##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780652_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60286 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.60577911953024 34.0 31.0 34.0 12.0 34.0 2 30.92280131373785 34.0 32.0 34.0 23.0 34.0 3 31.318531665726702 34.0 33.0 34.0 23.0 34.0 4 31.684138937730154 34.0 34.0 34.0 23.0 34.0 5 31.327621670039477 34.0 33.0 34.0 23.0 34.0 6 34.491440798858775 38.0 35.0 38.0 26.0 38.0 7 33.63293301927479 37.0 34.0 38.0 21.0 38.0 8 32.665693527518826 37.0 32.0 38.0 11.0 38.0 9 33.408021762930034 37.0 34.0 38.0 21.0 38.0 10-11 33.86105397604751 37.0 34.0 38.0 22.5 38.0 12-13 34.23141359519623 38.0 34.5 38.0 24.5 38.0 14-15 33.80206847360913 37.0 34.0 38.0 22.0 38.0 16-17 33.64709053511595 37.0 34.0 38.0 21.0 38.0 18-19 33.66516272434728 37.0 34.0 38.0 22.0 38.0 20-21 33.68143515907508 37.0 34.0 38.0 21.5 38.0 22-23 33.72685200544073 37.0 34.0 38.0 22.0 38.0 24-25 34.1441379424742 37.5 34.5 38.0 23.5 38.0 26-27 34.222265534286564 38.0 34.5 38.0 23.5 38.0 28-29 34.4310951132933 38.0 35.5 38.0 24.5 38.0 30-31 34.273620741133925 38.0 35.0 38.0 24.0 38.0 32-33 33.983163586902435 37.0 34.0 38.0 22.5 38.0 34-35 33.847394088179676 37.0 34.0 38.0 23.0 38.0 36-37 33.83709318913181 37.0 34.0 38.0 22.5 38.0 38-39 34.382310984308134 38.0 35.0 38.0 24.5 38.0 40-41 33.8319925024052 37.5 34.0 38.0 22.0 38.0 42-43 34.2275652722025 37.0 34.0 38.0 24.0 38.0 44-45 33.18153468466974 37.0 34.0 38.0 21.0 38.0 46-47 33.928880668812 37.0 34.0 38.0 23.0 38.0 48-49 33.61077198686262 37.0 34.0 38.0 22.0 38.0 50-51 33.61198288159772 37.0 34.0 38.0 22.0 38.0 52-53 33.61389874929503 37.0 34.0 38.0 22.5 38.0 54-55 33.301902597618025 37.0 33.5 38.0 21.0 38.0 56-57 33.431675679262185 37.0 33.5 38.0 22.0 38.0 58-59 33.28174037089872 37.0 33.0 38.0 22.0 38.0 60-61 33.302690508575786 37.0 33.5 38.0 21.5 38.0 62-63 33.37260723882825 37.0 33.0 38.0 21.5 38.0 64-65 33.44594101449756 37.0 33.5 38.0 21.5 38.0 66-67 33.3589888199582 37.0 33.5 38.0 21.5 38.0 68-69 32.905392628470956 37.0 31.5 38.0 16.0 38.0 70-71 32.731654115383336 37.0 31.5 38.0 19.5 38.0 72-73 32.828674982583024 37.0 32.0 38.0 19.5 38.0 74-75 32.4100205686229 37.0 31.0 38.0 15.0 38.0 76 31.645174667418637 36.0 28.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.999633425784637 1101 2 -0.048351957492460684 1101 3 0.41801860158162185 1101 4 -2.7000612202271483 1101 5 -0.5002578805658686 1101 6 -1.6240143509746687 1101 7 -1.6090042936468407 1101 8 -1.194333448780366 1101 9 -0.9346210548246106 1101 10-11 -1.0388095701596214 1101 12-13 -0.9996785443503882 1101 14-15 0.8548235090167893 1101 16-17 -2.9250804115934095 1101 18-19 0.1840131101225353 1101 20-21 -0.740107791000824 1101 22-23 -0.18779085596242595 1101 24-25 -1.476178178446105 1101 26-27 -1.6233017848564586 1101 28-29 -0.044021249469743395 1101 30-31 -1.4463025207693363 1101 32-33 0.4479054128888862 1101 34-35 -1.3796327094112897 1101 36-37 -0.0012307552115800036 1101 38-39 -0.7087239508709189 1101 40-41 0.2594427571987765 1101 42-43 0.1441136757855901 1101 44-45 1.1551941271172197 1101 46-47 0.46878832126540004 1101 48-49 -1.9399256239565652 1101 50-51 -0.7734837887118786 1101 52-53 0.45443502281924 1101 54-55 -1.273803447297361 1101 56-57 -0.13676882382021915 1101 58-59 -0.17296231672622753 1101 60-61 0.00900692568367134 1101 62-63 -1.5183480384151125 1101 64-65 0.03859736981260653 1101 66-67 -0.6027284004981439 1101 68-69 0.665893078915829 1101 