FastQCFastQC Report
Wed 25 May 2016
SRR1780657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTC11.2048192771084338RNA PCR Primer, Index 14 (95% over 22bp)
CTTGAACCCAGGAGACAGAGGTTACAATGAGCTGAGATTGCATCACTGCA11.2048192771084338No Hit
CGTGTGTCTACGTCTATTCCTACTGTAAATATATGGTGTGCTCACACGAT11.2048192771084338No Hit
CTTATACACATCTCAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT11.2048192771084338No Hit
CTCCTGGCCTGTAAGACTTCTGCTAGGAGTCTGCTGATAGCTTTATGGAT11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACAAGACTTTAATGCATCTCGTATGCCGT11.2048192771084338No Hit
GGCCCAGGCCCTGGTCGCGACGGGGGGGGGCGGCGCCGTCGTCACCGAGG11.2048192771084338No Hit
CTTATACACATATCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT11.2048192771084338No Hit
GGCCCACCGAGACCGCGATGTCCCGCAGCAGCGTGTGCACCTCCTCCGGC11.2048192771084338No Hit
GCCCCGGGTCCGGGTGGCGTCCGCGTCCAGCAGCCGGGCTCGCCTCCACC11.2048192771084338No Hit
CGCGAGGTGACGGCCATCCATCTGGGCCCGCCATCGCTGACGGGCTCCTG11.2048192771084338No Hit
GAGTGAGGAGAAGGCTTACGTCTAGTGAGGGAGAGATTTGGTATATGATT11.2048192771084338No Hit
GCGTAGCCGTCGAGGTCCTGGAAGGGGTCCTGGGCCTGAAACGCCCCGAC11.2048192771084338No Hit
CCCCCGCTCCCGCGGCCAGCTCGAACGCAAGCTCGCCGAGCGCCAGGCCG11.2048192771084338No Hit
CATCATGAAGCTGAAGAAAGGCTCAAAAAGGCTATGCTATACACCAACTG11.2048192771084338No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG11.2048192771084338RNA PCR Primer, Index 46 (96% over 28bp)
GCCCATGTCCCACGTGGGGTGGGCGAGGGCCTGGGCCTGTCTCTTATACA11.2048192771084338No Hit
GATCCCGAACGCGTGGGAGCGCGGGAATCCGGAGACGTGGCGCAGGTGGT11.2048192771084338No Hit
CTCGAGATCCCGCCGCACCTGGGCTACGGCGCCCGCGGCGCCGGCTCCGC11.2048192771084338No Hit
ACTTCATGTCGTTGTTGCAAAAGAGCCTGCAAGCCCTGTCTCTTATACAC11.2048192771084338No Hit
GCTGCTGCCGCTACTGCTGCGGCTCCGCCTCCCGCTCGCGGGCGGGGGCG11.2048192771084338No Hit
ATACAAGGCTTACATCACCTAGGTGATCATTGCAGTGATACGCTGTCTCT11.2048192771084338No Hit
GGCGCACCCCGGCAAGCAGCTGCTGTTCATGGGCGGGGAGTACGCGCAGC11.2048192771084338No Hit
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG11.2048192771084338Illumina PCR Primer Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACAGACCGGTTCCCATCTCGTATGCCGTC11.2048192771084338No Hit
GTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTTTGAGTTTGA11.2048192771084338No Hit
GCTAACCCCATACCCCGAACCAACCAAACCCCAAAGACACCCCCCACCTG11.2048192771084338No Hit
CTTGAGGATCGTGCCGTGCGCCTCGCTGATCGCCTTCACCCAGATGTGGG11.2048192771084338No Hit
GAATGGAATGGATTGGAATGGATTGCAATGGAATGAAATGGAATGGAATG11.2048192771084338No Hit
CCGTGGAGCGGGACGCCGTCGTGGCGGACCGCGAGTCCGGGGTGTTCGCG11.2048192771084338No Hit
CGCAACCCCGATCACGCCGAGGACGTCCGCGCCGCCGGCGCCGAACCTGT11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCCT11.2048192771084338No Hit
ATCGAGGGCCTCGAACGCGTCCGCTTCACCAGCCCCCACCCGGCCGCGTT11.2048192771084338No Hit
ATGTAACGAACAATGCTACAGGGATGAATATTATGGAGAAGTAGTCTAGT11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTTGTATATCGG11.2048192771084338No Hit
GCCCTGGCCGCCGAGTCGATCGAGCTGCTGGGCGTGCACTGCCACATCGC11.2048192771084338No Hit
GCCCACACCGGCCCGCCCCGGCGCTCGGCCACGAGGCGTTCGAGCGCGGC11.2048192771084338No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG11.2048192771084338No Hit
ACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCC11.2048192771084338No Hit
AGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAG11.2048192771084338No Hit
ATGTCTAACAACATGGCTTTCTCAACTTTTAAAGGATAACAGCTATCCAT11.2048192771084338No Hit
CCCCGAACGTCTGCCCGAAGGTGCCCATCATCACCACGGTGTCGAGGATC11.2048192771084338No Hit
GCGCTGCGCTTCTACTTCGAGTTCCACCACGACTACGACCTGTCTCTTAT11.2048192771084338No Hit
