FastQCFastQC Report
Wed 25 May 2016
SRR1780657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGGCCCGATCGCGGAGCCGGCGCCGCGGGCGCCGTAGCCCAGGTGC11.2048192771084338No Hit
GGCCATGCGCACGCCGCCGATGGGACCGGAGAACGGCAGGCCGGACAGCT11.2048192771084338No Hit
CACCACCCACGTGTGGTCCGGCCACGCGGCGCGGGCGTCCATCACGCCCT11.2048192771084338No Hit
ATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGA11.2048192771084338No Hit
GTTTTGGAGGGTAACAGCTATCCATTGGTCTTATGCCCCAAAAATTTTGT11.2048192771084338No Hit
ATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTG11.2048192771084338No Hit
GTTAGGTACTGTTTGCATTAGTAACTTAAAGGCCCATAGGGTCTTCTCGT11.2048192771084338No Hit
NGAGAGAATCTTGAAACCAGCAAGAGAACAGCAACTGCAGCTGACAAGGG11.2048192771084338No Hit
GCCCAGGCCCTCGCCCACCCCACGTGGGACATGGGCCTTTCTCTTATACA11.2048192771084338No Hit
GTGCCAGACGATCCGCCCCTTCGGTCGCGGCGCCCTGCGCCTGTCTCTTA11.2048192771084338No Hit
GCGATAGTGTTATTGGTTCTATGCATAGACGGGGGGATGTGATCTTGATC11.2048192771084338No Hit
CTCAATCATATACCAAATCTCTCCCTCACTAGACGTAAGCCTTCTCCTCA11.2048192771084338No Hit
AAATAAGAGACAGCTGAACCTTCGTGGAGCCATTCATACCTGTCTCTTAT11.2048192771084338No Hit
ACTTTATACAAATTGGCCTCTGAGAATAAAACTTTCACATCATTTGCTTT11.2048192771084338No Hit
GAATGGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAG11.2048192771084338No Hit
CGGCCGTGCCGCGCCCCGAGTCGGCCCCGCGCGACGACGCGTCGGGCGGA11.2048192771084338No Hit
GAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAGTGTGGCGAGTC11.2048192771084338No Hit
ATAACATTCACAGCCACAGAACTAATCATATTTTATATCTTCTTCGAAAC11.2048192771084338No Hit
TCCCCCACATCTGGGTGAAGGCGATCAGCGAGGCGCACGGCACGATCCTC11.2048192771084338No Hit
CCAGTGGGCCGTCGTCGGGGCGTTTCAGGCCCAGGACCCCTTCCAGGACC11.2048192771084338No Hit
ATCCTCGACACCGTGGTGATGATAGGCACCTTCGGGCAGACGTTCGGGGC11.2048192771084338No Hit
GTACGAGGCCGGCGCCGCGCTCGAACGCCTCGTGGCCGAGCGCCGGGGCG11.2048192771084338No Hit
GGCTTGCAGGCTCTTTTGCAACAACGACATGAAGTCTGTCTCTTATACAC11.2048192771084338No Hit
AAGGCCGGGTCCTCGGTGACGACGGCGACGCCCCCCCCCGTCGCGACCAG11.2048192771084338No Hit
AAGCTAATGTGATATTGCCATCGATGCATCAATTACAGCTCCCCTTCGGT11.2048192771084338No Hit
ACCCAGCTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAATATTCAT11.2048192771084338No Hit
GACACCAACATTGGACTGCAAAAAACGAATTATGGAATATAACTGTAGAA11.2048192771084338No Hit
AACAACGGCGGAGCCTCCTTCCCCACGCCCATGGTTCCCCCTCTTTTCGT11.2048192771084338No Hit
