##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780652_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60286 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.02871313406098 34.0 34.0 34.0 32.0 34.0 2 33.33606475798693 34.0 34.0 34.0 33.0 34.0 3 33.49304979597253 34.0 34.0 34.0 34.0 34.0 4 33.5142985104336 34.0 34.0 34.0 34.0 34.0 5 33.501526059118206 34.0 34.0 34.0 34.0 34.0 6 37.24995853100222 38.0 37.0 38.0 36.0 38.0 7 36.833560030521184 38.0 37.0 38.0 35.0 38.0 8 36.88951000232226 38.0 37.0 38.0 35.0 38.0 9 36.698586736555754 38.0 37.0 38.0 34.0 38.0 10-11 36.38016289022327 38.0 37.0 38.0 33.5 38.0 12-13 36.28541452410178 38.0 37.0 38.0 34.0 38.0 14-15 36.52250107819394 38.0 37.0 38.0 34.0 38.0 16-17 36.6597966360349 38.0 37.0 38.0 34.0 38.0 18-19 36.694837939156685 38.0 37.0 38.0 34.0 38.0 20-21 36.416879540855255 38.0 37.0 38.0 34.0 38.0 22-23 36.49618485220449 38.0 37.0 38.0 34.0 38.0 24-25 35.82041435822579 38.0 36.5 38.0 32.5 38.0 26-27 36.02843943867565 38.0 37.0 38.0 33.0 38.0 28-29 35.87876953189796 38.0 37.0 38.0 31.5 38.0 30-31 35.65477888730385 38.0 36.5 38.0 31.5 38.0 32-33 36.1136250539097 38.0 37.0 38.0 34.0 38.0 34-35 36.15911654447136 38.0 37.0 38.0 33.0 38.0 36-37 36.299522277145606 38.0 37.0 38.0 34.0 38.0 38-39 35.89194008559201 38.0 36.5 38.0 32.5 38.0 40-41 35.464701589091995 38.0 36.0 38.0 31.0 38.0 42-43 35.868261287861195 38.0 36.5 38.0 32.5 38.0 44-45 36.07476860299241 38.0 37.0 38.0 33.5 38.0 46-47 36.36962147098829 38.0 37.0 38.0 34.0 38.0 48-49 36.17899678200577 38.0 37.0 38.0 34.0 38.0 50-51 36.30828882327572 38.0 37.0 38.0 34.0 38.0 52-53 36.24290050758053 38.0 37.0 38.0 34.0 38.0 54-55 36.43341737716882 38.0 37.0 38.0 34.0 38.0 56-57 36.48998109013701 38.0 37.0 38.0 34.0 38.0 58-59 36.53007331718807 38.0 37.0 38.0 34.0 38.0 60-61 36.212022691835585 38.0 37.0 38.0 33.0 38.0 62-63 36.48358657067976 38.0 37.0 38.0 34.0 38.0 64-65 36.487343661878384 38.0 37.0 38.0 34.0 38.0 66-67 35.89360713930266 38.0 37.0 38.0 31.5 38.0 68-69 35.345544570878815 38.0 36.0 38.0 29.0 38.0 70-71 35.33750787910958 38.0 36.0 38.0 30.5 38.0 72-73 35.08112994725143 38.0 36.0 38.0 28.0 38.0 74-75 35.69521945393624 38.0 36.5 38.0 31.5 38.0 76 35.14119364363202 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46225151852544144 1101 2 0.5700563817786417 1101 3 0.04368759970545 1101 4 0.4556821946737344 1101 5 0.4436922522332125 1101 6 -1.1844286370326316 1101 7 0.12782246410212394 1101 8 0.516700588610334 1101 9 0.7877103040443032 1101 10-11 0.3640011009608557 1101 12-13 0.5563954905605968 1101 14-15 0.6514999311517542 1101 16-17 -0.3166474823630594 1101 18-19 -0.36456156445990473 1101 20-21 0.21821354018140937 1101 22-23 0.695218881984232 1101 24-25 -0.7164674016929027 1101 26-27 0.031232993488828242 1101 28-29 1.1377571841437728 1101 30-31 1.2360748886753186 1101 32-33 0.35184907798834075 1101 34-35 0.6250462853303915 1101 36-37 0.4229109007621332 1101 38-39 0.41686939764175435 1101 40-41 1.3336171103957497 1101 42-43 0.8674079982538174 1101 44-45 0.28952404002978227 1101 46-47 0.6050702848257572 1101 48-49 -1.3097464912103405 1101 50-51 -1.3940503073037505 1101 52-53 -1.3949315898095662 1101 54-55 -0.14965619478937953 1101 56-57 -0.9437825899100716 1101 58-59 -0.7658767062925662 1101 60-61 -0.7879567943601273 1101 62-63 -0.33140913116488235 1101 64-65 -0.7277596346889368 1101 66-67 -0.8722672034625987 1101 68-69 -0.2150436831611131 1101 70-71 -0.7336198986865199 1101 72-73 -1.4686839243416472 1101 74-75 -0.22536730087323775 1101 76 0.3215574741107048 1102 1 -0.9089977995323864 1102 2 0.042778694926482785 1102 3 0.2324491927223633 1102 4 0.32952289298251713 1102 5 0.10163005910718681 1102 6 0.3151621976645842 1102 7 -0.13567999088969174 1102 8 0.4599629999578525 1102 9 0.656368241851176 1102 10-11 0.5739029012881858 1102 12-13 0.6818728500805165 1102 14-15 0.01722551762202329 1102 16-17 0.2695500080897446 1102 18-19 -0.02447700363066474 1102 20-21 0.41277164165440894 1102 22-23 7.289747283749648E-4 1102 24-25 -5.4117074453372 1102 26-27 -1.7058782994735253 1102 28-29 0.002800828442737213 1102 30-31 1.436906858124317 1102 32-33 0.24123805780285323 1102 34-35 0.7001963126080781 1102 36-37 -2.577089099237874 1102 38-39 -0.2543662815726506 1102 40-41 1.0158320585681508 1102 42-43 0.8359022699395737 1102 44-45 0.9309588463582017 1102 46-47 0.9630217305431543 1102 48-49 -3.7213667858093658 1102 50-51 -1.008548397865674 1102 52-53 -0.21714926575064908 1102 54-55 0.29810703488875134 1102 56-57 0.6141824946507626 1102 58-59 -0.05065575702906244 1102 60-61 -5.43280131154507 1102 62-63 -2.477344755823907 1102 64-65 -0.21030191510355678 1102 66-67 0.9821381429640255 1102 68-69 -0.55956086810383 1102 70-71 -1.5888435757187054 1102 72-73 -5.725980705574603 1102 74-75 -2.1064365861978445 1102 76 -0.3846618384915885 1103 1 0.6659292694066536 1103 2 0.33826464610105944 1103 3 0.04912353634281175 1103 4 0.2793539999992518 1103 5 0.20480831797407006 1103 6 0.2715954849361992 1103 7 0.7794129002511028 1103 8 0.7553721815945735 1103 9 0.4149664300900824 1103 10-11 0.08630118165054768 1103 12-13 -0.44602519997322787 1103 14-15 -0.1373691392181442 1103 16-17 0.28659284549186737 1103 18-19 0.7757110817370574 1103 20-21 -0.2480029629824756 1103 22-23 0.539190513189908 1103 24-25 -3.124608566172455 1103 26-27 -0.41835718785061715 1103 28-29 -0.3867469989793477 1103 30-31 0.9654459338190833 1103 32-33 1.0182609351251344 1103 34-35 0.06489000215206175 1103 36-37 -0.43237852039555946 1103 38-39 0.22504028989979474 1103 40-41 0.337035804921598 1103 42-43 -0.38799608719231315 1103 44-45 1.0730208761447884 1103 46-47 1.0135936636001261 1103 48-49 -1.750078592964286 1103 50-51 -0.12095819365870142 1103 52-53 0.33184889178044585 1103 54-55 0.28332889885312795 1103 56-57 0.7947828324367237 1103 58-59 0.19777046744506777 1103 60-61 -4.245637178273153 1103 62-63 -1.0936506056626953 1103 64-65 0.44561701626447103 1103 66-67 -0.16489471439817294 1103 68-69 0.41894366129889704 1103 70-71 0.8363253172645599 1103 72-73 -4.567125339383907 1103 74-75 -1.497270303378869 1103 76 -3.0778139957156014 1104 1 0.4887586107240196 1104 2 0.35720177184956015 1104 3 0.3463915004146685 1104 4 -0.2230412095815879 1104 5 -0.1700045562774264 1104 6 0.08214937715176518 1104 7 0.6207302655205638 1104 8 0.6516296666245154 1104 9 1.0550861905691278 1104 10-11 -0.7259368423015502 1104 12-13 0.1603183629010303 1104 14-15 0.5383793637758671 1104 16-17 0.2777230850128305 1104 18-19 0.8087762014975368 1104 20-21 -0.047965537832773464 1104 22-23 0.19544051318990796 1104 24-25 0.7290778110730542 1104 26-27 0.8850672133469857 1104 28-29 1.4154610848529927 1104 30-31 0.6309773709448336 1104 32-33 0.3796194680592677 1104 34-35 0.06389823568499509 1104 36-37 0.962494234095459 1104 38-39 1.4256016671453011 1104 40-41 1.2724025713886604 1104 42-43 0.4824567571190599 1104 44-45 0.7323210258453869 1104 46-47 0.9069319869534169 1104 48-49 -0.05413922170679086 1104 