##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780650_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21453 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.212464457185476 34.0 27.0 34.0 12.0 34.0 2 30.211718640749545 34.0 31.0 34.0 21.0 34.0 3 30.2583787815224 34.0 32.0 34.0 11.0 34.0 4 30.75374073556146 34.0 32.0 34.0 21.0 34.0 5 30.590267095511116 34.0 32.0 34.0 21.0 34.0 6 33.42343728149909 38.0 34.0 38.0 21.0 38.0 7 32.38540064326668 37.0 31.0 38.0 11.0 38.0 8 31.450333286719808 37.0 27.0 38.0 11.0 38.0 9 32.2684006898802 37.0 31.0 38.0 12.0 38.0 10-11 32.600312310632546 37.0 32.0 38.0 11.0 38.0 12-13 33.04707966251806 37.0 34.0 38.0 11.0 38.0 14-15 32.583321679951524 37.0 31.5 38.0 11.0 38.0 16-17 32.47415279914231 37.0 31.0 38.0 11.5 38.0 18-19 32.50619959912366 37.0 32.0 38.0 11.0 38.0 20-21 32.543840022374496 37.0 31.0 38.0 11.0 38.0 22-23 32.451149023446604 37.0 31.5 38.0 11.0 38.0 24-25 32.88290681955904 37.0 33.5 38.0 11.0 38.0 26-27 32.928052020696406 37.0 33.5 38.0 11.5 38.0 28-29 33.01142031417517 37.0 34.0 38.0 11.0 38.0 30-31 32.84172376823754 37.0 34.0 38.0 11.0 38.0 32-33 32.66123619074256 37.0 32.5 38.0 11.0 38.0 34-35 32.55020276884352 37.0 31.5 38.0 11.0 38.0 36-37 32.5050109541789 37.0 31.5 38.0 11.0 38.0 38-39 32.9100591991796 37.0 34.0 38.0 11.0 38.0 40-41 32.48352211811868 37.0 31.5 38.0 11.0 38.0 42-43 32.74980189250921 37.0 33.0 38.0 11.0 38.0 44-45 31.89929147438587 37.0 29.0 38.0 11.0 38.0 46-47 32.53092807532746 37.0 32.0 38.0 11.0 38.0 48-49 32.34037197594742 37.0 31.0 38.0 11.0 38.0 50-51 32.3514893021955 37.0 31.0 38.0 11.0 38.0 52-53 32.37062415512982 37.0 31.0 38.0 11.0 38.0 54-55 32.05383862396867 37.0 31.0 38.0 11.0 38.0 56-57 32.10942525520906 37.0 31.0 38.0 11.0 38.0 58-59 32.01260895911994 37.0 29.0 38.0 11.0 38.0 60-61 32.071947979303594 37.0 31.0 38.0 11.0 38.0 62-63 32.07362606628443 37.0 31.0 38.0 11.0 38.0 64-65 32.0864680930406 37.0 31.0 38.0 11.0 38.0 66-67 32.0143103528644 37.0 31.0 38.0 11.0 38.0 68-69 31.676805108842586 37.0 28.5 38.0 11.0 38.0 70-71 31.559339020183657 37.0 27.5 38.0 11.0 38.0 72-73 31.619097562112525 37.0 28.5 38.0 11.0 38.0 74-75 31.242040740222812 36.5 27.0 38.0 11.0 38.0 76 30.622104134619867 36.0 25.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.9610862894020684 1101 2 0.2728137842803484 1101 3 -1.38743879134012 1101 4 -2.416864150430772 1101 5 -1.347097653561363 1101 6 0.15388146849238638 1101 7 -0.5238234863082134 1101 8 1.7597133485813856 1101 9 1.6054883510777351 1101 10-11 0.1360962024947341 1101 12-13 -0.7160747266286549 1101 14-15 1.094423626242012 1101 16-17 -0.19395874103765465 1101 18-19 -0.03706089350647801 1101 20-21 -0.34192626035003215 1101 22-23 -0.021224806785376416 1101 24-25 -0.5453180870978187 1101 26-27 -0.7914243924053963 1101 28-29 0.5529439614428426 1101 30-31 -0.277670796241523 1101 32-33 1.111205206738994 1101 34-35 -1.0927753708893846 1101 36-37 0.2302891261620772 1101 38-39 0.10121998881964345 1101 40-41 0.9884405236241278 1101 42-43 -0.061710370344997045 1101 44-45 0.663118853872902 1101 46-47 -0.2131943831220795 1101 48-49 -0.21579470805569656 1101 50-51 -1.100428820376802 1101 52-53 0.8741898991246586 1101 54-55 -0.24764759540598646 1101 56-57 -0.16091241727272987 1101 58-59 0.6090348685997427 1101 60-61 1.1800839250927915 1101 62-63 -0.18044118869735115 1101 64-65 -0.6568166962788027 1101 66-67 -0.7291945057194624 1101 