247949233779547 1102 18-19 3.2941434382045642 1102 20-21 4.523196679119771 1102 22-23 0.9398778436159283 1102 24-25 3.0557772837938266 1102 26-27 0.7713899305775413 1102 28-29 2.4704582331741314 1102 30-31 3.922690228735018 1102 32-33 1.199002756269138 1102 34-35 4.206026483632606 1102 36-37 1.6512910891454986 1102 38-39 0.3384461795763052 1102 40-41 4.337142633327147 1102 42-43 2.879038295036036 1102 44-45 -0.03858885607042595 1102 46-47 3.8244607119882055 1102 48-49 3.7638467749887248 1102 50-51 -0.01326327599853272 1102 52-53 2.392369240101239 1102 54-55 2.883182224721761 1102 56-57 1.980230972813871 1102 58-59 1.863174511688129 1102 60-61 1.374991265829113 1102 62-63 1.5047431837801781 1102 64-65 3.7171619197094756 1102 66-67 0.2883513743670356 1102 68-69 0.9054324939946987 1102 70-71 -0.8407041206444426 1102 72-73 3.916086645982162 1102 74-75 -0.1274782060655646 1102 76 0.09519995093259581 1103 1 1.5733685745346548 1103 2 1.9458756920301639 1103 3 0.20104943532921382 1103 4 3.0150201847651736 1103 5 1.2783220502362589 1103 6 2.486266111882255 1103 7 0.15347807925513024 1103 8 1.1816976485982131 1103 9 1.8216675744773738 1103 10-11 -1.8834105401437746 1103 12-13 0.10076264165242321 1103 14-15 -0.0022504965025724744 1103 16-17 1.7956466022005984 1103 18-19 0.15598554346772175 1103 20-21 1.1054335212250344 1103 22-23 4.944812054142243 1103 24-25 -0.14488061094301585 1103 26-27 1.7664557200512263 1103 28-29 0.7369056015951827 1103 30-31 0.5131507550508054 1103 32-33 -0.2911288226782318 1103 34-35 2.479052799422078 1103 36-37 3.3437252996718136 1103 38-39 -0.7191196098973798 1103 40-41 -0.013186314041274017 1103 42-43 1.6800251371412998 1103 44-45 -1.0007599087020047 1103 46-47 0.3326843961987329 1103 48-49 -0.9318769092218027 1103 50-51 0.7679867240014673 1103 52-53 3.892369240101239 1103 54-55 -1.2451072489624515 1103 56-57 0.07069149912965855 1103 58-59 0.7102139853723415 1103 60-61 0.4292675816185856 1103 62-63 1.562308973253863 1103 64-65 0.5757145512884208 1103 66-67 -0.489609151948752 1103 68-69 -2.624172769163195 1103 70-71 1.312256405671345 1103 72-73 -0.4013475645441531 1103 74-75 -0.5666229429076672 1103 76 2.378094687774702 1104 1 0.41547383769254864 1104 2 -2.5909664132329944 1104 3 -2.5200031962497356 1104 4 0.3229149216072784 1104 5 0.36779573444678704 1104 6 -0.19531283548616685 1104 7 -2.3333640260080273 1104 8 -0.5577760356123136 1104 9 2.142720206056321 1104 10-11 1.1192210388035946 1104 12-13 -0.5702899899265219 1104 14-15 -2.6640926017657307 1104 16-17 -0.8833007662204544 1104 18-19 -2.9558565617954358 1104 20-21 -2.71430332088023 1104 22-23 -1.4538721563840689 1104 24-25 -0.4817227162061748 1104 26-27 -0.7098600694224615 1104 28-29 -0.1670417668258679 1104 30-31 -0.6960597712649808 1104 32-33 -0.4134972437308626 1104 34-35 0.34352648363260485 1104 36-37 0.3700410891454986 1104 38-39 -0.7928038204236962 1104 40-41 -0.5316073666728514 1104 42-43 0.010288295036037454 1104 44-45 -2.1573388560704245 1104 46-47 -1.4067892880117974 1104 48-49 2.8825967749887234 1104 50-51 -1.8820132759985313 1104 52-53 -2.857630759898761 1104 54-55 2.0956822247217595 1104 56-57 -3.2135190271861305 1104 58-59 