##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780656_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28962 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.06425661211242 34.0 34.0 34.0 32.0 34.0 2 33.37583730405359 34.0 34.0 34.0 33.0 34.0 3 33.538809474483806 34.0 34.0 34.0 34.0 34.0 4 33.55548649955114 34.0 34.0 34.0 34.0 34.0 5 33.5352530902562 34.0 34.0 34.0 34.0 34.0 6 37.32014363648919 38.0 38.0 38.0 37.0 38.0 7 36.93232511566881 38.0 37.0 38.0 35.0 38.0 8 36.953076444996896 38.0 37.0 38.0 35.0 38.0 9 36.74000414336027 38.0 37.0 38.0 34.0 38.0 10-11 36.45198881292728 38.0 37.0 38.0 34.0 38.0 12-13 36.33554312547476 38.0 37.0 38.0 34.0 38.0 14-15 36.56869346039638 38.0 37.0 38.0 34.0 38.0 16-17 36.64524204129549 38.0 37.0 38.0 34.0 38.0 18-19 36.663800842483255 38.0 37.0 38.0 34.0 38.0 20-21 36.40827636212968 38.0 37.0 38.0 34.0 38.0 22-23 36.547907603066086 38.0 37.0 38.0 34.0 38.0 24-25 35.90964021821698 38.0 36.5 38.0 32.5 38.0 26-27 36.03777363441751 38.0 37.0 38.0 33.0 38.0 28-29 35.92308887507769 38.0 37.0 38.0 33.0 38.0 30-31 35.67780194737932 38.0 36.5 38.0 31.5 38.0 32-33 36.161642842345145 38.0 37.0 38.0 34.0 38.0 34-35 36.1452765692977 38.0 37.0 38.0 33.5 38.0 36-37 36.257889648504936 38.0 37.0 38.0 34.0 38.0 38-39 35.755731648366826 38.0 36.5 38.0 32.5 38.0 40-41 35.42920033146882 38.0 36.0 38.0 29.0 38.0 42-43 35.93764242800911 38.0 36.5 38.0 32.5 38.0 44-45 36.09572888612665 38.0 37.0 38.0 33.5 38.0 46-47 36.38850908086458 38.0 37.0 38.0 34.0 38.0 48-49 36.18750431600028 38.0 37.0 38.0 34.0 38.0 50-51 36.30481320350805 38.0 37.0 38.0 34.0 38.0 52-53 36.24159243146191 38.0 37.0 38.0 34.0 38.0 54-55 36.38702437676956 38.0 37.0 38.0 34.0 38.0 56-57 36.437141771977075 38.0 37.0 38.0 34.0 38.0 58-59 36.4760030384642 38.0 37.0 38.0 34.0 38.0 60-61 36.16041709826669 38.0 37.0 38.0 33.0 38.0 62-63 36.42547130723017 38.0 37.0 38.0 34.0 38.0 64-65 36.478506318624405 38.0 37.0 38.0 34.0 38.0 66-67 35.79498998687936 38.0 36.5 38.0 31.5 38.0 68-69 35.08488709343277 38.0 36.0 38.0 29.0 38.0 70-71 34.919601546854494 38.0 36.0 38.0 26.0 38.0 72-73 34.38061943235964 38.0 35.0 38.0 23.5 38.0 74-75 34.89013189696844 38.0 36.0 38.0 25.0 38.0 76 34.38781852082039 37.0 35.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12954930005624732 1101 2 -0.1825111696832451 1101 3 0.01536646480619197 1101 4 0.2919839522969312 1101 5 0.14141910976522354 1101 6 -0.9110180396328715 1101 7 -0.05079994742214211 1101 8 0.28327905379799034 1101 9 -0.07478774079236672 1101 10-11 0.37108564605272676 1101 12-13 0.9093541714599738 1101 14-15 0.7572338130339133 1101 16-17 -0.3283921472004465 1101 18-19 -0.7250287651990348 1101 20-21 -0.02232861367014749 1101 22-23 0.8079292324563596 1101 24-25 -0.3218476506474488 1101 26-27 0.07330351493263976 1101 28-29 1.3125432821230874 1101 30-31 0.779280788807398 1101 32-33 0.8697464439597837 1101 34-35 0.5572954804846688 1101 36-37 0.7350669508152876 1101 38-39 0.3108853029912595 1101 40-41 1.051769014105048 1101 42-43 0.8627735127803007 1101 44-45 0.09131516046766563 1101 46-47 0.445857617429283 1101 48-49 -1.0003746314545623 1101 50-51 -0.5125757574082499 1101 52-53 -0.6960250377132411 1101 54-55 -0.18040415458454362 1101 56-57 -0.0017162040929861178 1101 58-59 -0.6172411199242802 1101 60-61 -1.5431499043080308 1101 62-63 -0.24521189980995928 1101 64-65 -0.11381766140367944 1101 66-67 -1.1913183965018277 1101 68-69 0.6018782894176411 1101 70-71 0.7370650105004728 1101 72-73 -0.5491029850100091 1101 74-75 0.25241897562236915 