GGCTGGAGTGCAGTGATGCAATCTCAGCTCATTGTAACCTCTGTCTCCTG11.2048192771084338No Hit
GCCCCGGCCGGCGCAACAGCCCCGGCGCCGGGGGCGGGGGTGGCCCAGAC11.2048192771084338No Hit
GGCGTGGACCGGGCGCAGATCTCGATCACACCGTCCTCCCGGTGTGGATC11.2048192771084338No Hit
GCCTACTGAGTCGCCTCCTCCGAATCATTGGACTAGTTCTGTCCCAATGT11.2048192771084338No Hit
GGCCCTGGCGGAGCGCCTGCAGGCCCCGATCGTCTACGCGCTGCGCGGCA11.2048192771084338No Hit
TTCTTAGACTCTGACATGCTTAAATGAGGAAGTGCTGCATACAGCTGTCA11.2048192771084338No Hit
GACCCGGGTGGAGGCGAGCCCGGCTGCTGGACGCGGACGCCACCCGGACC11.2048192771084338No Hit
GTGGGGGGTGTCTTTGGGGTTTGGTTGGTTCGGGGTATGGGGTTAGCCTG11.2048192771084338No Hit
ATACTGGACGAGGCCGCCAAGCGCGAGTCCGTGATGTCCTGTCTCTTATA11.2048192771084338No Hit
GCTTTATGTACTATGTACTGTTAAAGATGGGTAGGTTTGTTGGTATCCTA11.2048192771084338No Hit
CCGAGGCCCTCCTTCGCCCGAACGGGGGCGCCGCGGTTGATGGCCACCCG11.2048192771084338No Hit
GTTCGGCGCCGGCGGCGCGGACGTCCTCGGCGGGATCGGGGTTGCGCTGT11.2048192771084338No Hit
CGGACCACTCGCTCGGCTGCGCGTACTCCCCGCCCATGAACAGCAGCTGC11.2048192771084338No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG11.2048192771084338No Hit
GGGGAGCGGGAGATTGACTGGTGGGTGGGTGGTTTGGTTGCGGTGTAATG11.2048192771084338No Hit
ATTACATTCCCTTCCATTCCACGCGGAATGATTGATTCCATTCCATTCCA11.2048192771084338No Hit
AGAGGAGTCCGTAAAGAGGCATCTTTACTATAAAAGCTATTGTGTAAGCT11.2048192771084338No Hit
CGGGTGCACCTTCGCCGGGTCCGCGAACACCCCGGACTCGCGGTCCGCCA11.2048192771084338No Hit
CCATGGTGTCGATCAGCCGGACGGGCGGCACGTGCGCGGCCACGAGGCTG11.2048192771084338No Hit
CCGCTCCTCCGGCAAGGACCTGGGCGTCGAGCGCTGCTTCGACCTGCTGG11.2048192771084338No Hit
TTGGTGTATAGCATAGCCTTTTTGAGCCTTTCTTCAGCTTCATGATGCTG11.2048192771084338No Hit
CTCGAGGGCCTCGACGCCGTCGATCTCGGTGAGCAGCTGCGGGACGATCG11.2048192771084338No Hit
GTCGTAGTCGTGGTGGAACTCGAAGTAGAAGCGCAGCGCCTGTCTCTTAT11.2048192771084338No Hit
CTCCTGCCCGCGCCGTACCTCATGCTTCTGACCAGTGTATCCCTCCTGTT11.2048192771084338No Hit
TAAGTACAGGGTCCTTAGCCACTAAAGGGGCTTCCCCTGATAACCGCACC11.2048192771084338No Hit
GGTTAGCGATGGAGGCAGGATTGGGGCTGTGGGTGAAAGAGTATGATGGG11.2048192771084338No Hit
CTATACACATCTGACGCTGCCGACGACCAAGCCCGTGTAGAGGACGGAGG11.2048192771084338No Hit
GGATCATATGGATGCTAACGCCATTGGATCAAGGAGGAATAGGCAGGCCA11.2048192771084338No Hit
CGTGCGCGGCGTCCCGCCGGTGCACAACACCGAGGGCGAGACGGCGCTGA11.2048192771084338No Hit
AGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAG11.2048192771084338No Hit
GAGCCCGTCAGCGATGGCGGGCCCAGATGGATGGCCGTCACCTCGCGCTG11.2048192771084338No Hit
