##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780650_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21453 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.0683820444693 34.0 34.0 34.0 32.0 34.0 2 33.38768470610171 34.0 34.0 34.0 33.0 34.0 3 33.5575443993847 34.0 34.0 34.0 34.0 34.0 4 33.55647228825805 34.0 34.0 34.0 34.0 34.0 5 33.56215913858202 34.0 34.0 34.0 34.0 34.0 6 37.335244487950405 38.0 38.0 38.0 37.0 38.0 7 36.9798629562299 38.0 37.0 38.0 35.0 38.0 8 37.01710716449914 38.0 37.0 38.0 36.0 38.0 9 36.83004707966252 38.0 37.0 38.0 34.0 38.0 10-11 36.52871393278329 38.0 37.0 38.0 34.0 38.0 12-13 36.40866079336223 38.0 37.0 38.0 34.0 38.0 14-15 36.62534377476344 38.0 37.0 38.0 34.0 38.0 16-17 36.74910268960052 38.0 37.0 38.0 34.5 38.0 18-19 36.733720225609474 38.0 37.0 38.0 34.0 38.0 20-21 36.50874003635855 38.0 37.0 38.0 34.0 38.0 22-23 36.56395375938097 38.0 37.0 38.0 34.0 38.0 24-25 35.91702792150282 38.0 36.5 38.0 32.5 38.0 26-27 36.113014496806976 38.0 37.0 38.0 33.0 38.0 28-29 36.04514520113737 38.0 37.0 38.0 33.5 38.0 30-31 35.74208735375006 38.0 36.5 38.0 31.5 38.0 32-33 36.198503705775416 38.0 37.0 38.0 34.0 38.0 34-35 36.183890364983924 38.0 37.0 38.0 34.0 38.0 36-37 36.29126462499417 38.0 37.0 38.0 34.0 38.0 38-39 35.79194052113924 38.0 36.5 38.0 32.5 38.0 40-41 35.548617908917166 38.0 36.0 38.0 31.0 38.0 42-43 35.949517549993004 38.0 36.5 38.0 32.5 38.0 44-45 36.17943877313196 38.0 37.0 38.0 34.0 38.0 46-47 36.434018552183844 38.0 37.0 38.0 34.0 38.0 48-49 36.19827063813919 38.0 37.0 38.0 34.0 38.0 50-51 36.33004707966252 38.0 37.0 38.0 34.0 38.0 52-53 36.217172423437276 38.0 37.0 38.0 34.0 38.0 54-55 36.4283550086235 38.0 37.0 38.0 34.0 38.0 56-57 36.45469165151727 38.0 37.0 38.0 34.0 38.0 58-59 36.44965739057474 38.0 37.0 38.0 34.0 38.0 60-61 36.157949937071734 38.0 37.0 38.0 33.0 38.0 62-63 36.46135738591339 38.0 37.0 38.0 34.0 38.0 64-65 36.472894233906686 38.0 37.0 38.0 34.0 38.0 66-67 35.78441243648906 38.0 37.0 38.0 31.5 38.0 68-69 35.04339719386566 38.0 36.0 38.0 28.0 38.0 70-71 34.79289609844777 38.0 36.0 38.0 25.5 38.0 72-73 34.26070945788467 38.0 34.5 38.0 23.5 38.0 74-75 34.68943737472615 38.0 36.0 38.0 24.5 38.0 76 34.20575210926211 37.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0017875026559011076 1101 2 0.3955599805155714 1101 3 0.022174762505201784 1101 4 0.29557745429651305 1101 5 0.331631753354813 1101 6 -1.2076095717927728 1101 7 0.7848849574561498 1101 8 0.4600287691693694 1101 9 0.480864568054848 1101 10-11 0.20519555470458073 1101 12-13 0.504032805941101 1101 14-15 0.9422390760071124 1101 16-17 -0.23926600868906434 1101 18-19 -0.6697450159165044 1101 20-21 -0.1275040070645872 1101 22-23 -0.13927734471904785 1101 24-25 -1.6774664691359789 1101 26-27 -0.802937155471497 1101 28-29 -0.5077516239402868 1101 30-31 0.8534860562879913 1101 32-33 0.10828320696516158 1101 34-35 0.13333173489616001 1101 36-37 -0.3041244185063974 1101 38-39 -0.48297587103223805 1101 40-41 0.5999695862779433 1101 42-43 0.13958949244920404 1101 44-45 -0.39056885578843037 1101 46-47 0.7610277391089753 1101 48-49 -1.513821016922229 1101 50-51 -0.9758952495271842 1101 52-53 -1.5192809893109214 1101 54-55 -1.7163673509479338 1101 56-57 -1.1728935213942009 1101 58-59 -1.7894683821761959 1101 60-61 -1.8300407750791123 1101 62-63 -2.1204585724770624 1101 64-65 -1.5388793905433928 1101 66-67 -2.031602785421292 1101 68-69 -1.447268927855525 1101 70-71 -0.7962649827450221 1101 