FastQCFastQC Report
Wed 25 May 2016
SRR1780655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG23.076923076923077Illumina PCR Primer Index 3 (96% over 28bp)
CCACGTAGGTGATCTCGGCGCGCCGGGTGACGGGGGCGGCGCCGAAGCTG11.5384615384615385No Hit
GCCGGTGGTGGGGCGCAGTCGGACGCCGATCGGTCCGGGGCCTGTCTCTT11.5384615384615385No Hit
ATACACATCTCCGAGCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTC11.5384615384615385RNA PCR Primer, Index 14 (95% over 22bp)
CTGCTGGCTTGGATAGCGCGGGCCCACAGGATGCCCGAGAGGTCAGCCCA11.5384615384615385No Hit
CTTATACACATCTCCGACCCACGAGACCTCATGGGATCTCGTATGCCGTC11.5384615384615385No Hit
CGCCTGAACGCAGGCACATACTTCCTATTCTACACCCTAGTAGGCTCCCT11.5384615384615385No Hit
ACTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTTCTGCTTG11.5384615384615385RNA PCR Primer, Index 36 (100% over 29bp)
TGTGGAAGCTGTCGCGCTGGGAGGCGTCGACGGTAATGCCTGTCTCTTAT11.5384615384615385No Hit
CTCGGGGAGGACCACATCGGCGACGATCATGTCGCGCTCGTCCTGGGGGA11.5384615384615385No Hit
ATAGGATTTAGCCTATAATTTAACTTTGACAAAGTTATGAAATGGTTTTT11.5384615384615385No Hit
CGCCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGCTGT11.5384615384615385No Hit
CGCCAGGACGATGATCTTGTCGACGGCCTTGAGCTCCTGTCTCTTATACA11.5384615384615385No Hit
GACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTGGCCACCAATG11.5384615384615385No Hit
ACGCCGGTGCGCAGGGGCCGGCGGAGGTCGCCCGGCACCGGGTCTGTCGC11.5384615384615385No Hit
ATCTCAGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTT11.5384615384615385RNA PCR Primer, Index 36 (96% over 27bp)
TTTTCGCTTCCCACAGGTCTCCGAGCCCACGAGACGGTAAACCATCTCGT11.5384615384615385No Hit
CTTATACACATGTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT11.5384615384615385No Hit
CAGTCAAGCCGCGCTCTCCGAGGTCCCGCATCATCCGTCGTGTCGTCATC11.5384615384615385No Hit
GAGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCTGTCTCTTATA11.5384615384615385No Hit
CCCCAGCTCAGCCCACAGAGGCCCAGAGAGGAGCCACTGGCAGTCTACGG11.5384615384615385No Hit
CGCCGGACGCGTCGGCGCGGGCGGCCGACGTCGTGCGCTGTCTCTTATAC11.5384615384615385No Hit
CATCAAAACCCCCTCCCCATGCTTACAAGCAAGTACAGCAATCAACCCTC11.5384615384615385No Hit
GGCCAGCACGGACGGGATGATGCGCTCCACGAGGGGGCGCACGAAGCCGC11.5384615384615385No Hit
GAGATGGCCCGCCCCGGCTCGAGCGAGATGCGCGGGCACGCCCTGTCTCT11.5384615384615385No Hit
TAGACAACTGTGATACTTTGAAAGTCACGGTAACTTTTTCCTGCTAATAT11.5384615384615385No Hit
CTACCTAAAACTCACAGCCCTCGCTGTCACTTTCCTAGGACTTCTAACAG11.5384615384615385No Hit
AGACACATATAGATACACACTCCGAGCCCACGAGACTAACAACAATCTCG11.5384615384615385No Hit
ACCCGGCCGAGGAGGCCCGATGACCACCGTGGATCCGCAGCCGCCCCGCC11.5384615384615385No Hit
CCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGGCAAGCCAAC11.5384615384615385No Hit
GAACTGAAGACTTGGAATGGATCCAAGAGTACCGTTTCCCACTGCACGTC11.5384615384615385No Hit
GGTTCACGGTGATCAGCACCGATGCCTCGATGGAGGTGCCCCTGTCTCTT11.5384615384615385No Hit
ATCCGGTACTACACGTTCACCTTCGACCCGAACGACCCAACCAAGATCAA11.5384615384615385No Hit