1.7444245116881305 1104 60-61 -0.6062587341708863 1104 62-63 -0.7390068162198205 1104 64-65 -3.395338080290525 1104 66-67 -0.9803986256329615 1104 68-69 -0.9320675060052999 1104 70-71 1.4280458793555582 1104 72-73 -2.1526633540178395 1104 74-75 -1.133728206065566 1104 76 -2.4298000490674028 1105 1 -1.5845261623074514 1105 2 -4.527808518496151 1105 3 -1.69368740677605 1105 4 -2.142874552076929 1105 5 -3.669046370816371 1105 6 -1.513733888117745 1105 7 -0.5833640260080273 1105 8 0.3395923854403158 1105 9 -0.44149032025946866 1105 10-11 -0.17288422435430206 1105 12-13 0.1797100100734781 1105 14-15 0.05038108244479744 1105 16-17 -1.1517218188520317 1105 18-19 1.0244065960993005 1105 20-21 2.1843808896460857 1105 22-23 0.44481205414224334 1105 24-25 -2.9080385056798583 1105 26-27 -1.1282811220540374 1105 28-29 -1.5788838720890261 1105 30-31 1.5131507550508054 1105 32-33 -1.0806025068887593 1105 34-35 2.1895791152115507 1105 36-37 0.8437252996718136 1105 38-39 -1.877014346739486 1105 40-41 -2.697396840357065 1105 42-43 -2.3726064418060666 1105 44-45 -1.2902335929125321 1105 46-47 1.9379475540934656 1105 48-49 -1.3792453302744327 1105 50-51 -1.2583290654722177 1105 52-53 -1.4234202335829735 1105 54-55 0.07068222472176089 1105 56-57 -4.034571658765076 1105 58-59 -4.131891277785556 1105 60-61 0.08716231846069178 1105 62-63 -2.595585763588243 1105 64-65 -1.555864396079997 1105 66-67 -1.2264512572119095 1105 68-69 -2.439962242847404 1105 70-71 -1.16142780485497 1105 72-73 -2.348715985596783 1105 74-75 0.5912717939344354 1105 76 -0.7271684701200343 1106 1 -2.0551143976015673 1106 2 0.26785711617877084 1106 3 3.309408568456149 1106 4 0.7611502157249284 1106 5 0.4207369109173733 1106 6 0.5017459880432469 1106 7 2.0048712681096212 1106 8 -1.1960113297299628 1106 9 3.0074260884092645 1106 10-11 0.25745633292124026 1106 12-13 0.2091217747793621 1106 14-15 -0.9155631900010235 1106 16-17 0.304934527897192 1106 18-19 2.272084614675151 1106 20-21 1.5930496202962416 1106 22-23 0.7064219612629863 1106 24-25 -1.8523109515002893 1106 26-27 -2.6128012458930456 1106 28-29 -0.1949829432964556 1106 30-31 1.8877637581467823 1106 32-33 -1.0620266554955649 1106 34-35 -0.4667676340144524 1106 36-37 -2.7770177343839144 1106 38-39 -1.8398626439531078 1106 40-41 0.5007455745036182 1106 42-43 1.3441118244478005 1106 44-45 0.13236702628251606 1106 46-47 2.10203424139997 1106 48-49 -1.4659326367759782 1106 50-51 -1.7026015112926487 1106 52-53 0.8923692401012389 1106 54-55 -1.399906010572355 1106 56-57 -2.084107262480245 1106 58-59 2.865012746982245 1106 60-61 -0.634199910641474 1106 62-63 0.30069906613312014 1106 64-65 2.4487795667683017 1106 66-67 1.3416601978964486 1106 68-69 -1.5173616236523593 1106 70-71 1.7162811734732024 1106 72-73 -0.36883982460607356 1106 74-75 -1.467551735477329 1106 76 -3.015094166714462 1107 1 -1.536907114688404 1107 2 0.30427168200510124 1107 3 -1.6961936724402094 1107 4 1.5538673025596594 1107 5 -0.07506140841035602 1107 6 0.188020497847166 1107 7 -3.440506883150885 1107 8 0.34460491676863825 1107 9 1.29033925367537 1107 10-11 1.45493532451788 1107 12-13 0.9416147719782408 1107 14-15 -0.7867116493847774 1107 16-17 1.7881278052081164 