FastQCFastQC Report
Wed 25 May 2016
SRR1780649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG44.651162790697675Illumina PCR Primer Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCAGAGACACCGGCTAATCTCGTATGCCGTC22.3255813953488373No Hit
GTACAGATGTGCAGGAATGCTAGGTGTGGTTGGTTGATGCCGATTGTAAC11.1627906976744187No Hit
ATTTCTAGGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTC11.1627906976744187No Hit
TCCTTATACACATCTCCGAGCCCAGAGACACCGGCTAATCTCGTATGCCG11.1627906976744187No Hit
CCCTTACTAACATTAACGAAAATAACCCCACCCTACTAAACCCCATTAAA11.1627906976744187No Hit
CGCTCCCACTGCCGCGGCAGCCACATCCCCCAGACTGTCTCTTATACACA11.1627906976744187No Hit
ATGCTAAGATTTTGCGTAGCTGGGTTTGGTTTAATCCACCTCAACCTGTC11.1627906976744187No Hit
TACTTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACCATACCTGTC11.1627906976744187No Hit
GTTCCTAGGCAGATTGTAGTGGTGAGGGCGCTTCTTATAATAATGCTTGT11.1627906976744187No Hit
CGTCCGGGCGTCGGGTGGCCATCCCGCGGGGGATGGCGAGCAGGGCGTGC11.1627906976744187No Hit
CCATCAGAGAAGCATCGTACAAGTAAATCACAAGTCCCTATCACAGGGAC11.1627906976744187No Hit
ACTCCCACCATTGCCACAGGCATTCCTAAGACTCCTTATTCTGTTTAATT11.1627906976744187No Hit
CTCACCACTCCTATTCAACGTAGTGTTGGAAGTTCTGGCCAGGGCAATTA11.1627906976744187No Hit
GCCTTCGGCACCGGCATCGGCGGCGTGTGGACCTGTCTCTTATACACATC11.1627906976744187No Hit
GCCTCGACGATCGCGACCGTGTCACCCTCGACCACCTCGGCGCCGTCCTC11.1627906976744187No Hit
TCTCCTTCCCGGGCGAGCTTCCTCATTCGAGTTCCTGTCTCTTATACACA11.1627906976744187No Hit
TCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCTTG11.1627906976744187RNA PCR Primer, Index 14 (96% over 28bp)
CCACTTATGACTCCCTAAAGCCCATGTCGAAGCCCCCATCGCTGGGTCAA11.1627906976744187No Hit
CAGCAGGGCCACGGTCGAGGAGACGACCGCCACGGCGAGCGAGCTGTCTC11.1627906976744187No Hit
GAATCATCCGCTACCTTCACGCCAATGGCGCCTCAATATTCTTTATCTGC11.1627906976744187No Hit
CCGGAGGGGTACCCTGGGGAGGCCGGGGCGGCCGACCGCGTCGGACCGCC11.1627906976744187No Hit
GAGCAAACCCCGAGTCCCGACCACCGCCTCAGTGTGGTCTAGCTCCTCAC11.1627906976744187No Hit
GGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTCTGTCTCTTATACA11.1627906976744187No Hit
GTCCGGGTGGGCGGCCTGCATCCGCTCCCAGTCCCTGTCTCTTATACACA11.1627906976744187No Hit
CAGTTGGCCAGTATTGAGTGCCTGGGTGCTGTGTTGAAAAGGACCTGTCT11.1627906976744187No Hit
ATTTGGAGTTGCACCAAAATTTTTGGGGCCTAAGACCAATGGATAGCTGT11.1627906976744187No Hit
CTCGTCGAGCTGCGCACCGCCGACGCCGCCGAGTACTCGCTCGGCCAGGA11.1627906976744187No Hit
TGTTTGTGCCTGTCTCTTATACACATCTCCGAGCCCATGAGACGATCCAA11.1627906976744187No Hit
TTTCACAGTAGAATAATATGTTTTTTATCTATCTTGCCTGGAATTTAGTG11.1627906976744187No Hit
CCGCACCGGCGCGCCCGCCCCGCGTCCCGAGGCGGCCGGCGGGATCTCCT11.1627906976744187No Hit
CTCTTATACACATCTCAGAGCCCACGAGACACCGGCTAATCTCGTATGCC11.1627906976744187No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT11.1627906976744187No Hit
ATTCCGGGGCCAGGGGCGCGTGGGCGGGGTTGGACAGGGAGCTGTCTCTT11.1627906976744187No Hit
ATACACATCTCCGAGCCCAGAGACCTCATGGGATCTCGTATGCCGTCTTC11.1627906976744187Illumina PCR Primer Index 3 (95% over 21bp)
CACCACACACACCACACACATCACATACGACACCCACTACGTGCATACCA11.1627906976744187No Hit
TCTTATACACATCTCCGAGCCCAGAGACGGCGTCGAATCTCGTATGCCGT11.1627906976744187No Hit
CTCATGAGACCCACAACAAATAGCCCTTCTAAACGCTAATCCAAGCCTCA11.1627906976744187No Hit
CTACGATGTTAACAGTAGTGTGACTGATTTTGTTCAATTTCTTTGTAACT11.1627906976744187No Hit
GTTCTTCGCTGATCTGGTCGCCCGCGGCCTTCACGGGGTGAGACTGGTGC11.1627906976744187No Hit
GCCTCCTCCTAGCAGCAGCAGGCAAATCAGCCCAATTAGGTCTCCACCCC11.1627906976744187No Hit
ATACACATCTCCAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTC11.1627906976744187TruSeq Adapter, Index 10 (95% over 22bp)
GAATAAATAGGAGGCCTAGGTTGAGGTTGACCAGGGGGTTGGGTATGGGG11.1627906976744187No Hit
