FastQCFastQC Report
Wed 25 May 2016
SRR1780655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGAAGTACGCAGCGCAGGTCCGCCCCGAGTTCCTGGAGCTGTCTCT11.5384615384615385No Hit
CCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGG11.5384615384615385No Hit
CTTATACACATCTGACGCTGCCGACGGGAATGGTCGTGTGGAGCTCGCGG11.5384615384615385No Hit
GTTCCCCGCCCACATGGCCGCGTCGACGAGCTCACGCCTGTCTCTTATAC11.5384615384615385No Hit
GCATGGGGGGCCGCGCTGGCGACCGCGCCCGAGCGGACCGCCCTGGCCCT11.5384615384615385No Hit
CAGTGTGGTTGACGGCCCCGGGGCACATGCGTGGTGCGGCTTTGAGGCGG11.5384615384615385No Hit
CGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGCTGT11.5384615384615385No Hit
GTTCGTCCTTTAGTGTTGTGTATGGTCTGTCTCTTATACACATCTGACGC11.5384615384615385No Hit
GTCATGGATGCCGCCTCGACCGTCCGACCGAAGCTGCGGTTCGTCATCTG11.5384615384615385No Hit
TGCGCATCGTGGGCACCGGGCTGCGCTCCGTGGAGCAGGTCCTGGGCCTG11.5384615384615385No Hit
GAATTTGAGGCTTCATAGCCGAGCGGAAATCCCCTTACCCTGACCCCGGC11.5384615384615385No Hit
CATTGAGCACACCATATATTTACAGTAGGAATAGCCCTAGACACACGAGC11.5384615384615385No Hit
GCCTAGGGTGTTGTGAGTGTAAATCAGTGCGATGAGTAGGGGAAGGGAGC11.5384615384615385No Hit
GTGGTGTTAGGCGCGGGCGTGGATGGTGACGACGTAGCCGTCGGGGTCTC11.5384615384615385No Hit
GTCTGGGATCTTCCCGATCTTCTTCACATCCAGGTGCACCATGTGCCTGT11.5384615384615385No Hit
CCTCGGAGCTCTGGGGGATCTGAGTGCAGCCGCTGAGGGCGAGCATCAGG11.5384615384615385No Hit
GGGATCTGGGTCGGAGTAGCGTTGCGCGGGGGACGAGTGCGGCGTGGTTT11.5384615384615385No Hit
CTGCAGCAGATCATTTCATATTGCTTCCGTGGAGTGTGGCGAGTCAGCTA11.5384615384615385No Hit
CACTTACCACTTACAAAAAGTTACTTGATGGGAGAGCTATTTCTCCTGTC11.5384615384615385No Hit
GCCGCGGCGTCAGCGGCCAGGTCTCTAAACAGGTGAGGCCGGCTGACCCC11.5384615384615385No Hit
GAACTACTATACTCAATTGATCCAAGAACTTTACCAACAGAACAAGTTAC11.5384615384615385No Hit
TCCTACATCTCCGGCACCATGCCGCGCGGCTTCGTGCGCCCCCTCGTGGA11.5384615384615385No Hit
TCCCAGCACTTTGGAAGGCCAAGGTGGACGGATCACGAGGTCAGGACCTC11.5384615384615385No Hit
CTATTGGTGCGGGGGCTTTGTATGACTATGGGCGTTGATTAGTAGTAGTT11.5384615384615385No Hit
CTTCGGCGCCGCCCCCGTCACCCGGCGCGCCGAGATCACCTACGTGGCTG11.5384615384615385No Hit
GTCCCGCTCGGGGTGGACCTTGTCCCCCACCATCGTGAGCGTGGCCGGGA11.5384615384615385No Hit
GTGTTCACGGAGGCCGAGAGGATGTTCACGTGGTTCTCGGAGAGGACCCG11.5384615384615385No Hit
GTTCAAGGGGGAAATTACATGCTTCGTCTCGAGTTAGAATGAAGGAAAAT11.5384615384615385No Hit
GCCCCGGACCGATCGGCGTCCGACTGCGCCCCACCACCGGCCTGTCTCTT11.5384615384615385No Hit
GGCTATTGAGGAGTATCCTGAGGCATGGGGGTCAGGGGTTGAGGTCTTGG11.5384615384615385No Hit
CTCCCACTCCCAGGGCACCAACGTGGTCGGCTCGATGGTCGTCATGGAGG11.5384615384615385No Hit
ACCCGGTGCCGGGCGACCTCCACCGGCCCCTGCGCACCGGCGTCTGTCTC11.5384615384615385No Hit
GTCTGACGCTGCCGACGAATGACCATGTGTAGATCGCGGGGGTCGCCCCG11.5384615384615385No Hit
GGCGATGACGACACGACGGATGATGCGGGACCTCGAAGAGCGCGGCTTGA11.5384615384615385No Hit
GCCGTAGAAGATGTTGTCCCCCAGGACGAGCGCGACATGATCGTCGCCGA11.5384615384615385No Hit
