##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780656_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28962 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.286893170361164 34.0 27.0 34.0 12.0 34.0 2 30.67467716317934 34.0 32.0 34.0 21.0 34.0 3 30.80256888336441 34.0 32.0 34.0 21.0 34.0 4 31.108486982943166 34.0 33.0 34.0 21.0 34.0 5 31.119121607623782 34.0 34.0 34.0 21.0 34.0 6 33.55880118776328 38.0 34.0 38.0 21.0 38.0 7 33.20758234928527 37.0 34.0 38.0 21.0 38.0 8 32.167495338719704 37.0 31.0 38.0 11.0 38.0 9 33.03007388992473 37.0 34.0 38.0 21.0 38.0 10-11 33.22753953456253 37.0 34.0 38.0 21.0 38.0 12-13 33.57060976451903 38.0 34.0 38.0 21.0 38.0 14-15 33.19651612457703 37.0 34.0 38.0 16.0 38.0 16-17 33.01961190525516 37.0 34.0 38.0 11.5 38.0 18-19 33.04455838685173 37.0 33.0 38.0 16.0 38.0 20-21 33.07235342863062 37.0 34.0 38.0 16.0 38.0 22-23 33.049150611145635 37.0 34.0 38.0 11.0 38.0 24-25 33.485239279055314 37.5 34.0 38.0 21.0 38.0 26-27 33.447500172640005 38.0 34.0 38.0 21.0 38.0 28-29 33.56993646847593 38.0 34.0 38.0 21.0 38.0 30-31 33.437228091982604 38.0 34.0 38.0 21.0 38.0 32-33 33.199312892756026 37.0 34.0 38.0 16.0 38.0 34-35 33.11679096747462 37.0 34.0 38.0 16.0 38.0 36-37 33.055952627580965 37.0 34.0 38.0 16.0 38.0 38-39 33.50752710448174 37.5 34.0 38.0 21.0 38.0 40-41 33.11130101512326 37.0 34.0 38.0 11.0 38.0 42-43 33.377425592155234 37.0 34.0 38.0 21.0 38.0 44-45 32.56384227608591 37.0 31.5 38.0 11.0 38.0 46-47 33.28860230647055 37.0 34.0 38.0 21.0 38.0 48-49 32.999015951937025 37.0 33.5 38.0 16.0 38.0 50-51 32.9224155790346 37.0 33.0 38.0 16.0 38.0 52-53 32.985083903045364 37.0 33.5 38.0 11.0 38.0 54-55 32.7048546371107 37.0 31.5 38.0 11.0 38.0 56-57 32.72151439817692 37.0 32.0 38.0 11.0 38.0 58-59 32.59878461432221 37.0 31.5 38.0 11.0 38.0 60-61 32.59711000621504 37.0 31.5 38.0 11.0 38.0 62-63 32.65042124162696 37.0 32.0 38.0 11.0 38.0 64-65 32.63930322491541 37.0 31.5 38.0 11.0 38.0 66-67 32.63700711276846 37.0 31.5 38.0 11.0 38.0 68-69 32.21160486154271 37.0 30.5 38.0 11.0 38.0 70-71 32.09032525378082 37.0 31.0 38.0 11.0 38.0 72-73 32.17688695532077 37.0 31.0 38.0 11.0 38.0 74-75 31.80736827567157 37.0 29.5 38.0 11.0 38.0 76 31.17405565913956 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.2278291225374574 1101 2 -0.124965795573452 1101 3 -0.36633859104600575 1101 4 -3.105327985252888 1101 5 -0.1212257720761798 1101 6 -1.236118373973703 1101 7 -0.6269374829270689 1101 8 -0.7745253244270245 1101 9 0.11405315186392784 1101 10-11 -0.283174791932602 1101 12-13 -0.5856516480463156 1101 14-15 -0.09601977003899975 1101 16-17 -1.4887771765999638 1101 18-19 -0.4973249036185905 1101 20-21 -0.04467195699812265 1101 22-23 0.5913590500346828 1101 24-25 0.21238630587443197 1101 26-27 0.07752222949871168 1101 28-29 0.6481102161994983 1101 30-31 0.09239292865816395 1101 32-33 0.6964765883964574 1101 34-35 0.12816575611417136 1101 36-37 0.7809779205339069 1101 38-39 -0.13598054836667473 1101 40-41 2.2785402930385104 1101 42-43 0.41514380881415747 1101 44-45 1.155252920498711 1101 46-47 0.5823343911811776 1101 48-49 -0.08602772228384481 1101 50-51 -0.39904710272997335 1101 52-53 -1.114048035610601 1101 54-55 0.23723164641731387 1101 56-57 0.8146741227135337 1101 58-59 -0.5814088932722257 1101 60-61 0.12423947978120253 1101 62-63 -0.9082284034874313 1101 64-65 0.005402047468692217 1101 66-67 0.7984425958216619 