72-73 -2.2321188121712225 1101 74-75 0.4487669255982141 1101 76 0.6297402272194006 1102 1 -1.276702525834125 1102 2 0.25524659020217655 1102 3 9.499042543126279E-5 1102 4 0.28133244005150004 1102 5 0.5431702148932729 1102 6 0.2902536760704777 1102 7 -0.389616467045272 1102 8 0.10960141874202378 1102 9 0.37473921192949433 1102 10-11 0.7522041017131329 1102 12-13 0.7985484754567764 1102 14-15 -0.20270394393590863 1102 16-17 0.7806770112539567 1102 18-19 -0.04403276520424981 1102 20-21 0.821570067009489 1102 22-23 0.3824463020046096 1102 24-25 -4.15681119848071 1102 26-27 -0.527296129830475 1102 28-29 0.36261874643007985 1102 30-31 1.3862495890515305 1102 32-33 0.5516592753412297 1102 34-35 0.8879613645257933 1102 36-37 -1.7556913700733503 1102 38-39 0.4557705677142039 1102 40-41 0.9375764238847779 1102 42-43 1.1331792360389557 1102 44-45 1.1845735943540134 1102 46-47 1.389945118026354 1102 48-49 -2.701142954244169 1102 50-51 -0.6696274432593725 1102 52-53 -0.10831232834226512 1102 54-55 0.9054275208469278 1102 56-57 1.1034597549590686 1102 58-59 -0.21147692918474092 1102 60-61 -6.37633707137541 1102 62-63 -2.42387737589587 1102 64-65 0.20684710518310823 1102 66-67 1.1703202915017883 1102 68-69 -1.36179884238544 1102 70-71 -0.7813077177877545 1102 72-73 -5.709682914735325 1102 74-75 -1.974666122834833 1102 76 -1.4300888326096555 1103 1 0.2481817334251346 1103 2 0.2477234420540313 1103 3 -1.136479083648645 1103 4 -0.47966293031887375 1103 5 0.21504521489327288 1103 6 -0.18717687948507944 1103 7 0.6827214959176899 1103 8 0.5609903076309095 1103 9 0.1311049526702348 1103 10-11 -0.07244867606464567 1103 12-13 -1.0987316171358188 1103 14-15 -1.0415349624544277 1103 16-17 -0.7086169702275242 1103 18-19 0.23229825331426923 1103 20-21 -1.0884415070645872 1103 22-23 -0.17773888318058084 1103 24-25 -4.635399161443672 1103 26-27 -1.4778169631638036 1103 28-29 -1.4765016239402868 1103 30-31 0.44122644090337815 1103 32-33 1.0247495531190083 1103 34-35 0.19583173489616001 1103 36-37 -1.1526821108140908 1103 38-39 -0.8555720248783913 1103 40-41 -1.1830832983374435 1103 42-43 -0.7912999306277158 1103 44-45 0.7632772980577229 1103 46-47 0.37460946987820165 1103 48-49 -3.1887008246145356 1103 50-51 -0.9488519802964106 1103 52-53 -0.18093964315707467 1103 54-55 -1.0607183124864008 1103 56-57 -0.305105059855741 1103 58-59 -1.0743241514069624 1103 60-61 -4.314415775079112 1103 62-63 -1.4299537647847558 1103 64-65 -0.44332650592800604 1103 66-67 -0.7828047084982117 1103 68-69 -0.05363912016321848 1103 70-71 -0.07210632889886881 1103 72-73 -4.649787081401989 1103 74-75 -1.9448628820940925 1103 76 -4.032519388165213 1104 1 0.5439602421970662 1104 2 -0.09465594391087961 1104 3 0.39093319705310847 1104 4 -0.29079231628378466 1104 5 0.36773161840204693 1104 6 0.40136478718158486 1104 7 0.5821184257422516 1104 8 0.910771009385293 1104 9 0.9926729351263717 1104 10-11 -0.21855613220500203 1104 12-13 0.9252541284782154 1104 14-15 0.7743915726332915 1104 16-17 0.34305517889528403 1104 18-19 0.627994525244091 1104 20-21 0.05012757188278272 1104 22-23 0.41217339752117255 1104 24-25 1.226717066626506 1104 26-27 1.0384933438537445 1104 28-29 1.0624238146562064 1104 30-31 0.35213652862267963 1104 32-33 -0.4688085609160737 1104 34-35 -0.8688612475599768 1104 36-37 0.5167257839227517 1104 38-39 0.8368621856479237 1104 40-41 1.7592138507853647 1104 42-43 0.38317923603894855 1104 44-45 0.2479264208647436 1104 46-47 0.8558320575975031 1104 48-49 -0.14168876321102886 1104 50-51 0.2933355197035894 1104 52-53 -0.032776265964088225 1104 54-55 0.21244506470658564 1104 56-57 -0.005214708978549254 1104 58-59 -0.916155291757839 1104 60-61 0.20066097930685345 1104 62-63 0.12583022644331265 1104 64-65 0.9466131870544459 1104 66-67 0.3238290634316101 1104 68-69 1.4700725026438022 1104 70-71 1.6207390658379737 1104 72-73 0.9087381378962576 1104 74-75 -0.25585520665549666 1104 76 -1.3189777214985483 1105 1 0.5860050092872058 1105 2 0.3484777523988569 1105 3 0.046710571523767896 1105 4 -0.0717965510085321 1105 5 -0.28441599200328227 1105 6 0.1312498446528494 1105 7 0.7974844269521739 1105 8 -0.8290958992656456 1105 9 0.3396178837047188 1105 10-11 0.7244263239353543 1105 12-13 -0.5699061861013348 1105 14-15 0.520156847890398 1105 16-17 -0.3387355047102858 1105 18-19 -0.016893557030556394 1105 20-21 0.23887530328023843 1105 22-23 -0.3113595728357552 1105 24-25 0.13884652821150212 1105 26-27 -0.10200877350862925 1105 28-29 0.22500699674936442 1105 30-31 0.6001704064206166 1105 32-33 0.5621956738086595 1105 34-35 -0.8968406788969432 1105 36-37 -0.28091486943477406 1105 38-39 -0.6195806455680426 1105 40-41 -0.21568028109606274 1105 42-43 -0.7748667409725414 1105 44-45 0.19000143598875496 1105 46-47 1.0780038664299312 1105 48-49 -0.7353064280628132 1105 50-51 0.9054044852208278 1105 52-53 0.07687285684292533 1105 54-55 0.3134734978584248 1105 56-57 0.09036907807529104 1105 58-59 0.6525077451447672 1105 60-61 0.5492695697484749 1105 62-63 -0.38173178202613656 1105 64-65 -0.11978124730731565 1105 66-67 -0.7779555705671797 1105 68-69 -0.7516455856804569 1105 70-71 1.0213742745494088 1105 72-73 0.2615814530807725 1105 74-75 -0.5873736579561566 1105 76 0.18676940493823224 1106 1 -2.0974432665748637 1106 2 0.04709844205402902 1106 3 0.2660209163513585 1106 4 0.41096206968112625 1106 5 0.3031702148932709 1106 6 0.47469812051492255 1106 7 -0.5066535040823084 1106 8 0.13849030763091008 1106 9 -0.3245200473297629 1106 10-11 0.4344263239353552 1106 12-13 0.5459558828641846 1106 14-15 -0.321222462454422 1106 16-17 0.28919552977247065 1106 18-19 0.06448575331427264 1106 20-21 0.027495992935413938 1106 22-23 0.5172611168194194 1106 24-25 -0.6297741614436703 1106 26-27 0.549370536836193 1106 28-29 0.03224837605971942 1106 30-31 0.6381014409033838 1106 32-33 0.5494370531190071 1106 34-35 1.2183317348961609 1106 36-37 0.7335678891859132 1106 38-39 -0.556822024878393 1106 40-41 0.7053542016625514 1106 42-43 0.7965125693722825 1106 44-45 1.2382772980577244 1106 46-47 0.6317969698782022 1106 48-49 1.2484866753854647 1106 50-51 0.3133355197035854 1106 52-53 -0.2931271431570792 1106 54-55 -0.18790581248640592 1106 56-57 0.558644940144255 1106 58-59 0.5063008485930354 1106 60-61 0.9599592249208868 1106 62-63 1.157233735215243 1106 64-65 -1.114264005928007 1106 66-67 0.8003202915017908 1106 68-69 1.6704233798367838 1106 70-71 0.5675811711011249 1106 72-73 1.2436504185980084 1106 74-75 0.41607461790590605 1106 76 0.23435561183478626 1107 1 0.36437491524331733 1107 2 0.1707348056903939 1107 3 -0.17397908364864634 1107 4 0.32005297877203276 1107 5 -0.038647966924905575 1107 6 0.48015266596946304 1107 7 0.6915283140995072 1107 8 0.3439448530854534 1107 9 -0.011792774602490397 1107 10-11 0.7653354148444507 1107 12-13 0.8877740646823682 1107 14-15 1.0560502648182961 1107 16-17 0.762831893408837 1107 18-19 0.7244857533142692 1107 20-21 0.7884050838445091 1107 22-23 0.25635202591033135 1107 