FastQCFastQC Report
Wed 25 May 2016
SRR1780651_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780651_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGGATGGGGTGTG11.282051282051282No Hit
GTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACCATACT11.282051282051282No Hit
CCGGTGGGGGGTGCTGGAGGCGCTGCTCAACAAACAGGGCGGGTCGGGGG11.282051282051282No Hit
GCCTAGGTGGGCCTTGTTGGCAAGGTGGCTTTTGAGCAAAGACCTGCAGG11.282051282051282No Hit
TCCTCAAGCATCGCCGGTGCCACGTTCTTGCCCTGTCTCTTATACACATC11.282051282051282No Hit
GGGTGAAGGGCCTCACCCTGGCCGAGCAGGAGGCCCTGCGCTGAGCGCCC11.282051282051282No Hit
CCCCTGCCGTCCCTCAGGGAGGACAGGGGTTGGCGTCCAGGCTCCCTCCA11.282051282051282No Hit
GTCTCTGCTAGTGTGGAGATAAATCATATTATGGCCAAGGGTCATGATCT11.282051282051282No Hit
CGCCAGGACCGCGTGATGACGCGCATGACCTGGGCGCTGTCTCTTATACA11.282051282051282No Hit
ATGGATCACAGGCCTCTCACCCTATTAAGCACCCACGGGAGCTCTCCATG11.282051282051282No Hit
GCTAGATATCCAGTACATCTTTTCTTTCTTGCGAAAACTGTGTGATACCT11.282051282051282No Hit
CCTAGGCGACCCAGACAATTATACCCTAGCCAACCCCTTAAACACCCCTC11.282051282051282No Hit
GCTTCATTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGAT11.282051282051282No Hit
CGATCGAGGTAAGTCTTTGAGGCGGGACGTTGCTGAGGTGGTGGGGGGGA11.282051282051282No Hit
CCCTACCCGGGGGCCTCCCCGCGGGCCACGCTCGGCGGGGGGCAACCCCC11.282051282051282No Hit
CAACTATAGAAATCATTCTAAGTCTCCTTTGCCATCTTCTAAAACCAGGT11.282051282051282No Hit
TTGTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAG11.282051282051282No Hit
ACATTGTTTGTTGGTGTATATATTGTAATTGAGATTGCTCGGGGGAATAG11.282051282051282No Hit
GATTGTTAGCGGTGTGGTCGGGTGTGTTATTATTCTGAATTTTGGGGGAG11.282051282051282No Hit
GGGTGAGGCCGGGCTCATCGAGGCTCCCGGCCACGCTCGCCGCGATCTGT11.282051282051282No Hit
GAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAATTAAGAATGGTTA11.282051282051282No Hit
CCATATATCTTGTGGCCTTGGGTGTGGCCAAGGCTGAGTCTGAGTAATTG11.282051282051282No Hit
TCGCGGCGCTGCCGGGGGCGGTCGCCGCGCGCCTGGGCGCGTGGGACTGT11.282051282051282No Hit
AGGGCGGGAGCGATGTGAGGTGCGTGGGGTCGGGCTCTTACTGTATACCG11.282051282051282No Hit
GCTACACCTTGACCTACCGTCTTTACGTGGGGAATTACGCTTCCTTTGCA11.282051282051282No Hit
CGACCCAGCAGTCCCTGCGCACCCTGGAATGGCTCTCGCGCAGAGAGAAC11.282051282051282No Hit
ATACACATCACACACTCGACTAGTCCGGGTATTGTTAATTAGGATTTTTA11.282051282051282No Hit
CTCGTAGTGCCCGAAGAGGACGTTCATCAGGGTGGACTTGCCGGCGCCGT11.282051282051282No Hit
GGTCCGTCCCTCACAAGCTAGGAGGCGTCCTTGCCCTATTACTCTCCCTC11.282051282051282No Hit
CTTCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATTAACCTACT11.282051282051282No Hit
TCGCATCACTGCTCTCCAGTCTGGGTTACAGGCTAAGACCTGTCTCTTAT11.282051282051282No Hit
CCTTGGGGATGTACGAAGGACGTGCGCCGACGAGGGCCGGGACGGGGAGG11.282051282051282No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC11.282051282051282No Hit
GCGGTCAACTGGTCCTCCGTGAGGGCCGACAGGTCCGCCGGGCGGGCGAT11.282051282051282No Hit
AGGGCATCTGACGGAAACTAAGGCGGGAAGAGGCTCCCAGAGTGGCAATC11.282051282051282No Hit
GCCGGAGGACATCGCCCGGCAGCCTGGCGCCTTCCGCCCCCCGGCCCTTA11.282051282051282No Hit
CTATCACCCAGATTTAGTGTGAAAAGTAGTGTAATTGTAATTCACGCCAT11.282051282051282No Hit
CCGTCATGGCGCTCGAGCGGGTCCCGGCCGACATCCGCGCCCAGGGCGGC11.282051282051282No Hit
AGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGCTGTCTCTT11.282051282051282No Hit
GTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAACTTCTGAGGTAATAA11.282051282051282No Hit
ACACGAGGGTCGCGTGGGCGGGGGGGGGTGGAAGGGGGGGGGAGGGGGGT11.282051282051282No Hit
