FastQCFastQC Report
Wed 25 May 2016
SRR1780649_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780649_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACGGTCACGTCCTGGCCGAGCGAGTACTCGGCGGCGTCGGCGGTGC11.1627906976744187No Hit
GTATATCAGACTGTCATAATTGATAAAGGAAAAAAATTAAACAGAATAAG11.1627906976744187No Hit
GGACCAGCTCGAGCTCGCTGGGGGCAGGCGGGGAGACCACACGACCACCC11.1627906976744187No Hit
TACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGA11.1627906976744187No Hit
CCTGCGCTCCGTGGTGGAGGAGATCCCGCCGGCCGCCTCGGGACGCCGGG11.1627906976744187No Hit
CCCGAGCACGTCACGGTGGCCGCCGTCCGCGCGCCCCTGCCCCTGCAGCT11.1627906976744187No Hit
CAGGAGGATAATGCCGATGTTTCAGGTTTCTGAGTAGAGAAATGATCCGT11.1627906976744187No Hit
AGCAGACTTTCCATATGAATAACAGGAGTGCCAGAAAAAGAAAAAATTAA11.1627906976744187No Hit
CCTAATACTAACTACCTGTCTCTTATACACGTCTGACGCTGCCGACGAAC11.1627906976744187No Hit
GTTGAGGTGGATTAAACCAAACCCAGCTACGCAAAATCTTAGCATCTGTC11.1627906976744187No Hit
GCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTTCTATA11.1627906976744187No Hit
GTGGAAGGTCGAGGACGGCGCCGAGGTGGTCGAGGGTGACACGGTCGCGA11.1627906976744187No Hit
CCCGAAGGCGGCGCAGTGGGCGCGGGCCGCGTCCAGGTAGGACCTGTCTC11.1627906976744187No Hit
TCGCCGGGTCGAGGGGGTCCAGGCCCACGCGGCCGGCTGTCTCTTATACA11.1627906976744187No Hit
TCTGGGGGATGTGGCTGCCCCGGCAGTGGGTGCGCTGTTTCTTATACACA11.1627906976744187No Hit
GGGCACGTTCACCAGAAAGGTCGCGCGCCAGCCGAGGTCGCCGCCGAGCC11.1627906976744187No Hit
GTGAGGAGCTAGACCACACTGAGGCGGTGGGCGGGGCTCGGGGTTTGCTC11.1627906976744187No Hit
ACGTGGGCTCTTCTGTCCCAGCCAGAAAGCGGCGGGGACGAGCTACCTGA11.1627906976744187No Hit
CTCTTATACACATCAGACGCCTCCGTGGAATGACGCAGTGTAAATCTCGA11.1627906976744187No Hit
GGATAACAGCTATCCATTGGTCTTAGGCCCCAAAAATTTTGGTGCAACTC11.1627906976744187No Hit
GAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTTAAGAGTACT11.1627906976744187No Hit
CTTACATACATCTTTCGCTGACGTCGAACCGGCACCTGCCAATTTACGAG11.1627906976744187No Hit
CATCTGACGCTGCCGACGAATGACCATGTGGGGGTCTTGGGGTTCGCCCG11.1627906976744187No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA11.1627906976744187No Hit
GCCATACTATTTATGTGCTCCGGATCCATCATCCACAACCTTAACAATGA11.1627906976744187No Hit
GTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGT11.1627906976744187No Hit
GGGCTATAGGGCCGCAGCCGCCCTCGTCCGGGGGGGCTTTTTCTATTTAG11.1627906976744187No Hit
GCCCAGCAGCGCGCCGGGGCCCGTCGGCGCGGTCGGTGGGCTGGCTCTTA11.1627906976744187No Hit
TTTGGGCGCAGGGGTAGGTAGCGTGAAGAGGCCAGCGAGAGGATGTAGAG11.1627906976744187No Hit