68-69 1.2143410552414302 1101 70-71 0.7750881627158854 1101 72-73 1.0167849798270154 1101 74-75 0.3241816638336914 1101 76 -1.1915014040168295 1102 1 -0.09071591903169818 1102 2 -0.08972182825526431 1102 3 -0.5277521816535113 1102 4 0.31461733105070877 1102 5 -0.5586361150998229 1102 6 0.32553389014481127 1102 7 1.6421309296461999 1102 8 2.243331582199616 1102 9 1.3277105732999566 1102 10-11 0.8843155757141119 1102 12-13 -1.6911459516998768 1102 14-15 0.18131821313659913 1102 16-17 -1.8983746954536045 1102 18-19 -0.20906944051502308 1102 20-21 0.8542988108750436 1102 22-23 -0.5002134107739806 1102 24-25 0.3841690923893566 1102 26-27 -5.0018944778754815 1102 28-29 -1.2013295428306598 1102 30-31 -0.16620355977428147 1102 32-33 -6.460019864486071 1102 34-35 0.08065767754366249 1102 36-37 0.5240925449654981 1102 38-39 0.6240120116116614 1102 40-41 0.328184113367719 1102 42-43 0.27304746441283356 1102 44-45 -6.913448097694051 1102 46-47 -0.5105590554867518 1102 48-49 0.42986056259957195 1102 50-51 -5.338320558268542 1102 52-53 -2.0079326079978514 1102 54-55 -0.7832601310185225 1102 56-57 -1.964331220691534 1102 58-59 0.3455021050669771 1102 60-61 -0.172836302827438 1102 62-63 -3.649814408070572 1102 64-65 -1.3053209697830788 1102 66-67 -0.42933695586191334 1102 68-69 -6.293849827949455 1102 70-71 -1.6066782190504973 1102 72-73 -0.06049422245218494 1102 74-75 -5.309009219357193 1102 76 -3.245632458147881 1103 1 -0.5235862894020684 1103 2 -0.4843977541811917 1103 3 -1.1759003298016601 1103 4 -0.4637391504307722 1103 5 0.17573888490017708 1103 6 -1.0865031468922268 1103 7 -0.40242925553898345 1103 8 -0.13571934372630778 1103 9 0.8711133510777351 1103 10-11 1.0333317794178143 1103 12-13 -1.925810303551728 1103 14-15 -1.6141100276041414 1103 16-17 -0.6194395102684211 1103 18-19 -1.6656666627372445 1103 20-21 -1.3719743372731088 1103 22-23 -0.883604614477683 1103 24-25 0.480523259056028 1103 26-27 -1.513179200097703 1103 28-29 0.22662184605822944 1103 30-31 0.15381958837386378 1103 32-33 -6.444684216337926 1103 34-35 -0.761645563197078 1103 36-37 -0.7901435661456162 1103 38-39 -0.16620789579574335 1103 40-41 -0.03379505329895238 1103 42-43 0.46112616811654306 1103 44-45 -5.841688838434791 1103 46-47 -1.7186030369682328 1103 48-49 -0.97060240036339 1103 50-51 -0.9141307434537254 1103 52-53 -0.9743677931830348 1103 54-55 -1.4513735569444464 1103 56-57 0.34990489041957673 1103 58-59 0.7592752532151295 1103 60-61 -0.3565747287533618 1103 62-63 -0.5488306117742745 1103 64-65 0.21638041910581052 1103 66-67 0.3513343404343843 1103 68-69 -1.5927502909124165 1103 70-71 0.05333335502357883 1103 72-73 0.09430901828855554 1103 74-75 -2.7329096823201553 1103 76 -0.41866486555528937 1104 1 -2.7154722543143492 1104 2 -0.20863021032154094 1104 3 -0.5969529613806088 1104 4 0.10506567413063195 1104 5 0.0027673936721086534 1104 6 -0.8255382346115283 1104 7 0.9569457444610165 1104 8 -0.2001382033754311 1104 9 -0.3506519997994566 1104 10-11 0.18451050748799247 1104 12-13 -0.42019078600786486 1104 14-15 0.05461256011515303 1104 16-17 -0.26143512430351024 1104 18-19 -0.10673025922847046 1104 20-21 0.09211557500758971 1104 22-23 1.1031004293819677 1104 24-25 -0.40969055673344457 1104 26-27 -0.2855202088696345 1104 28-29 -1.277352934643524 1104 30-31 -1.8294589203980642 1104 32-33 -0.5618717163379259 1104 34-35 -0.2785041158286532 1104 36-37 -0.7931589170228079 1104 38-39 -0.6430445186027569 1104 40-41 -0.16304395680771933 1104 42-43 -0.4355295336378404 1104 44-45 -3.820855505101459 1104 46-47 0.16146823496158902 1104 48-49 0.4625116347243292 1104 50-51 1.3405293442655761 1104 52-53 -0.17557393353391149 1104 54-55 -0.11708079378654901 1104 56-57 0.08537638164764516 1104 58-59 -0.11161290467960683 1104 60-61 -0.30490257963055356 1104 62-63 -0.1517637258093636 1104 64-65 0.46953283138650903 1104 66-67 0.1695909193817542 1104 68-69 -3.3138303347720672 1104 70-71 -0.02821818006414034 1104 72-73 0.544281606007857 1104 74-75 -1.855305515653491 1104 76 0.5750303098833065 1105 1 -0.40936215147103283 1105 2 0.7429729354739791 1105 3 -0.8138313642844182 1105 4 0.040032401293366604 1105 5 1.217225953865693 1105 6 0.42642788759053474 1105 7 1.1983250548058422 1105 8 1.3346470355840339 1105 9 1.6572124890087672 1105 10-11 2.1225020380384976 1105 12-13 -0.034377113896553624 1105 14-15 1.6905774723958586 1105 16-17 1.5712932483522692 1105 18-19 0.7583635096765491 1105 20-21 0.6371851454855104 1105 22-23 1.4771173682809362 1105 24-25 1.024973690090512 1105 26-27 1.448297093005742 1105 28-29 0.4548007253685782 1105 30-31 0.162979071132483 1105 32-33 0.11916276642069334 1105 34-35 0.53819279887189 1105 36-37 1.8267745373026614 1105 38-39 1.3890183973077015 1105 40-41 1.773586412218286 1105 42-43 1.6664063405303366 1105 44-45 2.0871904719100343 1105 46-47 1.232097394066244 1105 48-49 0.8720700134297203 1105 50-51 0.6402873599945522 1105 52-53 -0.8310488276657964 1105 54-55 0.24771049477969598 1105 56-57 0.4312626490402671 1105 58-59 -0.15290147092279938 1105 60-61 0.297789495384567 1105 62-63 0.7154474054671063 1105 64-65 1.2042575742782233 1105 66-67 1.263511064572313 1105 68-69 1.5018078987427579 1105 70-71 1.2863613722649596 1105 72-73 0.5767982424264915 1105 74-75 -0.06507563059601651 1105 76 -1.4633846931414958 1106 1 1.1789137105979322 1106 2 1.044352245818807 1106 3 0.364099670198339 1106 4 -0.4668641504307729 1106 5 -0.03863611509982334 1106 6 -0.011503146892223981 1106 7 0.19694574446101853 1106 8 1.5974056562736934 1106 9 0.4054883510777323 1106 10-11 0.9180192794178126 1106 12-13 0.39700219644826973 1106 14-15 -0.23942252760414107 1106 16-17 -0.03318951026842143 1106 18-19 -0.5212916627372444 1106 20-21 0.5761506627268957 1106 22-23 1.0160828855223158 1106 24-25 0.320835759056024 1106 26-27 0.8358832999022994 1106 28-29 -0.6124406539417677 1106 30-31 -0.13805541162614077 1106 32-33 0.22812828366207327 1106 34-35 0.27991693680291974 1106 36-37 0.9129814338543838 1106 38-39 0.45660460420425153 1106 40-41 0.7915174467010502 1106 42-43 0.9467511681165419 1106 44-45 2.0158111615652103 1106 46-47 0.5079594630317672 1106 48-49 0.9968975996366112 1106 50-51 1.2164942565462766 1106 52-53 0.42688220681696976 1106 54-55 0.2708139430555576 1106 56-57 1.6767798904195779 1106 58-59 0.10365025321512888 1106 60-61 1.5612377712466348 1106 62-63 0.5064818882257249 1106 64-65 1.1335679191058077 1106 66-67 1.4569593404343877 1106 68-69 1.15318720908758 1106 70-71 2.3573958550235794 1106 72-73 1.060246518288558 1106 74-75 0.06802781767984101 1106 76 1.2269601344447096 1107 1 2.2752773469615697 1107 2 1.8207158821824443 1107 3 1.6786451247437952 1107 4 0.7876813041146811 1107 5 0.950454793991085 1107 6 1.1884968531077718 1107 7 1.7387639262791978 1107 8 0.781042019910057 1107 9 -0.7490571034677203 1107 10-11 -0.03925344785491092 1107 12-13 1.5842749237209972 