50-51 0.5422528841856078 1104 52-53 0.16650458040319904 1104 54-55 0.09874806052977192 1104 56-57 -0.21073737714411322 1104 58-59 -0.5234121672854712 1104 60-61 0.3973268935831342 1104 62-63 0.2810687057145529 1104 64-65 0.5985923156656696 1104 66-67 -0.0770766006257162 1104 68-69 1.2748942601012914 1104 70-71 1.3637205268453911 1104 72-73 1.2314712175023175 1104 74-75 0.17505379841754376 1104 76 -0.37729004361979435 1105 1 0.8518333233676998 1105 2 0.2748741856426662 1105 3 0.0746242590353603 1105 4 -0.16097224406434663 1105 5 -0.3240993838636328 1105 6 0.2216608714046373 1105 7 0.6552130241412542 1105 8 -0.7469335517662898 1105 9 0.722902282523151 1105 10-11 0.4423821232156868 1105 12-13 -0.7880652577886238 1105 14-15 0.20677016837357343 1105 16-17 0.1913006712197216 1105 18-19 0.5470305118423653 1105 20-21 0.33529595642010435 1105 22-23 -0.0757520155457243 1105 24-25 0.2829212018776488 1105 26-27 0.13309163863434037 1105 28-29 0.44742947565758584 1105 30-31 1.0402446123241447 1105 32-33 0.7319542381742039 1105 34-35 -0.8623589482230472 1105 36-37 0.6183131996127003 1105 38-39 -0.28122304549837907 1105 40-41 -0.5201980033239835 1105 42-43 0.0012426766592952276 1105 44-45 0.7311356810177969 1105 46-47 0.502477963964914 1105 48-49 -0.4704826125113897 1105 50-51 0.6140201255649202 1105 52-53 0.29136090224228184 1105 54-55 0.4501848421389738 1105 56-57 0.31135314009726756 1105 58-59 0.1856395568524576 1105 60-61 0.6803728705946313 1105 62-63 -1.0704039954348659 1105 64-65 0.2589730627921085 1105 66-67 0.6279162154662359 1105 68-69 -0.38825229162284103 1105 70-71 0.9864216762706874 1105 72-73 0.4370387462379526 1105 74-75 -0.6212464889387803 1105 76 0.5194053586790588 1106 1 -1.1171624419075563 1106 2 0.07364914027061786 1106 3 0.19145728988835486 1106 4 0.14143247462894237 1106 5 -0.07954402996163878 1106 6 0.040702008730711725 1106 7 -0.8463749976373336 1106 8 0.34373492978241416 1106 9 -1.4541243357466627 1106 10-11 -0.10718684230155162 1106 12-13 1.0367986260589177 1106 14-15 -0.5642522151714999 1106 16-17 -0.2752374413029699 1106 18-19 0.2168354120238547 1106 20-21 0.20038972532511679 1106 22-23 0.05892735529516813 1106 24-25 -0.8164813994532594 1106 26-27 0.6260211607154105 1106 28-29 0.35460582169510246 1106 30-31 0.709595791997458 1106 32-33 -0.0129792161512583 1106 34-35 0.4522206041060528 1106 36-37 0.7990073919902017 1106 38-39 0.34566745661898324 1106 40-41 0.5450999398097167 1106 42-43 0.5895291255401176 1106 44-45 0.663077604792754 1106 46-47 0.6536425132692116 1106 48-49 0.9776041993458335 1106 50-51 -0.1936023789722796 1106 52-53 0.44479405408741 1106 54-55 -0.2815150973649665 1106 56-57 0.17988762285588678 1106 58-59 0.4568509906092686 1106 60-61 0.4828532093726068 1106 62-63 0.5795884425566626 1106 64-65 -1.1703221580185428 1106 66-67 0.024732609900596003 1106 68-69 0.789038996943404 1106 70-71 -0.10798999947039789 1106 72-73 0.17406990171284775 1106 74-75 -0.29418962263508774 1106 76 -0.33321109625137524 1107 1 0.521726446981333 1107 2 -0.011877175518854699 1107 3 -0.18500469841573874 1107 4 0.12608159743595593 1107 5 0.02425713962900744 1107 6 0.3294446987891959 1107 7 0.5008472245848878 1107 8 0.09738990054264463 1107 9 -0.09520620709169236 1107 10-11 0.3493190056516582 1107 12-13 0.06603839214078988 1107 14-15 -0.14027560698436758 1107 16-17 0.45173624290756464 1107 18-19 0.48986172781333437 1107 20-21 0.5761207194771671 1107 22-23 -0.3058387265761766 1107 24-25 -0.2101217503304511 1107 