1101 68-69 1.1301177831454439 1101 70-71 0.7657012298796388 1101 72-73 1.4560298100914792 1101 74-75 -0.7491866795724391 1101 76 -1.2889129784723181 1102 1 -0.6107702990080455 1102 2 -0.44516187400482465 1102 3 -0.3022209439871837 1102 4 0.05467201474711203 1102 5 0.9334801102767614 1102 6 -0.43690268769919527 1102 7 1.3558076151121483 1102 8 1.0858668324357197 1102 9 -0.31359390695960343 1102 10-11 -0.9404296938933854 1102 12-13 -4.691828118634547 1102 14-15 -1.0290589857252783 1102 16-17 -1.1756399216980071 1102 18-19 0.06747901795004196 1102 20-21 0.22895549398226933 1102 22-23 -0.945797812710417 1102 24-25 0.03777846273717955 1102 26-27 -6.162673848932666 1102 28-29 -0.35796821517305233 1102 30-31 -0.05790118898889318 1102 32-33 -7.345876352780014 1102 34-35 -1.4142852242779895 1102 36-37 -0.8699044324072673 1102 38-39 0.5860782751627411 1102 40-41 0.3355010773522409 1102 42-43 -0.6157385441270193 1102 44-45 -5.242786295187564 1102 46-47 -0.022763648034512585 1102 48-49 0.633972277716154 1102 50-51 -5.600615730180959 1102 52-53 -0.7649303885517753 1102 54-55 0.33536890131927066 1102 56-57 -1.3000317596394098 1102 58-59 0.6887871851591498 1102 60-61 1.25316104840865 1102 62-63 -4.365385266232529 1102 64-65 -0.7566567760607228 1102 66-67 0.04216808601773181 1102 68-69 -7.567137118815339 1102 70-71 -0.5593968093360431 1102 72-73 -0.030734895790878625 1102 74-75 -6.4957553070234155 1102 76 -2.278324743178203 1103 1 -0.19824017852611675 1103 2 0.554516840854614 1103 3 -0.8945101006136902 1103 4 -0.06918340693963643 1103 5 0.8452873391924243 1103 6 -0.9519227680204807 1103 7 1.1193417516583324 1103 8 1.2942202460903403 1103 9 0.8247193460524471 1103 10-11 0.4028634787973786 1103 12-13 -1.543394383694789 1103 14-15 -0.22970155600639686 1103 16-17 -0.02013791366587725 1103 18-19 -0.06633624309413477 1103 20-21 -0.1301609718811889 1103 22-23 0.4617523880927905 1103 24-25 1.3398668161508382 1103 26-27 -0.47793489310937076 1103 28-29 0.8024333912526487 1103 30-31 0.4881229073966509 1103 32-33 -4.740093220249896 1103 34-35 -0.32408442106513746 1103 36-37 -0.02580804686510163 1103 38-39 1.1872830944398416 1103 40-41 0.9611235673120788 1103 42-43 0.5481168775597283 1103 44-45 -4.597404769083148 1103 46-47 0.11032872144340189 1103 48-49 0.29349035000531387 1103 50-51 0.7054886874897264 1103 52-53 0.8261539487976179 1103 54-55 0.5838829575441693 1103 56-57 1.0710525777099846 1103 58-59 0.7360964221069324 1103 60-61 1.3222373536295393 1103 62-63 0.9862613201128525 1103 64-65 1.0556323805657897 1103 66-67 0.412368889230585 1103 68-69 -2.50312105455831 1103 70-71 0.05273170472018762 1103 72-73 0.4343253451729794 1103 74-75 -1.17326534718406 1103 76 -0.8725416106480814 1104 1 -1.98899814710931 1104 2 -0.7238116630343612 1104 3 0.24107019525332163 1104 4 0.451634040063567 1104 5 -0.5368996365586796 1104 6 -0.6188436159692401 1104 7 0.7126008640572934 1104 8 1.3155714737859299 1104 9 -0.3105559322760598 1104 10-11 -0.3993326474798806 1104 12-13 -0.08220786546998937 1104 14-15 0.17566675267135423 1104 16-17 -0.6572432972254276 1104 18-19 0.3452849251230319 1104 20-21 1.0624576036869087 1104 22-23 0.8569026092305094 1104 24-25 0.8388755091506823 1104 26-27 0.6495624379871714 1104 28-29 0.03958452744298313 1104 30-31 -0.030559416837000697 1104 32-33 -0.3187877451850767 1104 34-35 0.31347848880218265 1104 36-37 0.3551588587319756 1104 38-39 