24-25 0.7393167476472371 1107 26-27 -0.556084008618349 1107 28-29 0.8558847396960729 1107 30-31 0.8471923499942875 1107 32-33 0.9748915985735493 1107 34-35 0.1628771894416161 1107 36-37 0.9899315255495438 1107 38-39 0.36317797512160865 1107 40-41 0.45171783802619103 1107 42-43 1.0346943875541044 1107 44-45 0.542822752603179 1107 46-47 0.9690696971509283 1107 48-49 0.8466684935672859 1107 50-51 1.2842446106126815 1107 52-53 0.8086910386611095 1107 54-55 0.9539123693317819 1107 56-57 0.538644940144259 1107 58-59 1.148119030411216 1107 60-61 1.4199592249208877 1107 62-63 0.6872337352152442 1107 64-65 0.7684632667992659 1107 66-67 0.6566839278654228 1107 68-69 1.7922415616549614 1107 70-71 1.4484902620102247 1107 72-73 1.7554686004161937 1107 74-75 0.13425643608773186 1107 76 1.7234465209256982 1108 1 -0.4581212326765609 1108 2 0.23421708612183068 1108 3 -0.03160620229271416 1108 4 0.32621630696926474 1108 5 0.17028885896107226 1108 6 0.22622354424373725 1108 7 0.07334649591768994 1108 8 -1.035407997453838 1108 9 -1.0031641151263742 1108 10-11 0.19476530698620564 1108 12-13 0.8415491032031639 1108 14-15 0.13894702907099798 1108 16-17 -1.031312944803794 1108 18-19 0.3431298211108782 1108 20-21 0.8195298912404994 1108 22-23 0.06590518461602812 1108 24-25 0.6810732961834418 1108 26-27 -0.2546972597739767 1108 28-29 -0.1941923019063907 1108 30-31 -0.024610423503403922 1108 32-33 0.6429963751529044 1108 34-35 0.8473147857436203 1108 36-37 1.0756017874909958 1108 38-39 0.634364415799574 1108 40-41 1.4272186084422174 1108 42-43 -0.9445043797802555 1108 44-45 0.676412891278062 1108 46-47 0.5045088342849837 1108 48-49 0.8267917601312291 1108 50-51 0.5390982315679906 1108 52-53 -0.3510932448519952 1108 54-55 -0.504176998927079 1108 56-57 -0.5206770937540455 1108 58-59 -0.2472584734408585 1108 60-61 0.4453829537344447 1108 62-63 0.3143523792830365 1108 64-65 0.08726141780081065 1108 66-67 1.2508287660780582 1108 68-69 0.5087284645825463 1108 70-71 -1.5153001848310694 1108 72-73 -0.9060105983511377 1108 74-75 -1.636467754975449 1108 76 -0.4771698118940293 1109 1 -0.9998994069257421 1109 2 -0.0420243649635097 1109 3 -0.1529264520696998 1109 4 0.3056989117863864 1109 5 0.13966144296345107 1109 6 -0.21267030053770952 1109 7 -0.27753069706476907 1109 8 0.20901662342038207 1109 9 0.6417957421439198 1109 10-11 0.41302281516342276 1109 12-13 0.9077102688290921 1109 14-15 0.8270231515806543 1109 16-17 0.5798972841584344 1109 18-19 0.627994525244091 1109 20-21 1.1817065192512004 1109 22-23 0.526208485240474 1109 24-25 0.44601531224054014 1109 26-27 0.573581063151984 1109 28-29 0.10628346377901465 1109 30-31 1.2205575812542548 1109 32-33 0.811893193469885 1109 34-35 -0.23728230019155916 1109 36-37 0.4465503453262585 1109 38-39 0.880721834770732 1109 40-41 1.1451787630660633 1109 42-43 1.5673897623547361 1109 44-45 0.835645719110353 1109 46-47 1.0400425839132836 1109 48-49 0.7618200087188001 1109 50-51 0.35473902847552097 1109 52-53 0.2216096989481855 1109 54-55 -0.5331689703811406 1109 56-57 0.41583792260039587 1109 58-59 -0.2582605549157293 1109 60-61 1.2532925582542163 1109 62-63 0.46793548960121 1109 64-65 0.8939816081070759 1109 66-67 0.03435537922108978 1109 68-69 -0.3808046903386568 1109 70-71 -0.4582083025830812 1109 72-73 0.6192644536857301 1109 74-75 -0.09796046981339401 1109 76 0.9792678925365408 1110 1 -0.06829072420198656 1110 2 -0.07086765964088215 1110 3 0.39212261126660763 1110 4 0.309267154426891 1110 5 