1101 76 -0.08133479889709605 1102 1 -0.44731344504178594 1102 2 0.020822163650088044 1102 3 -0.01149628029184413 1102 4 0.07492512876751789 1102 5 0.127497541137771 1102 6 0.1278054897788934 1102 7 -1.0333489670299798 1102 8 0.11190650477838204 1102 9 0.2073691219527376 1102 10-11 0.026869959778217378 1102 12-13 -0.3064301422655191 1102 14-15 -0.14541324578961223 1102 16-17 0.22229412730936104 1102 18-19 -0.6379699416696241 1102 20-21 0.24169099417299122 1102 22-23 0.4069488402994992 1102 24-25 -4.517435885941566 1102 26-27 -1.8508141321261817 1102 28-29 -0.5972606394455369 1102 30-31 1.0627121613564228 1102 32-33 0.30974644395978146 1102 34-35 0.7647464608768288 1102 36-37 -2.2215016766356968 1102 38-39 -0.23970293230285478 1102 40-41 1.0347101905756375 1102 42-43 -0.08173629114126157 1102 44-45 -0.33692013364998985 1102 46-47 0.7064458527233981 1102 48-49 -2.9499824745918133 1102 50-51 -1.1561051691729602 1102 52-53 -0.7242603318308909 1102 54-55 0.36224290423898253 1102 56-57 0.5957347762991745 1102 58-59 -1.1934175905125173 1102 60-61 -4.7444244141119505 1102 62-63 -1.9609962135354522 1102 64-65 -0.06283726924681332 1102 66-67 0.9997600348707181 1102 68-69 -1.489494259601969 1102 70-71 -1.0594055777348288 1102 72-73 -5.841161808539422 1102 74-75 -1.776012396926653 1102 76 -0.09035440674023221 1103 1 0.16746567142407542 1103 2 0.06146473393120999 1103 3 -0.9075605373199593 1103 4 -0.12314716038910944 1103 5 0.1557706335072524 1103 6 -0.24376077528134488 1103 7 0.42705263939571836 1103 8 0.16315148469806218 1103 9 -0.6093377053565021 1103 10-11 -0.39955574303302654 1103 12-13 -0.35614901776752816 1103 14-15 -0.5215578241028709 1103 16-17 -0.10115968795168584 1103 18-19 0.4882951185713438 1103 20-21 -0.7228069977948763 1103 22-23 0.13907735435572732 1103 24-25 -2.5198455244957856 1103 26-27 -0.6214165417647379 1103 28-29 -1.3639273061122026 1103 30-31 0.2377322416777048 1103 32-33 0.6829994560079768 1103 34-35 -0.4076230170348225 1103 36-37 -2.4088912348686335 1103 38-39 -0.9850041371221323 1103 40-41 -0.5380608937617168 1103 42-43 -0.9790053674464829 1103 44-45 0.8344051675548343 1103 46-47 0.3893374189884611 1103 48-49 -3.079219422382984 1103 50-51 -0.926466614956091 1103 52-53 -0.18185069327667946 1103 54-55 -0.4016125174477594 1103 56-57 -0.023863617275125648 1103 58-59 -1.6502850603920294 1103 60-61 -4.048199514513556 1103 62-63 -1.3420403902422748 1103 64-65 -0.6226766266765367 1103 66-67 -0.6122078366152195 1103 68-69 0.22873867212494048 1103 70-71 0.25336550660252755 1103 72-73 -4.772969037455084 1103 74-75 -2.590068621826255 1103 76 -2.4998323183868187 1104 1 -0.2952459344932663 1104 2 0.21305845056991757 1104 3 0.30259654671237257 1104 4 -0.21266980794134582 1104 5 0.0031093554837653414 1104 6 0.12881814800674363 1104 7 0.5629379528012421 1104 8 0.15781367777416477 1104 9 0.8289724974801587 1104 10-11 -0.19397392207832098 1104 12-13 0.43643905604671573 1104 14-15 0.5842070073749426 1104 16-17 0.6125050977734929 1104 18-19 0.8713971469379729 1104 20-21 0.4312268591518915 1104 22-23 0.5162315407214351 1104 24-25 0.34838689886856145 1104 26-27 0.8772871336965977 1104 28-29 1.3132878837612125 1104 30-31 0.39832397570241085 1104 32-33 0.23683505155472062 1104 34-35 0.060273887037169516 1104 36-37 0.9680341883432035 1104 38-39 1.7453603588363862 1104 40-41 1.9331912032338607 1104 42-43 0.41843248523004206 1104 44-45 1.3195355625525451 1104 46-47 0.8120154729765687 1104 48-49 0.016937356631807177 1104 50-51 0.6575657169029867 1104 52-53 0.08915738968809706 