70-71 0.372912495941101 1101 72-73 -0.41797038399887043 1101 74-75 -1.5440717149745566 1101 76 -2.843840126941018 1102 1 0.3087408240789742 1102 2 -0.33203990075487155 1102 3 0.2576258028909528 1102 4 0.35749469903089803 1102 5 -0.07909039556859909 1102 6 0.8413429867230917 1102 7 1.4934234204830048 1102 8 1.8626769167406394 1102 9 1.7537313728895185 1102 10-11 0.02249703104059364 1102 12-13 -3.373314660007779 1102 14-15 -1.0565240087137084 1102 16-17 -2.199698523977478 1102 18-19 -1.1657550295392198 1102 20-21 0.09042957943016461 1102 22-23 -0.455998711936239 1102 24-25 0.5774224762183735 1102 26-27 -5.531716951250353 1102 28-29 0.045313174971063575 1102 30-31 0.41096698277675614 1102 32-33 -8.025430648213131 1102 34-35 -1.1146981758597363 1102 36-37 0.3597212360595563 1102 38-39 0.8763824321078033 1102 40-41 0.5328333736744995 1102 42-43 0.3230553015357245 1102 44-45 -6.443141933984801 1102 46-47 -0.3519972761159451 1102 48-49 0.32364502525238237 1102 50-51 -6.946287934920274 1102 52-53 -2.464891218315511 1102 54-55 0.9236188112951069 1102 56-57 -1.4288446557896677 1102 58-59 0.46854068381932024 1102 60-61 0.3725502972057697 1102 62-63 -4.429354585059958 1102 64-65 -0.8339476383706952 1102 66-67 0.06543853894539353 1102 68-69 -8.685238945088535 1102 70-71 -0.23333409434804508 1102 72-73 0.21146224011459935 1102 74-75 -5.922413231613945 1102 76 -0.8888483102470737 1103 1 0.2928879145134893 1103 2 -0.2621796213137557 1103 3 -0.17381899981133486 1103 4 0.08135467139386421 1103 5 -0.48857911044653335 1103 6 -2.272122390215337 1103 7 0.920914591986147 1103 8 1.3335044923586352 1103 9 0.7723479858175111 1103 10-11 0.2656453497877109 1103 12-13 -1.8098454445924546 1103 14-15 -0.8449471624063207 1103 16-17 -0.6220269440603872 1103 18-19 -0.49868532279885613 1103 20-21 -1.0015211345265413 1103 22-23 -0.978549763678906 1103 24-25 0.5451408855535504 1103 26-27 -0.7502606331173851 1103 28-29 0.36609468886635455 1103 30-31 0.358322272197924 1103 32-33 -4.942653894029192 1103 34-35 -0.8648325225509623 1103 36-37 -0.2159927206424186 1103 38-39 0.24065388919363784 1103 40-41 0.4901303182468908 1103 42-43 0.39752175324768757 1103 44-45 -4.0148062975653325 1103 46-47 0.14938457556531404 1103 48-49 0.31917319967276825 1103 50-51 -0.03292235838104318 1103 52-53 -0.23310479116980076 1103 54-55 0.12769143527791726 1103 56-57 0.7643650785877298 1103 58-59 1.1409842582090022 1103 60-61 0.7407561931062787 1103 62-63 0.39121581260624794 1103 64-65 0.7665244942870615 1103 66-67 -0.11846288896801838 1103 68-69 -2.5928659986736733 1103 70-71 0.7346146236006703 1103 72-73 1.4544762121704906 1103 74-75 -1.7965879590820855 1103 76 -0.36980946486092137 1104 1 -2.977957893869746 1104 2 0.2825434325784606 1104 3 0.2541001618653098 1104 4 0.5401870067232046 1104 5 0.319467296739095 1104 6 -0.38013137225126314 1104 7 0.6624939333035158 1104 8 1.7652410193047388 1104 9 -0.37158913993098963 1104 10-11 -0.0585125843440224 1104 12-13 -0.5875774805205936 1104 14-15 0.4195176579529587 1104 16-17 0.24284955294559296 1104 18-19 0.7610077909736006 1104 20-21 1.1714391948147806 1104 22-23 0.8999307752432486 1104 24-25 0.9802269633978611 1104 26-27 1.1145971513137525 1104 28-29 0.21109843138131623 1104 30-31 -0.6536163505565753 1104 32-33 -0.3320812892387721 1104 34-35 0.5861031061915511 1104 36-37 0.959961620674946 1104 38-39 0.4117990987744733 1104 40-41 1.2402051685462894 1104 42-43 1.6748982502536762 1104 44-45 -2.8550009083437757 