FastQCFastQC Report
Wed 25 May 2016
SRR1780651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG33.8461538461538463Illumina PCR Primer Index 3 (96% over 28bp)
GCCTCGAAGATGCCCGGCTCCGAGGTGGCCGGCCAGGCCACCGTGTTCGT11.282051282051282No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT11.282051282051282TruSeq Adapter, Index 2 (95% over 21bp)
CTCCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATC11.282051282051282No Hit
GGGTGCGCCTGTCCGGGCCGCGGGACCTCGACCCGGTCTGTCTCTTATAC11.282051282051282No Hit
ACAGAACAGCTGATCGTCTATCAGCGATAGACCCTGATGGCGTGAATTAC11.282051282051282No Hit
TCTGACTGCTCTTATACACATCTCCGAGCCCACGAGACATGTTGATCTCG11.282051282051282No Hit
TCTCCGAGCCCACGAGACCGGTTCCCATATCGGATGCCGTCTTCTGCTTG11.282051282051282Illumina DpnII expression Adapter 2 (95% over 21bp)
GCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGC11.282051282051282No Hit
ATTCTCCTCCCTCAGCCTTCTGAGGAGCTGAGATTACAACCATGCACCAT11.282051282051282No Hit
CTCGAGGCGACCGCCTCCGCGGCCGACTCGCAGTACGCGGCCATCGTCTC11.282051282051282No Hit
GGTGCACACCCTCCTCGGCGAGAACGGCGCCGGCAAGTCCACCCTGATGA11.282051282051282No Hit
GTCCGGGCCGGGCAGGTGCATCCCGCCCTTGGCCAGGACGGCCGCGCCGG11.282051282051282No Hit
CTCTTATACACATCTCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG11.282051282051282No Hit
ACCTTGGGGCGCACACGCACGAGAAAATCGTCTCTTGGGCCGGAAGAAGT11.282051282051282No Hit
GCCCGGCGCACGCCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC11.282051282051282No Hit
CTGCTCGGCATCGCCGGCGGCGGGGCGATCCTGATCGGGGTGCTCCTGAT11.282051282051282No Hit
ATACACATCTCCGAGCCCAGAGACCGGTTCCCATCTCGTATGCCGTCTTC11.282051282051282RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACAGACCGGTTCCCATCTCGTATGCCGTC11.282051282051282No Hit
TGTTTGTGCCTGTCTCTTATACACATCTCCGAGCCCACGAACCCAACACG11.282051282051282No Hit
GTCCAGTGCGTCCACACGCCGGTGACCATGAGCTGTCTCTTATACACATC11.282051282051282No Hit
GGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATC11.282051282051282No Hit
CTTATACACATCTCCGAGACCACGAGACGATCCAAAATCTCGTATGCCGT11.282051282051282No Hit
CATGCGGGCCACGCCGCCCTGGGCGCGGATGTCGGCCGGGACCCGCTCGA11.282051282051282No Hit
CAACTACGCAAAGGCCCCAACGTTGTAGGCCCCCACCGGCTACTACAACC11.282051282051282No Hit
GGACCACACTGGGCCAGAGCTTCGGCGGGTTCTGCACCTGTCTCTTATAC11.282051282051282No Hit
TGCTTGTAAGCATGGGGAGGGGGTTTTGATGTGGATTGGGTTTTTATGTA11.282051282051282No Hit
TTTTCGCTTCCCACAGGTCTCCGAGCCCACGAGACTAACAACAATCTCGT11.282051282051282No Hit
GTCGTCGTGCTCGCCCTCGCGCTGCTGCGCGGGGCGTACCCTGTCTCTTA11.282051282051282No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT11.282051282051282No Hit
AATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAA11.282051282051282No Hit
CCCTGGCCCAGCTGTTAGCCCTCACGTTTGGGCCCCTTGTGCAGGTCTCT11.282051282051282No Hit
ACCTATTCCCCCGAGCAATCTCAATTACAATATATACACCAACAAACAAT11.282051282051282No Hit
TCCGTGCCGACCTCGTCCGAGCCCACGAGACGATCCAAAATCTCGTATGC11.282051282051282No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCCCGTAAATGATATCATCTC11.282051282051282No Hit
GATCCGCTCCCTGCGCCATGGCCAACATGGCGGACATTACCACCTGTCTC11.282051282051282No Hit
AGTATATCCAAAGACAACCATCATTCCCCCTAAATAAATTAAAAAAACTA11.282051282051282No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT11.282051282051282No Hit
TCTCCAGCCCACCACCTCCTCACTGCTCCAACAAGCCTCCCCACCGCCAG11.282051282051282No Hit
GCAGGACCCCGCCCAGCCTGGCCCCCGGCCAGCCCCCCCGGGAGGGCTGT11.282051282051282No Hit
CATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGGCC11.282051282051282No Hit
GTTATAGTAGTGTGCATGGTTATTACTTTTATTTGGAGTTGCACCAAAAT11.282051282051282No Hit
ACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCC11.282051282051282No Hit
AAAGTAGAATGGGGGTTGCCAGGGGCTGGGGAAATGAGGAGTTCAGGTCT11.282051282051282No Hit
GAGGTAGGTGGTAGTTTGTGTTTAATATTTTTAGTTGGGTGATGAGGAAT11.282051282051282No Hit
CCCTTAGAGGTCACGCTTTACAGTTTGACAAACGGCATTACGCAGGGCCA11.282051282051282No Hit
CTCCTGATGCGAGTAATACGGATGTGTTTAGGAGTGGGACTCTGTCTCTT11.282051282051282No Hit
ACGCGGGAGCGCGCGCCCCGCGTGAGCGGCGTCGGCGGCGTCTGTCTCTT11.282051282051282No Hit
GTCCAGCGCGATATCGGCGAAGGGATTGTTCTGCATGGACCTGTCTCTTA11.282051282051282No Hit
ATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTT11.282051282051282Illumina PCR Primer Index 6 (96% over 28bp)
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC11.282051282051282No Hit
GCCCCGCTGCTGCCGCAGATCGCCCGCCCGGCGGACCTGTCGGCCCTCAC11.282051282051282No Hit
CCTCAGATAGGGGTCCCTTGACCACCATCCTCCGTGAAATCAATATCCCG11.282051282051282No Hit
GGCCGCCGAAGGAGCAAACCCTCCCCGGGAATCTCTCAGGCATCCGTACC11.282051282051282No Hit
ACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCA11.282051282051282No Hit
CGCCCAGGTCATGCGCGTCATCACGCGGTCCTGGCGCTGTCTCTTATACA11.282051282051282No Hit
CCCGCAGACCACGAGGTTCTCTCTGCGCGAGAGCCATTCCAGGGTGCGCA11.282051282051282No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTAATGCATCTCGTATGCCGT11.282051282051282No Hit
AGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA11.282051282051282Illumina PCR Primer Index 6 (96% over 29bp)
CTACAAAACACTCATGAAAAAAGGAGAGCAGAGATGAAACAATCGCAAAT11.282051282051282No Hit
ACAAAGCAATCTGTGCGATTTCCATATTGCTGGCCTAAGAGATCTCCTTC11.282051282051282No Hit
CGCCTCGCCAACCCCACCGTGGTGGTCATCACCGACCGCCTGTCTCTTAT11.282051282051282No Hit
GGCAAGAACGTGGCACCGGCGATGCTTGAGGACTGTCTCTTATACACATC11.282051282051282No Hit
ACCATCGTGAGCGTGGCCGGGACGCCGGCCTCGTGGAGCTGTCTCTTATA11.282051282051282No Hit
CTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTACA11.282051282051282No Hit
GATGTGGGGAGGGGTGTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGT11.282051282051282No Hit
ATCATGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGACCT11.282051282051282No Hit
TAATGTTCTCAATTTAGTGGTGTCCATTACTATCATCATTATTGATTTCC11.282051282051282No Hit
TCACCCTCCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCA11.282051282051282No Hit
TCCCACGCGCCCAGGCGCGCGGCGACCGCCCCCGGCAGCGCCGCGACTGT11.282051282051282No Hit
CGCCCGAGGCCGCCGCCGCCGCGCTCGCACTCCTGTCTCTTATACACATC11.282051282051282No Hit
GGGATGCGGTGTCCGGAAGCTGCGTCGTCGTCACGGGCGTCACTCTACGG11.282051282051282No Hit
CTTATACACATCTCCGAGCCCACGAGATGGTCACAATCTCGTATGCCGTC11.282051282051282No Hit
GGTCATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGATAAGGGT11.282051282051282No Hit
CGCCATCTCCCTCCCAGTTCTGATAAGGGCCTGTCTCTTATACACATCTC11.282051282051282No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA11.282051282051282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAT50.044.032
AGCCCAC50.044.019
CATGATG50.044.030
GTATGCC50.044.044
ACGCCTC50.044.010
GCCTCCG50.044.012
ATCTCGT50.044.039
GCCCACG50.044.020
ACATGAT50.044.029
GATATCT50.044.036
CGCCTCC50.044.011
ATGATGA50.044.031
ATGATAT50.044.034
GGCGCAC50.044.05
CCCGGCG50.044.02
CACGCCT50.044.09
CCACGAG50.044.022
GAGCCCA50.044.018
CACGAGA50.044.023
CCCACGA50.044.021