CCCACCGACCGCGCCGACGGGCCCCGGCGCGCTGCTGGGCCTGTCTCTTA11.1627906976744187No Hit
GCTTGGCGTGCACGACATCGGCGCGGATCCTGCCCTGTCTCTTATACACA11.1627906976744187No Hit
GTCCTACCTGGACGCGGCCCGCGCCCACTGCGCCGCCTTCGGGCTGTCTC11.1627906976744187No Hit
CCCACGAGACATGATGATATCTCGTATGCCGTCTTTTGCTTGAAAAAAAA11.1627906976744187TruSeq Adapter, Index 25 (96% over 28bp)
AAATGATAATGCCAGCAATTCAAGGACTCTGACAAACATCCCAACAAATC11.1627906976744187No Hit
GTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGCTGT11.1627906976744187No Hit
TAGATGCAGCGCTCCTGACCCCGGGTCAGGACCAGCCCCTCCGCCAGCTC11.1627906976744187No Hit
ATATAAACATCTCCGAGCCCAGAGACTTTAATGCATCTCGTATGCCGTCT11.1627906976744187No Hit
GTAGTTAGTATTAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG11.1627906976744187No Hit
AATCTTAGTTACCGCTAATAACCTATTCCAACTGTTCATCGGCTGAGAGG11.1627906976744187No Hit
GACCAGGCCGCGGCCGAGCGCTGGGCCGCCAAGCAGGCTCTGTCTCTTAT11.1627906976744187No Hit
GCAAAGCAGGACCAAAGAGGGTGAAGGTGAAAAGTACGATTTCCTTAATT11.1627906976744187No Hit
CCGGCCGCGTGGGCCTGGACCCCCTCGACCCGGCGACTGTCTCTTATACA11.1627906976744187No Hit
GGACATCATGTCCCTCGACCCGCCGTTCGTCCCCGAGCTCGCCGAGCCCG11.1627906976744187No Hit
AGCCCACGAGACCGAGCCCACGGACCCAACACGATATCGTATGCCGTCTT11.1627906976744187TruSeq Adapter, Index 1 (95% over 21bp)
TCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGG11.1627906976744187No Hit
CCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCA11.1627906976744187No Hit
CCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAA11.1627906976744187No Hit
ATCTGCCTCTTCCTACACATCGGGCGAGGCCTATATTACGGATCATTTCT11.1627906976744187No Hit
TCGCGTGGCTCGGCGGCGACCTCGGCTGGCGCGCGACCTTTCTGGTGAAC11.1627906976744187No Hit
ACATCTACCATCACCCTCTACATCACCGCCCCGACCTTCTGTCTCTTATA11.1627906976744187No Hit
CTCTTATACACATCTCCGAGCCCACGAGCTTTAATGCATCTCGTATGCCG11.1627906976744187No Hit
ATCTCCGAGCCGACGAGACCCAACACGATCTCGTATGCCGTCTTCTGCTT11.1627906976744187TruSeq Adapter, Index 1 (96% over 30bp)
CTTATACACATCTCCGAAGCCCACGAGACTTTAATGCATCTCGTATGCCG11.1627906976744187No Hit
TCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACCATACTACTAACCT11.1627906976744187No Hit
CACCAGTCTTGTAAACCGAAGATGAAAACCTTTTTCCAAGGACAAATCAG11.1627906976744187No Hit
GCTGGGCCACGTGGCGGGCGAGGTCCCCCCCGCGCCTGTCTCTTATACAC11.1627906976744187No Hit
GTATCCGCCCGCCCCCTCTCCCCCCGGGCACTTTCGAACGTGGGGTAGGG11.1627906976744187No Hit
AACCTGCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGGGCATGCGT11.1627906976744187No Hit
CCCCAGAAATTCTTCAGCAAGCAAAACAGTGTACAATAGGAAAAAAAATA11.1627906976744187No Hit
CCCACCGGCTGCTCGTCGCCAAGGCGATCCGCGAGTTCACCCATGAGCCT11.1627906976744187No Hit
GCCTATGCGCTCGGCGACGAGGCGCCCGGGCTCCGGCCTGTCTCTTATAC11.1627906976744187No Hit
TCTGTGGAACGAGGGTTTATTTTTTTGGTTAGAACTGGAATAAAAGCTAG11.1627906976744187No Hit
CTTATACACATCTCCGAGCCCAGAGACTTTAATGCATCTCGTATGCCGTC11.1627906976744187No Hit
CCGTCGATCCGGCCCGCGACCTGCACGAGGTCGGCCCTGTCTCTTATACA11.1627906976744187No Hit
GTTTACACATCTCCGAGCCCACGAGACCGCGATCAATCTCGTAAGCGGCC11.1627906976744187No Hit
GGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACT11.1627906976744187No Hit
CTGCAGGGGCAGGGGCGCGCGGACGGCGGCCACCGTGACGTGCTCGGGCT11.1627906976744187No Hit
GTCCAGCTCGTCCGCGGTCTCGCCCAGCAGGTAGATCGCCTGGCCTGTCT11.1627906976744187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAC50.044.014
CGCGACC50.044.015
GAGGTCG50.044.027
TGCACGA50.044.022
TCGATCC50.044.04
GCGACCT50.044.016
TGTCTCT50.044.038
CCTGTCT50.044.036
CCTGCAC50.044.020
TCGGCCC50.044.031
CTCTTAT50.044.041
GTCGATC50.044.03
CACGAGG50.044.024
CCGTCGA50.044.01
CGAGGTC50.044.026
TTATACA50.044.044
ACCTGCA50.044.019
CCCGCGA50.044.013
GGCCCTG50.044.033
GGCCCGC50.044.011