1104 46-47 0.8107431084994445 1104 48-49 1.4449591278164817 1104 50-51 1.7199780907207511 1104 52-53 0.6267754483511538 1104 54-55 0.5658463753976761 1104 56-57 1.2621008570308376 1104 58-59 1.3476272222808632 1104 60-61 0.936732989513466 1104 62-63 1.5238505431451728 1104 64-65 0.33600428470622745 1104 66-67 0.4930827697146256 1104 68-69 -2.8817133040628917 1104 70-71 0.4377396236006703 1104 72-73 0.7562539067812679 1104 74-75 -0.4434068213575415 1104 76 0.6922414333426694 1105 1 -1.590242376628364 1105 2 -0.6079019697203911 1105 3 -0.8719055852611248 1105 4 -0.20837621166759845 1105 5 0.06149315880805872 1105 6 0.11455253579470792 1105 7 1.9861289907747803 1105 8 1.2813329733277286 1105 9 0.7725200554713041 1105 10-11 1.0924572432421868 1105 12-13 -2.103310239141287 1105 14-15 -0.878363089173483 1105 16-17 0.7809601851295085 1105 18-19 0.43493020476670097 1105 20-21 0.03483718332052632 1105 22-23 0.023996867197276117 1105 24-25 0.3835674806392433 1105 26-27 0.2274563467160462 1105 28-29 -1.0229892122968423 1105 30-31 -0.41518244251059855 1105 32-33 0.13957819351984568 1105 34-35 0.45327264642143206 1105 36-37 0.3923251264220653 1105 38-39 0.03442840911930034 1105 40-41 0.3291419501554884 1105 42-43 0.37522152611573745 1105 44-45 1.507786447978063 1105 46-47 0.725146844131622 1105 48-49 0.0914390128739555 1105 50-51 -0.22811817364706855 1105 52-53 -1.4864429424534364 1105 54-55 -1.2062441418437047 1105 56-57 -0.33412759124501434 1105 58-59 -0.7789173179490234 1105 60-61 -0.6894523553141241 1105 62-63 0.1772988190072411 1105 64-65 -0.2600086463282594 1105 66-67 0.4916100685652012 1105 68-69 1.169867155707223 1105 70-71 1.6915111753248127 1105 72-73 -0.17359523114976128 1105 74-75 -1.0581338328517873 1105 76 -1.1222700609102034 1106 1 -1.1065763149223748 1106 2 0.17234606415740927 1106 3 -0.5807682591873196 1106 4 0.13887121724951967 1106 5 0.034269928318042986 1106 6 0.16000020669610393 1106 7 1.5194018280403583 1106 8 1.0705041771994814 1106 9 1.8988055969111102 1106 10-11 0.39395452091913796 1106 12-13 0.10551462474256113 1106 14-15 0.5213268684792709 1106 16-17 0.7851192897877013 1106 18-19 0.3126525278157075 1106 20-21 -0.5836594893957425 1106 22-23 0.27460182787482523 1106 24-25 0.9659177528715475 1106 26-27 0.25834715131374963 1106 28-29 -1.2870923580923659 1106 30-31 -0.5177610873986822 1106 32-33 0.6624910791822813 1106 34-35 0.39432679040207574 1106 36-37 0.7443366206749431 1106 38-39 0.19436488824815967 1106 40-41 0.6073104317041924 1106 42-43 1.3605890397273583 1106 44-45 1.3588148811299092 1106 46-47 0.7826181084994417 1106 48-49 0.30120912781648457 1106 50-51 0.7865899328260113 1106 52-53 -0.1896719200698982 1106 54-55 0.1357476911871558 1106 56-57 1.0017390149255831 1106 58-59 -0.08181356719282462 1106 60-61 1.1109106210924082 1106 62-63 0.9192452799872726 1106 64-65 0.5562345478641149 1106 66-67 1.0725235591883049 1106 68-69 1.6399972222528945 1106 70-71 0.04711462360067031 1106 72-73 0.6116815383602159 1106 74-75 -2.2588673476733234 1106 76 -0.6518375140257504 1107 1 -0.5188570166767619 1107 2 0.4983694559702734 1107 3 0.2832668285319784 1107 4 0.1417951938577069 1107 5 0.5138020920607325 1107 6 0.47067272131598514 1107 7 0.21530826078889476 1107 8 1.5529603175503581 1107 9 -1.2342353387614011 1107 10-11 0.46778493027586165 1107 12-13 0.5291257358536683 1107 14-15 -0.7173427221639983 1107 16-17 0.5446222137643133 1107 18-19 1.349933229570091 1107 20-21 1.020872674346947 