##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780648_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 105149 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.260059534565237 34.0 31.0 34.0 12.0 34.0 2 31.318747681860977 34.0 32.0 34.0 23.0 34.0 3 31.516153268219384 34.0 33.0 34.0 23.0 34.0 4 31.960265908377636 34.0 34.0 34.0 27.0 34.0 5 31.928025944136415 34.0 34.0 34.0 27.0 34.0 6 34.378053999562525 38.0 35.0 38.0 24.0 38.0 7 33.76439148256284 37.0 34.0 38.0 22.0 38.0 8 32.36679378786294 37.0 31.0 38.0 11.0 38.0 9 33.34959914026762 37.0 34.0 38.0 21.0 38.0 10-11 33.87725513319195 37.0 34.0 38.0 22.5 38.0 12-13 34.31096824506177 38.0 34.5 38.0 24.5 38.0 14-15 33.91070290730297 37.0 34.0 38.0 22.5 38.0 16-17 33.80834815357255 37.0 34.0 38.0 22.0 38.0 18-19 33.758485577608916 37.0 34.0 38.0 22.0 38.0 20-21 33.8420859922586 37.0 34.0 38.0 22.5 38.0 22-23 33.886213848919155 37.0 34.0 38.0 22.0 38.0 24-25 34.34406413755718 38.0 34.5 38.0 24.0 38.0 26-27 34.43813540784981 38.0 35.0 38.0 25.0 38.0 28-29 34.561351035197674 38.0 35.5 38.0 25.5 38.0 30-31 34.39648974312642 38.0 35.0 38.0 24.0 38.0 32-33 34.13301600585835 38.0 34.0 38.0 23.5 38.0 34-35 33.95002805542611 37.0 34.0 38.0 23.0 38.0 36-37 33.95328058279203 37.0 34.0 38.0 23.5 38.0 38-39 34.550894445025634 38.0 35.0 38.0 25.5 38.0 40-41 33.997636686987036 37.5 34.0 38.0 23.0 38.0 42-43 34.35583790620929 37.5 34.5 38.0 24.5 38.0 44-45 33.32144861101865 37.0 34.0 38.0 21.5 38.0 46-47 34.067128550913466 37.0 34.0 38.0 24.0 38.0 48-49 33.810549791248604 37.0 34.0 38.0 23.0 38.0 50-51 33.74875177129597 37.0 34.0 38.0 22.5 38.0 52-53 33.774001654794624 37.0 34.0 38.0 23.0 38.0 54-55 33.4952115569335 37.0 34.0 38.0 21.5 38.0 56-57 33.585274229902325 37.0 34.0 38.0 22.0 38.0 58-59 33.473770554165995 37.0 33.5 38.0 22.5 38.0 60-61 33.502006676240384 37.0 33.5 38.0 22.0 38.0 62-63 33.57390940474945 37.0 33.5 38.0 22.5 38.0 64-65 33.600390873902754 37.0 34.0 38.0 22.5 38.0 66-67 33.52792703687149 37.0 33.5 38.0 22.5 38.0 68-69 33.127533309874565 37.0 32.5 38.0 20.5 38.0 70-71 32.89738846779332 37.0 32.0 38.0 20.0 38.0 72-73 33.01270102426081 37.0 32.0 38.0 20.5 38.0 74-75 32.5643087428316 37.0 31.0 38.0 19.5 38.0 76 31.82090176796736 36.0 30.0 37.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.851641624872176 1101 2 -0.3805427352771211 1101 3 -0.6377630787272395 1101 4 -2.1825189344734213 1101 5 -1.1000644416817842 1101 6 -2.73688124226549 1101 7 -1.5118253169021614 1101 8 -0.5568786119296689 1101 9 -1.0283819386063797 1101 10-11 -1.0048707701221034 1101 12-13 -1.3000714740785924 1101 14-15 -0.4340458252405597 1101 16-17 -1.6063717349162232 1101 18-19 -0.28733842808317434 1101 20-21 -1.071613918824312 1101 22-23 -0.03314412847775827 1101 24-25 -1.2374015334997068 1101 26-27 -1.0289764243833162 1101 28-29 -0.05350652758715313 1101 30-31 -0.9841763166271775 1101 32-33 -0.09445376295155938 1101 34-35 -1.0553318773136624 1101 36-37 0.09779168218359047 1101 38-39 -0.5942455850268971 1101 40-41 0.04542507675485297 1101 42-43 -0.32895812627144494 1101 44-45 0.04739668817781251 1101 46-47 0.45813637225658255 1101 48-49 -0.7779028827899879 1101 50-51 -1.0136270667674196 1101 52-53 -0.3635105746932368 1101 