FastQCFastQC Report
Wed 25 May 2016
SRR1780658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6412
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT259640.4865876481597No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT4126.4254522769806615RNA PCR Primer, Index 42 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG3845.988771054273237No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2353.665003119151591No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1662.588895820336868No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT931.4504054897067997No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT831.294447910168434No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC741.154086088583905No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC580.9045539613225203RNA PCR Primer, Index 32 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT390.6082345601996257RNA PCR Primer, Index 42 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG280.43668122270742354No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC280.43668122270742354No Hit
CTTATACACATCTCCGAGCCCACGCGACATCACTCGATCTCGTATGCCGT270.42108546475358705No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCC250.38989394884591394No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCGT250.38989394884591394No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG220.3431066749844042No Hit
CTTATACACATCTCCGAGCACACGAGACATCACTCGATCTCGTATGCCGT200.3119151590767312No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTT190.29631940112289457No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG190.29631940112289457No Hit
ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT190.29631940112289457RNA PCR Primer, Index 42 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTAGTATGCCGT190.29631940112289457No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT180.280723643169058No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC170.26512788521522146No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT170.26512788521522146No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGTCTT170.26512788521522146No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGT160.2495321272613849No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT130.20274485339987522No Hit
CTTATACACATCTCCGCGCCCACGAGACATCACTCGATCTCGTATGCCGT130.20274485339987522No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC120.18714909544603867No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTCTGCCGT120.18714909544603867No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTT120.18714909544603867No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT120.18714909544603867No Hit
ATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTGCTT120.18714909544603867RNA PCR Primer, Index 32 (96% over 29bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG110.1715533374922021No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCCCGTATGCCGT110.1715533374922021No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC110.1715533374922021RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT110.1715533374922021No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAAATCGTATGCCGT100.1559575795383656No Hit
CTTATACACATATCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT100.1559575795383656No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCACGTATGCCGT100.1559575795383656No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATTTCGTATGCCGT90.140361821584529No Hit
CTTATACACATCTCCGAGCCCAAGAGACATCACTCGATCTCGTATGCCGT90.140361821584529No Hit
CTTATACACATCTCCGAGCCCACGAGACAACACTCGATCTCGTATGCCGT90.140361821584529No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT90.140361821584529No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACACGATCTCGTATGCCGT90.140361821584529No Hit
CTTATACACATCTACGAGCCCACGAGACATCACTCGATCTCGTATGCCGT80.12476606363069245No Hit
CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG80.12476606363069245No Hit
CTTATCACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC80.12476606363069245RNA PCR Primer, Index 32 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAAATCGTATGCCG70.10917030567685589No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACATCGTATGCCGT70.10917030567685589No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT70.10917030567685589No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG70.10917030567685589No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA70.10917030567685589No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGTCTT70.10917030567685589No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCAAA150.002124903570.058
TGCAAAA150.002124903570.059
GCAAAAA150.002124903570.060
TGCTTGC150.002124903570.055
GCTTGCA150.002124903570.056
CTTGCAA150.002124903570.057
GAAAAAC200.00664189652.560
AACACCA200.00664189652.570
CTTATAC5050.051.9801981
TTATACA5100.051.470592
TATACAC5100.050.7843133
TCGATCT4550.050.034
GATCTCG4600.049.4565236
CGATCTC4600.049.4565235
CTCGATC4550.049.2307733
ATCTCGT4700.048.40425537
TCTCGTA4700.048.40425538
ACTCGAT5000.048.332
CTCGTAT5100.048.03921539
GACATCA5400.047.96296326
Produced by FastQC (version 0.11.5)