1107 22-23 -0.5251057744643504 1107 24-25 -0.5099594401109044 1107 26-27 -0.23397741008975714 1107 28-29 -0.7699139955192607 1107 30-31 -0.12777570728172094 1107 32-33 1.3532805528664866 1107 34-35 0.6476162640862881 1107 36-37 -0.05025402259990841 1107 38-39 0.9582537771370454 1107 40-41 0.11206189369249131 1107 42-43 0.032007168382328643 1107 44-45 -0.211360557466584 1107 46-47 0.023846178674880036 1107 48-49 0.5205073734305259 1107 50-51 0.7679495819488196 1107 52-53 1.019757904491506 1107 54-55 0.7267564631169776 1107 56-57 0.6548822897793869 1107 58-59 0.7138735673101024 1107 60-61 -0.5586800221824504 1107 62-63 -0.4670851293694511 1107 64-65 0.2302111560512543 1107 66-67 0.2245703428140402 1107 68-69 1.1655820175745362 1107 70-71 -0.03731520096073382 1107 72-73 0.18076048572864067 1107 74-75 -0.5501685172639696 1107 76 -0.6452585666573292 1108 1 -0.22890486356671502 1108 2 -1.0108277795427512 1108 3 -0.8318846866195386 1108 4 -0.259055417519221 1108 5 -0.2898130062912081 1108 6 0.7793383247184309 1108 7 0.7471530242126079 1108 8 1.11372586778959 1108 9 -1.2848467156885661 1108 10-11 -0.4345542510106881 1108 12-13 0.13874827705516424 1108 14-15 0.6252941731044714 1108 16-17 -2.1023209015998567 1108 18-19 -0.19296948175367135 1108 20-21 0.3338444978450781 1108 22-23 -0.7468495277870559 1108 24-25 -1.2944889456930468 1108 26-27 -1.195061939595341 1108 28-29 0.09111737077525817 1108 30-31 -0.14585119904142374 1108 32-33 0.35854371076123215 1108 34-35 -0.1364347725963313 1108 36-37 0.5284275297658496 1108 38-39 0.7339581896835625 1108 40-41 0.21179607763720298 1108 42-43 0.7475308260112499 1108 44-45 0.26886272801986166 1108 46-47 0.7326181084994445 1108 48-49 -0.1775787509713922 1108 50-51 -0.2827681214004585 1108 52-53 0.5510178725935759 1108 54-55 0.23999410267040133 1108 56-57 0.7498849479399325 1108 58-59 -1.6255735352948975 1108 60-61 1.159554959210432 1108 62-63 -0.39281612352149864 1108 64-65 -0.0722343516574071 1108 66-67 0.028025951532804072 1108 68-69 0.8292715444219567 1108 70-71 1.001281290267336 1108 72-73 0.14025011890248607 1108 74-75 0.7060249968242829 1108 76 0.36686264546388614 1109 1 -1.0371148706139302 1109 2 -1.4812647069564235 1109 3 -0.52293472185562 1109 4 -0.11388276071865633 1109 5 -0.7123640986097435 1109 6 0.32539188356268767 1109 7 -1.8600351364639245 1109 8 0.6338456704675295 1109 9 0.33974806937133195 1109 10-11 0.8664147412373779 1109 12-13 9.399613398670681E-4 1109 14-15 0.22351475097621432 1109 16-17 -1.0152173075195137 1109 18-19 -0.615591046235707 1109 20-21 1.2639537296984997 1109 22-23 0.5369947287316208 1109 24-25 0.7419275447932137 1109 26-27 0.4138704071277033 1109 28-29 0.2697466871952727 1109 30-31 0.2578662075829641 1109 32-33 0.8800553386682068 1109 34-35 -0.041949219389842085 1109 36-37 0.20945289974471137 1109 38-39 0.9820025871465674 1109 40-41 0.2753795871509439 1109 42-43 0.48398255257925626 1109 44-45 -0.43784974555308054 1109 46-47 -0.6011028217331145 1109 48-49 -0.7075118024160716 1109 50-51 0.5008647186277315 1109 52-53 0.7250312623046398 1109 54-55 0.2696981195837225 1109 56-57 0.2903712058680483 1109 58-59 -0.24009080097495428 1109 60-61 0.6840440360250923 1109 62-63 0.0796644966335407 1109 64-65 -0.038777692037960776 1109 66-67 0.29039381622624916 1109 68-69 1.3046239052394313 1109 70-71 0.5747309026704386 1109 72-73 -0.020126907172219433 1109 74-75 1.4151687600378153 1109 76 -1.5173515899131438 1110 1 -0.26036396323390676 