FastQCFastQC Report
Wed 25 May 2016
SRR1780652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60286
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT7021.164449457585509No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2950.4893341737716883RNA PCR Primer, Index 30 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC940.15592343164250408No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC830.1376770726205089No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTGT150.002214657870.0000135
GTGTCTC150.002214657870.0000138
TGAGCGA150.002214657870.0000117
AGGTGTC150.002214657870.0000136
CGAGTGA252.8084323E-670.064
TCTCGAG252.8084323E-670.061
ACGTCTC252.8084323E-670.058
CTCGAGT252.8084323E-670.062
ATGATTC352.9285366E-760.00000440
AGACTTC308.2845145E-658.3333366
GCGAACT308.2845145E-658.33333623
GTCTCGA308.2845145E-658.33333660
GATCCAA308.2845145E-658.33333643
ACTCAAT308.2845145E-658.33333635
CAACGGA252.3728352E-455.99999659
AAGACTT252.3728352E-455.9999965
AACGGAA252.3728352E-455.99999660
ACGGAAC252.3728352E-455.99999661
ACCAACG252.3728352E-455.99999657
CCAACGG252.3728352E-455.99999658
Produced by FastQC (version 0.11.5)