26-27 -0.2961279620916102 1107 28-29 0.3447374006424653 1107 30-31 -0.09340128402592995 1107 32-33 0.670500316012479 1107 34-35 0.19198668597739754 1107 36-37 0.7679401405282107 1107 38-39 0.2915738893675197 1107 40-41 0.019880641564100188 1107 42-43 0.4210349734933274 1107 44-45 0.129451873798601 1107 46-47 0.892677600988506 1107 48-49 0.9129112168896967 1107 50-51 0.7625379719049121 1107 52-53 0.22768879092951266 1107 54-55 0.2988942593601891 1107 56-57 0.6239665702243116 1107 58-59 0.3987369555215423 1107 60-61 1.012092975454479 1107 62-63 0.7736673899250732 1107 64-65 0.5467831051393546 1107 66-67 0.02875307773685165 1107 68-69 0.7528547864170818 1107 70-71 -0.5454899994703908 1107 72-73 1.058938322765485 1107 74-75 -0.6838095056760238 1107 76 0.74646726632173 1108 1 -1.022983813518401 1108 2 0.09848965063743975 1108 3 -0.023494863221692697 1108 4 0.2527163661759886 1108 5 0.2195787770559079 1108 6 -0.47959304709065975 1108 7 -0.3338151890248895 1108 8 -1.3764006364057835 1108 9 -0.4873380518551187 1108 10-11 -0.21362623624094823 1108 12-13 0.4977236659313249 1108 14-15 -0.014650939254430284 1108 16-17 -0.7807996422599004 1108 18-19 0.026863322709658632 1108 20-21 0.5870723409551033 1108 22-23 -0.28202160802221243 1108 24-25 0.14394523531547776 1108 26-27 -0.9953305139257367 1108 28-29 -2.190031339389435 1108 30-31 -0.3116362654188123 1108 32-33 0.11967628624108073 1108 34-35 0.25509141750318065 1108 36-37 0.7713957492469774 1108 38-39 0.6360183338119612 1108 40-41 0.1377434804795712 1108 42-43 -1.2844940004566965 1108 44-45 0.5119611773605399 1108 46-47 0.5398865324079622 1108 48-49 0.6609175964750236 1108 50-51 0.5181998538825852 1108 52-53 -0.26114693474831796 1108 54-55 0.20102078780249855 1108 56-57 0.18329671376497458 1108 58-59 -0.0306091369824415 1108 60-61 0.6117208329770776 1108 62-63 0.5660118875327314 1108 64-65 0.04470973990809313 1108 66-67 0.37415445998034613 1108 68-69 1.135310926767957 1108 70-71 -0.8703703822455111 1108 72-73 -0.8506310552249516 1108 74-75 -1.1400030197642792 1108 76 0.3162705624408133 1109 1 -0.8417646450899312 1109 2 0.2417947951053776 1109 3 0.1040949887867626 1109 4 0.07579599972073936 1109 5 -0.16491734697510196 1109 6 -0.32511806470869686 1109 7 -0.4315953158747874 1109 8 0.051048271275682566 1109 9 0.296946655685403 1109 10-11 0.2431038553728726 1109 12-13 0.4127166187149811 1109 14-15 0.6715188986595919 1109 16-17 0.7418219222221296 1109 18-19 0.4887907363812616 1109 20-21 0.6838658575160608 1109 22-23 0.539190513189908 1109 24-25 -0.2221576540432224 1109 26-27 0.02787244590512472 1109 28-29 -1.4646261244493317 1109 30-31 -0.1403889081249332 1109 32-33 0.2677735378267059 1109 34-35 -0.40579653175686303 1109 36-37 0.5760116759559253 1109 38-39 0.43272376016855674 1109 40-41 0.3019083853421449 1109 42-43 0.7794771059562677 1109 44-45 0.27032974677561583 1109 46-47 0.06536221951155596 1109 48-49 0.30494508061877923 1109 50-51 -0.02882269720974051 1109 52-53 -0.16750704750378276 1109 54-55 -0.6268333348190609 1109 56-57 -0.4058681910976034 1109 58-59 -0.21381914402965663 1109 60-61 0.2752338703273196 1109 62-63 -0.03906210823893019 1109 64-65 0.5873277807819477 1109 66-67 -0.2643585773699044 1109 68-69 -0.5563557398987058 1109 70-71 -0.07058179873600068 1109 72-73 -0.4279037824976797 1109 74-75 -0.47182120158245766 1109 76 0.3578843749848559 1110 1 -1.0507645106632602 1110 2 -0.36295718768801066 1110 3 -0.0841359562327284 1110 4 -0.11263658530413068 1110 5 0.3186153859191023 