1110 2 -0.06229038245044194 1110 3 -0.8631686242618564 1110 4 -0.48799218402823996 1110 5 -0.3347595818736231 1110 6 -0.6910417768755437 1110 7 0.4527757252110334 1110 8 1.887206337223816 1110 9 -1.300996654381862 1110 10-11 -1.092165040991425 1110 12-13 -0.6049546481506525 1110 14-15 -0.3233833536077384 1110 16-17 0.34760027548895067 1110 18-19 0.41270576785220925 1110 20-21 -0.29635343524302016 1110 22-23 0.3527125671507676 1110 24-25 0.042925662819826016 1110 26-27 -1.31860371573827 1110 28-29 0.3205095585489488 1110 30-31 0.09280705984805593 1110 32-33 1.5625899535357917 1110 34-35 0.2099831639950125 1110 36-37 -0.16101020013430656 1110 38-39 0.054609792416087544 1110 40-41 -0.6065433863670009 1110 42-43 0.23131082250800716 1110 44-45 1.1157360858758736 1110 46-47 0.4054504784416366 1110 48-49 0.8214403416893177 1110 50-51 0.5634571369635282 1110 52-53 -0.13854247072399062 1110 54-55 -0.9219607055271766 1110 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0.8755418882181871 2116 56-57 0.09679636985135431 2116 58-59 -0.6853054700268331 2116 60-61 -0.236424061768588 2116 62-63 1.1667992610938853 2116 64-65 1.5090010795780202 2116 66-67 0.9244128979197441 2116 68-69 0.7218123369627492 2116 70-71 -2.358334094348045 2116 72-73 0.6762058298581906 2116 74-75 -1.326259385460098 2116 76 -0.8696175410163036 2117 1 0.48636172638342146 2117 2 0.4063567237176997 2117 3 0.8760938327513834 2117 4 -0.41709147428945315 2117 5 -1.1779219437672346 2117 6 -1.0492769418715184 2117 7 -3.4526168261901553 2117 8 -1.5853918920876637 2117 9 0.23141718918293464 2117 10-11 -0.2242166982680729 2117 12-13 0.40902062074522405 2117 14-15 -0.5354269623002139 2117 16-17 -0.17499063692782357 2117 18-19 -1.4892295507985551 2117 20-21 -0.4634896026535813 2117 22-23 0.2859276106862936 2117 24-25 0.5171336089674838 2117 26-27 0.3667332272631185 2117 28-29 0.21311583644461507 2117 30-31 -0.3036954644806258 2117 32-33 1.097784217090343 2117 34-35 0.08665690365990741 2117 36-37 -1.6053469236288578 2117 38-39 0.26456808611624894 2117 40-41 -0.8985606542385156 2117 42-43 -1.1079735851893702 2117 44-45 0.34199276254229716 2117 46-47 -0.0306730307410632 2117 48-49 -1.3443604924366852 2117 50-51 0.4211252426194889 2117 52-53 1.1147501318954625 2117 54-55 -0.8113292575137123 2117 56-57 -1.5410241429691567 2117 58-59 -0.8490104359469868 2117 60-61 0.5253010274881476 2117 62-63 -0.8628583176143252 2117 64-65 -0.2035922342811176 2117 66-67 -1.248096027753732 2117 68-69 -0.21351710153124515 2117 70-71 0.7346146236006703 2117 72-73 1.1566099194395036 2117 74-75 0.11442545712347396 2117 76 -2.3667775539991 2118 1 0.6907921061302567 2118 2 1.226293432578462 2118 3 1.091600161865312 2118 4 0.7776870067232018 2118 5 0.9569672967390943 2118 6 0.43861862774873117 2118 7 1.712493933303513 2118 8 -3.2347589806952577 2118 9 -1.109089139930994 2118 10-11 -0.2835125843440167 2118 12-13 0.9842975194793979 2118 14-15 -0.008607342047049826 2118 16-17 -0.05090044705439567 2118 18-19 -0.2796172090264051 2118 20-21 -2.9316858051852215 2118 22-23 -0.4188192247567528 2118 24-25 0.4396019633978625 2118 26-27 1.0895971513137468 2118 28-29 -0.02327656861868377 2118 30-31 0.4932586494434261 2118 32-33 0.4397937107612293 2118 34-35 1.4454781061915511 2118 36-37 0.23496162067493742 2118 38-39 -0.1975759012255196 2118 40-41 -1.269169831453702 2118 42-43 -0.1438517497463252 2118 44-45 0.7199990916562271 2118 46-47 -0.1392568915005583 2118 48-49 -0.5269158721835154 