1107 18-19 1.4667624858236117 1107 20-21 0.9726014410245298 1107 22-23 -3.3860150135269294 1107 24-25 0.9278010933176368 1107 26-27 0.31156850200611075 1107 28-29 1.015101090316989 1107 30-31 1.6146545144493025 1107 32-33 1.7853122800786636 1107 34-35 -1.3267116116054893 1107 36-37 2.512898232002641 1107 38-39 3.0452914176715424 1107 40-41 1.4993450142795268 1107 42-43 1.9519549617027003 1107 44-45 1.2009944772629062 1107 46-47 0.2813059500834427 1107 48-49 0.6195015368934875 1107 50-51 0.05370100971575198 1107 52-53 1.3447501924821914 1107 54-55 0.7135393675789032 1107 56-57 2.14838573471863 1107 58-59 1.5134721307357495 1107 60-61 2.7425507896386385 1107 62-63 4.464564612351609 1107 64-65 1.5118047768523297 1107 66-67 0.7898394696051305 1107 68-69 -1.0915913155291115 1107 70-71 0.08042683173651 1107 72-73 2.1199556936012094 1107 74-75 1.972224174886815 1107 76 -1.5893238585912144 1108 1 -2.632145209926499 1108 2 -2.600490222756804 1108 3 0.7323777561312177 1108 4 -0.11279936410700486 1108 5 -1.7417280750770239 1108 6 -1.2881699783433085 1108 7 -2.8214592641032645 1108 8 -2.8458712737075516 1108 9 -1.1858512225151046 1108 10-11 -0.497445627863069 1108 12-13 1.6082814386449087 1108 14-15 -0.8581402208133504 1108 16-17 -2.1642531471728326 1108 18-19 -1.5332375141763883 1108 20-21 -2.0035890351659447 1108 22-23 -4.7669673944793125 1108 24-25 -2.572198906682363 1108 26-27 -0.2122410218034112 1108 28-29 -1.6277560525401533 1108 30-31 -3.9567740569792687 1108 32-33 -1.9527829580165772 1108 34-35 -2.4695687544626352 1108 36-37 -2.9632922441878335 1108 38-39 -1.502327629947505 1108 40-41 -1.3577978428633273 1108 42-43 -1.2861402763925334 1108 44-45 0.10575638202481485 1108 46-47 0.2574964262739208 1108 48-49 -2.8804984631065125 1108 50-51 1.5060819620967045 1108 52-53 -2.2504879027559035 1108 54-55 -4.334079680040144 1108 56-57 -1.8039952176623189 1108 58-59 -2.843670726407108 1108 60-61 -2.376496829408982 1108 62-63 -0.7497211019341066 1108 64-65 -1.8453380802905244 1108 66-67 -1.233970054204395 1108 68-69 -1.6630198869576809 1108 70-71 -2.7290969777873 1108 72-73 -2.9752824016368855 1108 74-75 -0.17063296797032734 1108 76 1.1725809033135484 1109 1 1.8891580482188637 1109 2 -0.3172822027066786 1109 3 -1.2200031962497349 1109 4 -1.3534008678664051 1109 5 1.7520062607625775 1109 6 -2.2505759933809024 1109 7 1.574530710834079 1109 8 0.497487122282422 1109 9 -0.020437688680519983 1109 10-11 -1.962357908564826 1109 12-13 -0.37292156887389183 1109 14-15 -0.8180399701867813 1109 16-17 0.40090976009533463 1109 18-19 -0.02822498284806585 1109 20-21 -1.3682506893012842 1109 22-23 0.865864685721192 1109 24-25 -1.092249031995646 1109 26-27 0.47698203584069887 1109 28-29 1.0000634963320252 1109 30-31 -0.14474398179129722 1109 32-33 2.603608019427032 1109 34-35 -2.5998945689989768 1109 36-37 -0.15627470032818636 1109 38-39 -0.9296459256868523 1109 40-41 -0.19739684035706517 1109 42-43 -0.29365907338501884 1109 44-45 -3.290233592912532 1109 46-47 -1.5883682353802158 1109 48-49 1.3312809855150398 1109 50-51 -1.7846448549459026 1109 52-53 -2.97605181253034 1109 54-55 0.5706822247217609 1109 56-57 -2.2187821850808653 1109 58-59 -1.0003123304171346 1109 60-61 -4.149679786802466 1109 62-63 0.5359931837801781 1109 64-65 -2.4242854487115757 1109 66-67 -3.121188099317177 1109 68-69 -1.8346990849526712 1109 70-71 0.5754143004081875 1109 72-73 -0.08555809085994426 1109 74-75 -1.1455703113287221 1109 76 0.1675683719852259 1110 1 1.7336556558743652 1110 2 0.2999426776760963 1110 3 -0.7200031962497349 1110 4 0.9456421943345532 1110 5 -1.795840629189577 1110 6 2.263778073604744 1110 7 -3.219727662371664 1110 8 1.164951237114959 1110 9 -0.8481888848527674 1110 10-11 -1.353506233923678 1110 12-13 -0.9566536262901586 1110 14-15 1.2836346709615434 1110 16-17 -1.0787553116749997 1110 18-19 0.5100525291136542 1110 20-21 2.5288784973015908 1110 22-23 -0.8697812472931616 1110 24-25 0.48191364743018994 1110 26-27 -0.873496433058822 1110 28-29 0.20341277862867813 1110 30-31 0.9653038650986545 1110 32-33 1.443320938087318 1110 34-35 -0.09510988000375775 1110 36-37 -1.016322547218138 1110 38-39 0.3071961795763052 1110 40-41 -0.06342554849103266 1110 42-43 -2.464711704963964 1110 44-45 2.6403884166568474 1110 46-47 -0.18406201528452115 1110 48-49 -0.5060395886476385 1110 50-51 -1.1183769123621694 1110 52-53 0.6878237855557856 1110 54-55 0.0025004065399407693 1110 56-57 0.13648097281387095 1110 58-59 2.0989699662335823 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0.09471254602222601 2116 72-73 1.072336645982162 2116 74-75 -1.8087282060655632 2116 76 -2.04646671573407 2117 1 2.4154738376925486 2117 2 0.8244182021516231 2117 3 1.395381419134882 2117 4 4.0190687677611265 2117 5 1.5293341959852462 2117 6 2.008533318359987 2117 7 2.2627898201458194 2117 8 0.6229931951569156 2117 9 1.0888740522101692 2117 10-11 -1.984625115042558 2117 12-13 -2.5895207591572884 2117 14-15 -3.27755414022727 2117 16-17 -2.1294546123742997 2117 18-19 -1.0423950233338957 2117 20-21 -0.7123802439571527 2117 22-23 -0.8365644640763783 2117 24-25 -1.0355688700523267 2117 26-27 -0.41370622326861195 2117 28-29 0.6194966947125913 2117 30-31 -3.286444386649599 2117 32-33 -2.238497243730862 2117 34-35 -1.500704285598161 2117 36-37 -2.2453435262391146 2117 38-39 -0.5774192050390816 2117 40-41 -4.381607366672853 2117 42-43 -1.253173243425504 2117 44-45 -0.13233885607042595 2117 46-47 -2.4952508264733346 2117 48-49 -2.5654801480881986 2117 50-51 0.6526021086168505 2117 52-53 -1.7614769137449144 2117 54-55 2.9168360708756076 2117 56-57 1.386480972813871 2117 58-59 1.0098091270727423 2117 60-61 -0.3966433495555002 2117 62-63 -1.1563145085275153 2117 64-65 3.1931234581710157 2117 66-67 2.925370605136269 2117 68-69 1.2948555709177754 2117 70-71 -3.795031043721366 2117 72-73 -1.427663354017838 2117 74-75 1.168194870857512 2117 76 2.5278922586249024 2118 1 0.48214050435921507 2118 2 0.009033586767007051 2118 3 1.7799968037502651 2118 4 -2.627085078392721 2118 5 0.7344624011134542 2118 6 -2.8786461688195004 2118 7 -2.0500306926746923 2118 8 -1.407776035612315 2118 9 -3.2906131272770125 2118 10-11 -2.064112294529739 2118 12-13 -1.2869566565931905 2118 14-15 0.26090739823427 2118 16-17 -0.8499674328871194 2118 18-19 -0.48085656179543435 2118 20-21 -0.5226366542135636 2118 22-23 -0.46220548971740527 2118 