FastQCFastQC Report
Wed 25 May 2016
SRR1780648_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780648_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105149
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGT200.0069373652.49464859
GTTCTAC200.0069373652.49464833
ATACTGG200.0069373652.4946486
CTGGGTA200.0069373652.49464831
GATTCAA200.0069373652.4946489
AGTGCGC350.001251198139.9959228
TCATGTC350.001251198139.99592236
AACTTCC400.002410684734.99643322
AGAAAAG400.002410684734.99643320
TCCACGA400.002410684734.99643312
GGGTTCA400.002410684734.99643319
TTTGAGG400.002410684734.99643335
GACTGTT400.002410684734.9964336
TGTGAAA552.9768972E-431.81493810
TGCTTGT450.00429298731.10793731
ACCAGTG450.00429298731.10793743
GTGTATT450.00429298731.10793726
CTTTGAG450.00429298731.10793734
CCTATTG450.00429298731.1079372
CTAGACC500.0071846427.9971453
Produced by FastQC (version 0.11.5)