##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780654_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64110 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.09689596006863 34.0 34.0 34.0 32.0 34.0 2 33.39976602714085 34.0 34.0 34.0 33.0 34.0 3 33.5563094681017 34.0 34.0 34.0 34.0 34.0 4 33.56195601310248 34.0 34.0 34.0 34.0 34.0 5 33.54673217906723 34.0 34.0 34.0 34.0 34.0 6 37.30274528154734 38.0 38.0 38.0 36.0 38.0 7 36.91821868663235 38.0 37.0 38.0 35.0 38.0 8 36.95532678209328 38.0 37.0 38.0 35.0 38.0 9 36.769661519263764 38.0 37.0 38.0 34.0 38.0 10-11 36.494018093901104 38.0 37.0 38.0 34.0 38.0 12-13 36.40293245983466 38.0 37.0 38.0 34.0 38.0 14-15 36.62793635938231 38.0 37.0 38.0 34.0 38.0 16-17 36.73531430354079 38.0 37.0 38.0 34.5 38.0 18-19 36.75067852129153 38.0 37.0 38.0 34.0 38.0 20-21 36.49500077990953 38.0 37.0 38.0 34.0 38.0 22-23 36.574208391826545 38.0 37.0 38.0 34.0 38.0 24-25 35.93774762127593 38.0 36.5 38.0 32.5 38.0 26-27 36.17010606769615 38.0 37.0 38.0 34.0 38.0 28-29 36.07041023241304 38.0 37.0 38.0 33.5 38.0 30-31 35.80147402901264 38.0 36.5 38.0 32.0 38.0 32-33 36.272118234284825 38.0 37.0 38.0 34.0 38.0 34-35 36.32321010762752 38.0 37.0 38.0 34.0 38.0 36-37 36.431937295273755 38.0 37.0 38.0 34.0 38.0 38-39 36.08768522851349 38.0 37.0 38.0 32.5 38.0 40-41 35.6496724379972 38.0 36.0 38.0 31.5 38.0 42-43 36.020675401653406 38.0 36.5 38.0 32.5 38.0 44-45 36.18936983309936 38.0 37.0 38.0 34.0 38.0 46-47 36.49040711277492 38.0 37.0 38.0 34.0 38.0 48-49 36.29599126501326 38.0 37.0 38.0 34.0 38.0 50-51 36.41061456871003 38.0 37.0 38.0 34.0 38.0 52-53 36.360801746997346 38.0 37.0 38.0 34.0 38.0 54-55 36.516994228669475 38.0 37.0 38.0 34.0 38.0 56-57 36.55383715489003 38.0 37.0 38.0 34.0 38.0 58-59 36.64054749649041 38.0 37.0 38.0 34.0 38.0 60-61 36.3130868819217 38.0 37.0 38.0 34.0 38.0 62-63 36.57471533302137 38.0 37.0 38.0 34.0 38.0 64-65 36.57888004991421 38.0 37.0 38.0 34.0 38.0 66-67 36.01649508656996 38.0 37.0 38.0 33.0 38.0 68-69 35.49060209015754 38.0 36.5 38.0 31.0 38.0 70-71 35.527476212759325 38.0 36.0 38.0 31.0 38.0 72-73 35.300288566526284 38.0 36.0 38.0 29.0 38.0 74-75 35.87143971299329 38.0 37.0 38.0 32.5 38.0 76 35.33829355794728 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18589765928466306 1101 2 0.37988352588445196 1101 3 0.09632302058077613 1101 4 0.3238284486151457 1101 5 0.2557108464861315 1101 6 -0.7076705594827573 1101 7 0.06407462899785799 1101 8 0.3564994524567453 1101 9 0.27376059047314527 1101 10-11 0.07390531553439672 1101 12-13 0.15413873746334872 1101 14-15 0.5753934114140691 1101 16-17 -0.3888042393197111 1101 18-19 -0.5570638807614259 1101 20-21 0.15322251459935643 1101 22-23 0.5489384520942124 1101 24-25 -1.8642755563968691 1101 26-27 -0.5344273109898552 1101 28-29 0.10244942493184794 1101 30-31 0.8297206590791717 1101 32-33 0.11193946018739354 1101 34-35 0.17201342946110287 1101 36-37 0.011292791217137221 1101 38-39 0.2198279364096578 1101 40-41 0.7735135704054912 1101 42-43 0.42185013194862364 1101 44-45 0.39268483114940267 1101 46-47 0.4902869918158643 1101 48-49 -1.910901090445087 1101 50-51 -1.4828251970922892 1101 52-53 -2.229189371221686 1101 54-55 -2.0919687689026176 1101 56-57 -1.5610788891560148 1101 58-59 -0.6706243730448307 1101 60-61 -1.9604406810737558 1101 62-63 -1.3368131357963549 1101 64-65 -1.4168174874426214 1101 66-67 -2.360391197423148 1101 68-69 -1.30818351173383 1101 70-71 -1.5104939797326153 1101 