2118 50-51 -0.09252190927924886 2118 52-53 -0.21697455164884616 2118 54-55 0.9439713753976733 2118 56-57 1.2152258570308376 2118 58-59 1.6632522222808603 2118 60-61 -1.3913920104865376 2118 62-63 0.6144755431451685 2118 64-65 -0.4327457152937768 2118 66-67 -2.21941723028538 2118 68-69 1.8307866959371069 2118 70-71 0.5033646236006675 2118 72-73 -0.0624960932187264 2118 74-75 1.3034681786424613 2118 76 0.8609914333426723 2119 1 -0.7513131570276386 2119 2 0.49471448521003936 2119 3 0.7087054250232079 2119 4 -0.15718141432942545 2119 5 0.7776909809496217 2119 6 0.9428949435382066 2119 7 -2.368756066696484 2119 8 0.7283989140415841 2119 9 -0.1867207188783624 2119 10-11 0.5288229419717752 2119 12-13 0.033146203689923937 2119 14-15 -0.7418310262575716 2119 16-17 -0.1556701838965111 2119 18-19 -0.6939264195527173 2119 20-21 -0.5902384367641673 2119 22-23 0.7219702489274553 2119 24-25 -0.07684540502319237 2119 26-27 0.5807155723663797 2119 28-29 0.9267234313813191 2119 30-31 0.1631599652329072 2119 32-33 -0.05790365765982841 2119 34-35 0.6739320535599731 2119 36-37 -0.765531800377687 2119 38-39 -1.0720824801728952 2119 40-41 -0.18874219987475982 2119 42-43 -1.2709899076410665 2119 44-45 -1.6707903820279846 2119 46-47 0.10169705586786648 2119 48-49 -0.659317187972988 2119 50-51 1.2043530907207511 2119 52-53 -1.5350666569120008 2119 54-55 -2.268857571970745 2119 56-57 1.304370593872953 2119 58-59 1.5793706433334904 2119 60-61 1.1832790421450383 2119 62-63 0.7876663326188549 2119 64-65 0.0332082320746494 2119 66-67 0.46397092760935976 2119 68-69 -0.20539751458920819 2119 70-71 -1.8377537974519633 2119 72-73 -3.079107935323993 2119 74-75 -0.43978840030490574 2119 76 0.5455309070268832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 5.0 13 49.0 14 145.0 15 316.0 16 453.0 17 530.0 18 509.0 19 510.0 20 479.0 21 491.0 22 486.0 23 535.0 24 597.0 25 714.0 26 891.0 27 989.0 28 1217.0 29 1506.0 30 1927.0 31 2384.0 32 3098.0 33 4052.0 34 5281.0 35 7937.0 36 12615.0 37 12567.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.078791221648366 22.584365441508297 11.905597619883334 31.431245716960003 2 17.16650631987526 20.192084397704278 36.09295690541751 26.548452377002953 3 18.244700262084066 25.050592177288262 28.920479049862323 27.784228510765352 4 14.197326079023323 18.208207544040075 37.56593570646585 30.028530670470754 5 12.518661048999768 35.472580698669674 34.83561689281093 17.173141359519622 6 26.875228079487773 39.16000398102379 17.412002786716652 16.552765152771787 7 26.092293401453077 30.776631390372554 22.983777328069536 20.147297880104833 8 22.00345022061507 35.11262979796304 21.05298079156023 21.83093918986166 9 23.222638755266562 17.5247984606708 22.43638655740968 36.81617622665295 10-11 24.933649603556383 26.00437912616528 26.429851043359985 22.632120226918357 12-13 23.537803138373754 24.130809806588594 26.390870185449355 25.940516869588297 14-15 21.435324951066583 25.517533092260226 24.083535149122515 28.963606807550672 16-17 22.09551139568059 27.74939455263245 26.08565836180871 24.069435689878247 18-19 21.574660783598183 26.979729953886473 26.628902232690837 24.816707029824503 20-21 21.14172444680357 27.42261885014763 26.625584712868662 24.81007199018014 22-23 22.470391135587032 26.843711641177055 26.840394121354876 23.845503101881032 24-25 21.59456590253127 27.849749527253426 26.67451813024583 23.88116643996948 26-27 21.090302889559766 28.0106492386292 27.070961748996453 