AAACAAGACCAAATATTATGAAAATGATAGTCGTGATCCGCAATCATTTT11.5384615384615385No Hit
ATCTCCGAGCCCAGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTTG11.5384615384615385Illumina PCR Primer Index 6 (96% over 29bp)
CCGCAGGACGTCGCGCAGGGACGCCGTGTCGTCCCGGGCGGCCTCCCCCG11.5384615384615385No Hit
CCCCATCATACTCTTTCACCCACAGCACCAATCCTACCTCCATCGCTAAC11.5384615384615385No Hit
GATCGGACCGTGTCAGATGACGAACCGCAGCTTCGGTCGGACGGTCGAGG11.5384615384615385No Hit
CTGCAGTTCTCGTTCCGAGACCCCGACGGCTACGTCGTCACCATCCACGC11.5384615384615385No Hit
CCTCATGCCGGACGTGCTGACCGCCCTCCACGAGGCCGGCGTCCCGGCCA11.5384615384615385No Hit
CAGACTCCAAAGACCACATCATCGAAACCGCAAACATATCATACACATCT11.5384615384615385No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGCTGT11.5384615384615385No Hit
AAAAAACCCTTCAAAAAATCAGTGAATCCAGGAGCTGTTTTTTTTGAAAA11.5384615384615385No Hit
GCGTGAGCTCGTCGACGCGGCCATGTGGGCGGGGAACCTGTCTCTTATAC11.5384615384615385No Hit
GCACATGGTGCACCTGGATGTGAAGAAGATCGGGAAGATCCCAGACCTGT11.5384615384615385No Hit
TCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTTCTGCTTG11.5384615384615385RNA PCR Primer, Index 36 (96% over 30bp)
AATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACAC11.5384615384615385No Hit
GCCTTGTAGTCCTTGTTGTCCGAGCCCACGAGACAAACGGTCATCTCGTA11.5384615384615385No Hit
ATCATACTCTTTCACCCACAGCACCAATCCTACCTCCATCGCTAACCCCA11.5384615384615385No Hit
ATTTGGTATATGATTGAGATGGGGGCTAGTTTTTGTCATGTGAGAAGAAG11.5384615384615385No Hit
CTCCAGGAACTCGGGGCGGACCTGCGCTGCGTACTTCTGCAGCTGTCTCT11.5384615384615385No Hit
GTCTCCGGCTCCGGCCCCACCCTGCTCTTCGTGGCGCAGGACGAGGCCTC11.5384615384615385No Hit
CTTATACACATCTCCGACCCACGAGACTAACAACAATCTCGTATGCCGTC11.5384615384615385No Hit
ACCGCAAGTCGCTGCTCTCCGACGTCACCCGGGTCCTCTCCGAGAACCAC11.5384615384615385No Hit
GCCCAGGACCTGCTCCACGGAGCGCAGCCCGGTGCCCACGATGCGCACTG11.5384615384615385No Hit
AACGGTGATTTTAATTGATTTGTGACCTGTCTCCGAGCCCACGAGACTAA11.5384615384615385No Hit
GAGAAATAGCTCTCCCATCAAGTAACTTTTTGTAAGTGGTAAGTGCTGTC11.5384615384615385No Hit
GCCCCGCGGCGTGCAGCAGCGGTTCGACATGCCTCCAGGCTGTCTCTTAT11.5384615384615385No Hit
GGCCAGGGCGGTCCGCTCGGGCGCGGTCGCCAGCGCGGCCCCCCATGCCT11.5384615384615385No Hit
GGCTACGCCCGCGTCCTGATCAACCACGTCGACGACCCTGACGCGGCCGC11.5384615384615385No Hit
CTTTTATTCTAGCCACGGTGGCAGCTGATTAGATTGTGCCCACCCAGATT11.5384615384615385No Hit
CTGTCTCTTATACACATCTCCGAGACCACGAGACAAACGGTCATCTCGTA11.5384615384615385No Hit
ATCTCCACGAGCGCGATGCCGGGCGCGGAGTGCGTGGCGCTCACGAGCTC11.5384615384615385No Hit
CGCCACGGACGAGGCGCGCGCCGAGCACCTGGTGCACCTGCGGGCGCCCT11.5384615384615385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACC50.044.030
AACGCAG50.044.07
CCTATTC50.044.024
GGCACAT50.044.013
CACATAC50.044.015
TCCTATT50.044.023
CGCCTGA50.044.01
AGTAGGC50.044.039
ACGCAGG50.044.08
GCAGGCA50.044.010
GAACGCA50.044.06
ACACCCT50.044.032
CCTGAAC50.044.03
TCTACAC50.044.029
TACACCC50.044.031
ACTTCCT50.044.020
TTCCTAT50.044.022
CACCCTA50.044.033
CTGAACG50.044.04
TAGTAGG50.044.038