72-73 -2.698407333810543 1101 74-75 -0.992818427491855 1101 76 -1.8231519683438222 1102 1 -0.45409746920151406 1102 2 0.1565246171035497 1102 3 0.12628283059173384 1102 4 -0.0011421193221679005 1102 5 0.10396319090215655 1102 6 0.30527954809650737 1102 7 0.051327501161210876 1102 8 -0.04515686224304716 1102 9 0.44702409877501736 1102 10-11 0.43843663530868326 1102 12-13 0.5131084122898031 1102 14-15 -0.17559104033765038 1102 16-17 0.25399200961464885 1102 18-19 -0.3683739120203029 1102 20-21 0.5898319409786055 1102 22-23 0.7120732712392552 1102 24-25 -4.581829244538795 1102 26-27 -1.1921222730326448 1102 28-29 0.2579319077800548 1102 30-31 1.3406815651807449 1102 32-33 0.026383498672352346 1102 34-35 0.7015875779610923 1102 36-37 -2.7081080125826418 1102 38-39 0.11366953101851607 1102 40-41 0.8981837283237368 1102 42-43 0.5450385377457323 1102 44-45 0.6480130493932208 1102 46-47 0.9914033804002855 1102 48-49 -3.5945165656206655 1102 50-51 -1.0215943279330375 1102 52-53 -0.06457279995550635 1102 54-55 0.24438896259577803 1102 56-57 0.33217406419923634 1102 58-59 0.17113261960730597 1102 60-61 -6.343301071606781 1102 62-63 -2.5430202563257254 1102 64-65 -0.30826392108853895 1102 66-67 1.0677133777402545 1102 68-69 -1.2105299575585349 1102 70-71 -0.9346607478891542 1102 72-73 -5.6115725999169825 1102 74-75 -1.6137943540943809 1102 76 -0.0894207947149468 1103 1 0.7112154787201277 1103 2 0.5084118602951406 1103 3 0.11409936188653091 1103 4 0.29890565898647736 1103 5 0.32541565148505214 1103 6 0.16146683906640646 1103 7 0.821274945021699 1103 8 0.6385507345080228 1103 9 0.3540952884549 1103 10-11 0.2390816424754334 1103 12-13 -0.5934372159949532 1103 14-15 -0.3499818669023824 1103 16-17 0.036481259495197094 1103 18-19 0.3419700918019615 1103 20-21 -0.5641336586391645 1103 22-23 0.349197017058664 1103 24-25 -2.949961112670664 1103 26-27 -0.6290549056174513 1103 28-29 0.25900691972463363 1103 30-31 0.7975616416260323 1103 32-33 1.0281751852466456 1103 34-35 0.22117668450671601 1103 36-37 -0.8224176160226762 1103 38-39 -0.2174341479112485 1103 40-41 -0.05129071028114396 1103 42-43 -0.12576891567042026 1103 44-45 0.9535553331963769 1103 46-47 0.7620674988904881 1103 48-49 -2.3492943964854547 1103 50-51 -0.6435484607567403 1103 52-53 0.3323454991367072 1103 54-55 -0.477302389530351 1103 56-57 0.2693455883272833 1103 58-59 -0.4816385222942614 1103 60-61 -3.5185280185728587 1103 62-63 -0.9805501177686295 1103 64-65 0.23760325521341485 1103 66-67 -0.09986903984216156 1103 68-69 0.11245140890108729 1103 70-71 0.17224321771045936 1103 72-73 -4.765418753763136 1103 74-75 -2.1637226866314094 1103 76 -2.3727461649968404 1104 1 0.23956712707177275 1104 2 0.16621405809733858 1104 3 0.25497848276565094 1104 4 -0.13406137398055762 1104 5 -0.3196392935698924 1104 6 -0.11062107302150537 1104 7 0.34325296699972085 1104 8 0.4003089762662668 1104 9 0.8321172664768781 1104 10-11 -0.7474018740080837 1104 12-13 -0.059810842368577255 1104 14-15 0.43111703419651803 1104 16-17 0.1717559847699235 1104 18-19 0.6574645972964674 1104 20-21 -0.04644135094686419 1104 22-23 0.12809811595975162 1104 24-25 0.2010278983183511 1104 26-27 0.71446157789903 1104 28-29 1.087248677966393 1104 30-31 0.4203088943732851 1104 32-33 0.161911448982913 1104 34-35 -0.332449689119656 1104 36-37 0.9297801861751225 1104 38-39 1.2098185993414958 1104 40-41 1.2589290699386382 1104 42-43 0.3885167986152922 1104 44-45 0.5550937947348373 1104 46-47 0.6895400263630194 