0.06329346503616051 1104 40-41 0.5027584613184786 1104 42-43 0.4513500634679204 1104 44-45 -2.198060556790942 1104 46-47 0.7718355080836332 1104 48-49 0.6987824042984343 1104 50-51 0.7975277297346537 1104 52-53 0.1879810038532881 1104 54-55 0.750727551108298 1104 56-57 1.1607277340314752 1104 58-59 0.7231331767203315 1104 60-61 0.05527075305000295 1104 62-63 0.5788341430501731 1104 64-65 0.021317907483584975 1104 66-67 0.4296786345409416 1104 68-69 -3.340470452148672 1104 70-71 0.037480827794759364 1104 72-73 0.6249613067407651 1104 74-75 0.21479321618333813 1104 76 -0.39427411026681014 1105 1 -1.0749563455196736 1105 2 0.7549931647548647 1105 3 0.5963837071756082 1105 4 0.9411836426540852 1105 5 0.10278243585815616 1105 6 0.7372058394325904 1105 7 2.321078932941603 1105 8 0.7329986153814616 1105 9 1.3975688837380744 1105 10-11 1.6241439495174674 1105 12-13 -0.5064792814252428 1105 14-15 0.8224138824917802 1105 16-17 1.8330422488446274 1105 18-19 1.3680991729887992 1105 20-21 0.8621337885559157 1105 22-23 1.0761401717857098 1105 24-25 1.2693288503340767 1105 26-27 1.3702718875014455 1105 28-29 -0.12548759501801499 1105 30-31 0.4297732296157548 1105 32-33 0.4420306239641718 1105 34-35 0.9009085741716234 1105 36-37 1.4093978931741304 1105 38-39 1.2756131588836652 1105 40-41 0.5283692944064953 1105 42-43 0.9389126186636787 1105 44-45 1.9312447125643715 1105 46-47 1.3217324759964981 1105 48-49 0.693042045158009 1105 50-51 1.4429501612919111 1105 52-53 0.008557983541251701 1105 54-55 0.7712603741874915 1105 56-57 1.1836891705931478 1105 58-59 1.445608890585497 1105 60-61 0.4465719011218283 1105 62-63 0.8556224857054531 1105 64-65 1.0531106657997427 1105 66-67 0.5501525821417701 1105 68-69 1.4037155943629571 1105 70-71 1.6620760588810128 1105 72-73 0.6808930111858658 1105 74-75 0.8864152356122403 1105 76 0.4026054893799369 1106 1 -2.546986515224262 1106 2 -1.0633600722030216 1106 3 -2.281680403446643 1106 4 -1.0107333906582951 1106 5 -0.782195565398915 1106 6 -2.165371156167666 1106 7 0.8078796871842187 1106 8 0.30496593153482365 1106 9 -1.0914317447974398 1106 10-11 -0.6388981623618477 1106 12-13 -1.3680443348507652 1106 14-15 -2.100590517256805 1106 16-17 -0.7785228045808879 1106 18-19 -1.3580164775454548 1106 20-21 -1.7451886501618752 1106 22-23 -0.5316536685662783 1106 24-25 -0.4885278435691234 1106 26-27 -1.4149261011849177 1106 28-29 -2.5367069539117892 1106 30-31 -1.7206038916915958 1106 32-33 -1.0102006771043364 1106 34-35 -1.1220329720257354 1106 36-37 -1.3201747026775372 1106 38-39 -1.33689469781023 1106 40-41 -1.4219764001252457 1106 42-43 -0.31438719277566207 1106 44-45 0.23514163274036548 1106 46-47 -0.6469978822687423 1106 48-49 -1.0590006952568132 1106 50-51 -0.6967418563070851 1106 52-53 -1.2557411993625855 1106 54-55 -0.8194058734555014 1106 56-57 -0.23381554342319077 1106 58-59 -1.1362578598859017 1106 60-61 -1.09773985249225 1106 62-63 0.20524536439810248 1106 64-65 -0.16530542470936638 1106 66-67 0.22252844637809233 1106 68-69 -0.5837136953919142 1106 70-71 -0.9298472597864951 1106 72-73 0.0071029420469628235 1106 74-75 -2.0232327845008946 1106 76 0.046540121686660996 1107 1 -0.17692414516189103 1107 2 0.9168894080464582 1107 3 1.29316367139743 1107 4 0.8177489378240352 1107 5 0.5986083154049666 1107 6 0.45694346614696 1107 7 2.0691409484454795 1107 8 1.628943755512644 1107 9 -1.0356451890108822 1107 10-11 0.21418569072199745 1107 