54-55 -1.6102858872249968 1101 56-57 -0.8079942456083558 1101 58-59 -0.09024424629055972 1101 60-61 0.1723208670555607 1101 62-63 -1.9385854781072247 1101 64-65 -0.6707627174024253 1101 66-67 -1.1108863321630338 1101 68-69 0.06737350546789855 1101 70-71 0.36555905432002334 1101 72-73 -0.12318387272598841 1101 74-75 -1.2050860561412335 1101 76 -1.5352025273358585 1102 1 0.708601403636937 1102 2 -0.3415802800660259 1102 3 0.10258588528584767 1102 4 0.2687039974474388 1102 5 0.41747723147602755 1102 6 0.797454324022091 1102 7 1.5775655539500022 1102 8 2.458061409880578 1102 9 0.6428129515634282 1102 10-11 -0.7048084551197675 1102 12-13 -4.315572330909877 1102 14-15 -1.586982419725679 1102 16-17 -2.360835046958602 1102 18-19 -0.7764410920495237 1102 20-21 0.09962770759725004 1102 22-23 -1.3775903038944932 1102 24-25 0.06137553457942602 1102 26-27 -6.295731370636624 1102 28-29 -0.3886677676556971 1102 30-31 0.5724602311216884 1102 32-33 -6.487077224549942 1102 34-35 0.14157574069537304 1102 36-37 0.5416848119545818 1102 38-39 0.745028445195878 1102 40-41 0.33619466703370904 1102 42-43 0.40405324621647765 1102 44-45 -6.095974553448606 1102 46-47 -0.4387322988759905 1102 48-49 0.769144943974311 1102 50-51 -6.244659937931154 1102 52-53 -1.5363022867979197 1102 54-55 0.9862945770217735 1102 56-57 -0.8859035772799544 1102 58-59 1.192089450546952 1102 60-61 0.4396911739569447 1102 62-63 -4.251632681721496 1102 64-65 -0.1844953860423999 1102 66-67 0.7363017033565171 1102 68-69 -7.399802067051187 1102 70-71 -0.22173647457855594 1102 72-73 0.3438359122872612 1102 74-75 -6.119293876674032 1102 76 -1.9800537502677784 1103 1 -0.11170189058920243 1103 2 -0.45219045476759945 1103 3 0.499916691167293 1103 4 0.6662141157936645 1103 5 0.8461621026272184 1103 6 -1.0268963086947451 1103 7 1.0107332367206325 1103 8 0.9870331809754092 1103 9 0.6988609048397194 1103 10-11 1.097806945259343 1103 12-13 -1.5211028396449535 1103 14-15 -0.958261686081542 1103 16-17 -0.8987084011263349 1103 18-19 -0.03421556820097038 1103 20-21 -0.32164131233205495 1103 22-23 -0.3551890538304505 1103 24-25 0.9725902484479718 1103 26-27 -0.30259647643098475 1103 28-29 0.9331596825210511 1103 30-31 0.38237621042390657 1103 32-33 -4.627017795275901 1103 34-35 -1.1632554494270693 1103 36-37 0.3249728902372766 1103 38-39 0.8068912455442572 1103 40-41 1.238008284534601 1103 42-43 0.9045783755776142 1103 44-45 -4.69951469382465 1103 46-47 0.44680230842971014 1103 48-49 0.14645679396662103 1103 50-51 0.5964948343420176 1103 52-53 0.0031720715196144056 1103 54-55 0.532749313432447 1103 56-57 1.3769941121508609 1103 58-59 0.6179053222616915 1103 60-61 1.2673078551393786 1103 62-63 1.0432594746389725 1103 64-65 1.2698823997048194 1103 66-67 0.30246800285956255 1103 68-69 -2.1393379039280767 1103 70-71 1.031234989123483 1103 72-73 1.695121838820377 1103 74-75 -1.273323283918252 1103 76 0.6349111557212552 1104 1 -2.155441765618132 1104 2 -0.295884044214489 1104 3 0.0021383991052736917 1104 4 -0.22432048267890892 1104 5 -0.21653171824758388 1104 6 -0.49127955331404394 1104 7 1.7912007210992513 1104 8 2.180067200878213 1104 9 0.092589208473143 1104 10-11 0.03168799131350397 1104 12-13 -0.26259786394488316 1104 14-15 