1104 48-49 -0.18544824263930337 1104 50-51 0.12337461616634471 1104 52-53 0.1201476969389077 1104 54-55 0.11830200607404606 1104 56-57 -0.15614891716721502 1104 58-59 -0.9452648959206442 1104 60-61 0.1266368165919758 1104 62-63 0.09076856355004992 1104 64-65 0.39210874971890775 1104 66-67 -0.2037151936883177 1104 68-69 0.5524514089010921 1104 70-71 0.7242212396884895 1104 72-73 0.519855971511582 1104 74-75 -0.2817446646533881 1104 76 -0.46109781334848776 1105 1 0.4512269123731798 1105 2 0.3399877988074991 1105 3 0.10822373462361412 1105 4 -0.07675336407139355 1105 5 -0.5828928030661729 1105 6 0.03633185844010001 1105 7 0.6529144038287882 1105 8 -0.27741191555867317 1105 9 0.6537522788633368 1105 10-11 0.18358078495145236 1105 12-13 -1.1219578283305935 1105 14-15 -0.0013189691065349507 1105 16-17 0.20498472135126633 1105 18-19 0.251882433795231 1105 20-21 0.024524792736045242 1105 22-23 0.05420876831318111 1105 24-25 -0.023612894414931418 1105 26-27 0.11168701142504744 1105 28-29 0.5971413286682932 1105 30-31 0.8060892411940941 1105 32-33 0.8623078156220529 1105 34-35 -1.2143819764854697 1105 36-37 0.057195545382384694 1105 38-39 0.007522975889806105 1105 40-41 0.29767390891469603 1105 42-43 -0.33633869271418604 1105 44-45 0.7164975932484694 1105 46-47 0.8295895721929156 1105 48-49 -0.04041108326688203 1105 50-51 0.33737957074932723 1105 52-53 -0.25948896697521207 1105 54-55 0.18337219599972343 1105 56-57 0.001258184401734752 1105 58-59 -0.3971724103715104 1105 60-61 0.6574708380618404 1105 62-63 -0.30645686997596755 1105 64-65 0.4744704342771229 1105 66-67 0.027250949994588325 1105 68-69 -0.2327178726843684 1105 70-71 0.7035110827933551 1105 72-73 0.21749428695337514 1105 74-75 -0.33497340123472696 1105 76 0.4215116003591888 1106 1 -0.7482495845185184 1106 2 0.21340183887002695 1106 3 0.29203149264884587 1106 4 0.3207454544111812 1106 5 0.1876383344624557 1106 6 -0.007584235645040849 1106 7 -1.1374298721736835 1106 8 0.42568184239384976 1106 9 -0.3053490408007491 1106 10-11 0.3122207675013513 1106 12-13 1.1193625628425536 1106 14-15 -0.512225283862783 1106 16-17 0.2628790755156558 1106 18-19 0.26862362694606645 1106 20-21 0.24737715758863033 1106 22-23 0.481790838331726 1106 24-25 -0.326663027108431 1106 26-27 0.40792069739588754 1106 28-29 0.3305909960256912 1106 30-31 0.4512522905997045 1106 32-33 -0.27929250429651375 1106 34-35 0.6040642372055913 1106 36-37 0.8973453254383728 1106 38-39 0.014993801497830361 1106 40-41 0.6810854041704388 1106 42-43 0.07593818226309423 1106 44-45 0.6612752861993485 1106 46-47 0.8236819850332822 1106 48-49 1.139906654036352 1106 50-51 0.3037699441088435 1106 52-53 0.6741279305417791 1106 54-55 -0.23982917092506284 1106 56-57 0.13536950152101213 1106 58-59 0.36957787137495757 1106 60-61 0.7063493053610728 1106 62-63 0.7589446641789834 1106 64-65 -0.5985291658246652 1106 66-67 0.3035624343440304 1106 68-69 1.1079590459181574 1106 70-71 0.2609328299849807 1106 72-73 0.7559026920866145 1106 74-75 -0.21362247238025134 1106 76 0.44161557389320194 1107 1 0.8074001928823691 1107 2 -0.033753839173925826 1107 3 0.3088147588491168 1107 4 0.009919364382206197 1107 5 0.1540364688699185 1107 6 0.25098406341509616 1107 7 0.7766398048809435 1107 8 0.1467616247413872 1107 9 -0.053918046524728425 1107 10-11 0.6270925080143925 1107 12-13 0.09680231975019638 1107 14-15 0.3536852524950689 1107 16-17 0.7890433041920772 1107 18-19 0.7178498300412528 1107 20-21 0.8486950856502489 1107 