0.4923227572661517 1110 6 0.4635116798369552 1110 7 0.8191092077820983 1110 8 0.6595072567834492 1110 9 0.5900562238566778 1110 10-11 0.42357886630823316 1110 12-13 1.0788372387963818 1110 14-15 1.0542012663591365 1110 16-17 0.4686870551961988 1110 18-19 -0.2924633992280974 1110 20-21 0.743258704799814 1110 22-23 -0.5527388831805808 1110 24-25 -0.3443504326301081 1110 26-27 0.6690315537853451 1110 28-29 0.5854687150427651 1110 30-31 0.24657601717456146 1110 32-33 0.7446912904071468 1110 34-35 1.076128345065655 1110 36-37 1.0162797535926913 1110 38-39 -0.1113982960648272 1110 40-41 -0.1914254593543916 1110 42-43 0.42837697615194514 1110 44-45 0.3713281455153492 1110 46-47 0.5468817156409145 1110 48-49 1.301368031317672 1110 50-51 -0.37615600572013363 1110 52-53 0.55568641616496 1110 54-55 -0.03807530401182646 1110 56-57 1.0216957876018853 1110 58-59 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2116 48-49 0.2710792679780596 2116 50-51 -0.8270348506667773 2116 52-53 0.14168767165773488 2116 54-55 -0.48346136804195794 2116 56-57 -0.23913283763351956 2116 58-59 -1.748513966221772 2116 60-61 -1.5337444787828218 2116 62-63 0.094641142622649 2116 64-65 -2.1450047466687465 2116 66-67 0.1332832544647502 2116 68-69 -1.0192062497928518 2116 70-71 0.8575811711011312 2116 72-73 -0.3207940258464319 2116 74-75 -1.1968883450570544 2116 76 -1.5411999437207697 2117 1 0.7018549790391688 2117 2 0.46674756486105196 2117 3 0.26812617950925244 2117 4 -0.1153537197925587 2117 5 0.12211758331432776 2117 6 0.43645250647983147 2117 7 0.38913596960190233 2117 8 0.19147276377125877 2117 9 -0.6213621525929227 2117 10-11 -0.5782052550120156 2117 12-13 -1.3291318364340654 2117 14-15 0.02877753754557233 2117 16-17 -0.6130851719819077 2117 18-19 -0.8281458256331007 2117 20-21 -0.3358373403979229 2117 22-23 0.5612962045387206 2117 24-25 -1.9311776702156038 2117 26-27 -2.0579978842164337 2117 28-29 -1.7972253081508143 2117 30-31 0.02757512511389848 2117 32-33 -2.76705417495117 2117 34-35 0.39429664717685853 2117 36-37 -0.99204614590181 2117 38-39 -0.1280500950538297 2117 40-41 0.22412613148711813 2117 42-43 1.348091516740709 2117 44-45 0.10757554367175715 2117 46-47 -0.26697495994636 2117 48-49 -0.5013378860180424 2117 50-51 0.7582478004053428 2117 52-53 0.6338904007025761 2117 54-55 0.09840997698728415 2117 56-57 0.4509256418986425 2117 58-59 -0.8986114321087157 2117 60-61 -0.615128494377359 2117 62-63 -0.05838029987247495 2117 64-65 -0.9481236550508143 2117 66-67 -0.31652181376136923 2117 68-69 0.1893707482578364 2117 70-71 -0.39680479381114964 2117 72-73 0.8561065589488877 2117 74-75 1.5423904073795924 2117 76 1.6810222785014517 2118 1 0.35960591375300055 2118 2 0.1316886059884581 2118 3 -0.26250367381258144 2118 4 0.18145387295981408 2118 5 0.5103833296473681 2118 6 -0.8882526991572135 2118 7 0.8438382991963778 2118 8 0.5230804715653363 2118 9 -1.3763233260182872 2118 10-11 -0.36163924983514306 2118 12-13 -0.7776506745128628 2118 14-15 0.6271381932832796 2118 16-17 -2.0122798800635877 2118 18-19 -0.3984650663578577 2118 20-21 -0.06807777755638966 2118 22-23 0.5784086578030241 2118 24-25 1.228586494294035 2118 26-27 -2.9876786434916767 2118 28-29 0.05782214655151563 2118 30-31 -0.07337396893268533 2118 32-33 -0.03941540589738679 2118 34-35 0.4263645217814087 2118 36-37 -1.7792189960599956 2118 38-39 -0.07944497569805975 2118 40-41 0.5842066606789516 2118 42-43 1.0525781431427745 2118 44-45 -1.3412308986635892 2118 46-47 0.3647477895503357 