FastQCFastQC Report
Wed 25 May 2016
SRR1780654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64110
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTC150.002215894669.99531641
ACGCCCC150.002215894669.99531633
ATCTATA150.002215894669.99531623
TAACGCT150.002215894669.99531645
CCGTCCT150.002215894669.99531652
AGTGCTT150.002215894669.99531634
CCTAAAG252.3747704E-455.9962542
AATAGAG252.3747704E-455.9962545
AGACTAT200.006924107752.4964970
CAAACGC200.006924107752.4964930
CGAGTGA200.006924107752.4964964
TTCGTCT200.006924107752.4964942
AGGGTGC200.006924107752.4964924
CTAAAGC200.006924107752.496493
AACGCTC200.006924107752.4964946
CTTGAAT200.006924107752.4964938
GTACTCT200.006924107752.4964953
ACGCTCC200.006924107752.4964947
TCGAGTG200.006924107752.4964963
TAGGCGG200.006924107752.4964966
Produced by FastQC (version 0.11.5)