22-23 -0.07316994182515657 1107 24-25 0.3009957955896212 1107 26-27 -0.5135159501908575 1107 28-29 0.5545681001172795 1107 30-31 0.5840167595418251 1107 32-33 0.9540302290792226 1107 34-35 0.05581676352881715 1107 36-37 1.0684639742971171 1107 38-39 0.5707495784747181 1107 40-41 0.7136802737909633 1107 42-43 0.8918875851321459 1107 44-45 0.7341146615085208 1107 46-47 0.6595239749986561 1107 48-49 0.9598487076014734 1107 50-51 0.8787999773220392 1107 52-53 0.6441123839373049 1107 54-55 0.7032137235700304 1107 56-57 0.8657772465566964 1107 58-59 0.397544092843404 1107 60-61 0.8724153077637311 1107 62-63 0.8636899118646539 1107 64-65 0.882991574759032 1107 66-67 0.5115336024593589 1107 68-69 1.2308302211001276 1107 70-71 0.48194194594852036 1107 72-73 1.6095349442242721 1107 74-75 -0.056672433513739406 1107 76 1.3167834065552029 1108 1 -0.579873866717044 1108 2 0.42282896492963573 1108 3 0.18295984922527708 1108 4 0.2892305514852822 1108 5 0.04418058220650778 1108 6 -0.332919209667466 1108 7 -0.11637436219282193 1108 8 -0.43335562000702765 1108 9 -0.7338765223429959 1108 10-11 0.4209676912093059 1108 12-13 0.7860121389978758 1108 14-15 0.44824436338906537 1108 16-17 -0.028616686037530314 1108 18-19 0.2783652184144785 1108 20-21 0.8285741770034463 1108 22-23 0.18257016564920292 1108 24-25 1.1840869045295292 1108 26-27 -0.33032103079662534 1108 28-29 -0.2711829990522361 1108 30-31 0.2948741117645852 1108 32-33 0.7433866042624189 1108 34-35 0.4682801245449326 1108 36-37 1.1338950930074247 1108 38-39 1.2708900279129267 1108 40-41 1.1547831072057235 1108 42-43 -0.444091897036877 1108 44-45 0.8327956580888767 1108 46-47 0.9529717033816496 1108 48-49 1.1519896455594605 1108 50-51 0.8646634360421146 1108 52-53 0.31741477768424176 1108 54-55 0.13375231663304987 1108 56-57 0.6602020145097995 1108 58-59 0.5007133649489219 1108 60-61 1.1605809159708542 1108 62-63 0.9725076939848307 1108 64-65 0.8303230354331959 1108 66-67 0.8204307690445987 1108 68-69 1.6869855703917764 1108 70-71 -0.02782844975250498 1108 72-73 0.500228642319037 1108 74-75 -0.26713286341114895 1108 76 1.31275311832853 1109 1 -0.22809521059055982 1109 2 0.32228548666876833 1109 3 0.2276732879604566 1109 4 0.21561395069476674 1109 5 0.07678927785867984 1109 6 -0.05500418990462208 1109 7 -0.04473404598729047 1109 8 0.5572570282143161 1109 9 0.7834159677755821 1109 10-11 0.3731663078100951 1109 12-13 0.5943125342547972 1109 14-15 0.7288767744562534 1109 16-17 0.6781520886660317 1109 18-19 0.703389921971791 1109 20-21 0.7581690386635316 1109 22-23 0.4797539601156018 1109 24-25 0.3057519242923732 1109 26-27 0.5561661233535773 1109 28-29 0.12073893770665478 1109 30-31 0.8684907125551007 1109 32-33 0.75376209833356 1109 34-35 0.2302613498413848 1109 36-37 1.1072152511101905 1109 38-39 0.8873673006402001 1109 40-41 1.1546595894191611 1109 42-43 1.2618122531607483 1109 44-45 0.7527561323971739 1109 46-47 0.5951406757136652 1109 48-49 0.6040647443736873 1109 50-51 0.2113778629195835 1109 52-53 0.018378216419428384 1109 54-55 -0.21765578613374714 1109 56-57 -0.0019768392451453565 1109 58-59 -0.017196714102453825 1109 60-61 0.7805166867218531 1109 62-63 0.07292765445914284 1109 64-65 0.6115730354331959 1109 66-67 0.8177133777402545 1109 68-69 0.23379881149849524 1109 70-71 0.2741725383897844 1109 72-73 -0.14119922329360435 1109 74-75 -0.20302713218585922 1109 76 0.7670515373008584 1110 1 -0.9527252317322095 1110 2 -0.7431214900754171 1110 3 -0.03329394248351747 