2118 48-49 0.6825850360412034 2118 50-51 -0.5099431688209961 2118 52-53 -0.6977173070915015 2118 54-55 -0.5426599108470498 2118 56-57 0.3993006778491761 2118 58-59 -0.13861718419384772 2118 60-61 -0.6947948734397684 2118 62-63 -0.3291597074077046 2118 64-65 0.08753927276051598 2118 66-67 -0.20263052817033866 2118 68-69 -0.3485930136058428 2118 70-71 0.054302482576545685 2118 72-73 0.39824058253243777 2118 74-75 0.27017297856164646 2118 76 0.8996015134741313 2119 1 0.41359121618375383 2119 2 -0.41014293725631035 2119 3 0.21912436462721985 2119 4 -0.14076206824990578 2119 5 -0.38786426786534633 2119 6 -0.29978463810576983 2119 7 0.1767947717797611 2119 8 0.42952479038952873 2119 9 0.16720409060126684 2119 10-11 0.19856425496983832 2119 12-13 0.25768002079521324 2119 14-15 0.24429477892488194 2119 16-17 0.5233334608069526 2119 18-19 0.2589685119349596 2119 20-21 -0.1318143518921744 2119 22-23 0.41277835819872877 2119 24-25 1.1388465282115021 2119 26-27 0.932473985112054 2119 28-29 0.8888001001976491 2119 30-31 1.4018945443516486 2119 32-33 0.8208163634638339 2119 34-35 0.8445386314478895 2119 36-37 0.12425754435832204 2119 38-39 -0.05923581798183619 2119 40-41 0.6463886844211757 2119 42-43 0.46651256937228425 2119 44-45 0.5262083325404845 2119 46-47 0.04352110780923368 2119 48-49 0.23883150297167077 2119 50-51 1.0433355197035894 2119 52-53 0.3441142361532741 2119 54-55 0.5289907392377415 2119 56-57 -0.7716998874419474 2119 58-59 -0.2009405307173111 2119 60-61 0.7647868111277845 2119 62-63 0.36826821797386344 2119 64-65 0.3888394423478587 2119 66-67 1.0323892570190267 2119 68-69 1.6276647591471303 2119 70-71 0.7627535848942344 2119 72-73 2.106409039287662 2119 74-75 0.5764194454921139 2119 76 0.5488383704554778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 2.0 23 3.0 24 7.0 25 17.0 26 51.0 27 73.0 28 108.0 29 207.0 30 325.0 31 497.0 32 758.0 33 1248.0 34 1817.0 35 2735.0 36 4585.0 37 9019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.69691884584907 23.94070759334359 10.860951848226355 31.501421712580992 2 15.424416165571248 18.869155829021583 42.66070013517923 23.04572787022794 3 15.50365916188878 25.861184915862584 33.51046473686664 25.124691185381998 4 11.499557171491166 22.989791637533212 38.24639910502028 27.26425208595534 5 11.592784225982381 37.00181792756258 38.61930732298513 12.786090523469912 6 24.10385493870321 42.81452477508973 18.1792756257866 14.902344660420455 7 23.358038502773503 30.904768563837226 23.38600661912087 22.3511863142684 8 21.209154896751038 38.47946674124831 20.60784039528271 19.703537966717942 9 20.924812380552837 14.893021954971333 21.913019158159695 42.26914650631613 10-11 22.794014823101662 26.560387824546684 28.898056215913858 21.747541136437796 12-13 22.421106605136814 21.63799934741062 28.142917074535028 27.797976972917542 14-15 19.796765021209158 24.02927329511024 23.23917400829721 32.9347876753834 16-17 23.1925604810516 28.84678133594369 23.833496480678694 24.127161702326013 18-19 23.32773971006386 26.75150328625367 24.723814851069783 25.19694215261269 20-21 21.111266489535264 26.192140959306393 24.003635855125157 28.69295669603319 22-23 21.241784365822962 28.688295343308628 25.252878385307415 24.817041905560995 24-25 23.397660000932273 29.725446324523375 24.27399431314968 22.602899361394677 26-27 23.33706241551298 30.489908171351328 24.600289003868923 21.572740409266768 28-29 20.645131217079197 29.38050622290589 26.553395795459846 23.420966764555075 