0.2101484041753494 1104 16-17 -0.1725223330865333 1104 18-19 1.0073835986585493 1104 20-21 1.4815176786422626 1104 22-23 0.8564054844708693 1104 24-25 0.6147448422919837 1104 26-27 0.5363296980656642 1104 28-29 -0.1873911159052355 1104 30-31 -1.0277371892520932 1104 32-33 -0.23512934352861947 1104 34-35 0.6552240692028732 1104 36-37 0.8046882339077257 1104 38-39 0.5507931201103347 1104 40-41 0.8316671597949608 1104 42-43 0.7255457442422824 1104 44-45 -2.918190926178273 1104 46-47 0.7697830999131625 1104 48-49 1.0515482079911536 1104 50-51 1.3387304279546015 1104 52-53 0.25003622333632336 1104 54-55 0.34928483761666484 1104 56-57 1.1167418557287334 1104 58-59 1.1720710246454047 1104 60-61 0.8143687206797665 1104 62-63 1.213173846312202 1104 64-65 0.29091919674253575 1104 66-67 1.0581495580551277 1104 68-69 -3.2205575290148616 1104 70-71 0.2135886109702696 1104 72-73 1.313867499547058 1104 74-75 0.019861164126176334 1104 76 1.1727496190399336 1105 1 -0.4439166556898755 1105 2 0.22230618952769987 1105 3 -0.4665262618528381 1105 4 -0.09553085064928624 1105 5 0.16830975363392753 1105 6 0.5033050335871323 1105 7 1.2959681360494955 1105 8 1.4870331809754092 1105 9 0.9337602336987842 1105 10-11 0.943444529151968 1105 12-13 -1.876807537631528 1105 14-15 -0.2502079948063738 1105 16-17 0.5375332096119223 1105 18-19 0.4020260425372868 1105 20-21 -0.7360708425333939 1105 22-23 -0.07498771154857309 1105 24-25 0.33836206052851026 1105 26-27 0.041363255112642605 1105 28-29 -1.1440215255326365 1105 30-31 -0.23339560165663187 1105 32-33 0.5256667684824876 1105 34-35 0.6001673693648755 1105 36-37 0.529670876814464 1105 38-39 0.6374281582959398 1105 40-41 0.8688807677560817 1105 42-43 0.9079340802756022 1105 44-45 1.7820289303364234 1105 46-47 1.6582117044028593 1105 48-49 0.40149035101360653 1105 50-51 0.42032033769772426 1105 52-53 -0.4129353110307221 1105 54-55 -0.6836936395876876 1105 56-57 0.24947733362736813 1105 58-59 0.226965724946254 1105 60-61 1.0491870497702536 1105 62-63 0.6539977296725326 1105 64-65 -0.22340619089921177 1105 66-67 0.4400518954770192 1105 68-69 1.6744204853336697 1105 70-71 1.702375928720798 1105 72-73 0.7068668052633313 1105 74-75 -1.0031890557303313 1105 76 -0.7476391798492124 1106 1 -0.6380717192980079 1106 2 0.06826932686117004 1106 3 -0.6739038036739338 1106 4 -0.25713057017247465 1106 5 0.4639623630699674 1106 6 -0.27882458676747035 1106 7 1.3120705100853556 1106 8 1.4764902580063648 1106 9 1.420612883202942 1106 10-11 1.0412608168410387 1106 12-13 0.20697238824245545 1106 14-15 0.34172328837503585 1106 16-17 0.49626305034115603 1106 18-19 -0.18864059073928274 1106 20-21 -0.35499801873293535 1106 22-23 0.22637460438851775 1106 24-25 0.829747415632184 1106 26-27 0.4318520861253674 1106 28-29 -0.8088141730333902 1106 30-31 0.08853918748490486 1106 32-33 0.882510903512042 1106 34-35 0.7592127465060159 1106 36-37 0.5630901896462746 1106 38-39 -0.02522334926692338 1106 40-41 0.4585456981377334 1106 42-43 0.5978694704388801 1106 44-45 1.0390658931732517 1106 46-47 0.501808318647079 1106 48-49 0.3424900505027324 1106 50-51 0.19605158081100882 1106 52-53 -0.30945940198535027 1106 54-55 0.25329924832175976 1106 56-57 1.0620712432737633 1106 58-59 -0.3222804936253141 