1107 14-15 0.540577472395853 1107 16-17 1.009537762458848 1107 18-19 1.8505265190809368 1107 20-21 1.3061506627268926 1107 22-23 0.5597192491586824 1107 24-25 0.9190175772378453 1107 26-27 0.8213378453568438 1107 28-29 -0.2851679266690468 1107 30-31 1.3410354974647731 1107 32-33 2.2017646472984396 1107 34-35 1.1080987549847379 1107 36-37 0.5629814338543824 1107 38-39 1.334786422386074 1107 40-41 0.7069719921555944 1107 42-43 0.7494784408438164 1107 44-45 0.5821747979288432 1107 46-47 1.3670503721226765 1107 48-49 1.2714430541820647 1107 50-51 1.0992215292735494 1107 52-53 0.5705185704533307 1107 54-55 1.149904852146463 1107 56-57 1.10132534496503 1107 58-59 1.8027411623060416 1107 60-61 0.2785104985193634 1107 62-63 0.3283000700439125 1107 64-65 0.492658828196717 1107 66-67 1.671504794979839 1107 68-69 2.22045993636031 1107 70-71 0.7446685822963097 1107 72-73 -0.12429893625689914 1107 74-75 -0.10379036413833376 1107 76 0.11059649808107253 1108 1 -2.9610862894020684 1108 2 -1.3207324999439045 1108 3 -1.4640359230219993 1108 4 -0.4295760148375507 1108 5 -0.06711069137100978 1108 6 -1.2115031468922268 1108 7 -1.0261051029966097 1108 8 -0.43683163186189944 1108 9 -1.6572235133290434 1108 10-11 -0.9882519070228639 1108 12-13 0.3892055862787771 1108 14-15 -0.47467676489227983 1108 16-17 -1.505562391624352 1108 18-19 -0.5838340356186009 1108 20-21 -0.9972391677815828 1108 22-23 -1.203917114477683 1108 24-25 -1.0550964443338025 1108 26-27 -1.8342861916231286 1108 28-29 0.3231525663972121 1108 30-31 -0.7934791404396933 1108 32-33 -0.12119375023623036 1108 34-35 -0.6812695038750434 1108 36-37 -1.2183744983490072 1108 38-39 -1.4613614974906568 1108 40-41 0.38185642975189893 1108 42-43 -0.930028492900405 1108 44-45 -0.7304600248754696 1108 46-47 -1.055599859002129 1108 48-49 -1.7661532478210162 1108 50-51 -0.0192684553181266 1108 52-53 0.9424754271559479 1108 54-55 -1.1286775823681765 1108 56-57 -0.18372858415669313 1108 58-59 -2.252112458649279 1108 60-61 -0.0013046016347182388 1108 62-63 -0.4043655694013921 1108 64-65 -0.26388970801283307 1108 66-67 -0.6965999815995119 1108 68-69 -0.1508805875225896 1108 70-71 -0.8249770263323519 1108 72-73 -0.7477195834063579 1108 74-75 -0.7338365890998162 1108 76 -0.381853424877324 1109 1 -0.5049459385248767 1109 2 -0.9103845962864554 1109 3 -0.2636196280472731 1109 4 -0.2808992381500701 1109 5 0.1606621305142113 1109 6 0.7534091338095266 1109 7 0.09729662165399589 1109 8 1.8174056562736922 1109 9 0.22829536862159827 1109 10-11 0.8424052443300951 1109 12-13 0.03594956486932688 1109 14-15 0.15987571800989286 1109 16-17 -0.6824877558824554 1109 18-19 -0.35234429431618963 1109 20-21 0.14474715395495963 1109 22-23 -0.12496974605662814 1109 24-25 -0.30442739883871184 1109 26-27 -0.11885354220296307 1109 28-29 0.2138751355319144 1109 30-31 -0.5663010256612253 1109 32-33 0.23637389769716322 1109 34-35 0.08114500697836036 1109 36-37 1.1366656443806988 1109 38-39 0.3043239024498732 1109 40-41 -0.03146500943930164 1109 42-43 0.49429502776566636 1109 44-45 -0.2243642770312846 1109 46-47 0.3544506911019454 1109 48-49 -1.4673129266791776 1109 50-51 0.34052934426557613 1109 52-53 0.8682857155888968 1109 54-55 0.24256832902046455 1109 56-57 0.06783252199852186 1109 58-59 0.23926428830284863 1109 60-61 0.09860619229926826 1109 62-63 -1.6605356556339252 1109 64-65 -0.5655548879117305 1109 66-67 -0.3304090806182458 1109 68-69 