1110 6 -0.04147779047828948 1110 7 0.3114817106072678 1110 8 0.7335660828094888 1110 9 0.9620226067540969 1110 10-11 -0.5352937787177368 1110 12-13 0.4779303571206839 1110 14-15 0.9652579764926301 1110 16-17 0.14994129310530724 1110 18-19 -0.0787418620862752 1110 20-21 0.752793132687458 1110 22-23 -0.7064742266944819 1110 24-25 -0.8496613507766568 1110 26-27 0.8365664908036337 1110 28-29 0.365424957685363 1110 30-31 -0.019203264893320693 1110 32-33 0.3697025605448161 1110 34-35 0.7489957790375996 1110 36-37 1.062602615598351 1110 38-39 0.7918950197464554 1110 40-41 -1.4973589893049848 1110 42-43 0.11354418486472895 1110 44-45 0.8546656790245706 1110 46-47 0.5337383453349176 1110 48-49 1.325394737830777 1110 50-51 -0.5711502950051397 1110 52-53 0.27423579427603073 1110 54-55 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2116 38-39 -3.507571409777775 2116 40-41 -0.644424351688258 2116 42-43 -0.35320029416298837 2116 44-45 0.1873691027684643 2116 46-47 -0.14274750022607208 2116 48-49 0.03824859880602105 2116 50-51 -0.7104714747887471 2116 52-53 0.5296456060442196 2116 54-55 0.02887626565797774 2116 56-57 -0.14863801816975553 2116 58-59 -2.2365531929264932 2116 60-61 -0.843057721801479 2116 62-63 0.7277834493042903 2116 64-65 -1.3288916586933084 2116 66-67 -0.44414390831802564 2116 68-69 -0.5563557398987058 2116 70-71 1.5329512960761704 2116 72-73 0.9278654482715467 2116 74-75 0.38715315739190004 2116 76 -0.16030286413261052 2117 1 0.4949294967999691 2117 2 0.4045118984318421 2117 3 0.3186225130728957 2117 4 -0.09899057667018951 2117 5 0.1381441778997896 2117 6 0.17502912398720838 2117 7 0.6523758351408162 2117 8 0.3671359957384439 2117 9 -0.3917492524688484 2117 10-11 -0.22031975369395695 2117 12-13 -1.033154738364793 2117 14-15 0.1212907561809331 2117 16-17 -1.2990965352403379 2117 18-19 -0.9886525959708194 2117 20-21 -0.04677882897201613 2117 22-23 -0.062075309594902706 2117 24-25 -2.1980187079142866 2117 26-27 -1.9414755081720045 2117 28-29 -1.001864864514097 2117 30-31 -0.2765384518399827 2117 32-33 -1.6744548990293495 2117 34-35 0.34479222302676504 2117 36-37 -0.826034246917196 2117 38-39 -0.06039516829774527 2117 40-41 -0.1738790741809595 2117 42-43 0.3452336558532423 2117 44-45 -0.6110729614963901 2117 46-47 -0.8580996586162044 2117 48-49 -1.0022009305675539 2117 50-51 0.015591491780547528 2117 52-53 0.4438779981247194 2117 54-55 -0.42625193947022666 2117 56-57 0.11896990133689656 2117 58-59 -0.846988116652561 2117 60-61 -1.1612174102143342 2117 62-63 -0.5727683196018987 2117 64-65 -0.9637098995242042 2117 66-67 -0.4782237525244568 2117 68-69 -0.08958358799996802 2117 70-71 -0.48153263771155963 2117 72-73 0.521463306109915 2117 74-75 0.4174193047466588 2117 76 0.8089441335953964 2118 1 0.07625861072402529 2118 2 0.23845177184956157 2118 3 -0.3973584995853301 2118 4 0.23945879041841778 2118 5 0.4174954437225722 2118 6 0.4008993771517666 2118 7 0.8082302655205638 2118 8 0.7516296666245168 2118 9 -1.563663809430878 2118 10-11 -0.34781184230155304 2118 12-13 -0.2303066370989697 2118 14-15 0.9290043637758671 2118 16-17 -2.4410269149871766 2118 18-19 0.049401201497545344 2118 20-21 -0.4135905378327678 2118 22-23 0.5766905131899023 2118 24-25 1.210327811073057 2118 26-27 -1.5961827866530172 2118 28-29 -0.012663915147008709 2118 30-31 -0.43152262905517347 2118 32-33 0.3202444680592649 2118 34-35 -0.3111017643150049 2118 36-37 -2.8312557659045368 2118 38-39 0.35372666714529544 2118 40-41 0.6599025713886633 