FastQCFastQC Report
Wed 25 May 2016
SRR1780654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64110
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT5960.9296521603493995No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2970.4632662611137108Illumina PCR Primer Index 9 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1840.28700670722196225No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC730.11386679145219156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGC150.002215306570.000013
GTACAAG150.002215306570.000011
GACTAGA150.002215306570.0000138
ACGCGAC200.00692227852.566
GTCATTG200.00692227852.549
CTTGTAG200.00692227852.548
AGTCACT200.00692227852.58
AGTCACA200.00692227852.510
GACACTG200.00692227852.55
CGTCTTG200.00692227852.527
TGAGTCT200.00692227852.558
CGCGACT200.00692227852.567
GCTGAGG200.00692227852.58
CTGGTCA200.00692227852.517
TACATAT200.00692227852.532
GTCACTC200.00692227852.59
TGACACT200.00692227852.54
GGTATTG200.00692227852.533
GCAGGGG200.00692227852.547
TTAGTCG200.00692227852.567
Produced by FastQC (version 0.11.5)