0.1598538757542478 1109 70-71 -1.0282181800641403 1109 72-73 -0.23642014837810876 1109 74-75 0.6622383439956323 1109 76 -1.5302328480114298 1110 1 0.2253543885640319 1110 2 -0.18513927960492182 1110 3 -0.07420541454742136 1110 4 -0.4465251673799244 1110 5 -0.33829713204897516 1110 6 1.1613782090399738 1110 7 0.5501660834440685 1110 8 2.3428293850872492 1110 9 -0.7589184285832822 1110 10-11 0.5286972455195098 1110 12-13 -0.1870656001619011 1110 14-15 0.6439673029043362 1110 16-17 0.6385054049858212 1110 18-19 0.25514901522885935 1110 20-21 -0.39554425252734404 1110 22-23 -0.3056120297319218 1110 24-25 0.7415137251577164 1110 26-27 0.08944262193619323 1110 28-29 0.18755934605822944 1110 30-31 0.7149954358314901 1110 32-33 0.9550774362044478 1110 34-35 1.2933067673113996 1110 36-37 0.3748458406340447 1110 38-39 0.29287579064493485 1110 40-41 1.0428733789044387 1110 42-43 0.2733613376080726 1110 44-45 2.193268788683852 1110 46-47 0.5969425138792275 1110 48-49 1.166050142009496 1110 50-51 1.0993756124784824 1110 52-53 0.4424754271559479 1110 54-55 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2.109811314883622 2116 48-49 1.1706013033403124 2116 50-51 1.3653831454351604 2116 52-53 1.3716970216317819 2116 54-55 1.1241472763888893 2116 56-57 0.3875206311603172 2116 58-59 0.9936502532151295 2116 60-61 -0.6265400065311404 2116 62-63 2.044630036373878 2116 64-65 1.518753104291001 2116 66-67 2.218811192286239 2116 68-69 1.733927949828324 2116 70-71 -2.097418959791238 2116 72-73 1.7913576293996698 2116 74-75 -0.5497499600979339 2116 76 -1.3937806062960298 2117 1 -0.17161260519154098 2117 2 -0.12091091207592797 2117 3 0.8065558105492165 2117 4 0.24541655132361484 2117 5 -1.0498641852752613 2117 6 -2.4220294626816994 2117 7 -2.3412998695740725 2117 8 -1.4808399577613969 2117 9 0.9651374738847558 2117 10-11 -1.0084719486523603 2117 12-13 0.18507237188687498 2117 14-15 -1.7611769135690523 2117 16-17 -0.7965228436017568 2117 18-19 -0.194449557474087 2117 20-21 -1.065779161834513 2117 22-23 0.6031004293819677 2117 24-25 0.9148708467753295 2117 26-27 0.8636025981479136 2117 28-29 0.02089267939156514 2117 30-31 -0.6452483940822731 2117 32-33 1.5258475819076907 2117 34-35 0.11623272627660697 2117 36-37 -1.3370185661456162 2117 38-39 0.45344670946741417 2117 40-41 -0.6542720269831648 2117 42-43 -1.6021962003045118 2117 44-45 -0.013837961241812025 2117 46-47 -0.5929177299506883 2117 48-49 -0.2392427512405817 2117 50-51 0.5510556600550487 2117 52-53 0.736706768220472 2117 54-55 -1.6346246534356759 2117 56-57 -0.9497113376505979 2117 58-59 0.8883870953203896 2117 60-61 -0.4715692462972214 2117 62-63 -1.1254479363356786 2117 64-65 -1.355028572122258 2117 66-67 -2.497075747284917 2117 68-69 -0.015584720736978142 2117 70-71 0.8489748023920036 2117 72-73 0.14954476390258975 2117 74-75 1.0657471159254612 2117 76 0.10134609935699146 2118 1 1.2356350220733425 2118 2 1.0007456884417607 2118 3 -0.11032655930985769 2118 4 -0.22424119961110023 2118 5 -0.7389639839522815 2118 6 0.5262017711405633 2118 7 0.28481459692003597 2118 8 -4.166200901103355 2118 9 -1.4027083702337393 2118 10-11 -0.510341376319893 2118 12-13 0.702903835792533 2118 14-15 -0.28073400301397555 2118 16-17 0.4466465553053496 2118 18-19 0.5195280093939019 2118 20-21 -1.9987673700599942 2118 22-23 0.30427960683379496 2118 24-25 0.2762455951216012 2118 26-27 1.1447357589186922 2118 28-29 0.5318216411401977 2118 