24-25 -2.0483893828728412 2118 26-27 0.04013993057753851 2118 28-29 0.6246248998407999 2118 30-31 -1.5377264379316493 2118 32-33 0.7948360896024695 2118 34-35 -2.99814018303406 2118 36-37 -3.696625577521168 2118 38-39 1.3738628462429716 2118 40-41 3.018392633327146 2118 42-43 1.8186216283693675 2118 44-45 2.500994477262907 2118 46-47 2.643210711988207 2118 48-49 2.890930108322056 2118 50-51 -0.9986799426651984 2118 52-53 -0.740964093232094 2118 54-55 -2.5959844419449034 2118 56-57 1.4198143061472024 2118 58-59 4.127757845021463 2118 60-61 -0.5812587341708877 2118 62-63 -0.09734014955315473 2118 64-65 0.15466191970947563 2118 66-67 1.894601374367035 2118 68-69 -1.4487341726719656 2118 70-71 -0.5719541206444418 2118 72-73 -1.4943300206845045 2118 74-75 1.924605127267771 2118 76 -1.7131333824007378 2119 1 -0.7511928289741192 2119 2 -2.2076330798996615 2119 3 1.696663470416933 2119 4 0.6729149216072798 2119 5 -2.3488709322198815 2119 6 -2.061979502152834 2119 7 -3.6666973593413594 2119 8 -6.441109368945648 2119 9 0.9927202060563225 2119 10-11 -2.5807789611964047 2119 12-13 -0.3202899899265219 2119 14-15 0.010907398234270005 2119 16-17 -5.449967432887121 2119 18-19 -0.7475232284621001 2119 20-21 -1.4143033208802294 2119 22-23 -2.987205489717404 2119 24-25 -3.090056049539509 2119 26-27 0.7818065972442092 2119 28-29 -4.758708433492535 2119 30-31 -2.796059771264982 2119 32-33 -3.5718305770641976 2119 34-35 -3.2731401830340623 2119 36-37 -2.7549589108545014 2119 38-39 -0.026137153757030518 2119 40-41 -1.339940700006185 2119 42-43 -2.464711704963964 2119 44-45 0.07599447726290975 2119 46-47 -3.5401226213451267 2119 48-49 1.7325967749887248 2119 50-51 -3.148679942665197 2119 52-53 1.6423692401012389 2119 54-55 -0.8876511086115748 2119 56-57 -2.738519027186129 2119 58-59 -1.2722421549785352 2119 60-61 -3.2395920675042227 2119 62-63 0.24432651711351383 2119 64-65 -1.8453380802905244 2119 66-67 0.6112680410337035 2119 68-69 -2.0320675060053013 2119 70-71 -1.8719541206444426 2119 72-73 -0.38599668735117376 2119 74-75 -3.6587282060655646 2119 76 -2.446466715734072 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 20.0 13 125.0 14 336.0 15 454.0 16 623.0 17 711.0 18 757.0 19 722.0 20 637.0 21 478.0 22 348.0 23 238.0 24 163.0 25 118.0 26 85.0 27 60.0 28 51.0 29 44.0 30 42.0 31 39.0 32 30.0 33 37.0 34 51.0 35 58.0 36 87.0 37 98.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.00376647834275 29.409918392969242 7.925298179535467 54.66101694915254 2 16.905801621958826 24.40736119775421 29.03930131004367 29.647535870243292 3 5.988771054273237 42.451653150343105 21.756082345601996 29.80349344978166 4 17.233312538989395 28.290704928259515 21.490954460386774 32.98502807236432 5 7.002495321272614 34.01434809731753 31.971303805364943 27.011852776044915 6 22.0991890205864 33.156581409856514 12.024329382407984 32.7199001871491 7 7.298814722395508 47.64504054897068 11.618839675608235 33.43730505302558 8 6.238303181534623 38.25639426076107 23.45601996257018 32.04928259513412 9 11.36930754834685 30.31815346225827 14.238927011852775 44.07361197754211 10-11 8.476955470638696 31.342119628792016 26.265304530921004 33.91562036964829 12-13 