CGCCTGCGGAAACCCCGGCCGCCGCCCCCCGCCGCAAACCACCCCCCCCT11.5384615384615385No Hit
GGTTGGCGCGTCGGGGCCCAATCTCGGCGCATTCAAAGTACTTCCTCGCA11.5384615384615385No Hit
ATTCTGCTCCTGTCAGAGATAACACCTCCAGCGCTCACCGGCTGTCCAGG11.5384615384615385No Hit
ACCCTGTCCCCGGACGCCTTCCTCGCCGCGGCCCGCGCCGAGCTGGACGA11.5384615384615385No Hit
GCCTAGTATGAGGCGCGTTATGGAGTGGCGGTGAAATCCCATGGCTAAGC11.5384615384615385No Hit
ACCTAACCCTCACAAGACGAAAAAAGAATAATTGTAAATATTAGCAGGAA11.5384615384615385No Hit
GGGGGGAGAGAAAAAGAGGAAGAGGAATTCCGCTCATTTAACTAATACAC11.5384615384615385No Hit
GGGCACCTCCATCGAGGCATCGGTGCTGATCACCGTGAACCCTGTCTCTT11.5384615384615385No Hit
GCATTACCGTCGACGCCTCCCAGCGCGACAGCTTCCACACTGTCTCTTAT11.5384615384615385No Hit
GTGGAGGGACGTGCAGTGGGAAACGGTACTCTTGGATCCATTCCAAGTCT11.5384615384615385No Hit
CCTGGAGGCATGTCGAACCGCTGCTGCACGCCGCGGGGCCTGTCTCTTAT11.5384615384615385No Hit
CCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTAAGGAGAGAATGAA11.5384615384615385No Hit
CGCACGACGTCGGCCGCCCGCGCCGACGCGTCCGGCGCTGTCTCTTATAC11.5384615384615385No Hit
CTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGG11.5384615384615385No Hit
GGGGGTCTTAGGCCTGAAAAGTTTTTGTGCAACTCCACCTCAAAGTACTC11.5384615384615385No Hit
GAGCTCAAGGCCGTCGACAAGATCATCGTCCTGGCGCTGTCTCTTATACA11.5384615384615385No Hit
CTCCCAAAGTGCTGGGATTACAAGCATGACCCACTGCACCCGGGCTGGAG11.5384615384615385No Hit
GTATTGAGTGTCAACTTGATTGGATTGAAGGATGCAAAGTATTGTTCCTG11.5384615384615385No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCCTGT11.5384615384615385No Hit
GGGCGATGAGTGGGGGGAGGAATGGGGTGGGGTGTGTATGTTCAAACGGT11.5384615384615385No Hit
GTAGTAAGGTGGAGTGGGTTTGGGGCTAGGTTTAGCTCCTGTCTCTTATA11.5384615384615385No Hit
GGTCAGGGGTTGAGGTCTTGGTGAGTGTTTTAGTGGGGTTAGCGATGGAG11.5384615384615385No Hit
GTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAATAATGGGGGAA11.5384615384615385No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATC11.5384615384615385No Hit
GGCGTGCCCGCGCATCTCGCTCGAGCCGGGGCGGGCCATCTCCTGTCTCT11.5384615384615385No Hit
GTGTAGGTGTAGTCGTTGATCTTGGTTGGGTCGTTCGGGTCGAAGGTGAA11.5384615384615385No Hit
GTTGAGTTGTGGTAGTTAAATTGTAATCATTATTAGTAGTAAGGCTCGGA11.5384615384615385No Hit
TATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTGGT11.5384615384615385No Hit
CATACCAGGCGTGGCGGGTTCGAGTAGGGGCTGCTTTTATCTCTGGCCTC11.5384615384615385No Hit
CTTAATACACATCTGAGGCTGCCGCCGAAGTTCGAGGTGGGGATCTCGGT11.5384615384615385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTG50.044.09
TGTGAGT50.044.012
AGGGGAA50.044.038
GTGTTGT50.044.08
TAAATCA50.044.020
GAGTGTA50.044.015
AAATCAG50.044.021
GCGATGA50.044.029
AGTAGGG50.044.035
AATCAGT50.044.022
GCCTAGG50.044.01
GTGTAAA50.044.017
TGCGATG50.044.028
AGGGTGT50.044.05
AAGGGAG50.044.043
GTGAGTG50.044.013
GATGAGT50.044.031
CTAGGGT50.044.03
AGGGAGC50.044.044
ATCAGTG50.044.023