30-31 -0.2680554116261362 2118 32-33 -0.14383892945267718 2118 34-35 1.4423759531963611 2118 36-37 -0.025543156309552728 2118 38-39 0.028080014040320123 2118 40-41 -0.9942202582169877 2118 42-43 -0.2609537499162471 2118 44-45 1.2474505058275014 2118 46-47 0.9218938892612698 2118 48-49 -0.9224466626584729 2118 50-51 0.5789532729397138 2118 52-53 -0.5947571374453311 2118 54-55 -0.565743433993628 2118 56-57 0.7533372674687548 2118 58-59 0.16578140075611003 2118 60-61 -0.4307294418681167 2118 62-63 0.580908117733923 2118 64-65 0.6081580830402373 2118 66-67 -1.3092701677623353 2118 68-69 1.3374495041695518 2118 70-71 -0.42194840727150407 2118 72-73 -0.7090977440065274 2118 74-75 0.15835568653230325 2118 76 0.7240093147725801 2119 1 0.8147757795634476 2119 2 0.2257315561636375 2119 3 1.2206513943362722 2119 4 0.9365841254312954 2119 5 1.3206742297277643 2119 6 2.2712554737974315 2119 7 -2.1982266693320867 2119 8 1.886371173515073 2119 9 1.8296262821122191 2119 10-11 0.2690537621764335 2119 12-13 0.2759677136896528 2119 14-15 1.2078188517062074 2119 16-17 1.5626725586970949 2119 18-19 2.310087647607581 2119 20-21 0.5854610075544784 2119 22-23 1.899531161384381 2119 24-25 1.2060081728491312 2119 26-27 1.3793315757643612 2119 28-29 2.0410076219202935 2119 30-31 0.5595307952704189 2119 32-33 1.308817938834487 2119 34-35 0.8312962471477476 2119 36-37 0.26642970971645497 2119 38-39 1.0700528800663278 2119 40-41 1.8339312398044925 2119 42-43 0.26123392673722634 2119 44-45 -0.38694745912444617 2119 46-47 0.5165801526869416 2119 48-49 1.8979320823952293 2119 50-51 1.588563222063513 2119 52-53 0.09998565509282287 2119 54-55 0.6184001499521088 2119 56-57 1.8191936835230216 2119 58-59 1.9246847359737487 2119 60-61 2.1339963919362894 2119 62-63 0.8188956813291774 2119 64-65 0.8508092984161593 2119 66-67 1.134200719744733 2119 68-69 0.5276699677082739 2119 70-71 -0.023983455321246794 2119 72-73 -1.0956155506769605 2119 74-75 -0.5823170099063617 2119 76 1.5366153068585042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 44.0 14 133.0 15 215.0 16 359.0 17 456.0 18 434.0 19 475.0 20 386.0 21 302.0 22 283.0 23 234.0 24 241.0 25 247.0 26 328.0 27 330.0 28 427.0 29 485.0 30 603.0 31 710.0 32 955.0 33 1189.0 34 1562.0 35 2440.0 36 3929.0 37 4682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.028169014084504 28.563380281690144 10.286384976525822 28.12206572769953 2 15.121428238474804 25.70269892322752 35.29576283037337 23.880110007924298 3 15.16804176572041 27.338833729548316 31.408194658089776 26.084929846641497 4 11.266489535263133 20.39341816995292 38.19046287232555 30.149629422458396 5 11.569477462359577 36.99249522211346 36.060224677201326 15.37780263832564 6 22.7893534703771 41.90089964107584 18.1559688621638 17.153778026383257 7 22.929194052113925 30.848832331142496 22.56560853959819 23.656365077145388 8 20.65445392252832 34.62452803803664 21.176525427679113 23.54449261175593 9 21.143895958607185 17.31692537174288 22.6355288304666 38.90364983918333 10-11 22.591245979583274 25.418356407029318 27.895865380133316 24.09453223325409 12-13 21.136903929520347 23.290448888267377 26.774810049876475 28.7978371323358 14-15 19.13485293432154 25.989372115788 24.399850836712815 30.475924113177644 16-17 20.971425907798444 27.71640330023773 25.870507621311706 25.441663170652124 18-19 20.512282664429218 26.48114482822915 26.34130424649233 26.665268260849302 20-21 20.223278795506456 