12-13 1.0597103429039123 1107 14-15 0.05273588606959834 1107 16-17 1.1364113603532786 1107 18-19 1.5028636333346483 1107 20-21 1.3220324170591908 1107 22-23 0.15753552062291476 1107 24-25 0.11906051401923179 1107 26-27 0.2604030741442642 1107 28-29 -0.6889938561986924 1107 30-31 -0.12046529155299623 1107 32-33 1.121815954912293 1107 34-35 1.6769968270040607 1107 36-37 0.5270186445158132 1107 38-39 0.8091551982396581 1107 40-41 1.0170395388906925 1107 42-43 1.1740050456165747 1107 44-45 -0.25688885929012883 1107 46-47 1.138518403247538 1107 48-49 -0.10525849151461131 1107 50-51 0.6729740134087834 1107 52-53 0.5935311499097651 1107 54-55 1.0515227474731148 1107 56-57 1.1845836249759785 1107 58-59 1.2549410313129883 1107 60-61 0.3672636125112163 1107 62-63 -0.6753852662325279 1107 64-65 1.143599634195688 1107 66-67 1.08652706037671 1107 68-69 1.0185039068256856 1107 70-71 0.7898339598947217 1107 72-73 0.5474702324142484 1107 74-75 0.3773216160535071 1107 76 -0.3439657688192277 1108 1 -1.2538472220849677 1108 2 -0.5702900791330308 1108 3 -0.7837594055256432 1108 4 -1.7078920878169903 1108 5 0.20117241796906882 1108 6 -1.1712616620581713 1108 7 1.1332435125480487 1108 8 0.19304631961520613 1108 9 -0.7920554454211413 1108 10-11 -0.6191476426113312 1108 12-13 -1.0108024776089053 1108 14-15 -0.26777693444322637 1108 16-17 -0.9533322293903126 1108 18-19 -0.6381620076909869 1108 20-21 -0.030531685504911366 1108 22-23 -0.714259351171961 1108 24-25 -2.1501702552115383 1108 26-27 -1.1754943617531808 1108 28-29 0.003313836109001045 1108 30-31 0.014150093062390567 1108 32-33 -0.20510712201078718 1108 34-35 -0.9255672755600415 1108 36-37 -0.9537505862534204 1108 38-39 -0.3575114684270062 1108 40-41 -1.2329604611093004 1108 42-43 -0.4413795697680385 1108 44-45 -0.9556068080080777 1108 46-47 -0.16276364803450605 1108 48-49 0.04217740592127939 1108 50-51 0.08323042366519218 1108 52-53 0.24737730375591838 1108 54-55 -0.6151439191935495 1108 56-57 0.5307374711298181 1108 58-59 -1.0142897379177782 1108 60-61 -0.1391466438990392 1108 62-63 0.10025575940849762 1108 64-65 0.9128304034264616 1108 66-67 -1.2275755037258556 1108 68-69 -0.09047045214867211 1108 70-71 0.4757313957921667 1108 72-73 0.22695741190143082 1108 74-75 -0.48806299933110964 1108 76 0.18167525682179786 1109 1 -0.5688655371032816 1109 2 -0.8175428263857789 1109 3 0.8325409607747218 1109 4 0.299433919509017 1109 5 1.3458610626577112 1109 6 0.42214493134842 1109 7 -0.49038286107833073 1109 8 1.3844382610071477 1109 9 1.353548950183253 1109 10-11 2.198617925154231 1109 12-13 0.8660290242225912 1109 14-15 0.5083219666556786 1109 16-17 -0.07421135026942949 1109 18-19 1.2208123512833708 1109 20-21 1.148955493982271 1109 22-23 1.5861069491943454 1109 24-25 1.2165879865467062 1109 26-27 1.1289928177340016 1109 28-29 -0.67296821517305 1109 30-31 -0.25599642708413484 1109 32-33 1.0462665043628476 1109 34-35 1.11976239476963 1109 36-37 1.7417622342594044 1109 38-39 1.403935418019877 1109 40-41 0.8709772678284295 1109 42-43 0.9652138368253631 1109 44-45 0.6524518000505282 1109 46-47 -0.24609698136784175 1109 48-49 0.4496865634304399 1109 50-51 0.7150985555333236 1109 52-53 0.4955458019244148 1109 54-55 0.008940329890698706 1109 56-57 0.7761587165510662 1109 58-59 -0.3769271005551431 1109 60-61 0.2079229531705593 1109 62-63 -0.6634805043277687 1109 64-65 0.5547717953678486 1109 66-67 0.3562157050653525 