1104 54-55 0.005280878922533816 1104 56-57 0.7069584049911555 1104 58-59 0.02860772594318206 1104 60-61 0.5833392989682125 1104 62-63 0.44642994680209824 1104 64-65 0.9152218024831456 1104 66-67 0.029549064406587888 1104 68-69 1.5713496222545729 1104 70-71 1.5289488526449233 1104 72-73 0.5395976851314614 1104 74-75 0.3881226241703928 1104 76 -0.4130548286811617 1105 1 0.7943919813148028 1105 2 0.4395818535725695 1105 3 0.2790463553670719 1105 4 0.09259954737216702 1105 5 -0.05591331157540225 1105 6 0.2524566525695917 1105 7 0.5049456066134255 1105 8 -0.4930547355316932 1105 9 0.6293846258287061 1105 10-11 0.6274125954371286 1105 12-13 -0.8062751035058326 1105 14-15 0.1715634983964307 1105 16-17 0.3417514916504416 1105 18-19 0.7301695932141001 1105 20-21 0.4850243275063235 1105 22-23 0.17865426665608908 1105 24-25 0.6639594628956473 1105 26-27 0.6803486585714893 1105 28-29 0.8628168799343143 1105 30-31 1.3858129365502165 1105 32-33 0.8464906300063006 1105 34-35 -0.2881217561774321 1105 36-37 0.5741572380929867 1105 38-39 0.5321575328134216 1105 40-41 0.6081985626686546 1105 42-43 0.7878761119595126 1105 44-45 1.0263356803035038 1105 46-47 0.9048179457466574 1105 48-49 0.5711027967260165 1105 50-51 0.8441273889665766 1105 52-53 1.0006233890993386 1105 54-55 0.9866615088901511 1105 56-57 0.26914562901234973 1105 58-59 0.8572800839060974 1105 60-61 0.9440251982911434 1105 62-63 -0.21665512826413647 1105 64-65 0.12592242067566417 1105 66-67 1.027046856576149 1105 68-69 0.08717240706469909 1105 70-71 0.6885013990093611 1105 72-73 1.399768424018717 1105 74-75 -0.39771782328324434 1105 76 1.1758471436473599 1106 1 -0.4912774090057539 1106 2 0.4188401816681093 1106 3 0.40129651250094867 1106 4 0.2597899936323813 1106 5 0.32623627987651105 1106 6 -0.09543775346434558 1106 7 -0.9526282463092599 1106 8 0.4104650633369431 1106 9 -0.6308290762454618 1106 10-11 0.5942573471656019 1106 12-13 1.1996959838606074 1106 14-15 -0.025953786330155992 1106 16-17 0.050582415597645536 1106 18-19 0.4287868150871361 1106 20-21 -0.05551621303422394 1106 22-23 0.23757947093012888 1106 24-25 -0.13365210215778234 1106 26-27 1.1778345165224593 1106 28-29 0.973910531725636 1106 30-31 0.4106400892843496 1106 32-33 -0.6370103127969742 1106 34-35 0.20276447889484928 1106 36-37 0.8934532783192566 1106 38-39 -0.1664596890596144 1106 40-41 0.9182237040891508 1106 42-43 0.5297051503001811 1106 44-45 0.15172851499865914 1106 46-47 0.6191485554261007 1106 48-49 0.8831706785613349 1106 50-51 -0.06583489890268623 1106 52-53 0.31709101952046126 1106 54-55 -0.27559493359885323 1106 56-57 -0.3765174759530794 1106 58-59 0.4584743013793755 1106 60-61 0.7289089192213822 1106 62-63 0.2613461288068919 1106 64-65 -0.894819251228796 1106 66-67 -0.7531228480121612 1106 68-69 0.19528051517280431 1106 70-71 -0.19616233449158216 1106 72-73 -1.4630537004313169 1106 74-75 -0.44312951404377543 1106 76 -0.4366607130465425 1107 1 0.7290968113684642 1107 2 0.1828734457013681 1107 3 0.2879908991953357 1107 4 0.292361026203416 1107 5 -0.38686143322119904 1107 6 0.09652343849684542 1107 7 0.4420356483546328 1107 8 0.46729112016299723 1107 9 0.05454860913221893 1107 10-11 0.7709725238807863 1107 12-13 0.34280062696524993 1107 14-15 0.572279061902691 1107 16-17 1.1469095119247399 1107 18-19 0.9476710839714002 1107 20-21 1.2350243275063235 1107 22-23 0.645410378761035 1107 24-25 0.3389743704686978 1107 26-27 0.4586730473609961 1107 28-29 1.1617137195288194 1107 30-31 1.4763019049461619 1107 32-33 1.3120541362674771 1107 