2118 42-43 1.0230817571190656 2118 44-45 -1.189553974154613 2118 46-47 0.2444319869534155 2118 48-49 0.13336077829320203 2118 50-51 -0.548372115814395 2118 52-53 -0.9428704195968081 2118 54-55 -0.6356269394702281 2118 56-57 0.6611376228558896 2118 58-59 -0.46716216728546556 2118 60-61 -0.5339231064168715 2118 62-63 -0.8126812942854471 2118 64-65 0.2485923156656753 2118 66-67 0.5604233993742795 2118 68-69 -0.39698073989870863 2118 70-71 0.376220526845394 2118 72-73 1.2408462175023232 2118 74-75 0.4156787984175452 2118 76 0.6977099563802085 2119 1 0.7052059791450773 2119 2 -0.7618771755188547 2119 3 0.11908886883572478 2119 4 -0.0625148937921125 2119 5 0.22308754898573113 2119 6 -0.48561378074297323 2119 7 -1.7082171029004911 2119 8 -0.4983703333754832 2119 9 -0.15807170416771754 2119 10-11 -0.41310789493312683 2119 12-13 -0.7428066370989725 2119 14-15 -0.23201537306623266 2119 16-17 -0.6041848097240177 2119 18-19 -0.9344803774498303 2119 20-21 -1.6055313273064584 2119 22-23 0.23984840792675044 2119 24-25 1.0782554426520008 2119 26-27 0.3036527396627733 2119 28-29 -0.5171047046206922 2119 30-31 0.5813063183132527 2119 32-33 0.6810997312171523 2119 34-35 0.7482732356849979 2119 36-37 -0.039808397483483304 2119 38-39 -1.397753596012592 2119 40-41 -0.48779479703239303 2119 42-43 -0.032181400775677105 2119 44-45 0.19597234163485666 2119 46-47 -0.16214696041500787 2119 48-49 -0.384237905917324 2119 50-51 0.8919239368171858 2119 52-53 0.3987414225084578 2119 54-55 -0.02164667631233641 2119 56-57 -1.1556386929335858 2119 58-59 -0.5168332199170464 2119 60-61 0.11114268305681918 2119 62-63 -0.10133261007491967 2119 64-65 -0.025585315913275508 2119 66-67 0.4753905046374385 2119 68-69 0.4535126811539243 2119 70-71 -0.38101631525986335 2119 72-73 1.3220962175023203 2119 74-75 -0.044189622635087744 2119 76 0.1536310090117894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 3.0 23 9.0 24 32.0 25 45.0 26 93.0 27 145.0 28 287.0 29 516.0 30 834.0 31 1290.0 32 2164.0 33 3217.0 34 5044.0 35 7812.0 36 14003.0 37 24792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.44627276647978 23.053445244335336 12.63145672295392 27.868825266230967 2 17.564608698536972 20.006303287662146 38.80337060013934 23.625717413661544 3 18.203231264306805 24.916232624489933 29.977109113226952 26.903426997976315 4 13.819128819294695 18.712470557011578 38.09010383837043 29.378296785323293 5 12.969843744816375 36.42636764754669 37.40503599509007 13.19875261254686 6 28.190624688982517 39.30929237302193 17.778588727067643 14.72149421092791 7 26.505324619314603 29.607205653053775 23.935905517035465 19.95156421059616 8 22.623826427362904 37.44152871313406 20.887104800451183 19.047540059051855 9 23.81813356334804 16.51958995454998 22.880934213581927 36.781342268520056 10-11 24.98424178084464 26.353548087449823 27.330557675082108 21.33165245662343 12-13 24.57203994293866 23.584248415884286 26.639684172112926 25.204027469064126 14-15 21.76790631324022 25.146800252131506 23.16624091828949 29.919052516338784 16-17 22.70095876322861 28.333278041336296 25.593836048170388 23.371927147264703 18-19 22.378329960521516 27.47321102743589 26.90093885810968 23.247520153932918 20-21 21.46352386955512 27.75354145241018 26.686958829579005 24.095975848455694 22-23 22.742427761005874 27.9061473642305 26.702717048734364 22.64870782602926 24-25 22.698470623361974 28.47593139369008 26.41658096407126 22.409017018876686 26-27 21.828451049995024 28.81514779550808 