1110 4 0.14716786189984532 1110 5 0.2948125336726335 1110 6 -0.31623569096170456 1110 7 0.35405030919240943 1110 8 0.540607979588529 1110 9 0.8885956717924941 1110 10-11 -0.7076344321476213 1110 12-13 0.6007871642759355 1110 14-15 0.7089243431666148 1110 16-17 0.381772596065602 1110 18-19 0.09497290294431338 1110 20-21 0.7364988483886918 1110 22-23 -0.36710121958841313 1110 24-25 -0.4838226000205239 1110 26-27 0.7643452988292623 1110 28-29 0.4640925317869957 1110 30-31 -0.09213296609182464 1110 32-33 0.3189048044646441 1110 34-35 0.5157396796511051 1110 36-37 0.9519562659093452 1110 38-39 0.5223435162850194 1110 40-41 -0.9035460131178397 1110 42-43 0.35886563582459985 1110 44-45 0.5006419674923208 1110 46-47 0.2990915213796299 1110 48-49 1.1351165414138578 1110 50-51 -0.3153795366575807 1110 52-53 0.4209284278359178 1110 54-55 0.2600794147119103 1110 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2116 38-39 -3.3410111696918676 2116 40-41 -0.9936971062803508 2116 42-43 -0.9923215247379957 2116 44-45 -0.5156162138194773 2116 46-47 -0.9247456879226945 2116 48-49 -0.6413421690721535 2116 50-51 -0.9778743145436621 2116 52-53 0.07698259856422851 2116 54-55 -0.43242511112013915 2116 56-57 -0.41707278371983136 2116 58-59 -2.207061303106272 2116 60-61 -1.0839192142035756 2116 62-63 0.16034940187339686 2116 64-65 -2.054033251991953 2116 66-67 -0.9352806342357951 2116 68-69 -1.272920704015938 2116 70-71 0.6713384338715542 2116 72-73 0.14120755406077734 2116 74-75 0.08152308556046961 2116 76 -1.2289677876855265 2117 1 0.6177426864521536 2117 2 0.4299662095603338 2117 3 0.2625172090995562 2117 4 -0.06108374919570281 2117 5 0.030102530870728117 2117 6 0.3345768615740212 2117 7 0.5756109704420638 2117 8 0.5773829865932925 2117 9 -0.31160046157819465 2117 10-11 -0.33764283786350546 2117 12-13 -0.7232531831603239 2117 14-15 0.2585180668987519 2117 16-17 -0.44349358493747104 2117 18-19 -0.738593922496996 2117 20-21 -0.01633808072310927 2117 22-23 -0.10697933670805782 2117 24-25 -0.8886278676024801 2117 26-27 -1.4798757714985555 2117 28-29 -0.8317186197960851 2117 30-31 0.19187515943352906 2117 32-33 -1.0157477592787032 2117 34-35 0.4305967824810324 2117 36-37 -0.6097206744100774 2117 38-39 0.28971532911774744 2117 40-41 -0.10755974245378752 2117 42-43 0.7391192082538467 2117 44-45 -0.002720318862408533 2117 46-47 -0.44884207346485994 2117 48-49 -0.369716745221055 2117 50-51 0.03965688811126711 2117 52-53 0.4466709327392451 2117 54-55 0.062019734129123094 2117 56-57 0.541389809166688 2117 58-59 -0.870325136884496 2117 60-61 -0.13689158271955648 2117 62-63 -0.49802661717284735 2117 64-65 -0.940008289868004 2117 66-67 -0.5105998752717937 2117 68-69 0.3039316154415346 2117 70-71 -0.2945911527383629 2117 72-73 0.4873946978454953 2117 74-75 0.4430229773431691 2117 76 0.8618625997324045 2118 1 -0.19039158473664486 2118 2 0.2706236369577866 2118 3 -0.1056490977463227 2118 4 0.015732185061558823 2118 5 0.35352338190492105 2118 6 0.07679428618576623 2118 7 0.8552265425571122 2118 8 0.6416632289500015 2118 9 -0.8953806691135426 2118 10-11 -0.30774869481732736 2118 12-13 -0.4514375971167226 2118 14-15 0.657640568465709 2118 16-17 -2.3730499607296593 2118 18-19 -0.3030308610024619 2118 20-21 0.229727104874776 2118 22-23 0.7854226411455514 2118 24-25 0.8839015564521233 2118 26-27 -2.1368679018697563 2118 28-29 -0.015575434337485206 2118 30-31 -0.1736795449330728 2118 32-33 -0.04520663524500179 2118 34-35 -0.061202785734025156 