34-35 0.941156717287086 1107 36-37 1.3359342208001976 1107 38-39 1.4172201446202237 1107 40-41 1.404710190575635 1107 42-43 1.3382637088587401 1107 44-45 1.4902593535295026 1107 46-47 0.9677279040054501 1107 48-49 0.9992482946389529 1107 50-51 1.0997922667244708 1107 52-53 0.8952268476562892 1107 54-55 0.8058326478287299 1107 56-57 0.8885552891196866 1107 58-59 0.9255567684618455 1107 60-61 0.9660884064008712 1107 62-63 0.4061832736440323 1107 64-65 1.1192140128044628 1107 66-67 0.5143754194861074 1107 68-69 1.59999291988521 1107 70-71 0.7400816017523582 1107 72-73 1.4608894735118625 1107 74-75 0.5073209364066855 1107 76 0.7286199522341263 1108 1 -0.8350057527340979 1108 2 -0.0350752722473473 1108 3 -0.10944499824056209 1108 4 0.2538994877418759 1108 5 -0.45096399732376113 1108 6 -0.10860476663135898 1108 7 -0.18616947985049848 1108 8 -0.5070678541959808 1108 9 -0.24032318573956957 1108 10-11 -0.03671978381152741 1108 12-13 0.8620313961960235 1108 14-15 -0.14566965604602444 1108 16-17 -0.3338597188444865 1108 18-19 0.2233121096124293 1108 20-21 1.0619474044294037 1108 22-23 -0.16228192893127158 1108 24-25 0.14666667816100443 1108 26-27 -1.605429516741573 1108 28-29 -1.7100811522660493 1108 30-31 -0.22241604377177993 1108 32-33 0.41461823883157933 1108 34-35 0.38987466600503495 1108 36-37 1.0218316566976355 1108 38-39 1.1095278369279171 1108 40-41 0.5713768572423064 1108 42-43 -0.2643003937053692 1108 44-45 -0.49692013364998644 1108 46-47 0.41003559631314346 1108 48-49 0.7107867561774199 1108 50-51 0.5164589333911422 1108 52-53 -0.6175936651642218 1108 54-55 -0.3223724803764014 1108 56-57 -0.3550344544700579 1108 58-59 -0.27957143666635886 1108 60-61 0.47249866281112674 1108 62-63 0.18182429928506139 1108 64-65 0.5038293974198496 1108 66-67 0.32206772717842114 1108 68-69 0.5743518942441881 1108 70-71 -1.824020962350211 1108 72-73 -1.6865464239240353 1108 74-75 -1.0760123969266573 1108 76 -0.7329185093043336 1109 1 -0.31302773075607604 1109 2 0.3532031160310396 1109 3 0.4061227673272043 1109 4 0.3592108430532335 1109 5 0.15892611256634837 1109 6 0.2906626326360424 1109 7 -0.01858706226807527 1109 8 0.48285888573076363 1109 9 0.567369121952737 1109 10-11 0.7068699597782171 1109 12-13 0.44261747678209673 1109 14-15 0.43720580182943536 1109 16-17 0.8039607939760245 1109 18-19 0.3327443440446629 1109 20-21 0.8897862322682286 1109 22-23 0.2974250307756918 1109 24-25 -0.4650549335606158 1109 26-27 0.3982334869214341 1109 28-29 -0.810355877540772 1109 30-31 0.4291407327849939 1109 32-33 0.9709369201502582 1109 34-35 0.012127413257779551 1109 36-37 1.0754030852690661 1109 38-39 0.5426780200780925 1109 40-41 0.8392340000994452 1109 42-43 1.1775494231444483 1109 44-45 0.5685560568262034 1109 46-47 0.791445852723399 1109 48-49 0.5693032396939017 1109 50-51 -0.19324802631581406 1109 52-53 -0.21283176040232377 1109 54-55 -0.3782332862372044 1109 56-57 -0.01712236655797028 1109 58-59 0.12610621901129804 1109 60-61 0.8241470144594771 1109 62-63 0.8356704531312147 1109 64-65 0.7359722545627108 1109 66-67 -0.8583352032245131 1109 68-69 -0.5437799738876805 1109 70-71 0.12011823178898595 1109 72-73 2.667628891472873E-4 1109 74-75 -0.17125049216474508 1109 76 0.012502736116907442 1110 1 -0.38628780401614904 1110 2 0.1444119072398351 1110 3 0.17260628381072252 1110 4 -0.3743056404632483 1110 5 0.39518984883007846 1110 6 -0.21116886919546118 1110 7 0.13434334066232623 1110 8 0.41600906888094613 1110 9 0.6442921988758101 1110 10-11 -0.6392838863756296 1110 12-13 0.5928006269652499 1110 