27.113260126729255 22.243141027767642 28-29 22.1286865939024 27.37368543277046 28.505789072089705 21.991838901237433 30-31 21.509139767110106 26.730915967222906 28.748797399064458 23.011146866602527 32-33 22.75984474007232 27.056032909796635 26.779020004644526 23.405102345486515 34-35 22.878446073715292 26.93328467637594 27.697143615433102 22.49112563447567 36-37 23.225956275088745 27.26006037886076 26.760773645622532 22.75320970042796 38-39 21.436154331022127 27.208638821616958 28.18316026938261 23.172046577978303 40-41 21.511627906976745 25.70331420230236 29.117871479282087 23.66718641143881 42-43 22.49776067412003 25.878313372922403 29.148558537637264 22.475367415320306 44-45 20.73615764854195 26.139568058919156 29.379126165278834 23.74514812726006 46-47 21.917194705238362 24.543011644494577 29.25637793185814 24.283415718408918 48-49 20.87881100089573 25.053909697110438 30.02604253060412 24.04123677138971 50-51 20.062701124639222 25.744783200079617 30.171184022824537 24.021331652456624 52-53 19.147895033672828 26.045848123942537 31.535514049696445 23.270742792688186 54-55 18.610456822479517 26.238264273629035 30.405898550243837 24.745380353647615 56-57 19.38924460073649 26.024284245098368 29.14607039777063 25.44040075639452 58-59 18.396476793948842 26.0707295226089 30.2474869787347 25.285306704707562 60-61 19.706897123710316 26.86195800019905 28.704010881465013 24.727133994625618 62-63 18.24221212221743 28.253657565603955 27.92024682347477 25.583883488703847 64-65 19.04256377931858 28.806024615997078 26.07155890256444 26.079852702119894 66-67 18.66934279932323 28.665859403509934 25.56149022990412 27.103307567262714 68-69 18.533324486613807 28.944531068573138 25.578907208970573 26.94323723584248 70-71 19.093155956606843 28.367282619513652 25.458647115416515 27.080914308462994 72-73 19.40500281989185 28.108516073383537 25.064691636532526 27.42178947019208 74-75 19.48130577580201 28.222970507248778 25.298576783996285 26.997146932952926 76 20.13402780081611 27.263377898682943 25.246325846796935 27.356268453704015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.5 15 0.5 16 3.0 17 6.0 18 6.5 19 8.0 20 10.5 21 12.0 22 19.0 23 27.0 24 47.0 25 66.0 26 104.5 27 161.0 28 240.5 29 302.0 30 372.0 31 501.5 32 771.0 33 981.0 34 1079.5 35 1376.0 36 1696.5 37 1819.0 38 2083.0 39 2639.5 40 3218.5 41 3815.5 42 4126.0 43 4323.5 44 4729.0 45 4920.0 46 4903.0 47 4842.5 48 4430.0 49 3865.5 50 3653.0 51 3434.5 52 2821.0 53 2228.0 54 2030.0 55 1897.0 56 1440.0 57 971.5 58 827.0 59 686.0 60 463.5 61 322.5 62 263.0 63 240.0 64 162.0 65 107.5 66 93.5 67 79.0 68 61.5 69 33.5 70 17.0 71 11.0 72 8.5 73 3.5 74 1.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 60286.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.94244103108516 #Duplication Level Percentage of deduplicated Percentage of total 1 59.769690706429465 26.86195800019905 2 16.98531040082675 15.267226221676674 3 8.798996087694693 11.863450884119032 4 4.727984055510445 8.499485784427561 5 2.9379198346497377 6.601864446140065 6 1.7014837233335793 4.588129914076236 7 1.321325754779656 4.156852337192714 8 0.8968775374621688 3.224629267159871 9 0.7197165424079133 2.911123643963773 >10 2.118550232523806 13.90040805493813 >50 0.014763416254521298 0.47108781474969313 >100 0.0036908540636303245 0.4893341737716883 >500 0.0036908540636303245 1.164449457585509 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 702 1.164449457585509 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 