30-31 23.25548874283317 27.487997016734255 27.828275765627186 21.42823847480539 32-33 22.68214235771221 28.57642287791917 27.24094532233254 21.500489442036077 34-35 20.892182911480912 29.53899221554095 29.39915163380413 20.169673239174006 36-37 20.458677108096772 28.049690020043816 29.091502353983127 22.40013051787629 38-39 21.174194751316833 26.024332261222206 27.893534703771035 24.907938283689926 40-41 19.554374679532 25.283177178017063 30.10534657157507 25.05710157087587 42-43 23.1692537174288 24.409173542161934 30.422318556845195 21.99925418356407 44-45 19.689553908544262 26.714212464457187 29.650864680930404 23.94536894606815 46-47 21.903696452710577 24.183097935020744 28.683633990584067 25.229571621684617 48-49 21.614692583787814 23.642381018971705 28.541462732484966 26.201463664755515 50-51 19.302661632405723 24.082878851442686 30.296462033282058 26.317997482869526 52-53 18.55684519647602 24.86132475644432 33.55707826411224 23.024751782967417 54-55 17.519694215261268 24.95688248729781 31.601640796159046 25.92178250128187 56-57 20.971425907798444 24.65622523656365 28.606721670628815 25.765627185009087 58-59 18.51256234559269 26.504451591851957 31.57600335617396 23.406982706381392 60-61 20.633477835267794 29.45974921922342 26.206125017480076 23.700647928028715 62-63 17.799375378734908 33.24010627884212 24.681862676548736 24.27865566587424 64-65 17.87395702232788 34.06982706381392 24.562998182072437 23.493217731785766 66-67 18.121008716729595 33.14454854798863 23.88244068428658 24.852002050995196 68-69 17.906586491399803 33.26807439518949 24.106185615065492 24.71915349834522 70-71 18.538199785577774 32.30550505756771 23.52584720085769 25.630447955996832 72-73 18.692024425488277 30.832517596606536 24.185428611383024 26.290029366522166 74-75 18.922761385354033 31.065585232834568 23.94303826970587 26.06861511210553 76 18.412343262014634 31.939588868689693 24.141145760499697 25.50692210879597 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 5.0 23 8.5 24 14.0 25 20.0 26 28.0 27 51.0 28 82.0 29 98.0 30 118.0 31 167.5 32 230.5 33 264.0 34 327.0 35 456.5 36 562.0 37 601.0 38 840.5 39 1304.0 40 1600.0 41 1657.5 42 1643.0 43 1606.0 44 1604.0 45 1649.5 46 1660.0 47 1648.5 48 1539.5 49 1337.5 50 1233.0 51 1117.0 52 898.5 53 674.5 54 553.0 55 567.0 56 464.5 57 278.5 58 209.0 59 178.5 60 134.5 61 103.0 62 85.0 63 72.5 64 43.0 65 23.0 66 18.0 67 16.0 68 17.0 69 13.0 70 5.5 71 3.0 72 3.5 73 2.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 21453.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.34480026103575 #Duplication Level Percentage of deduplicated Percentage of total 1 62.82342260553791 32.25656085395982 2 18.338629142078982 18.831865007225097 3 8.842487517022242 13.62047266116627 4 4.312301407172038 8.856570176665269 5 2.3876532001815707 6.1296788327972775 6 1.2346799818429415 3.803663823241505 7 0.6899682251475261 2.479839649466275 8 0.43576940535633224 1.7899594462312964 9 0.2451202905129369 1.1327087120682422 >10 0.6354970494779846 4.227846921176526 >50 0.027235587834770765 0.797091315899874 >100 0.018157058556513842 2.214142544166317 >500 0.009078529278256921 3.8596000559362325 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 828 3.8596000559362325 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 349 1.626812100871673 Illumina PCR Primer Index 10 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 126 0.5873304432946441 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 