2118 36-37 -2.390781333230329 2118 38-39 -0.10808396052637903 2118 40-41 -0.19943591767490432 2118 42-43 0.6289792263609613 2118 44-45 -1.991594892300668 2118 46-47 0.17930055485187069 2118 48-49 0.4162363155770521 2118 50-51 -0.5007707182680079 2118 52-53 -1.1063097762237746 2118 54-55 0.018632311606658902 2118 56-57 0.7931656988525972 2118 58-59 -0.3133573814697712 2118 60-61 -0.7124713584699549 2118 62-63 -0.37582103182567295 2118 64-65 0.5120426886123894 2118 66-67 0.1948810077980596 2118 68-69 -0.41768897095027313 2118 70-71 0.07923362614595675 2118 72-73 0.9891705875314116 2118 74-75 0.3124254426959112 2118 76 0.27700292983895736 2119 1 0.6751817450119759 2119 2 -0.551261024959139 2119 3 0.18763629007462157 2119 4 -0.3237623706582937 2119 5 -0.11216421051341285 2119 6 -0.4092589467756582 2119 7 -0.2796706210401467 2119 8 0.26735216563503883 2119 9 0.028130555513428135 2119 10-11 -0.2948437344731971 2119 12-13 -0.2771198124682499 2119 14-15 -0.296889610321756 2119 16-17 -0.10950647370183475 2119 18-19 -0.0038643063580181547 2119 20-21 -0.9117569655647983 2119 22-23 -0.05314773121632044 2119 24-25 1.2022239116073834 2119 26-27 0.8021359965036794 2119 28-29 -0.1754423519339383 2119 30-31 0.8497274990244534 2119 32-33 0.5253001533018491 2119 34-35 0.5331815401162174 2119 36-37 -0.13234605967205226 2119 38-39 -0.5823076465056758 2119 40-41 -0.589592524745747 2119 42-43 0.3297958683827389 2119 44-45 0.45413033958534044 2119 46-47 0.27292873068195433 2119 48-49 -0.22244159812102993 2119 50-51 0.5625274400866047 2119 52-53 0.13023075341731527 2119 54-55 0.22810267052587108 2119 56-57 -0.8794213424163928 2119 58-59 0.03426998780028612 2119 60-61 0.7560055873560998 2119 62-63 0.168791819364003 2119 64-65 0.26491605868901047 2119 66-67 0.29736454053094974 2119 68-69 0.649826824183485 2119 70-71 0.25111492739613084 2119 72-73 1.0055370346345072 2119 74-75 0.18009918916721546 2119 76 0.10597859861164238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 7.0 23 10.0 24 21.0 25 41.0 26 86.0 27 164.0 28 295.0 29 454.0 30 712.0 31 1252.0 32 2017.0 33 3121.0 34 4978.0 35 7982.0 36 14785.0 37 28184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.86180003119638 24.083606301669004 12.316331305568553 27.738262361566058 2 17.767898923724847 19.86429574169396 38.67883325534238 23.688972079238805 3 17.165808766183122 25.84620184058649 30.053033848073625 26.934955545156765 4 12.664170956169086 19.291842146311026 39.686476368741225 28.35751052877866 5 12.044922788956482 37.91296209639682 37.016066136328185 13.026048978318517 6 26.844486039619404 40.53345811885821 18.256122289814382 14.365933551708002 7 25.659023553267822 30.198097020745596 24.52971455311184 19.613164872874748 8 22.52846669786305 37.18452659491499 20.388394946186242 19.89861176103572 9 23.012010606769614 14.836998908126658 22.997972235220715 39.15301824988301 10-11 24.18031508345032 26.299329277803775 27.92232101076275 21.598034627983154 12-13 23.64217750740914 23.225705818125096 27.05194197473093 26.080174699734833 14-15 21.572297613476838 25.03119638121978 23.765403213227266 29.63110279207612 16-17 22.557323350491345 27.81937295273748 25.809546092653253 23.81375760411792 18-19 22.36234596786773 27.330369677117456 26.662767119014198 23.644517236000624 20-21 21.593355170800187 27.328809858056463 26.317267197005144 24.760567774138202 22-23 22.389642801435034 27.209483699890814 27.075339260645766 23.32553423802839 24-25 22.321790672282017 