1106 60-61 1.0436275987034378 1106 62-63 0.7849829044696861 1106 64-65 0.8449850742515537 1106 66-67 1.353041477766908 1106 68-69 0.9550600726320724 1106 70-71 0.2558042568786618 1106 72-73 0.060367756881078094 1106 74-75 -1.5309237046334694 1106 76 -1.5893450798792657 1107 1 -0.2561978659732702 1107 2 -0.0028493323345486488 1107 3 0.1901438980169452 1107 4 0.46636573567371187 1107 5 0.4190622787738434 1107 6 0.9124780363296665 1107 7 0.6174580243413175 1107 8 0.3919585973726569 1107 9 -0.5351688394132168 1107 10-11 0.13290806234110875 1107 12-13 0.9104173726228808 1107 14-15 0.1382845022392658 1107 16-17 1.001506765321075 1107 18-19 1.4443179554004502 1107 20-21 1.2293352980717458 1107 22-23 -0.44534156559213756 1107 24-25 -0.2650237451750783 1107 26-27 0.12769182431151194 1107 28-29 -0.7230366652236029 1107 30-31 0.28677764635335734 1107 32-33 1.6817683538021129 1107 34-35 0.5640796082107826 1107 36-37 -0.0520422048596032 1107 38-39 0.8886210499992089 1107 40-41 0.8333225613300783 1107 42-43 0.6494946501879753 1107 44-45 0.16229939344597 1107 46-47 0.6183167234668332 1107 48-49 1.0142186169727125 1107 50-51 1.7552386190417835 1107 52-53 1.3172203223167287 1107 54-55 0.7832119770369914 1107 56-57 0.7507148194130053 1107 58-59 1.0665663849386746 1107 60-61 -0.4529757632244582 1107 62-63 -0.15102021944561983 1107 64-65 0.5417669456255823 1107 66-67 1.3080980057581755 1107 68-69 1.5384698509828496 1107 70-71 0.349279984552588 1107 72-73 0.6617710394714464 1107 74-75 0.35476206915539166 1107 76 -0.24379294024520703 1108 1 -0.5252896699487941 1108 2 -0.30769177671187364 1108 3 -0.8802835212476836 1108 4 -0.3246339623027339 1108 5 -0.14760103207551722 1108 6 0.11591886767747894 1108 7 0.8080125172213926 1108 8 0.24081258576152464 1108 9 -0.5363321894137769 1108 10-11 -0.08071767454044476 1108 12-13 0.11819280132854004 1108 14-15 0.5248355050693405 1108 16-17 -0.905402862518784 1108 18-19 -0.18801870019201772 1108 20-21 0.3973890358929282 1108 22-23 -0.7119388787010834 1108 24-25 -1.2232326336165187 1108 26-27 -1.0276433659074016 1108 28-29 0.38772208507003114 1108 30-31 0.6542899789819714 1108 32-33 0.5817985561579064 1108 34-35 0.05006907094443136 1108 36-37 0.27725296164486934 1108 38-39 0.6771486292244546 1108 40-41 -0.09273200425937489 1108 42-43 0.4273395443119412 1108 44-45 0.0839802104756231 1108 46-47 0.6520482804542027 1108 48-49 -0.05915073546640315 1108 50-51 0.33860271403379016 1108 52-53 0.5109963998137346 1108 54-55 0.5227725886906214 1108 56-57 0.09586062967509434 1108 58-59 -1.2334380894844017 1108 60-61 0.9365619170108914 1108 62-63 0.2003618179829303 1108 64-65 -0.05541193062306604 1108 66-67 -0.06699847398946446 1108 68-69 1.5441411822355775 1108 70-71 1.5074060961670668 1108 72-73 0.4415175634040196 1108 74-75 0.7008027640332983 1108 76 0.5490412711936514 1109 1 -0.00946475412387926 1109 2 -0.0982978373179364 1109 3 0.29937977841561647 1109 4 0.34809331042453806 1109 5 0.5504797760052895 1109 6 1.023662975421587 1109 7 -0.24454640533753036 1109 8 1.1745499594989042 1109 9 1.541209898128315 1109 10-11 1.531285692462923 1109 12-13 0.6614251245608642 1109 14-15 0.5052058754397066 1109 16-17 -0.7021200342359606 1109 18-19 0.4919812998079749 1109 