14-15 0.45048419010782226 1110 16-17 -0.051808436793201906 1110 18-19 0.11433775063807161 1110 20-21 1.2350243275063235 1110 22-23 -1.0340768007261474 1110 24-25 -1.0135897320954115 1110 26-27 1.2279038165917626 1110 28-29 -0.556234998419896 1110 30-31 -0.29933912069486013 1110 32-33 0.28000285421619253 1110 34-35 0.6334644095947795 1110 36-37 0.5859342208001976 1110 38-39 0.4620919394920193 1110 40-41 -1.0888795530141024 1110 42-43 -0.3219927013976758 1110 44-45 0.29154140481155366 1110 46-47 0.5061894424669902 1110 48-49 1.262068807459464 1110 50-51 -0.41943850250629566 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-0.19275355604021627 2116 34-35 -1.7596979835676194 2116 36-37 0.39683165669763554 2116 38-39 -4.583314043413964 2116 40-41 -1.0189009205354793 2116 42-43 -1.6131251800301527 2116 44-45 -0.4413645780944293 2116 46-47 -0.5974430361654868 2116 48-49 -0.14498247459182068 2116 50-51 -0.6763829469507385 2116 52-53 -0.10370477627533603 2116 54-55 -0.5280348735387932 2116 56-57 -0.8742652237008315 2116 58-59 -2.8014731460680693 2116 60-61 -2.055813303000839 2116 62-63 -0.018496213535456718 2116 64-65 -1.5656150470245933 2116 66-67 -0.12718440957371513 2116 68-69 -0.655883148490858 2116 70-71 0.5189277555985043 2116 72-73 0.546893747016135 2116 74-75 0.45870982529557125 2116 76 -0.5886877400735671 2117 1 0.7512253677436007 2117 2 -0.007853635436667616 2117 3 0.2911521215346369 2117 4 0.2031443068497083 2117 5 -0.16821022141930086 2117 6 0.23684658566930494 2117 7 0.7467423571709304 2117 8 0.4021348152806681 2117 9 -0.875553252476486 2117 10-11 -0.2611665699021515 2117 12-13 -1.5268867632700847 2117 14-15 0.028285384347370268 2117 16-17 -1.2628200279417854 2117 18-19 -0.0916685718066077 2117 20-21 -0.518400330027923 2117 22-23 0.35809039281091515 2117 24-25 -1.369490680462114 2117 26-27 -1.6625949540439962 2117 28-29 -1.3707766211806955 2117 30-31 -0.6908038203787399 2117 32-33 -1.5480617752183008 2117 34-35 0.5979884700092484 2117 36-37 -0.3651546446722236 2117 38-39 0.35399569783412943 2117 40-41 -0.0713172066846397 2117 42-43 0.13735046684960395 2117 44-45 -1.2571941062527259 2117 46-47 -2.182869215769756 2117 48-49 -1.0553706024456986 2117 50-51 -0.5004887308167909 2117 52-53 0.5787533667992477 2117 54-55 -0.19803106836375406 2117 56-57 0.16568911419871313 2117 58-59 -1.1046504672248432 2117 60-61 -0.3395842314635473 2117 62-63 -0.21569940988248248 2117 64-65 -0.8728829313472772 2117 66-67 -1.0060847139877254 2117 68-69 -0.3546084148531037 2117 70-71 -0.6654329749950989 2117 72-73 0.46650942433728915 2117 74-75 0.44681865330165493 2117 76 0.9609697941730104 2118 1 -0.4469921599012281 2118 2 0.4831514809191617 2118 3 -0.9798496939464627 2118 4 0.10576850226149759 2118 5 0.3726381033867696 2118 6 0.10563681508010347 2118 7 0.6318719165041529 2118 8 0.6691755810836 2118 9 -0.6936750547540953 2118 10-11 -0.38750755026193673 2118 12-13 -0.5248237165627074 2118 14-15 0.5145867542103844 2118 16-17 -3.191521133734817 2118 18-19 -0.8490542790190148 2118 20-21 -0.5541322989997042 2118 22-23 0.6390773543557273 2118 24-25 0.23316652369698687 2118 26-27 -2.7358743730900414 2118 28-29 -0.6892285109314784 2118 30-31 -0.4731111318162746 2118 32-33 -0.3531451223052784 2118 34-35 0.1044251757362673 2118 36-37 -3.0956382228204333 2118 38-39 -0.06331739013418058 2118 40-41 -0.6223982431593029 2118 42-43 0.7258139096619445 2118 44-45 -3.6414984469030003 2118 46-47 -0.26126499065009057 2118 48-49 0.3966841920748507 2118 50-51 -0.5469485426669323 2118 52-53 -1.2541398499031828 2118 54-55 -0.25101010780920774 2118 56-57 0.3436062622429503 2118 58-59 -0.43341759051251216 2118 60-61 -1.1807296349954868 2118 62-63 -1.968546414338661 2118 64-65 -0.3515922893271366 2118 66-67 -0.6844969932417229 2118 68-69 -2.1748757857063836 2118 70-71 -0.6924176259275967 2118 72-73 0.6788381914605779 2118 74-75 0.4761964384147106 2118 76 0.3917339466734191 2119 1 0.7789928612645198 2119 2 -0.14872738589946266 2119 3 -0.3014061902017531 2119 4 -0.4158856820432959 2119 5 0.08299303663326896 2119 6 -0.25759991562650697 2119 7 -1.0607363544173722 2119 8 0.05911371198558868 2119 9 -0.49569394111032494 2119 10-11 -0.8111480582398016 2119 12-13 0.34834463250925296 2119 14-15 0.35242459204822296 2119 16-17 -0.023741908726677252 2119 18-19 0.3477057340060554 2119 20-21 -0.8798405373585467 2119 22-23 -0.06647458312392729 2119 24-25 0.43391546540978965 2119 26-27 0.02918586787381372 2119 28-29 -0.053116495301395616 2119 30-31 0.37009954874380213 2119 32-33 0.39001671423005746 2119 34-35 0.8108725870029545 2119 36-37 0.6299397648057479 2119 38-39 -1.81510833770826 2119 40-41 -1.3317762959108492 2119 42-43 0.3945700151650371 2119 44-45 0.4355122987824487 2119 46-47 -0.03625684997930989 2119 48-49 -1.2789914836008265 2119 50-51 0.6165975335297489 2119 52-53 0.1819558843853244 2119 54-55 0.055486147482227466 2119 56-57 -0.7954363948719987 2119 58-59 0.04631213921721411 2119 60-61 0.39107108138354363 2119 62-63 -0.2994646820039151 2119 64-65 -0.11103546744502069 2119 66-67 0.5036339087445967 2119 68-69 1.4114967313890219 2119 70-71 0.857891719562474 2119 72-73 -0.050891538269148384 2119 74-75 -1.1120484329626876 2119 76 -0.30152557791140566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 0.0 23 4.0 24 8.0 25 23.0 26 53.0 27 88.0 28 162.0 29 290.0 30 415.0 31 657.0 32 1075.0 33 1731.0 34 2541.0 35 3633.0 36 6479.0 37 11801.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.40846626614184 23.50321110420551 10.344589462053726 30.743733167598926 2 16.355914646778537 17.80263793936883 42.60410192666252 23.23734548719011 3 15.665354602582696 25.288308818451767 33.48180374283544 25.5645328361301 4 11.812029555969891 22.18769422001243 38.49872246391824 27.50155376009944 5 11.494371935639803 37.39382639320489 38.39859125750984 12.713210413645468 6 24.30426075547269 42.53504592224294 18.424141979145087 14.736551343139286 7 23.75871832055797 31.524066017540225 22.94385746840688 21.773358193494925 8 20.595953318141014 38.854360886679096 20.678820523444514 19.870865271735376 9 21.48332297493267 14.691664940266556 22.01160140874249 41.81341067605828 10-11 23.356467094813894 26.263724880878392 28.483875422968026 21.895932601339688 12-13 21.800980595262757 22.127270216145295 28.364753815344244 27.706995373247707 14-15 19.489330847317174 24.097092742213935 24.16787514674401 32.24570126372488 16-17 23.02154547337891 28.3060562115876 24.87569919204475 23.796699122988745 18-19 23.646502313376146 26.79027691457772 24.41647676265451 25.146744009391615 20-21 20.618396519577377 25.997859263862992 24.975830398453144 28.407913818106483 22-23 21.476417374490712 29.13127546440163 25.51964643325737 23.87266072785029 24-25 23.157931082107588 29.160624266279957 24.69097438022236 22.990470271390098 26-27 22.296457426973276 29.861542711138732 25.97023686209516 21.871762999792832 28-29 20.87045093570886 30.04799392307161 26.101443270492368 22.98011187072716 30-31 20.409502106208137 28.83433464539742 28.621987431807195 22.134175816587252 32-33 22.01850700918445 30.001381120088393 