CGTTTACCACCATTGGACCAGTAGTATCCTTACTGTACTTCACAACAATC11.1627906976744187No Hit
AAGGTCGGGGCGGTGATGTAGAGGGGGATGGTAGATGTCTGTCTCTTATA11.1627906976744187No Hit
CGCTGCAACAAATCCTTTACAAAGCAGACTTGGACAACCACAAATCTGAC11.1627906976744187No Hit
TTGGGTCGGATTAGACGTCCGGGCATTGCATCCGTGGTTAAACCTAATGT11.1627906976744187No Hit
CTTATACACATCTGGCGGTGCCGGCGATGCAGGGGGGGTAGGTGTGGGTG11.1627906976744187No Hit
CCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAAC11.1627906976744187No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCGCCTTCGA11.1627906976744187No Hit
CTATTATTTTTGCACGTTCAGATGCAGCCACAGCTGTTTTTATTGATCTC11.1627906976744187No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGA11.1627906976744187No Hit
CTTATACACATCTGAGGGCGCCGACGAGATTCAATGGGGAGAGCTGGGCA11.1627906976744187No Hit
GCACAAACACTGTCTCTTATACACATCTGACGCTGCCGACGATAAACTGG11.1627906976744187No Hit
GCCGGAGCCCGGGCGCCTCGTCGCCGAGCGCATAGGCCTGTCTCTTATAC11.1627906976744187No Hit
CCGCACCTGCTACGGGCCGGGCCTGGCCGCCGGCGCTTAGATCCTGGGGG11.1627906976744187No Hit
CTCGCTCGCCGTGGCGGATGGCTCCCCGACCCAGGGCGTTTTGCTGCATA11.1627906976744187No Hit
GGTCGGAGGCTAGGTTCCAGTGCAGAGCATGGCTTAGCGACCACACTCAT11.1627906976744187No Hit
GAACTCGAATGAGGAAGCTCGCCCGGGAAGGAGACTGTCTCTTATACACA11.1627906976744187No Hit
GTCAAGGGCGGTCCGACGCGGTCGGCCGCCCCGGCCTCCCCAGGGTACCC11.1627906976744187No Hit
TCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATATCCCCTTTT11.1627906976744187No Hit
GTCAGGGGTGGAGACCTAATTGGGCTGATTTGCCTGCTGCTGCTAGGAGG11.1627906976744187No Hit
GTCTTAATCCATCTCTTATTCTCTGCTTAGCATAGTGTTCTGTACCTGTC11.1627906976744187No Hit
GAACACCTCTGATTACTCCTGCCATCATGACCCTTGGCCCTAATATGATT11.1627906976744187No Hit
GGCTTCTCCTTTTTTAACAAATCTCAAAATAAAAAGTCTTACAACCACAG11.1627906976744187No Hit
GCTTGTTAGGGACGGAACGGGGCACTGTGTAGGCGTTTTGGCTCCAACAT11.1627906976744187No Hit
AGCGCTTCTGCGGGAAATGGTGGTGGGTGGCAAGGCTAAACCTGAGTATT11.1627906976744187No Hit
GCGTCAGATGTGTATAAGAGACAGAGCCTGTCACTTCTCTTGACTCTAGG11.1627906976744187No Hit
CTTATACACATCTGACGCCGCCGACGATAAACGGGGTGTAGATCTCGGGG11.1627906976744187No Hit
GGACTGGGAGCGGATGCAGGCCGCCCACCCGGACCTGTCTCTTATACACA11.1627906976744187No Hit
CACCAGTCTCACCCCGTGAAGGCCGCGGGCGACCAGATCAGCGAAGAACC11.1627906976744187No Hit
GGTCTCCACCCCTGACTCCCCTCAGCCATAGAAGGCCCCACCCCAGTCTC11.1627906976744187No Hit
AGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTG11.1627906976744187No Hit
GTATGGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTACTGTC11.1627906976744187No Hit
ACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGTTAAATA11.1627906976744187No Hit
ACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAAGCTATCGGGC11.1627906976744187No Hit
CATAAATATGATGCTCGCATTTACCATCTCCCTTCTGGGAATACTAGGAT11.1627906976744187No Hit
GCTCATGGGTGAACTCGCGGATCGCCTTGGCGACGAGCAGCCGGTGGGCT11.1627906976744187No Hit
CCTCAGGCTAGCTACCTGCCTCCGCCCCCTCACAATCCTGGCCCCGCCCC11.1627906976744187No Hit
CACACACGCCAGAGGGAAACTTGAGCTTCAGTATGATTTAGTAACATAGA11.1627906976744187No Hit
GCCAGGCGATCTACCTGCTGGGCGAGACCGCGGACGAGCTGGACCTGTCT11.1627906976744187No Hit
GCGCATGGGCGACCTCGCCCGCCACGTGGCCCAGCCTGTCTCTTAACACA11.1627906976744187No Hit
GTGTTATTCTTCTGCATTTTGGGGGAGGTTATATCGGTTCCCTCGTCCTT11.1627906976744187No Hit
TCCTCCGGCACGGGGGCCAGGAAGCCGGGCTCGGCGAGCTCGGGGACGAA11.1627906976744187No Hit
CTCCCTGTCCAACCCCGCCCACGCGCCCCTGGCCCCGGAATCTGTCTCTT11.1627906976744187No Hit
GACCTAGACTAGTGGACTCTATACTTTTTGACTCCACAAACCATTTACAA11.1627906976744187No Hit
CTATCCTAGAAATCGCTGTCGCCTTAATCCAAGCCTACGTTTTCACCTGT11.1627906976744187No Hit
GCACAAGAATCACAGATTAATGTGGTGGCCTGGAGATAGGGACAGGTCAC11.1627906976744187No Hit
CACTTCCACTCCATAACGCTCTTCGTACTAGGCCTACTAACCAACACACT11.1627906976744187No Hit
CCGTGATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAATAT11.1627906976744187No Hit
GGCAGGATCCGCGCCGATGTCGTGCACGCCAAGCCTGTCTCTTATACACA11.1627906976744187No Hit
CTTCACCAAAGATGCCCTAAAACCCGCAACATCTACCATCACCCTCTACA11.1627906976744187No Hit
AGCCTGCTTGGCGGCCCAGCGCTCGGCCGCGGCCTGGTCCTGTCTCTTAT11.1627906976744187No Hit
CCGCTCCAACTCAGAAGTCAACGGCGGTACTCGGGTCGCTGCGTTGGGGC11.1627906976744187No Hit
GGCCGACCTCGTGCAGGTCGCGGGCCGGATCGACGGCTGTCTCTTATACA11.1627906976744187No Hit
ACACGAAAGTGGACGAATAGGGTGTGACGCCTGCCCCTGTCTCTTATACA11.1627906976744187No Hit
TCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTACTA11.1627906976744187No Hit
GGCGCCGTCGGACCGGGACAGCCGTGCCTTGCTCGCACTCCTCCTCTACC11.1627906976744187No Hit
GTTAGTAGTATGGTGATGCCAGCAGCTAGGACCGGGAGAGATAGGAGACT11.1627906976744187No Hit
AATTAGTACGGGAAGGGTATAACCAACATTTTCGGGGTATGGGCCCGATA11.1627906976744187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCC50.044.020
CCGGATC50.044.025
GTGCAGG50.044.011
TCGACGG50.044.030
GTCGCGG50.044.017
TGTCTCT50.044.038
CCTCGTG50.044.07
GCAGGTC50.044.013
GGCCGGA50.044.023
GGCCGAC50.044.01
TCGCGGG50.044.018
CTCTTAT50.044.041
GACGGCT50.044.032
GGATCGA50.044.027
GGCTGTC50.044.035
GATCGAC50.044.028
CGGATCG50.044.026
TCGTGCA50.044.09
TTATACA50.044.044
CGGGCCG50.044.021
Produced by FastQC (version 0.11.5)