23.828086122814586 28-29 21.72809607537405 26.80390140331089 28.699034601731743 22.768967919583318 30-31 21.859138108350198 27.206150681750323 27.71290183458846 23.221809375311018 32-33 20.921938758584083 28.34654812062502 27.355439073748467 23.37607404704243 34-35 21.551438144842916 27.56029592276814 27.44667086885844 23.441595063530503 36-37 22.452974156520586 26.981388713797568 26.917526457220582 23.648110672461268 38-39 21.543144345287462 27.467405367747073 27.833991308098067 23.1554589788674 40-41 21.698238396974425 26.275586371628574 28.17486646982716 23.85130876156985 42-43 21.076203430315495 26.103904720830705 28.257804465381682 24.562087383472118 44-45 20.503931260989283 25.68175032345818 28.55057558968915 25.263742825863382 46-47 20.744451448097404 25.505921772882594 29.44796470158909 24.301662077430912 48-49 19.818034037753378 25.738977540390806 30.05009454931493 24.39289387254089 50-51 19.367680721892313 26.536011677669773 29.437182762167 24.65912483827091 52-53 18.549912085724714 26.66207743091265 29.57900673456524 25.2090037487974 54-55 18.372424775238034 27.284941777527123 28.874863152307334 25.46777029492751 56-57 18.707494277278304 26.586603854958035 28.857446173240888 25.848455694522777 58-59 18.892446007364892 26.18186643665196 28.732209799953555 26.193477756029594 60-61 19.055833858607304 25.874995853100224 29.049032942971838 26.020137345320638 62-63 18.82360747105464 26.07736456225326 28.286003383870217 26.813024582821882 64-65 20.001327007928875 26.154496898118968 27.549513983346053 26.294662110606108 66-67 20.0311846863285 26.098099061141887 26.811365822910794 27.059350429618817 68-69 19.288060246159972 25.813621736389873 27.21693262117241 27.68138539627774 70-71 20.38284178747968 26.191818996118503 26.54679361709186 26.878545599309955 72-73 20.503931260989283 26.36101250704973 26.244899313273397 26.89015691868759 74-75 20.070165544239128 27.092525627840626 26.37842948611618 26.458879341804064 76 20.870517201340277 26.15864379789669 26.493713299936967 26.477125700826065 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.5 15 2.0 16 2.0 17 1.0 18 1.5 19 3.5 20 7.5 21 10.0 22 20.0 23 35.0 24 50.0 25 60.0 26 92.0 27 159.5 28 241.0 29 287.0 30 364.5 31 506.5 32 745.5 33 920.0 34 1015.5 35 1321.5 36 1653.5 37 1775.0 38 2014.0 39 2612.0 40 3190.5 41 3643.0 42 3876.0 43 3967.0 44 4313.5 45 4563.0 46 4557.0 47 4591.5 48 4475.5 49 4041.0 50 3757.0 51 3545.0 52 2934.5 53 2365.0 54 2194.0 55 2018.5 56 1572.0 57 1131.0 58 961.0 59 838.0 60 603.0 61 446.5 62 402.0 63 335.5 64 233.5 65 177.0 66 133.5 67 111.0 68 92.0 69 62.5 70 40.0 71 28.0 72 27.5 73 22.0 74 15.0 75 13.0 76 9.0 77 5.5 78 5.0 79 4.0 80 4.5 81 4.0 82 4.0 83 5.0 84 3.0 85 2.0 86 3.0 87 3.0 88 2.5 89 2.0 90 2.0 91 1.0 92 0.0 93 1.0 94 1.5 95 1.5 96 2.0 97 1.5 98 1.5 99 3.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7580532793683442 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 60286.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.99260193079654 #Duplication Level Percentage of deduplicated Percentage of total 1 75.57519717160729 46.095279169293036 2 11.093282567310307 13.532163354676044 3 5.39026380201251 9.862986431343927 4 2.725047593146587 6.648309723650598 5 1.6970356268697306 5.175330922602263 6 1.0905629589339134 3.9909763460836674 7 0.6336687516997552 2.7054374149885545 8 0.4868098993744901 2.3753441926815513 9 0.4079412564590699 2.239325879972133 >10 0.9001903725863476 7.374846564708225 