26.588288101650438 21.39182376907672 34-35 20.999930943995583 27.06477453214557 31.149437193563976 20.785857330294867 36-37 20.35425730267247 29.371245079759685 27.17353773910641 23.10095987846143 38-39 21.074166148746635 26.13251847248118 28.086803397555414 24.706511981216767 40-41 20.47337891029625 25.38844002486016 29.51626268904081 24.621918375802775 42-43 23.24770388785305 25.0328016020993 29.504177888267385 22.215316621780264 44-45 19.440991644223466 27.175264139216903 29.604309094675784 23.77943512188385 46-47 22.79366065879428 24.749671983979006 28.192113804295282 24.264553552931428 48-49 21.678406187417995 24.223119950279674 28.331952213244943 25.766521649057385 50-51 19.327049236931153 24.894689593260132 30.085974725502385 25.692286444306333 52-53 18.84711000621504 25.47476003038464 32.76362129687176 22.914508666528555 54-55 17.995994751743662 25.60941923900283 30.736827567156965 25.65775844209654 56-57 20.94468614045991 24.94475519646433 28.682411435674332 25.42814722740142 58-59 18.215247565775844 26.420827290932948 31.0130515848353 24.350873558455906 60-61 20.444030108417927 29.153718665837996 27.216697741868657 23.185553483875424 62-63 18.068503556384226 32.09723085422277 26.294800082867205 23.539465506525794 64-65 18.232511566880742 33.019128513224224 24.849803190387405 23.898556729507632 66-67 18.244596367654168 32.92762930736828 24.668531178785997 24.15924314619156 68-69 18.116842759477937 32.14384365720599 24.494164767626543 25.24514881568952 70-71 18.520820385332502 31.93149644361577 24.240383951384572 25.30729921966715 72-73 18.731441198812238 30.57109315654996 24.848076790276917 25.84938885436089 74-75 18.760790000690562 30.88357157654858 24.789379186520268 25.56625923624059 76 19.11470202334093 30.464056349699607 25.16746081071749 25.25378081624197 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 1.0 18 2.5 19 3.0 20 1.0 21 0.0 22 4.5 23 12.5 24 19.5 25 23.0 26 38.0 27 71.5 28 119.5 29 149.0 30 198.0 31 243.0 32 314.5 33 390.0 34 470.0 35 677.0 36 953.5 37 1103.0 38 1401.0 39 1778.0 40 1996.0 41 2118.0 42 2101.0 43 2062.5 44 2063.5 45 2182.5 46 2262.0 47 2163.5 48 1951.0 49 1756.0 50 1675.0 51 1526.5 52 1212.5 53 945.5 54 844.0 55 798.0 56 602.0 57 412.5 58 373.0 59 300.5 60 206.0 61 151.5 62 119.0 63 85.5 64 41.5 65 29.5 66 21.5 67 15.0 68 13.5 69 8.5 70 3.5 71 2.0 72 1.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 28962.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.75436779227954 #Duplication Level Percentage of deduplicated Percentage of total 1 60.15803854959013 28.126510600096676 2 17.672254634074296 16.52510185760652 3 9.27553356472934 13.010151232649678 4 4.8888560667602095 9.14301498515296 5 2.7398271914925045 6.4049444099164425 6 1.543460601137287 4.329811477107935 7 1.0634369692046377 3.480422622747048 8 0.7458828742338084 2.789862578551205 9 0.5243335056495089 2.2063393412057177 >10 1.351451148364227 8.594019750017264 >50 0.014769957905619968 0.5869760375664664 >100 0.014769957905619968 1.75747531247842 >500 0.007384978952809984 3.045369794903667 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 882 3.045369794903667 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 381 1.3155168841930807 RNA PCR Primer, Index 15 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 128 0.44195842828533943 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 88 0.30384641944617086 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 82 0.2831296181202955 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT 41 