FastQCFastQC Report
Wed 25 May 2016
SRR1780656_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780656_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28962
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC290.10013120640839722No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATGG200.006885464352.49741429
TCTTTCA200.006885464352.49741457
ACGAATT901.6612285E-731.10957767
AATTGAA703.377107E-529.99851870
GAATTGA755.4067677E-527.99861769
CGAATTG905.9100694E-627.2208868
GACGAAT1109.612559E-725.45328966
CGACGAA1451.0574076E-519.30939165
CCGACGA1806.777026E-515.55478964
GCCGACG1951.3411416E-414.35826663
TGCCGAC2254.5079185E-412.4438362
CTGCCGA2407.759404E-411.6660961
GCTGCCG2550.001289401410.97985160
CGCTGCC2750.00241915110.18131559
Produced by FastQC (version 0.11.5)