7.65751715533375 27.822832189644416 24.781659388646286 39.737991266375545 14-15 12.77292576419214 32.11946350592639 17.98190892077355 37.12570180910792 16-17 16.773237679351215 30.567685589519648 18.33281347473487 34.32626325639426 18-19 12.156893325015595 28.66500311915159 17.935121646912037 41.242981908920775 20-21 11.252339363693075 30.02963194011229 18.16126013724267 40.556768558951966 22-23 17.638802245789144 29.148471615720524 16.11821584529008 37.09451029320025 24-25 13.482532751091703 35.07485963817841 15.322832189644418 36.119775421085464 26-27 11.228945726762321 35.75327510917031 19.830006238303184 33.18777292576419 28-29 13.49812850904554 30.70804741110418 23.947286338116033 31.84653774173425 30-31 13.466936993137866 29.772301933873983 25.717404865876482 31.043356207111668 32-33 10.074859638178415 35.03587024329383 23.31565814098565 31.57361197754211 34-35 9.653774173424829 37.4142233312539 19.752027448533998 33.17997504678728 36-37 11.657829070492825 31.963505926388024 21.810667498440424 34.56799750467873 38-39 13.123830318153463 25.849968808484093 27.62788521522146 33.39831565814099 40-41 10.472551466001248 27.84622582657517 24.758265751715534 36.922956955708045 42-43 6.136930754834685 25.54585152838428 27.588895820336866 40.72832189644417 44-45 7.509357454772301 25.99033063006862 31.136930754834687 35.363381160324394 46-47 7.868059887710542 31.035558328134748 27.854023705552088 33.24235807860262 48-49 8.507485963817842 33.873986275732996 21.65470991890206 35.9638178415471 50-51 7.150655021834061 33.65564566437929 18.92545227698066 40.26824703680599 52-53 6.136930754834685 33.265751715533376 21.218028696194636 39.3792888334373 54-55 6.33967560823456 30.302557704304427 21.093262632563942 42.26450405489707 56-57 11.065190268247036 32.595134123518406 19.260761072988146 37.07891453524642 58-59 9.380848409232689 31.21490954460387 18.036494073611976 41.36774797255146 60-61 7.025888958203369 28.555832813474737 20.82813474734872 43.590143480973175 62-63 10.129444791016844 28.93013100436681 19.307548346849657 41.632875857766685 64-65 10.277604491578291 30.513100436681224 14.847161572052403 44.362133499688085 66-67 8.070804741110418 36.47847785402371 13.209606986899564 42.24111041796632 68-69 9.840923268870867 35.917030567685586 14.199937616968183 40.042108546475355 70-71 7.548346849656894 40.51777916406738 15.424204616344353 36.50966936993138 72-73 8.967560823456019 35.99500935745477 19.221771678103554 35.815658140985654 74-75 8.024017467248909 42.50623830318154 14.028384279475983 35.44135995009358 76 5.66126013724267 37.44541484716157 13.256394260761073 43.63693075483469 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 1.0 15 3.0 16 4.5 17 5.0 18 4.5 19 3.5 20 3.5 21 4.0 22 7.0 23 12.5 24 14.5 25 14.0 26 16.0 27 25.0 28 34.0 29 36.0 30 42.5 31 58.5 32 77.5 33 87.0 34 99.5 35 130.0 36 165.5 37 183.0 38 192.0 39 243.5 40 280.5 41 324.5 42 374.0 43 376.0 44 376.5 45 400.5 46 426.0 47 408.5 48 382.0 49 376.5 50 380.0 51 361.0 52 327.5 53 289.5 54 266.0 55 259.5 56 215.0 57 164.0 58 151.0 59 138.0 60 129.0 61 113.0 62 93.0 63 84.0 64 74.5 65 52.0 66 29.0 67 28.0 68 20.0 69 14.5 70 13.0 71 9.0 72 10.5 73 10.5 74 