0.14156480906014776 No Hit CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 33 0.11394240729231406 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT 32 0.11048960707133486 No Hit TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT 31 0.10703680685035565 No Hit GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT 29 0.10013120640839722 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006905600441958429 0.0 2 0.0 0.0 0.0 0.006905600441958429 0.0 3 0.0 0.0 0.0 0.010358400662937643 0.0 4 0.0 0.0 0.0 0.017264001104896072 0.0 5 0.0 0.0 0.0 0.017264001104896072 0.0 6 0.0 0.0 0.0 0.017264001104896072 0.0 7 0.0 0.0 0.0 0.027622401767833715 0.0 8 0.0 0.0 0.0 0.027622401767833715 0.0 9 0.0 0.0 0.0 0.0725088046405635 0.0 10 0.0 0.0 0.0 0.08977280574545957 0.0 11 0.0 0.0 0.0 0.09667840618741799 0.0 12 0.0 0.0 0.0 0.1208480077342725 0.0 13 0.0 0.0 0.0 0.12430080795525171 0.0 14 0.0 0.0 0.0 0.13811200883916858 0.0 15 0.0 0.0 0.0 0.15537600994406464 0.0 16 0.0 0.0 0.0 0.15882881016504385 0.0 17 0.0 0.0 0.0 0.15882881016504385 0.0 18 0.0 0.0 0.0 0.15882881016504385 0.0 19 0.0 0.0 0.0 0.17609281126993992 0.0 20 0.0 0.0 0.0 0.18299841171189835 0.0 21 0.0 0.0 0.0 0.18990401215385677 0.0 22 0.0 0.0 0.0 0.2002624128167944 0.0 23 0.0 0.0 0.0 0.21062081347973205 0.0 24 0.0 0.0 0.0 0.21062081347973205 0.0 25 0.0 0.0 0.0 0.2175264139216905 0.0 26 0.0 0.0 0.0 0.23133761480560736 0.0 27 0.0 0.0 0.0 0.241696015468545 0.0 28 0.0 0.0 0.0 0.27622401767833715 0.0 29 0.0 0.0 0.0 0.2865824183412748 0.0 30 0.0 0.0 0.0 0.3107520198881293 0.0 31 0.0 0.0 0.0 0.35909122298183827 0.0 32 0.0 0.0 0.0 0.38326082452869276 0.0 33 0.0 0.0 0.0 0.4316000276224018 0.0 34 0.0 0.0 0.0 0.4626752296112147 0.0 35 0.0 0.0 0.0 0.5766176369035287 0.0 36 0.0 0.0 0.0 0.7388992472895518 0.0 37 0.0 0.0 0.0 1.032387266072785 0.0 38 0.0 0.0 0.0 1.4812512948000829 0.0 39 0.0 0.0 0.0 1.9473793246322768 0.0 40 0.0 0.0 0.0 2.537808162419722 0.0 41 0.0 0.0 0.0 3.128237000207168 0.0 42 0.0 0.0 0.0 3.773910641530281 0.0 43 0.0 0.0 0.0 4.457565085284165 0.0 44 0.0 0.0 0.0 5.289689938540156 0.0 45 0.0 0.0 0.0 5.93191077964229 0.0 46 0.0 0.0 0.0 6.639734824943028 0.0 47 0.0 0.0 0.0 7.216352461846558 0.0 48 0.0 0.0 0.0 7.765347696982253 0.0 49 0.0 0.0 0.0 8.383398936537532 0.0 50 0.0 0.0 0.0 8.997997375871831 0.0 51 0.0 0.0 0.0 9.626407016090049 0.0 52 0.0 0.0 0.0 10.258269456529245 0.0 53 0.0 0.0 0.0 10.886679096747462 0.0 54 0.0 0.0 0.0 11.435674331883158 0.0 55 0.0 0.0 0.0 12.022650369449623 0.0 56 0.0 0.0 0.0 12.69594641254057 0.0 57 0.0 0.0 0.0 13.26565844900214 0.0 58 0.0 0.0 0.0 13.79393688281196 0.0 59 0.0 0.0 0.0 14.360196119052551 0.0 60 0.0 0.0 0.0 14.960983357502935 0.0 61 0.0 0.0 0.0 15.368413783578482 0.0 62 0.0 0.0 0.0 15.851805814515572 0.0 63 0.0 0.0 0.0 16.366273047441474 0.0 64 0.0 0.0 0.0 17.025757889648506 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACTT 15 0.002203002 70.0 5 TGATAAT 15 0.002203002 70.0 30 TTGATAA 15 0.002203002 70.0 29 TTTTGCC 20 0.0068841213 52.5 31 ACCGGCT 20 0.0068841213 52.5 24 AACCGGC 20 0.0068841213 52.5 23 GCCCAAA 20 0.0068841213 52.5 35 AATTCAC 20 0.0068841213 52.5 58 TTTTTGC 20 0.0068841213 52.5 30 TATTGAG 20 0.0068841213 52.5 9 TAACCGG 20 0.0068841213 52.5 22 GGCTTTT 20 0.0068841213 52.5 27 CTAACCG 20 0.0068841213 52.5 21 GGCCATT 20 0.0068841213 52.5 44 ATTGAGG 20 0.0068841213 52.5 10 AATATCG 20 0.0068841213 52.5 38 CAAATGG 20 0.0068841213 52.5 38 ATCGAAG 20 0.0068841213 52.5 51 ATTTAAC 20 0.0068841213 52.5 52 CATCTTC 30 5.784432E-4 46.666664 14 >>END_MODULE