66 0.3076492798210041 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 53 0.24705169440171537 No Hit CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 52 0.24239034167715473 Illumina PCR Primer Index 10 (95% over 21bp) TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT 31 0.1445019344613807 Illumina PCR Primer Index 10 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATATCGTATGCCGT 30 0.13984058173682 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT 23 0.10721111266489534 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.009322705449121334 0.0 6 0.0 0.0 0.0 0.009322705449121334 0.0 7 0.0 0.0 0.0 0.013984058173682002 0.0 8 0.0 0.0 0.0 0.01864541089824267 0.0 9 0.0 0.0 0.0 0.03729082179648534 0.0 10 0.0 0.0 0.0 0.05593623269472801 0.0 11 0.0 0.0 0.0 0.05593623269472801 0.0 12 0.0 0.0 0.0 0.05593623269472801 0.0 13 0.0 0.0 0.0 0.06992029086841 0.0 14 0.0 0.0 0.0 0.07924299631753134 0.0 15 0.0 0.0 0.0 0.08390434904209201 0.0 16 0.0 0.0 0.0 0.10254975994033469 0.0 17 0.0 0.0 0.0 0.10254975994033469 0.0 18 0.0 0.0 0.0 0.11653381811401668 0.0 19 0.0 0.0 0.0 0.14450193446138068 0.0 20 0.0 0.0 0.0 0.14916328718594135 0.0 21 0.0 0.0 0.0 0.17713140353330536 0.0 22 0.0 0.0 0.0 0.20509951988066938 0.0 23 0.0 0.0 0.0 0.20976087260523005 0.0 24 0.0 0.0 0.0 0.20976087260523005 0.0 25 0.0 0.0 0.0 0.20976087260523005 0.0 26 0.0 0.0 0.0 0.22374493077891203 0.0 27 0.0 0.0 0.0 0.23772898895259403 0.0 28 0.0 0.0 0.0 0.25171304712627607 0.0 29 0.0 0.0 0.0 0.2563743998508367 0.0 30 0.0 0.0 0.0 0.2843425161982007 0.0 31 0.0 0.0 0.0 0.29366522164732206 0.0 32 0.0 0.0 0.0 0.3309560434438074 0.0 33 0.0 0.0 0.0 0.41019903976133876 0.0 34 0.0 0.0 0.0 0.4568125670069454 0.0 35 0.0 0.0 0.0 0.5873304432946441 0.0 36 0.0 0.0 0.0 0.7551391413788281 0.0 37 0.0 0.0 0.0 1.1886449447629701 0.0 38 0.0 0.0 0.0 1.556891810003263 0.0 39 0.0 0.0 0.0 2.0370111406330116 0.0 40 0.0 0.0 0.0 2.708245932969748 0.0 41 0.0 0.0 0.0 3.5239826597678645 0.0 42 0.0 0.0 0.0 4.311751270218617 0.0 43 0.0 0.0 0.0 5.010954178902717 0.0 44 0.0 0.0 0.0 5.840674963874516 0.0 45 0.0 0.0 0.0 6.320794294504265 0.0 46 0.0 0.0 0.0 7.085256141332215 0.0 47 0.0 0.0 0.0 7.868363399058406 0.0 48 0.0 0.0 0.0 8.847247471216146 0.0 49 0.0 0.0 0.0 9.476530089031836 0.0 50 0.0 0.0 0.0 10.091828648673845 0.0 51 0.0 0.0 0.0 10.860951848226355 0.0 52 0.0 0.0 0.0 11.504218524215727 0.0 53 0.0 0.0 0.0 12.026290029366523 0.0 54 0.0 0.0 0.0 12.730154290775182 0.0 55 0.0 0.0 0.0 13.154337388710204 0.0 56 0.0 0.0 0.0 13.75565189017853 0.0 57 0.0 0.0 0.0 14.482822915209994 0.0 58 0.0 0.0 0.0 15.088798769402882 0.0 59 0.0 0.0 0.0 15.722742739943131 0.0 60 0.0 0.0 0.0 16.244814245093927 0.0 61 0.0 0.0 0.0 16.83680604111313 0.0 62 0.0 0.0 0.0 17.447443248030577 0.0 63 0.0 0.0 0.0 17.974176105905933 0.0 64 0.0 0.0 0.0 18.50090896378129 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGACT 15 0.0021951767 70.0 8 ACCTGCC 20 0.0068598553 52.500004 36 TTGAAAA 195 0.0 35.897434 58 CTTGAAA 195 0.0 35.897434 57 GCTTGAA 210 0.0 35.0 56 CTGCTTG 215 0.0 34.186047 54 TGAAAAA 195 0.0 34.102566 59 TATGCCG 220 0.0 33.40909 43 GAAAAAA 220 0.0 33.40909 60 CCGTCTT 220 0.0 33.40909 47 ATGCCGT 220 0.0 33.40909 44 TCTTCTG 220 0.0 33.40909 50 AGATTAT 220 0.0 33.40909 32 CGTCTTC 220 0.0 33.40909 48 TGCCGTC 225 0.0 32.666668 45 TCTGCTT 225 0.0 32.666668 53 GTCTTCT 225 0.0 32.666668 49 GCCGTCT 225 0.0 32.666668 46 TGCTTGA 215 0.0 32.55814 55 CGTATGC 240 0.0 32.083332 41 >>END_MODULE