FastQCFastQC Report
Wed 25 May 2016
SRR1780650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21453
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT250.11653381811401668No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC207.752867E-570.000013
TAAGACT150.002195176770.09
CTCTATA150.002195176770.02
TTCACCA200.006859855352.50000415
TGACTTC200.006859855352.50000422
CCAGTCA200.006859855352.50000419
GTACATG200.006859855352.5000041
GCGAACT200.006859855352.50000428
CTGACTT200.006859855352.50000421
TACATGC200.006859855352.5000042
GAACTAC200.006859855352.50000430
GTAAGTA200.006859855352.50000416
AGACTTC200.006859855352.50000411
AAAGCGA200.006859855352.50000425
GACTTCC200.006859855352.50000423
AGCGAAC200.006859855352.50000427
ACCAGTC200.006859855352.50000418
ACTATAA200.006859855352.5000043
ATCCTTC200.006859855352.50000450
TACTATA400.002367067635.0000042
Produced by FastQC (version 0.11.5)