1109 68-69 1.5583390716608463 1109 70-71 0.8063174763782399 1109 72-73 -0.4616872767432554 1109 74-75 1.8828161215480108 1109 76 -0.6516580765115343 1110 1 0.6948707266329812 1110 2 0.5707355618926115 1110 3 -0.28375940552564316 1110 4 -0.1566100365349392 1110 5 0.32937754617419657 1110 6 0.7005332097367045 1110 7 1.0306794099839465 1110 8 1.4109950375639286 1110 9 0.22076506739936974 1110 10-11 -0.28581430927800255 1110 12-13 1.0789411121346788 1110 14-15 0.16171024504395604 1110 16-17 -0.0815373575954439 1110 18-19 0.9644020948731153 1110 20-21 0.31562216064893533 1110 22-23 0.8434329565203456 1110 24-25 0.471624616583334 1110 26-27 -1.3998533361121517 1110 28-29 0.07382665662181864 1110 30-31 0.14876547767777737 1110 32-33 0.634636467732804 1110 34-35 -0.3486441986369613 1110 36-37 -0.8383659708688072 1110 38-39 0.8027449418294097 1110 40-41 0.26703953889069254 1110 42-43 0.6611845327960637 1110 44-45 2.0828547304534624 1110 46-47 0.7218517365808808 1110 48-49 1.0934594572033376 1110 50-51 0.3332304236651922 1110 52-53 0.7153260217046338 1110 54-55 -0.7177080217576517 1110 56-57 -0.36028816989581713 1110 58-59 -2.3860846097126505 1110 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2116 60-61 -1.68295006270246 2116 62-63 -0.09205193289919578 2116 64-65 -0.48193455383849937 2116 66-67 -1.950331913982268 2116 68-69 -0.4446371188153364 2116 70-71 -2.604396809336045 2116 72-73 -0.17101267356865435 2116 74-75 -0.8513108625789698 2116 76 -0.5961025209559807 2117 1 -0.39926344969297745 2117 2 0.10972397074403162 2117 3 1.1092859053278836 2117 4 0.8946720147471119 2117 5 -0.39419112259994904 2117 6 -0.47544150048458533 2117 7 -0.8509503757554313 2117 8 -0.7302062269250094 2117 9 0.6847622574239622 2117 10-11 0.3900725892116341 2117 12-13 1.574199278625727 2117 14-15 0.09304147089573434 2117 16-17 0.4102961513613579 2117 18-19 0.7048306161235516 2117 20-21 0.17178654421058326 2117 22-23 1.9486314110338725 2117 24-25 1.5049930746093239 2117 26-27 1.113581858829889 2117 28-29 0.5467806432744311 2117 30-31 0.9547015507371341 2117 32-33 1.7935757020145076 2117 34-35 0.8426097528909651 2117 36-37 -0.3375756652839783 2117 38-39 1.290187864203837 2117 40-41 0.24499879424721627 2117 42-43 -0.333546763305101 2117 44-45 1.8083552573238535 2117 46-47 -0.4184714105915859 2117 48-49 0.3797257023736904 2117 50-51 0.9031285620564873 2117 52-53 1.1030148169276757 2117 54-55 -1.1565032447994525 2117 56-57 -0.8986618966257112 2117 58-59 0.5503396965746745 2117 60-61 0.7746222356232622 2117 62-63 -1.3386272753649493 2117 64-65 -0.3248759541429109 2117 66-67 -0.9414848820187984 2117 68-69 0.7348720136047504 2117 70-71 2.0731146061890726 2117 72-73 1.3062514055789833 2117 74-75 0.3252492591866343 2117 76 0.6816752568217979 2118 1 -0.6922160821405754 2118 2 0.7231915396497932 2118 3 0.39464652589233395 2118 4 0.13563587016879808 2118 5 0.41155239943338984 2118 6 1.4456675934253056 2118 7 1.9145224745498979 2118 8 -4.5371050551144805 2118 9 -1.7535939069596012 2118 10-11 -0.8501485693953938 2118 12-13 1.0108224837750939 2118 14-15 -1.0369304716690522 2118 16-17 0.3232355803100333 2118 18-19 -0.1807940744194383 2118 20-21 -2.901245309230582 2118 22-23 -0.1587295396180508 2118 24-25 -0.02760306336724483 2118 26-27 1.0521855888183396 2118 28-29 -0.9987714280244546 2118 30-31 1.7110016773358439E-4 2118 32-33 0.5671356954127589 2118 34-35 1.0735059403806417 2118 36-37 -0.5077357577084669 2118 38-39 -0.35488558025894434 2118 40-41 -1.7979125772662385 2118 42-43 -0.2229674597896647 2118 44-45 -0.6215011546253137 2118 46-47 -0.5523218809662396 2118 48-49 -1.2306060355368587 2118 50-51 -0.9933064932331703 2118 52-53 -2.3786653283108095 2118 54-55 0.13207572862850725 2118 56-57 1.0168357102401089 2118 58-59 -0.2639035778930676 2118 60-61 -2.0090879475752814 2118 62-63 0.6850565008357421 2118 64-65 -0.4142471375065071 2118 66-67 -1.8105226770344736 2118 68-69 1.2197705117067486 2118 70-71 -2.284617692870178 2118 72-73 -1.095795136754731 2118 74-75 -1.6612171544129737 2118 76 -1.5231440202866366 2119 1 -0.4253648936026373 2119 2 0.3690723602294099 2119 3 -0.3897885115547517 2119 4 -0.2945171744420776 2119 5 -1.1470604302637781 2119 6 -1.0978035886000939 2119 7 -4.124552745248213 2119 8 -1.4247637981949097 2119 9 -3.0508912042568994 2119 10-11 -0.99700627046996 2119 12-13 -1.4085848753913055 2119 14-15 -1.884374301040591 2119 16-17 -1.900144426202509 2119 18-19 -2.3715299910589707 2119 20-21 -2.9478913528645805 2119 22-23 -1.3154374523500572 2119 24-25 -1.1979873030285901 2119 26-27 -1.6581693444281598 2119 28-29 -0.4961664133712489 2119 30-31 -0.8354687565564589 2119 32-33 -1.8277682446719048 2119 34-35 -1.047708647701409 2119 36-37 -1.8539584864613232 2119 38-39 -1.8301379410534793 2119 40-41 -1.9895439676928106 2119 42-43 -1.1792520576405323 2119 44-45 -3.0351286375299082 2119 46-47 -1.9578086930795564 2119 48-49 -0.7684601547162799 2119 50-51 -0.42647158603681845 2119 52-53 -2.3706060642274522 2119 54-55 -2.3869734410230734 2119 56-57 -1.0513831109907557 2119 58-59 0.4245529509249124 2119 60-61 -0.8544966092490043 2119 62-63 -0.6866465274937923 2119 64-65 -0.7936838030877489 2119 66-67 -0.16936344551379534 2119 68-69 -0.6445245062027283 2119 70-71 -1.8555229354621687 2119 72-73 -2.573978139034118 2119 74-75 -0.9962057574738665 2119 76 -0.18318960804306528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 9.0 13 65.0 14 154.0 15 295.0 16 352.0 17 485.0 18 492.0 19 442.0 20 439.0 21 367.0 22 285.0 23 312.0 24 316.0 25 327.0 26 375.0 27 429.0 28 502.0 29 605.0 30 774.0 31 981.0 32 1214.0 33 1629.0 34 2165.0 35 3317.0 36 5706.0 37 6924.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.902247269185274 23.03624852153343 13.236624225979266 28.824879983302026 2 16.279953041916993 23.016366273047442 36.406325530004835 24.29735515503073 3 15.42365858711415 28.52703542573027 29.921966715005873 26.12733927214971 4 11.839651957737725 20.61321731924591 37.797804019059456 29.749326703956907 5 11.55306953939645 37.59408880602169 35.43608866790968 15.416752986672192 6 24.083281541330017 38.38478005662592 20.74442372764312 16.78751467440094 7 22.829915061114566 33.675160555210276 23.2718734893999 20.223050894275257 8 21.13113735239279 35.439541468130656 21.10351495062496 22.3258062288516 9 21.66632138664457 16.314481044126786 22.242939023548097 39.77625854568055 10-11 23.297769491057245 25.877011256128718 27.750155376009943 23.075063876804087 12-13 21.623161383882326 23.45832470133278 27.49464815965748 27.42386575512741 14-15 19.68786686002348 25.80277605137767 24.33878875768248 30.170568330916375 16-17 21.308956563773222 28.280160209930255 26.113528071265797 24.29735515503073 18-19 21.241626959464124 26.88522892065465 26.5779297009875 25.295214418893725 20-21 20.66673572267109 27.311649747945584 25.5645328361301 26.457081693253226 22-23 21.8113389959257 26.84724811822388 27.02852012982529 24.312892756025136 24-25 21.217457357917272 28.592638629928874 25.072508804640563 25.117395207513294 26-27 20.288654098473863 28.715213037773633 26.617636903528762 24.37849596022374 28-29 20.55279331537877 27.65520336993302 27.921068986948416 23.870934327739796 30-31 21.193287756370417 27.643118569159586 27.009529728609905 24.154063945860095 32-33 20.040052482563357 28.668600234790414 26.79890891513017 24.492438367516055 34-35 21.7612733927215 27.891720185070092 26.6780609073959 23.668945514812513 36-37 21.764726192942476 27.176990539327395 26.512326496788894 24.54595677094123 38-39 21.214004557696292 27.208065741316208 27.910710586285475 23.667219114702025 40-41 21.39872936951868 26.58483530142946 27.10275533457634 24.91367999447552 42-43 20.63220772046129 26.472619294247636 28.171397002969407 24.723775982321662 44-45 19.663697258476624 25.937435259995855 28.773910641530282 25.62495683999724 46-47 20.535529314273877 26.14287687314412 28.89648504937504 24.42510876320696 48-49 19.760375664664043 27.239140943305017 28.666873834679922 24.333609557351014 50-51 19.359850839030454 26.726400110489607 27.94523858849527 25.968510461984668 52-53 19.135418824666807 27.297838547061666 27.786409778330224 25.780332849941303 54-55 18.71762999792832 27.38070575236517 27.876182584075686 26.025481665630828 56-57 18.795318002900352 27.64484496927008 27.522270561425316 26.037566466404254 58-59 19.739658863338168 26.904219321870038 27.192528140321805 26.163593674469993 60-61 19.523858849526967 26.151508873696567 27.484289758994546 26.840342517781924 62-63 19.344313238036047 26.082452869276985 28.38201781644914 26.19121607623783 64-65 19.575650852841655 26.93702092396934 26.672881707064427 26.81444651612458 66-67 20.02451488156895 27.37207375181272 26.22401767833713 26.379393688281194 68-69 19.877770872177337 27.176990539327395 26.123886471928735 26.821352116566537 70-71 20.07458048477315 27.392790553138596 25.716456045853185 26.816172916235065 72-73 20.399143705545196 27.176990539327395 26.196395276569294 26.22747047855811 74-75 20.021062081347974 27.491195359436503 25.977142462537117 26.510600096678406 76 20.261031696706027 26.93529452385885 25.985774463089566 26.817899316345557 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 2.5 16 3.0 17 2.0 18 4.0 19 5.0 20 5.5 21 7.0 22 11.0 23 19.0 24 40.5 25 58.0 26 70.0 27 88.5 28 110.0 29 125.0 30 165.5 31 221.0 32 319.5 33 403.0 34 507.0 35 670.0 36 806.5 37 884.0 38 1002.0 39 1218.0 40 1572.0 41 1847.0 42 1866.0 43 1909.0 44 2033.0 45 2196.5 46 2279.0 47 2270.5 48 2143.5 49 1932.0 50 1839.0 51 1659.0 52 1370.0 53 1123.0 54 985.0 55 950.0 56 763.5 57 548.0 58 484.0 59 424.5 60 299.5 61 196.5 62 159.0 63 127.5 64 87.0 65 70.5 66 56.0 67 49.0 68 38.0 69 20.0 70 12.0 71 11.0 72 9.0 73 6.5 74 3.0 75 0.0 76 1.5 77 3.5 78 3.0 79 2.0 80 1.5 81 0.5 82 0.5 83 1.0 84 1.0 85 2.0 86 2.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.5 98 1.0 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7458048477315102 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 28962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.95062495684 #Duplication Level Percentage of deduplicated Percentage of total 1 76.5190579979799 49.69960638077481 2 11.349742172133325 14.743456943581245 3 5.374514911487959 10.472343070229956 4 2.6899154749880387 6.988467647261929 5 1.5097549306256979 4.902976313790484 6 0.8505661580989846 3.3146882121400454 7 0.5688161182286959 2.5861473655134315 8 0.4252830790494923 2.209792141426697 9 0.23922173196533944 1.3983840894965818 >10 0.4731274254425602 3.6841378357848216 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC 29 0.10013120640839722 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006905600441958429 0.0 2 0.0 0.0 0.0 0.006905600441958429 0.0 3 0.0 0.0 0.0 0.010358400662937643 0.0 4 0.0 0.0 0.0 0.017264001104896072 0.0 5 0.0 0.0 0.0 0.017264001104896072 0.0 6 0.0 0.0 0.0 0.017264001104896072 0.0 7 0.0 0.0 0.0 0.027622401767833715 0.0 8 0.0 0.0 0.0 0.027622401767833715 0.0 9 0.0 0.0 0.0 0.06905600441958429 0.0 10 0.0 0.0 0.0 0.08286720530350114 0.0 11 0.0 0.0 0.0 0.08977280574545957 0.0 12 0.0 0.0 0.0 0.10703680685035565 0.0 13 0.0 0.0 0.0 0.10703680685035565 0.0 14 0.0 0.0 0.0 0.12430080795525171 0.0 15 0.0 0.0 0.0 0.13811200883916858 0.0 16 0.0 0.0 0.0 0.13811200883916858 0.0 17 0.0 0.0 0.0 0.13811200883916858 0.0 18 0.0 0.0 0.0 0.13811200883916858 0.0 19 0.0 0.0 0.0 0.15882881016504385 0.0 20 0.0 0.0 0.0 0.16573441060700228 0.0 21 0.0 0.0 0.0 0.1726400110489607 0.0 22 0.0 0.0 0.0 0.17954561149091913 0.0 23 0.0 0.0 0.0 0.17954561149091913 0.0 24 0.0 0.0 0.0 0.17954561149091913 0.0 25 0.0 0.0 0.0 0.18990401215385677 0.0 26 0.0 0.0 0.0 0.20371521303777362 0.0 27 0.0 0.0 0.0 0.2175264139216905 0.0 28 0.0 0.0 0.0 0.2451488156895242 0.0 29 0.0 0.0 0.0 0.2589600165734411 0.0 30 0.0 0.0 0.0 0.27967681789931637 0.0 31 0.0 0.0 0.0 0.3211104205510669 0.0 32 0.0 0.0 0.0 0.3418272218769422 0.0 33 0.0 0.0 0.0 0.37635522408673433 0.0 34 0.0 0.0 0.0 0.41088322629652646 0.0 35 0.0 0.0 0.0 0.5282784338098198 0.0 36 0.0 0.0 0.0 0.6732960430909467 0.0 37 0.0 0.0 0.0 0.9598784614322216 0.0 38 0.0 0.0 0.0 1.3776672881707064 0.0 39 0.0 0.0 0.0 1.8196257164560459 0.0 40 0.0 0.0 0.0 2.3962433533595746 0.0 41 0.0 0.0 0.0 2.997030591809958 0.0 42 0.0 0.0 0.0 3.6185346315862166 0.0 43 0.0 0.0 0.0 4.271113873351288 0.0 44 0.0 0.0 0.0 5.054899523513569 0.0 45 0.0 0.0 0.0 5.645328361301015 0.0 46 0.0 0.0 0.0 6.3186244043919615 0.0 47 0.0 0.0 0.0 6.888336440853532 0.0 48 0.0 0.0 0.0 7.4235204751053105 0.0 49 0.0 0.0 0.0 8.013949312892755 0.0 50 0.0 0.0 0.0 8.61818935156412 0.0 51 0.0 0.0 0.0 9.198259788688626 0.0 52 0.0 0.0 0.0 9.805952627580968 0.0 53 0.0 0.0 0.0 10.41364546647331 0.0 54 0.0 0.0 0.0 10.924659899178234 0.0 55 0.0 0.0 0.0 11.490919135418824 0.0 56 0.0 0.0 0.0 12.146951177404876 0.0 57 0.0 0.0 0.0 12.682135211656654 0.0 58 0.0 0.0 0.0 13.189696844140599 0.0 59 0.0 0.0 0.0 13.728333678613355 0.0 60 0.0 0.0 0.0 14.3187625164008 0.0 61 0.0 0.0 0.0 14.71928734203439 0.0 62 0.0 0.0 0.0 15.181962571645604 0.0 63 0.0 0.0 0.0 15.658449002140737 0.0 64 0.0 0.0 0.0 16.279953041916993 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATGG 20 0.0068854643 52.497414 29 TCTTTCA 20 0.0068854643 52.497414 57 ACGAATT 90 1.6612285E-7 31.109577 67 AATTGAA 70 3.377107E-5 29.998518 70 GAATTGA 75 5.4067677E-5 27.998617 69 CGAATTG 90 5.9100694E-6 27.22088 68 GACGAAT 110 9.612559E-7 25.453289 66 CGACGAA 145 1.0574076E-5 19.309391 65 CCGACGA 180 6.777026E-5 15.554789 64 GCCGACG 195 1.3411416E-4 14.358266 63 TGCCGAC 225 4.5079185E-4 12.44383 62 CTGCCGA 240 7.759404E-4 11.66609 61 GCTGCCG 255 0.0012894014 10.979851 60 CGCTGCC 275 0.002419151 10.181315 59 >>END_MODULE