28.018249883013567 26.766495086569957 22.89346435813446 26-27 21.51692403681173 28.049446264233346 27.504289502417716 22.9293401965372 28-29 21.70566214319139 26.39681796911558 28.904227109655277 22.99329277803775 30-31 21.83512712525347 27.002807674309782 28.0985805646545 23.06348463578225 32-33 21.28919045390735 27.9293401965372 27.465294025892995 23.316175323662456 34-35 21.500545936671347 28.630478864451725 27.668850413352054 22.20012478552488 36-37 22.770238652316333 27.39276243955701 26.77195445328342 23.06504445484324 38-39 21.627671190141943 27.253938543128996 28.146934955545156 22.971455311183902 40-41 21.08875370457027 26.395258150054595 28.539229449383875 23.976758695991265 42-43 22.23366089533614 26.035719856496648 28.89018873810638 22.840430510060834 44-45 20.41257214163157 26.636250194977386 28.735766651068477 24.215411012322573 46-47 21.87724223990017 25.916393698330992 28.28575885197317 23.920605209795664 48-49 20.876618312275777 25.91093433161753 29.111683044766806 24.100764311339884 50-51 20.145843082202465 26.729059429106222 28.858212447356106 24.266885041335204 52-53 19.82686008423023 26.36406176883482 30.40945250350959 23.399625643425363 54-55 19.284822960536577 26.773514272344407 29.747309312119796 24.19435345499922 56-57 20.138043986897518 26.118390266729058 28.956481048198405 24.78708469817501 58-59 19.286382779597567 27.044142879425987 29.00951489627203 24.659959444704413 60-61 19.983621899859617 27.56980190297925 28.048666354702856 24.397909842458272 62-63 18.40274528154734 28.639837778817657 27.467633754484478 25.48978318515052 64-65 19.475120885977226 29.15379815941351 26.192481672126032 25.178599282483233 66-67 19.22165028856653 28.814537513648418 26.12228981438153 25.841522383403525 68-69 19.33473717048822 28.83949461862424 25.97722664170956 25.848541569177975 70-71 19.783185150522538 28.245983465917952 26.16596474808922 25.804866635470287 72-73 19.69427546404617 27.579940726875684 25.917953517391982 26.807830291686162 74-75 19.9859616284511 27.393542349087507 26.182342848229606 26.438153174231786 76 20.25892996412416 27.671190141943537 26.37030104507877 25.699578848853534 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.5 17 1.0 18 0.5 19 0.5 20 1.0 21 1.0 22 4.5 23 14.0 24 31.5 25 43.0 26 100.0 27 181.0 28 269.0 29 333.0 30 393.0 31 486.0 32 741.5 33 964.0 34 1064.5 35 1415.0 36 1837.0 37 2009.0 38 2218.5 39 3017.0 40 3899.5 41 4390.5 42 4588.0 43 4547.0 44 4720.5 45 5147.5 46 5360.0 47 5283.0 48 4901.0 49 4286.5 50 3977.0 51 3605.5 52 2889.0 53 2319.5 54 2095.0 55 1945.5 56 1483.5 57 1041.0 58 911.0 59 707.0 60 416.0 61 272.0 62 215.0 63 171.0 64 105.5 65 80.0 66 58.0 67 40.0 68 32.0 69 17.0 70 6.5 71 3.0 72 4.0 73 2.5 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 64110.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.778505693339575 #Duplication Level Percentage of deduplicated Percentage of total 1 59.1286957885656 23.520511620652005 2 15.116461454003607 12.026204960224614 3 8.234648262881343 9.82686008423023 4 4.9329464355736805 7.849009514896273 5 3.0664261626539098 6.098892528466697 6 2.2233550309779626 5.3065044454843235 7 1.5881107364128302 4.422087037903603 8 1.0705042741745747 3.406644829199813 9 0.89404752568426 3.2007487131492747 >10 3.7212767626068546 22.375604429886135 >50 0.011763783232687633 0.28700670722196225 >100 0.007842522155125087 0.750272968335673 >500 0.003921261077562544 0.9296521603493995 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 