CTCCACGAGGCCGGCGTCCCGGCCACGCTCACGATGGTCTGTCTCTTATA11.282051282051282No Hit
GGTACGCCCCGCGCAGCAGCGCGAGGGCGAGCACGACGACCTGTCTCTTA11.282051282051282No Hit
GCACAAACACTGTCTCTTATACACATCTGACGCTGCCGACGATAAACTGG11.282051282051282No Hit
TGCCTGTTGAAACTTGATTGATGACCAGCCTCTGGCAGCCAGCTTATCCC11.282051282051282No Hit
CTCGGTGCGTCTGCACCTCGGCGACGACGCGCCGGAAGTCGTCCCGTGCG11.282051282051282No Hit
AATCCCTTCTCGTCCCCATGGATGACCCCCCTCAGATAGGGGTCCCTTGA11.282051282051282No Hit
CGAGGGAGACGATGGCCGCGTACTGCGAGTCGGCCGCGGAGGCGGTCGCC11.282051282051282No Hit
GGGCGTGATCATGAAAGGTGATAAGCTCTTCTATGATAGGGGAAGTAGCG11.282051282051282No Hit
GTCCACAGGACCGTGCCGGCGCACTTCTTCCGGCCCAAGAGACGATTTTC11.282051282051282No Hit
ACCGGGTCGAGGTCCCGCGGCCCGGACAGGCGCACCCCTGTCTCTTATAC11.282051282051282No Hit
CTATACACATCTTACGCTGCCGACGAACTTCTAGGTGGAGATCTCGGTGG11.282051282051282No Hit
GTGCAGAACCCGCCGAAGCTCTGGCCCAGTGTGGTCCCTGTCTCTTATAC11.282051282051282No Hit
ACTTCGCCGGCGCCGCTCAGGCGTAGCACGCGGTTGCGGGTCTCGCTCTT11.282051282051282No Hit
GTCCGAGTAACGTCGGGGCATTTCGGATAGGCCGGGAAAGTGTTGGGGGA11.282051282051282No Hit
GAGTGCGAGCGCGGCGGCGGCGGCCTCGGGCGCTGTCTCTTATACACATC11.282051282051282No Hit
GCCCTTATCAGAACTGGGAGGGAGATGGCGCTGTCTCTTATACACATCTG11.282051282051282No Hit
GGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCG11.282051282051282No Hit
GGCTAAGGTTGTCTGGTAGTAAGGTGGAGTGGGTTTGGGGCTAGGTTTAG11.282051282051282No Hit
GGCCTACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAATTTTT11.282051282051282No Hit
GGACGGCCGCTCGGCGCATCCACGAGATCTCCGGCGCGGCCGTCCTGGCC11.282051282051282No Hit
CATGCCCCCATGTCTAACAACATGGCTTTCTCAACTTTTAAAGGATAACA11.282051282051282No Hit
CTGCAAATCTGATGGTAACTTCTCCATCTGCGTGAGGGAGAGATTTCCAC11.282051282051282No Hit
GTGGTAATGTCCGCCAGGTTGGCCATGGCGAAGGGCGCGGATCCTCTCTC11.282051282051282No Hit
AGCCCGGAGACCATCAGGAGCACCCCGATCAGGATCGCCCCGCCGCCGGC11.282051282051282No Hit
GACATAGTGGAAGTGAGCTACAACGTAGTACGTGTAGTGTCGTCCGATGA11.282051282051282No Hit
ATTCAAGCAAGGCGGAAGGGGTTCCGCTGCAAATCAAGAAGGAGACCTCT11.282051282051282No Hit
GGATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAATGGTTGAT11.282051282051282No Hit
CTCCGGGGGATCCTGCCGTGGTCACGGCGTGGGTCTGCTCCTCCCCGCTC11.282051282051282No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA11.282051282051282No Hit
CCTTTGAGAGGCTGAGGTGGGGGGATTGCTTGAAGCCAAGAGTTCAAGAC11.282051282051282No Hit
CTCATGGTCACCGGCGTGTGGACGGACTGGACCTGTCTCTTATACACATC11.282051282051282No Hit
ACTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACT11.282051282051282No Hit
CAGCAGCACCATGGATATCACCTGGAAGTTTGTTAGAAATGCAGATTCTC11.282051282051282No Hit
GCCGCACAACTGGAGGTGAGTCGGCCACTGATCACCACGAGGCGTCAGGA11.282051282051282No Hit
CACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCTCCCCATGC11.282051282051282No Hit
GATCAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCGCACTGCGA11.282051282051282No Hit
GCCTTGTAGGTGTTGTTGCCGGTGTTCAGGTCCGGGAACACGAACACGGT11.282051282051282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGT50.044.010
GGAGAGA50.044.037
AATCTGA50.044.06
AAATCTG50.044.05
TGCGTGA50.044.029
TGGTAAC50.044.013
TGCAAAT50.044.02
TTTCCAC50.044.044
CATCTGC50.044.025
CTCCATC50.044.022
ATGGTAA50.044.012
CTGATGG50.044.09
ACTTCTC50.044.018
AGGGAGA50.044.035
GTGAGGG50.044.032
GCGTGAG50.044.030
GGGAGAG50.044.036
AACTTCT50.044.017
CTGCGTG50.044.028
TAACTTC50.044.016
Produced by FastQC (version 0.11.5)