295 0.4893341737716883 RNA PCR Primer, Index 30 (95% over 22bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 94 0.15592343164250408 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 83 0.1376770726205089 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004976279733271406 0.0 2 0.0 0.0 0.0 0.008293799555452343 0.0 3 0.0 0.0 0.0 0.01327007928872375 0.0 4 0.0 0.0 0.0 0.019905118933085626 0.0 5 0.0 0.0 0.0 0.019905118933085626 0.0 6 0.0 0.0 0.0 0.021563878844176095 0.0 7 0.0 0.0 0.0 0.0265401585774475 0.0 8 0.0 0.0 0.0 0.0265401585774475 0.0 9 0.0 0.0 0.0 0.03649271804399031 0.0 10 0.0 0.0 0.0 0.053080317154895 0.0 11 0.0 0.0 0.0 0.058056596888166404 0.0 12 0.0 0.0 0.0 0.06635039644361874 0.0 13 0.0 0.0 0.0 0.07132667617689016 0.0 14 0.0 0.0 0.0 0.0746441959990711 0.0 15 0.0 0.0 0.0 0.08625551537670438 0.0 16 0.0 0.0 0.0 0.08625551537670438 0.0 17 0.0 0.0 0.0 0.09454931493215672 0.0 18 0.0 0.0 0.0 0.09786683475433766 0.0 19 0.0 0.0 0.0 0.10616063430979 0.0 20 0.0 0.0 0.0 0.1111369140430614 0.0 21 0.0 0.0 0.0 0.11611319377633281 0.0 22 0.0 0.0 0.0 0.12108947350960422 0.0 23 0.0 0.0 0.0 0.1376770726205089 0.0 24 0.0 0.0 0.0 0.14099459244268986 0.0 25 0.0 0.0 0.0 0.1492883919981422 0.0 26 0.0 0.0 0.0 0.15592343164250405 0.0 27 0.0 0.0 0.0 0.1808048303088611 0.0 28 0.0 0.0 0.0 0.19905118933085625 0.0 29 0.0 0.0 0.0 0.24052018710811798 0.0 30 0.0 0.0 0.0 0.26871910559665596 0.0 31 0.0 0.0 0.0 0.3135056231960986 0.0 32 0.0 0.0 0.0 0.36326842052881264 0.0 33 0.0 0.0 0.0 0.41137245795043625 0.0 34 0.0 0.0 0.0 0.4511826958166075 0.0 35 0.0 0.0 0.0 0.5490495305709452 0.0 36 0.0 0.0 0.0 0.6999966824801778 0.0 37 0.0 0.0 0.0 1.0135023056762764 0.0 38 0.0 0.0 0.0 1.4746375609594267 0.0 39 0.0 0.0 0.0 2.0966725276183524 0.0 40 0.0 0.0 0.0 2.876289685830873 0.0 41 0.0 0.0 0.0 3.864910592840792 0.0 42 0.0 0.0 0.0 5.0393126098928445 0.0 43 0.0 0.0 0.0 6.210397107122715 0.0 44 0.0 0.0 0.0 7.233851972265534 0.0 45 0.0 0.0 0.0 8.340244832962878 0.0 46 0.0 0.0 0.0 9.386922336860962 0.0 47 0.0 0.0 0.0 10.443552400225592 0.0 48 0.0 0.0 0.0 11.395680589191521 0.0 49 0.0 0.0 0.0 12.132169989715688 0.0 50 0.0 0.0 0.0 12.857048070862223 0.0 51 0.0 0.0 0.0 13.757754702584348 0.0 52 0.0 0.0 0.0 14.840924924526425 0.0 53 0.0 0.0 0.0 15.987128023089937 0.0 54 0.0 0.0 0.0 17.048734366187837 0.0 55 0.0 0.0 0.0 17.954417277643234 0.0 56 0.0 0.0 0.0 18.811996151677008 0.0 57 0.0 0.0 0.0 19.619812228378066 0.0 58 0.0 0.0 0.0 20.331420230235874 0.0 59 0.0 0.0 0.0 21.145871346581295 0.0 60 0.0 0.0 0.0 21.75629499386259 0.0 61 0.0 0.0 0.0 22.381647480343695 0.0 62 0.0 0.0 0.0 23.124771920512224 0.0 63 0.0 0.0 0.0 24.01884351258999 0.0 64 0.0 0.0 0.0 24.848223468135224 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGGTGT 15 0.0022146578 70.00001 35 GTGTCTC 15 0.0022146578 70.00001 38 TGAGCGA 15 0.0022146578 70.00001 17 AGGTGTC 15 0.0022146578 70.00001 36 CGAGTGA 25 2.8084323E-6 70.0 64 TCTCGAG 25 2.8084323E-6 70.0 61 ACGTCTC 25 2.8084323E-6 70.0 58 CTCGAGT 25 2.8084323E-6 70.0 62 ATGATTC 35 2.9285366E-7 60.000004 40 AGACTTC 30 8.2845145E-6 58.333336 6 GCGAACT 30 8.2845145E-6 58.333336 23 GTCTCGA 30 8.2845145E-6 58.333336 60 GATCCAA 30 8.2845145E-6 58.333336 43 ACTCAAT 30 8.2845145E-6 58.333336 35 CAACGGA 25 2.3728352E-4 55.999996 59 AAGACTT 25 2.3728352E-4 55.999996 5 AACGGAA 25 2.3728352E-4 55.999996 60 ACGGAAC 25 2.3728352E-4 55.999996 61 ACCAACG 25 2.3728352E-4 55.999996 57 CCAACGG 25 2.3728352E-4 55.999996 58 >>END_MODULE