FastQCFastQC Report
Wed 25 May 2016
SRR1780650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21453
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT8283.8596000559362325No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT3491.626812100871673Illumina PCR Primer Index 10 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1260.5873304432946441No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC660.3076492798210041No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG530.24705169440171537No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC520.24239034167715473Illumina PCR Primer Index 10 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT310.1445019344613807Illumina PCR Primer Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCGT300.13984058173682No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT230.10721111266489534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACT150.002195176770.08
ACCTGCC200.006859855352.50000436
TTGAAAA1950.035.89743458
CTTGAAA1950.035.89743457
GCTTGAA2100.035.056
CTGCTTG2150.034.18604754
TGAAAAA1950.034.10256659
TATGCCG2200.033.4090943
GAAAAAA2200.033.4090960
CCGTCTT2200.033.4090947
ATGCCGT2200.033.4090944
TCTTCTG2200.033.4090950
AGATTAT2200.033.4090932
CGTCTTC2200.033.4090948
TGCCGTC2250.032.66666845
TCTGCTT2250.032.66666853
GTCTTCT2250.032.66666849
GCCGTCT2250.032.66666846
TGCTTGA2150.032.5581455
CGTATGC2400.032.08333241