CCCCAGCGCACCATCGTCCGGGTGAGGCGACGGGCGGCGTCCGGCCTGTC11.2048192771084338No Hit
GAGAGGTGCCAGAGGCTGTGGCTGTCAGTCCTGTATCTGCCCAAGACTCA11.2048192771084338No Hit
GTCCACGCAGCTGTCCGGCCTGCCGTTCTCCGGTCCCATCGGCGGCGTGC11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGT11.2048192771084338No Hit
ATACACATCTCCGAGCCCACGAACACCGGCTAATCTCGTATGCCGTCTTC11.2048192771084338Illumina PCR Primer Index 10 (95% over 22bp)
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC11.2048192771084338No Hit
CCCCTCACCCACTAGGATACCAACAAACCTACCCATCTTTAACAGTACAT11.2048192771084338No Hit
GGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCAT11.2048192771084338No Hit
AATAGGAAGTATGTGCCTGCGTTCAGGCGTTCTGGCTGGTTGCCTCATCG11.2048192771084338No Hit
CAGTACATAGTACATAAAGCCATCTACCGTACATAGCACATTACAGTCAA11.2048192771084338No Hit
GATCTGCTCGCCGCGCACGTCCACCTGGATGACCGTGGCGTCCTCGGGGT11.2048192771084338No Hit
GTCTCGATCAGCGCCGTCTCGCCCTCGGTGTTGTGCACCGGCGGGACGCC11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGACATGATGATATCTCGTATGCCGTCT11.2048192771084338TruSeq Adapter, Index 25 (95% over 21bp)
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG11.2048192771084338No Hit
GGTAGAGGGGGATTGTTGTTTGGAAGGGGGATGCGGGGGAAATGTTGTTA11.2048192771084338No Hit
TCCGCCAGCAGGTCGAAGCAGCGCTCGACGCCCAGGTCCTTGCCGGAGGA11.2048192771084338No Hit
ACTGGAACACCGCATGGAAGCGCCACACCTGAGAAGGGCGTCCGAGCCCA11.2048192771084338No Hit
CGGTGTGGCCTGCCTATTCCTCCTTGATCCAATGGCGTTAGCATCCATAT11.2048192771084338No Hit
GCTCGGACGCGCAGCTCGTGCGCTTCGGCCGGGACCGGGCCCGGATCGCC11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCAGAGACCGGTTCCCATCTCGTATGCCGTC11.2048192771084338No Hit
CTCGAGGGCGTGATGGACGCCCGCGCCGCGTGGCCGGACCACACGTGGGT11.2048192771084338No Hit
CGCTGAACCCGTCGCCGACCGCGCCGGCCCGTCCGCCCGACGCGTCGTCG11.2048192771084338No Hit
GTCTTTATACACATCTCCGACCCACGAGACACCGGCTAATCTCGTATGCC11.2048192771084338No Hit
TAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTC11.2048192771084338No Hit
GCGCAGGGCGCCGCGACCGAAGGGGCGGATCGTCTGGCCCCTGTCTCTTA11.2048192771084338No Hit
GGGCCTTGCGGATGGGGTTGAGAATGACGAATCCGGAGTCGCAGAACACG11.2048192771084338No Hit
CTCCTCGGACGTGGAGACCACGATCGTCCCGCAGCTGCTCACCGAGATCG11.2048192771084338No Hit
CCTCGTGGCCGCGCACGTGCCGCCCGTCCGGCTGATCGACACCATGGCTG11.2048192771084338No Hit
ATGCAGGTGCAGCCGACGCGAGGCGCTCTGCTGACGGTCCTCCTGCTGTC11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT11.2048192771084338No Hit
GTCCGGAGCGCCGTCGTGTTCCCGACCGGCGTCGCATTGGCGCTGTCTCT11.2048192771084338No Hit
GTCGTCGGCCGATCCACACCGGGAGGACGGTGTGATCGAGATCTGCGCCC11.2048192771084338No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA11.2048192771084338No Hit
GATTTGTCCTTGGAAAAAGGTTTTCATCTTCGGTTTACAAGACTGGTGTA11.2048192771084338No Hit
GACATCACGGACTCGCGCTTGGCGGCCTCGTCCAGGGTCTGTCTCTTATA11.2048192771084338No Hit
CATCGAAACCGCAAACATATCATACACATCTTCCGAGCCCACGAGACTTT11.2048192771084338No Hit
CAACACCCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCAAG11.2048192771084338No Hit
CGAAGGCCCACTGAGCCCACACGGTCTGCTCAGGCGGGACGCAGCGCGGT11.2048192771084338No Hit
CCCTTGACCACCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCTAC11.2048192771084338No Hit
AATACATTTTTGCCAATGAGAAATAAGTTTGTTTAATCCTGTAGCATAAA11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGACC50.044.013
TCCTCGG50.044.02
CTCCTCG50.044.01
ACCACGA50.044.017
CCACGAT50.044.018
CCTCGGA50.044.03
CACCGAG50.044.040
GATCGTC50.044.022
CACGATC50.044.019
CGGACGT50.044.06
TCGGACG50.044.05
CCGCAGC50.044.029
TGGAGAC50.044.012
ACCGAGA50.044.041
CGTGGAG50.044.010
GCAGCTG50.044.031
CCCGCAG50.044.028
GCTGCTC50.044.034
GTCCCGC50.044.026
GACGTGG50.044.08