7.0 75 5.0 76 5.5 77 4.0 78 3.0 79 4.0 80 2.5 81 1.0 82 1.5 83 2.0 84 1.5 85 1.0 86 1.5 87 2.0 88 2.5 89 1.5 90 1.0 91 2.5 92 3.0 93 1.5 94 0.5 95 1.0 96 1.0 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6238303181534623 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.007797878976918278 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.28446662507798 #Duplication Level Percentage of deduplicated Percentage of total 1 98.79403364011425 97.0991890205864 2 0.8092668993970167 1.5907673112913288 3 0.31735956839098695 0.9357454772301934 4 0.031735956839098696 0.12476606363069245 5 0.031735956839098696 0.1559575795383656 6 0.015867978419549348 0.09357454772301933 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1091703056768559 0.0 2 0.0 0.0 0.0 0.1091703056768559 0.0 3 0.0 0.0 0.0 0.1091703056768559 0.0 4 0.0 0.0 0.0 0.1091703056768559 0.0 5 0.0 0.0 0.0 0.1091703056768559 0.0 6 0.0 0.0 0.0 0.1091703056768559 0.0 7 0.0 0.0 0.0 0.1091703056768559 0.0 8 0.0 0.0 0.0 0.1091703056768559 0.0 9 0.0 0.0 0.0 0.1091703056768559 0.0 10 0.0 0.0 0.0 0.1091703056768559 0.0 11 0.0 0.0 0.0 0.12476606363069245 0.0 12 0.0 0.0 0.0 0.12476606363069245 0.0 13 0.0 0.0 0.0 0.15595757953836556 0.0 14 0.0 0.0 0.0 0.15595757953836556 0.0 15 0.0 0.0 0.0 0.2183406113537118 0.0 16 0.0 0.0 0.0 0.2183406113537118 0.0 17 0.0 0.0 0.0 0.23393636930754835 0.0 18 0.0 0.0 0.0 0.3587024329382408 0.0 19 0.0 0.0 0.0 0.4990642545227698 0.0 20 0.0 0.0 0.0 0.5458515283842795 0.0 21 0.0 0.0 0.0 0.561447286338116 0.0 22 0.0 0.0 0.0 0.6550218340611353 0.0 23 0.0 0.0 0.0 0.670617592014972 0.0 24 0.0 0.0 0.0 0.7174048658764816 0.0 25 0.0 0.0 0.0 0.7174048658764816 0.0 26 0.0 0.0 0.0 0.7174048658764816 0.0 27 0.0 0.0 0.0 0.7485963817841547 0.0 28 0.0 0.0 0.0 0.810979413599501 0.0 29 0.0 0.0 0.0 0.810979413599501 0.0 30 0.0 0.0 0.0 0.8421709295071741 0.0 31 0.0 0.0 0.0 0.8421709295071741 0.0 32 0.0 0.0 0.0 0.8889582033686837 0.0 33 0.0 0.0 0.0 0.9669369931378665 0.0 34 0.0 0.0 0.0 1.0449157829070492 0.0 35 0.0 0.0 0.0 1.122894572676232 0.0 36 0.0 0.0 0.0 1.2008733624454149 0.0 37 0.0 0.0 0.0 1.372426699937617 0.0 38 0.0 0.0 0.0 1.824703680598877 0.0 39 0.0 0.0 0.0 2.0898315658140985 0.0 40 0.0 0.0 0.0 2.2301933873986277 0.0 41 0.0 0.0 0.0 2.3861509669369934 0.0 42 0.0 0.0 0.0 2.682470368059888 0.0 43 0.0 0.0 0.0 2.8696194635059262 0.0 44 0.0 0.0 0.0 3.165938864628821 0.0 45 0.0 0.0 0.0 3.477854023705552 0.0 46 0.0 0.0 0.0 3.6338116032439176 0.0 47 0.0 0.0 0.0 3.7429819089207736 0.0 48 0.0 0.0 0.0 3.8521522145976292 0.0 49 0.0 0.0 0.0 3.9145352464129757 0.0 50 0.0 0.0 0.0 4.008109794135995 0.0 51 0.0 0.0 0.0 4.148471615720524 0.0 52 0.0 0.0 0.0 4.242046163443543 0.0 53 0.0 0.0 0.0 4.366812227074236 0.0 54 0.0 0.0 0.0 4.538365564566438 0.0 55 0.0 0.0 0.0 4.616344354335621 0.0 56 0.0 0.0 0.0 4.647535870243294 0.0 57 0.0 0.0 0.0 4.75670617592015 0.0 58 0.0 0.0 0.0 4.819089207735496 0.0 59 0.0 0.0 0.0 4.943855271366188 0.0 60 0.0 0.0 0.0 4.990642545227698 0.0 61 0.0 0.0 0.0 5.053025577043044 0.0 62 0.0 0.0 0.0 5.193387398627573 0.0 63 0.0 0.0 0.0 5.318153462258266 0.0 64 0.0 0.0 0.0 5.427323767935122 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE