FastQCFastQC Report
Wed 25 May 2016
SRR1780656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28962
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT8823.045369794903667No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3811.3155168841930807RNA PCR Primer, Index 15 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1280.44195842828533943No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC880.30384641944617086No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG820.2831296181202955No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT410.14156480906014776No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC330.11394240729231406No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT320.11048960707133486No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT310.10703680685035565No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT290.10013120640839722No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTT150.00220300270.05
TGATAAT150.00220300270.030
TTGATAA150.00220300270.029
TTTTGCC200.006884121352.531
ACCGGCT200.006884121352.524
AACCGGC200.006884121352.523
GCCCAAA200.006884121352.535
AATTCAC200.006884121352.558
TTTTTGC200.006884121352.530
TATTGAG200.006884121352.59
TAACCGG200.006884121352.522
GGCTTTT200.006884121352.527
CTAACCG200.006884121352.521
GGCCATT200.006884121352.544
ATTGAGG200.006884121352.510
AATATCG200.006884121352.538
CAAATGG200.006884121352.538
ATCGAAG200.006884121352.551
ATTTAAC200.006884121352.552
CATCTTC305.784432E-446.66666414
Produced by FastQC (version 0.11.5)