27.623176245746517 25.698037570502958 26.455507388244072 22-23 21.607700554700973 26.900666573439615 26.42054724280986 25.07108562904955 24-25 21.30238195124225 28.671980608772667 24.50473127301543 25.520906166969652 26-27 19.869015988439845 28.800167808698085 26.196802312030954 25.13401389083112 28-29 20.945788467813358 27.39710063860532 28.138255721810467 23.51885517177085 30-31 20.344473966345035 27.43905281312637 27.760686151121057 24.455787069407542 32-33 19.510091828648672 28.97263785950683 27.02885377336503 24.488416538479466 34-35 20.892182911480912 28.219829394490283 26.369272362839695 24.51871533118911 36-37 21.176525427679113 27.29688155502727 26.69090570083438 24.835687316459236 38-39 20.52859739896518 27.031184449727313 28.04735934368154 24.392858807625974 40-41 20.48431454808185 26.511443620938795 27.43439146040181 25.56985037057754 42-43 20.32582855544679 26.31566680650725 27.835267794714024 25.523236843331937 44-45 19.358597865100453 26.112897962988857 28.737239546916516 25.791264624994177 46-47 19.56136670861884 26.02666293758449 29.00759800494103 25.404372348855635 48-49 18.745629981820723 26.81210087167296 29.00759800494103 25.434671141565286 50-51 19.013657763482964 27.068475271523795 28.091642194564866 25.82622477042838 52-53 18.267841327553256 27.45536754766233 27.970447023726287 26.306344101058126 54-55 17.87861837505244 27.23162261688342 28.01706055097189 26.87269845709225 56-57 18.214235771220807 27.187339766000097 28.37831538712534 26.220109075653752 58-59 18.596466694634785 27.546263925791266 27.105766093320284 26.75150328625367 60-61 18.393697851116393 26.830746282571198 27.58355474758775 27.19200111872465 62-63 18.822542301775975 26.19913298839323 27.366801845895676 27.61152286393511 64-65 18.955390854425954 26.89367454435277 26.907658602526453 27.24327599869482 66-67 19.479793035939032 27.0917820351466 25.737659068661728 27.690765860252647 68-69 19.405211392346057 27.581224071225467 25.639770661445954 27.37379387498252 70-71 19.53805994499604 28.301403067170096 25.27385447256794 26.88668251526593 72-73 20.218617442781895 27.122080827856244 25.41136437794248 27.24793735141938 74-75 19.05094858527945 28.271104274460445 25.88449167948539 26.793455460774716 76 19.34927515965133 27.352817787721996 25.67939215960472 27.618514893021956 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 0.0 12 0.0 13 0.5 14 2.0 15 2.5 16 2.0 17 2.0 18 1.5 19 2.5 20 3.0 21 2.0 22 10.0 23 21.5 24 23.5 25 22.0 26 41.5 27 66.5 28 90.0 29 108.0 30 135.0 31 197.5 32 270.0 33 307.0 34 342.0 35 480.5 36 574.5 37 565.0 38 713.5 39 983.5 40 1179.0 41 1286.5 42 1320.0 43 1435.0 44 1579.5 45 1646.0 46 1683.0 47 1660.0 48 1577.5 49 1448.5 50 1379.0 51 1217.0 52 975.0 53 804.5 54 714.0 55 683.0 56 544.0 57 373.5 58 311.0 59 278.0 60 208.0 61 152.0 62 133.0 63 116.0 64 81.0 65 63.5 66 51.0 67 38.0 68 39.5 69 26.5 70 12.0 71 12.0 72 14.0 73 10.5 74 4.0 75 3.0 76 2.5 77 1.5 78 2.0 79 3.0 80 2.0 81 2.0 82 2.0 83 1.0 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7131869668577822 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 21453.