20-21 1.2680119315158223 1109 22-23 1.2578422660800612 1109 24-25 1.0203195549356678 1109 26-27 0.9979963647323302 1109 28-29 0.6747927921407424 1109 30-31 1.0773202820122734 1109 32-33 1.4278591622185104 1109 34-35 0.38602866690402493 1109 36-37 0.5627916821835868 1109 38-39 0.565448292524124 1109 40-41 0.6245407230133537 1109 42-43 0.6095112614836609 1109 44-45 0.10249872899414214 1109 46-47 0.19041528382120987 1109 48-49 0.14689303557736366 1109 50-51 1.2921212325523044 1109 52-53 1.461013234830574 1109 54-55 0.767503228421262 1109 56-57 0.6903050741195358 1109 58-59 0.1518986108522995 1109 60-61 1.0638514793004532 1109 62-63 0.46886350148460565 1109 64-65 0.060631840420704464 1109 66-67 0.7340116270206423 1109 68-69 1.6557068388012226 1109 70-71 0.5684161971771715 1109 72-73 -0.03774169585523168 1109 74-75 2.3245738078043416 1109 76 0.28826686041924177 1110 1 0.3972692526101298 1110 2 0.13675266773256922 1110 3 -0.028431669395828862 1110 4 0.02216738449861566 1110 5 -0.251978136452621 1110 6 -0.31842456666595353 1110 7 0.5891572983661746 1110 8 2.139802484751428 1110 9 -0.9673086203902059 1110 10-11 -0.9376200314427976 1110 12-13 0.05085273398847079 1110 14-15 -0.29502981479597423 1110 16-17 -0.00472946184538614 1110 18-19 0.7059206937473732 1110 20-21 -0.08072544222154932 1110 22-23 0.21567054890834925 1110 24-25 -0.1643100746939652 1110 26-27 -1.4889228271868618 1110 28-29 0.08300828035622487 1110 30-31 -0.45008712539513596 1110 32-33 0.7501487245080654 1110 34-35 0.5248165456919054 1110 36-37 -0.4247672403753313 1110 38-39 0.3269802790561087 1110 40-41 -1.394116667796652E-4 1110 42-43 0.3296964466688479 1110 44-45 1.477919604415014 1110 46-47 0.4348765632824865 1110 48-49 0.6849570086413337 1110 50-51 0.41772729315836443 1110 52-53 0.2113331001504406 1110 54-55 8.870668050988684E-4 1110 56-57 0.3685879024023677 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0.9812657293042975 2116 50-51 0.8583512448524289 2116 52-53 1.3822985976841977 2116 54-55 0.47923139570293216 2116 56-57 0.4160738318071182 2116 58-59 -0.5626216843506526 2116 60-61 -0.8941374505888433 2116 62-63 0.8100627634772337 2116 64-65 1.417353857640876 2116 66-67 0.25356882259259095 2116 68-69 0.776967601408856 2116 70-71 -3.3639085260700625 2116 72-73 1.1115325968876917 2116 74-75 -1.4282429203629405 2116 76 -0.9429139513888742 2117 1 0.10254504979769052 2117 2 0.506702162682064 2117 3 0.6767327195920885 2117 4 0.2679462516010105 2117 5 -1.0723633612496108 2117 6 -1.8731507500686106 2117 7 -3.4172424837689057 2117 8 -4.293538275795214 2117 9 -0.7282018665775709 2117 10-11 -0.5403074447919707 2117 12-13 0.8047829676981166 2117 14-15 -0.5208480461289184 2117 16-17 0.01601722066600786 2117 18-19 -0.1837294845057471 2117 20-21 -0.4710812057390754 2117 22-23 -0.4317410672532702 2117 24-25 0.2882362216023324 2117 26-27 0.6227267568891932 2117 28-29 0.036582007827014706 2117 30-31 -0.3171895219092917 2117 32-33 0.8876140641792887 2117 34-35 0.18156788259030066 2117 36-37 -1.923164200169353 2117 38-39 0.27243358664177464 2117 40-41 -1.2269788848297836 2117 42-43 -1.0724005032222266 2117 44-45 0.014263434876490066 2117 46-47 -0.8513249122572262 2117 48-49 -0.8101657879520516 2117 50-51 0.36692515412092774 