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004976279733271406 0.0 2 0.0 0.0 0.0 0.008293799555452343 0.0 3 0.0 0.0 0.0 0.01327007928872375 0.0 4 0.0 0.0 0.0 0.019905118933085626 0.0 5 0.0 0.0 0.0 0.019905118933085626 0.0 6 0.0 0.0 0.0 0.021563878844176095 0.0 7 0.0 0.0 0.0 0.0265401585774475 0.0 8 0.0 0.0 0.0 0.0265401585774475 0.0 9 0.0 0.0 0.0 0.03483395813289984 0.0 10 0.0 0.0 0.0 0.04976279733271406 0.0 11 0.0 0.0 0.0 0.05473907706598547 0.0 12 0.0 0.0 0.0 0.06303287662143782 0.0 13 0.0 0.0 0.0 0.06635039644361874 0.0 14 0.0 0.0 0.0 0.06966791626579968 0.0 15 0.0 0.0 0.0 0.0796204757323425 0.0 16 0.0 0.0 0.0 0.0796204757323425 0.0 17 0.0 0.0 0.0 0.08791427528779484 0.0 18 0.0 0.0 0.0 0.08957303519888532 0.0 19 0.0 0.0 0.0 0.09786683475433766 0.0 20 0.0 0.0 0.0 0.1011843545765186 0.0 21 0.0 0.0 0.0 0.10616063430979 0.0 22 0.0 0.0 0.0 0.1111369140430614 0.0 23 0.0 0.0 0.0 0.1327007928872375 0.0 24 0.0 0.0 0.0 0.1376770726205089 0.0 25 0.0 0.0 0.0 0.14597087217596125 0.0 26 0.0 0.0 0.0 0.15260591182032313 0.0 27 0.0 0.0 0.0 0.17251103075340876 0.0 28 0.0 0.0 0.0 0.19241614968649437 0.0 29 0.0 0.0 0.0 0.23554390737484657 0.0 30 0.0 0.0 0.0 0.26374282586338454 0.0 31 0.0 0.0 0.0 0.3085293434628272 0.0 32 0.0 0.0 0.0 0.3599509007066317 0.0 33 0.0 0.0 0.0 0.39478485883953157 0.0 34 0.0 0.0 0.0 0.43791261652788377 0.0 35 0.0 0.0 0.0 0.5324619314600405 0.0 36 0.0 0.0 0.0 0.661845204525097 0.0 37 0.0 0.0 0.0 0.9388581096772053 0.0 38 0.0 0.0 0.0 1.3568656072720036 0.0 39 0.0 0.0 0.0 1.9490428955313008 0.0 40 0.0 0.0 0.0 2.657333377566931 0.0 41 0.0 0.0 0.0 3.584580167866503 0.0 42 0.0 0.0 0.0 4.709219387585841 0.0 43 0.0 0.0 0.0 5.760873171217198 0.0 44 0.0 0.0 0.0 6.699731280894404 0.0 45 0.0 0.0 0.0 7.7082573068374085 0.0 46 0.0 0.0 0.0 8.693560694025146 0.0 47 0.0 0.0 0.0 9.66227648210198 0.0 48 0.0 0.0 0.0 10.513220316491392 0.0 49 0.0 0.0 0.0 11.18335932057194 0.0 50 0.0 0.0 0.0 11.861792124207943 0.0 51 0.0 0.0 0.0 12.677902000464453 0.0 52 0.0 0.0 0.0 13.65988786783001 0.0 53 0.0 0.0 0.0 14.701589091994824 0.0 54 0.0 0.0 0.0 15.690209999004743 0.0 55 0.0 0.0 0.0 16.529542514016523 0.0016587599110904688 56 0.0 0.0 0.0 17.312477192051222 0.0016587599110904688 57 0.0 0.0 0.0 18.05560163221975 0.0016587599110904688 58 0.0 0.0 0.0 18.729058156122484 0.0016587599110904688 59 0.0 0.0 0.0 19.477158876024284 0.0016587599110904688 60 0.0 0.0 0.0 20.039478485883954 0.0016587599110904688 61 0.0 0.0 0.0 20.638290813787613 0.0016587599110904688 62 0.0 0.0 0.0 21.343263776001063 0.0016587599110904688 63 0.0 0.0 0.0 22.166008691901933 0.0016587599110904688 64 0.0 0.0 0.0 22.952260889758815 0.0016587599110904688 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACTTC 15 0.0022153873 69.99419 11 TACATGC 15 0.0022153873 69.99419 2 GTACATG 20 0.0067646746 52.80238 1 ACATAGA 20 0.0067646746 52.80238 1 TTAGGCG 20 0.0069225384 52.495644 3 TATTCCC 20 0.0069225384 52.495644 5 TTGCGCT 20 0.0069225384 52.495644 14 GTCAAAG 20 0.0069225384 52.495644 22 TTCGGTC 20 0.0069225384 52.495644 28 GATTGCG 20 0.0069225384 52.495644 12 TCGGTCA 20 0.0069225384 52.495644 29 TACCACT 40 0.0024023228 34.997097 40 AATCACT 55 2.9615941E-4 31.81554 27 GCTGTTA 45 0.004278183 31.108528 18 AGGTGGC 45 0.004278183 31.108528 8 AGATGTG 45 0.004278183 31.108528 5 GAATTGA 120 2.2118911E-9 29.164246 70 TTAGGGT 50 0.0071600145 27.997677 58 ACTTGTT 50 0.0071600145 27.997677 36 TCTTAGG 50 0.0071600145 27.997677 29 >>END_MODULE