596 0.9296521603493995 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 297 0.4632662611137108 Illumina PCR Primer Index 9 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 184 0.28700670722196225 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 73 0.11386679145219156 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0031196381219778507 0.0 2 0.0 0.0 0.0 0.007799095304944627 0.0 3 0.0 0.0 0.0 0.009358914365933552 0.0 4 0.0 0.0 0.0 0.015598190609889253 0.0 5 0.0 0.0 0.0 0.015598190609889253 0.0 6 0.0 0.0 0.0 0.015598190609889253 0.0 7 0.0 0.0 0.0 0.015598190609889253 0.0 8 0.0 0.0 0.0 0.015598190609889253 0.0 9 0.0 0.0 0.0 0.02963656215878958 0.0 10 0.0 0.0 0.0 0.04523475276867883 0.0 11 0.0 0.0 0.0 0.04991420995164561 0.0 12 0.0 0.0 0.0 0.04991420995164561 0.0 13 0.0 0.0 0.0 0.04991420995164561 0.0 14 0.0 0.0 0.0 0.05459366713461238 0.0 15 0.0 0.0 0.0 0.06083294337856809 0.0 16 0.0 0.0 0.0 0.06083294337856809 0.0 17 0.0 0.0 0.0 0.07175167680549056 0.0 18 0.0 0.0 0.0 0.07643113398845734 0.0 19 0.0 0.0 0.0 0.08267041023241305 0.0 20 0.0 0.0 0.0 0.08734986741537981 0.0 21 0.0 0.0 0.0 0.09358914365933552 0.0 22 0.0 0.0 0.0 0.09982841990329122 0.0 23 0.0 0.0 0.0 0.10606769614724693 0.0 24 0.0 0.0 0.0 0.11230697239120262 0.0 25 0.0 0.0 0.0 0.11386679145219154 0.0 26 0.0 0.0 0.0 0.12010606769614725 0.0 27 0.0 0.0 0.0 0.13726407736702542 0.0 28 0.0 0.0 0.0 0.15754172515988146 0.0 29 0.0 0.0 0.0 0.17625955389174855 0.0 30 0.0 0.0 0.0 0.1918577445016378 0.0 31 0.0 0.0 0.0 0.2168148494774606 0.0 32 0.0 0.0 0.0 0.24957104975822805 0.0 33 0.0 0.0 0.0 0.30572453595382937 0.0 34 0.0 0.0 0.0 0.3509592887225082 0.0 35 0.0 0.0 0.0 0.4320698798939323 0.0 36 0.0 0.0 0.0 0.5802526906878802 0.0 37 0.0 0.0 0.0 0.8579004835439089 0.0 38 0.0 0.0 0.0 1.2260177819372953 0.0 39 0.0 0.0 0.0 1.7298393386367181 0.0 40 0.0 0.0 0.0 2.27889564810482 0.0 41 0.0 0.0 0.0 2.8872250818905005 0.0 42 0.0 0.0 0.0 3.5969427546404615 0.0 43 0.0 0.0 0.0 4.222430198097021 0.0 44 0.0 0.0 0.0 4.9508656995788485 0.0 45 0.0 0.0 0.0 5.627827172048042 0.0 46 0.0 0.0 0.0 6.384339416627671 0.0 47 0.0 0.0 0.0 7.086257994072688 0.0 48 0.0 0.0 0.0 7.799095304944626 0.0 49 0.0 0.0 0.0 8.462018405864919 0.0 50 0.0 0.0 0.0 9.04071127749181 0.0 51 0.0 0.0 0.0 9.745749493058804 0.0 52 0.0 0.0 0.0 10.382155669942287 0.0 53 0.0 0.0 0.0 11.076275152082358 0.0 54 0.0 0.0 0.0 11.63936983309936 0.0 55 0.0 0.0 0.0 12.29293401965372 0.0 56 0.0 0.0 0.0 12.921541101232258 0.0 57 0.0 0.0 0.0 13.504913430042116 0.0 58 0.0 0.0 0.0 14.025892996412416 0.0 59 0.0 0.0 0.0 14.587427858368429 0.0 60 0.0 0.0 0.0 15.113086881921697 0.0 61 0.0 0.0 0.0 15.60287006707222 0.0 62 0.0 0.0 0.0 16.141007643113397 0.0 63 0.0 0.0 0.0 16.7041023241304 0.0 64 0.0 0.0 0.0 17.147090937451257 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGGC 15 0.0022153065 70.00001 3 GTACAAG 15 0.0022153065 70.00001 1 GACTAGA 15 0.0022153065 70.00001 38 ACGCGAC 20 0.006922278 52.5 66 GTCATTG 20 0.006922278 52.5 49 CTTGTAG 20 0.006922278 52.5 48 AGTCACT 20 0.006922278 52.5 8 AGTCACA 20 0.006922278 52.5 10 GACACTG 20 0.006922278 52.5 5 CGTCTTG 20 0.006922278 52.5 27 TGAGTCT 20 0.006922278 52.5 58 CGCGACT 20 0.006922278 52.5 67 GCTGAGG 20 0.006922278 52.5 8 CTGGTCA 20 0.006922278 52.5 17 TACATAT 20 0.006922278 52.5 32 GTCACTC 20 0.006922278 52.5 9 TGACACT 20 0.006922278 52.5 4 GGTATTG 20 0.006922278 52.5 33 GCAGGGG 20 0.006922278 52.5 47 TTAGTCG 20 0.006922278 52.5 67 >>END_MODULE