FastQCFastQC Report
Wed 25 May 2016
SRR1780652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60286
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTC150.002215387369.9941911
TACATGC150.002215387369.994192
GTACATG200.006764674652.802381
ACATAGA200.006764674652.802381
TTAGGCG200.006922538452.4956443
TATTCCC200.006922538452.4956445
TTGCGCT200.006922538452.49564414
GTCAAAG200.006922538452.49564422
TTCGGTC200.006922538452.49564428
GATTGCG200.006922538452.49564412
TCGGTCA200.006922538452.49564429
TACCACT400.002402322834.99709740
AATCACT552.9615941E-431.8155427
GCTGTTA450.00427818331.10852818
AGGTGGC450.00427818331.1085288
AGATGTG450.00427818331.1085285
GAATTGA1202.2118911E-929.16424670
TTAGGGT500.007160014527.99767758
ACTTGTT500.007160014527.99767736
TCTTAGG500.007160014527.99767729
Produced by FastQC (version 0.11.5)