CGCCAATGCGACGCCGGTCGGGAACACGACGGCGCTCCGGACCTGTCTCT11.2048192771084338No Hit
TTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAATT11.2048192771084338No Hit
CAGGAGGACCGTCAGCAGAGCGCCTCGCGTCGGCTGCACCTGCATCTGTC11.2048192771084338No Hit
CTTATACACCTCTGACGCTGCCGACGAACATCTAGGTGTAGATTCCGGGG11.2048192771084338No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT11.2048192771084338No Hit
GTTTAGACGGGCTCACATCACCCCATAAACAAATAGGTTTGGTCCTAGCC11.2048192771084338No Hit
ATACACATCTGACCCTGCCGACTATAATTCGTGTGTATATCTCGGTTTTC11.2048192771084338No Hit
GCAGCTCCTACTATACAAGGCGTCCCCTCCAGACACGACACCGGGCCATT11.2048192771084338No Hit
ACCTCGGCTACCCGTNCCTTGTACTATCGCTATGCGGCATAAGTATGACA11.2048192771084338No Hit
TTACTAACAACATTTCCCCCGCATCCCCCTTCCAAACAACAATCCCCCTC11.2048192771084338No Hit
GGGCCGGTCGAAGTCAACGGGGAGGCCTAGGCTAACGCCGACGTATGTGT11.2048192771084338No Hit
CGCTCGATCAGCTCGGGCTCGGCCTGGCGCTCGGCGAGCTTGCGTTCGAG11.2048192771084338No Hit
GTTAGTAGTATGGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAG11.2048192771084338No Hit
CGTATCACTGCAATGATCACCTAGGTGATGTAAGCCTTGTATCTGTCTCT11.2048192771084338No Hit
CGATGTGGCAGTGCACGCCCAGCAGCTCGATCGACTCGGCGGCCAGGGCC11.2048192771084338No Hit
GACTCTCATAGTCGAGCCGCTACGCTTTCATAAGCCCCCAAACCCGCCAC11.2048192771084338No Hit
TCGATGACGTCGTCCGTGAACGCGGCCGGGTGGGGGCTGGTGAAGCGGAC11.2048192771084338No Hit
GGTGTTCCGTCGCGTGGGAATGGTGGTGGCCGAGATCCGCCGGGGGGGGC11.2048192771084338No Hit
CACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAACACAGCCGTATTAC11.2048192771084338No Hit
AGACGGCGGTGAAGAAGTAGCCGAAGGAAGAGGTGCACACCCTGCTGCGG11.2048192771084338No Hit
GCTTTTCGCCGGCGGCCCCTCCGCCCGATTGGCACGGTGGGGACGGTCCG11.2048192771084338No Hit
TAATACACCAGTCTTGTAAACCGAAGATGAAAACCTTTTTCCAAGGACAA11.2048192771084338No Hit
ATACTCACCTGACCTCTTGCCAACCTACTACTACTCCTTCAAGCATCTTG11.2048192771084338No Hit
GCCGGACGCCGCCCGTCGCCTCACCCGGACGATGGTGCGCTGGGGCTGTC11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG50.044.010
GCGGGAC50.044.039
GGTGAGC50.044.028
ACGCCGT50.044.014
GCCTCGA50.044.08
ATCTCGG50.044.023
TCGACGC50.044.011
TCGATCT50.044.020
TCGGTGA50.044.026
CCTCGAC50.044.09
GTCGATC50.044.019
AGGGCCT50.044.05
CCGTCGA50.044.017
CGAGGGC50.044.03
GTGAGCA50.044.029
GAGCAGC50.044.031
AGCAGCT50.044.032
GCAGCTG50.044.033
CTGCGGG50.044.037
GGCCTCG50.044.07
Produced by FastQC (version 0.11.5)