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.49116673658695 #Duplication Level Percentage of deduplicated Percentage of total 1 79.76136141357502 57.02232787955065 2 10.979983047532112 15.699435976320327 3 4.668448849188238 10.012585652356314 4 2.1386190258851143 6.115694774623596 5 1.0823498728564909 3.868922761385354 6 0.6063767359979135 2.6010348203048523 7 0.35860989763317463 1.794620798955857 8 0.1564843189672035 0.8949797231156482 9 0.07824215948360175 0.503426094252552 >10 0.16952467888113712 1.486971519134853 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT 25 0.11653381811401668 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.013984058173682002 0.0 6 0.0 0.0 0.0 0.013984058173682002 0.0 7 0.0 0.0 0.0 0.01864541089824267 0.0 8 0.0 0.0 0.0 0.023306763622803338 0.0 9 0.0 0.0 0.0 0.04195217452104601 0.0 10 0.0 0.0 0.0 0.060597585419288676 0.0 11 0.0 0.0 0.0 0.06525893814384935 0.0 12 0.0 0.0 0.0 0.06525893814384935 0.0 13 0.0 0.0 0.0 0.07924299631753134 0.0 14 0.0 0.0 0.0 0.08390434904209201 0.0 15 0.0 0.0 0.0 0.08856570176665268 0.0 16 0.0 0.0 0.0 0.10254975994033469 0.0 17 0.0 0.0 0.0 0.10254975994033469 0.0 18 0.0 0.0 0.0 0.11187246538945601 0.0 19 0.0 0.0 0.0 0.13984058173682 0.0 20 0.0 0.0 0.0 0.14916328718594135 0.0 21 0.0 0.0 0.0 0.17713140353330536 0.0 22 0.0 0.0 0.0 0.20509951988066938 0.0 23 0.0 0.0 0.0 0.20976087260523005 0.0 24 0.0 0.0 0.0 0.21442222532979072 0.0 25 0.0 0.0 0.0 0.21442222532979072 0.0 26 0.0 0.0 0.0 0.2284062835034727 0.0 27 0.0 0.0 0.0 0.24705169440171537 0.0 28 0.0 0.0 0.0 0.2610357525753974 0.0 29 0.0 0.0 0.0 0.26569710529995805 0.0 30 0.0 0.0 0.0 0.29366522164732206 0.0 31 0.0 0.0 0.0 0.3029879270964434 0.0 32 0.0 0.0 0.0 0.3449401016174894 0.0 33 0.0 0.0 0.0 0.40553768703677806 0.0 34 0.0 0.0 0.0 0.4568125670069454 0.0 35 0.0 0.0 0.0 0.5826690905700834 0.0 36 0.0 0.0 0.0 0.7318323777560248 0.0 37 0.0 0.0 0.0 1.1047405957208782 0.0 38 0.0 0.0 0.0 1.4776488136857315 0.0 39 0.0 0.0 0.0 1.962429497040041 0.0 40 0.0 0.0 0.0 2.5730667039574886 0.0 41 0.0 0.0 0.0 3.3561739616836808 0.0 42 0.0 0.0 0.0 4.1066517503379485 0.0 43 0.0 0.0 0.0 4.7545797790518805 0.0 44 0.0 0.0 0.0 5.5003962149815875 0.0 45 0.0 0.0 0.0 5.985176898335897 0.0 46 0.0 0.0 0.0 6.665734396121755 0.0 47 0.0 0.0 0.0 7.430196242949704 0.0 48 0.0 0.0 0.0 8.367128140586399 0.0 49 0.0 0.0 0.0 8.959119936605603 0.0 50 0.0 0.0 0.0 9.555773085349369 0.0 51 0.0 0.0 0.0 10.306250874003636 0.0 52 0.0 0.0 0.0 10.944856197268447 0.0 53 0.0 0.0 0.0 11.438959586071878 0.0 54 0.0 0.0 0.0 12.114855731133176 0.0 55 0.0 0.0 0.0 12.49708665454715 0.0 56 0.0 0.0 0.0 13.06577168694355 0.0 57 0.0 0.0 0.0 13.723022421106606 0.0 58 0.0 0.0 0.0 14.296368806227568 0.0 59 0.0 0.0 0.0 14.851069780450286 0.0 60 0.0 0.0 0.0 15.335850463804595 0.0 61 0.0 0.0 0.0 15.909196848925559 0.0 62 0.0 0.0 0.0 16.47788188132196 0.0 63 0.0 0.0 0.0 16.96732391740083 0.0 64 0.0 0.0 0.0 17.447443248030577 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTATAC 20 7.752867E-5 70.00001 3 TAAGACT 15 0.0021951767 70.0 9 CTCTATA 15 0.0021951767 70.0 2 TTCACCA 20 0.0068598553 52.500004 15 TGACTTC 20 0.0068598553 52.500004 22 CCAGTCA 20 0.0068598553 52.500004 19 GTACATG 20 0.0068598553 52.500004 1 GCGAACT 20 0.0068598553 52.500004 28 CTGACTT 20 0.0068598553 52.500004 21 TACATGC 20 0.0068598553 52.500004 2 GAACTAC 20 0.0068598553 52.500004 30 GTAAGTA 20 0.0068598553 52.500004 16 AGACTTC 20 0.0068598553 52.500004 11 AAAGCGA 20 0.0068598553 52.500004 25 GACTTCC 20 0.0068598553 52.500004 23 AGCGAAC 20 0.0068598553 52.500004 27 ACCAGTC 20 0.0068598553 52.500004 18 ACTATAA 20 0.0068598553 52.500004 3 ATCCTTC 20 0.0068598553 52.500004 50 TACTATA 40 0.0023670676 35.000004 2 >>END_MODULE