2117 52-53 1.0690524505168497 2117 54-55 -1.2909281441277614 2117 56-57 -1.784449827841243 2117 58-59 -0.4430278597359347 2117 60-61 1.1164985381239845 2117 62-63 -0.46356296910362005 2117 64-65 -0.5074073752655721 2117 66-67 -2.5303266082734766 2117 68-69 -0.5517441415909303 2117 70-71 0.6084161971771707 2117 72-73 1.0413269315957479 2117 74-75 0.12263753329453664 2117 76 -0.2183507866395793 2118 1 -0.21275113910040488 2118 2 0.06127962747079607 2118 3 0.07430935588040555 2118 4 0.2509102118329878 2118 5 -0.3935108343233473 2118 6 0.9724892665013982 2118 7 0.9582235477141126 2118 8 -4.79390074472645 2118 9 -0.5368182708857745 2118 10-11 -1.0370711150488106 2118 12-13 0.73661291798809 2118 14-15 -1.226859852259821 2118 16-17 -0.6604533675692892 2118 18-19 -1.1891924091122092 2118 20-21 -3.3014716365592953 2118 22-23 -0.7068290954222789 2118 24-25 -0.37505603191879544 2118 26-27 0.6190761769389042 2118 28-29 -0.21095838156818303 2118 30-31 -0.6365764316027551 2118 32-33 -0.33033332604441057 2118 34-35 0.34908031009651097 2118 36-37 -0.7645322614783794 2118 38-39 -0.6084953694477093 2118 40-41 -1.5743794647800726 2118 42-43 -0.41006620330507815 2118 44-45 0.5972170388532945 2118 46-47 -1.3633640589017944 2118 48-49 -1.4518393587888312 2118 50-51 -1.075390504536898 2118 52-53 -2.456216812117784 2118 54-55 -0.04993808613273387 2118 56-57 1.0972299567486488 2118 58-59 0.5654901601480731 2118 60-61 -2.4095992249248965 2118 62-63 -0.00698156893792401 2118 64-65 -0.416997267560518 2118 66-67 -2.7811292180497844 2118 68-69 0.8638523787073353 2118 70-71 -2.0253866197242374 2118 72-73 -3.107647799141624 2118 74-75 -0.8299567086275843 2118 76 -0.9251134212708969 2119 1 -0.22851237317149753 2119 2 -0.042901011921109955 2119 3 0.0820781911140287 2119 4 0.38063299296422315 2119 5 0.3430194585449726 2119 6 1.2554090071676143 2119 7 -2.2851813259724523 2119 8 0.4121690071179529 2119 9 -0.6194250225066043 2119 10-11 0.36961902579626127 2119 12-13 0.4811076642434031 2119 14-15 -0.7774131721793296 2119 16-17 -0.6981517802677004 2119 18-19 -1.0550821922555116 2119 20-21 -2.5641309256270333 2119 22-23 0.43085813909593185 2119 24-25 -0.56841060379449 2119 26-27 0.12759953933550605 2119 28-29 0.5709832683312115 2119 30-31 -0.2351400354480475 2119 32-33 -0.6375376631783212 2119 34-35 0.07460009547545354 2119 36-37 -0.6887162543243477 2119 38-39 -0.5583612312854029 2119 40-41 -0.7662529277803003 2119 42-43 -1.1610442940718997 2119 44-45 -1.7268663503709405 2119 46-47 -0.6382355098295847 2119 48-49 -1.5182656945813733 2119 50-51 0.5595021849332582 2119 52-53 -2.4198597810424403 2119 54-55 -2.1549570890390584 2119 56-57 0.3180828518973158 2119 58-59 1.1692795632332462 2119 60-61 -0.07456121911224045 2119 62-63 -0.9157396731185656 2119 64-65 -0.08595546116659847 2119 66-67 0.12329734130635472 2119 68-69 -1.3786582405638512 2119 70-71 -1.32015523139426 2119 72-73 -2.2979004260139675 2119 74-75 -1.0074103191797867 2119 76 -0.5799871078347252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 10.0 13 63.0 14 174.0 15 431.0 16 568.0 17 769.0 18 841.0 19 855.0 20 783.0 21 813.0 22 823.0 23 928.0 24 1018.0 25 1289.0 26 1551.0 27 1879.0 28 2261.0 29 2685.0 30 3344.0 31 4039.0 32 5131.0 33 6763.0 34 9346.0 35 13565.0 36 21903.0 37 23314.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.65727429557217 21.747172704619512 11.459651140502205 28.135901859306117 2 18.214153249198755 19.253630562344863 35.11398111251653 27.418235075939855 3 17.67111432348382 23.156663401458882 29.879504322437683 29.29271795261962 4 13.502743725570381 18.669697286707436 36.385510085687926 31.44204890203426 5 13.30017403874502 36.03838362704353 35.730249455534526 14.931192878676924 6 28.86570485691733 36.32369304510742 17.016804724723965 17.79379737325129 7 27.832884763526046 29.9907749954826 22.135255684790156 20.0410845562012 8 23.734890488735033 32.86193877259888 19.214638275209463 24.188532463456617 9 23.334506272052042 16.040095483551912 21.175665008701937 39.44973323569411 10-11 24.464331567585045 25.51569677315048 26.840007988663707 23.179963670600767 12-13 23.995948606263493 23.682108246393213 26.308381439671326 26.013561707671972 14-15 21.55465101903014 25.29505748984774 23.793854435134904 29.356437055987218 16-17 22.19184205270616 27.528079201894457 26.419176597019465 23.860902148379918 18-19 21.355409942082186 26.886608526947477 27.12293982824373 24.635041702726607 20-21 21.030157205489353 27.3554670039658 26.790078840502524 24.82429695004232 22-23 22.64310644894388 26.85902861653463 26.686891934302754 23.810973000218738 24-25 21.75674519015873 27.988853912067636 26.522363503219243 23.732037394554396 26-27 20.628821957412814 28.099649069415783 27.31457265404331 23.956956319128096 28-29 21.748661423313585 27.241343236740246 28.134837230976995 22.875158108969178 30-31 21.8599320963585 27.10962538873408 27.71448135502953 23.315961159877887 32-33 20.643087428316008 27.833835794919594 27.576106287268544 23.946970489495857 34-35 22.064403845970958 27.263692474488582 27.47577247524941 23.196131204291053 36-37 22.21609335324159 27.001207809869804 26.694975701147893 24.08772313574071 38-39 21.522791467346337 27.31742574822395 27.97411292546767 23.185669858962044 40-41 21.74913693901036 26.172383950394202 28.0178603695708 24.06061874102464 42-43 20.99544455962491 25.94698951012373 28.318861805628202 24.738704124623155 44-45 20.79097281001246 25.288400270092914 29.08919723440071 24.83142968549392 46-47 20.632626082987 25.17855614413832 29.589915263102835 24.598902509771847 48-49 19.487584285157254 25.70400098907265 30.311272575107708 24.497142150662395 50-51 19.31592311862215 26.367345386071193 29.62177481478664 24.694956680520026 52-53 18.949300516410048 26.168579824820014 29.938943784534327 24.94317587423561 54-55 18.179916119031088 26.84951830259917 29.253250149787448 25.7173154285823 56-57 18.288333697895368 26.650752741347993 29.440127818619295 25.620785742137347 58-59 18.650201143139736 26.218033457284424 28.90802575393014 26.223739645645704 60-61 18.90269997812628 25.984555250168807 28.938458758523616 26.174286013181298 62-63 19.255532625131956 25.95126915139469 28.44867759084727 26.344520632626082 64-65 20.113838457807493 25.69544170653073 27.35071184699807 26.840007988663707 66-67 19.685398815014885 25.954122245575327 26.781995073657384 27.578483865752407 68-69 19.572701594879646 25.402999553015242 27.044479738276163 27.979819113828945 70-71 20.7477008816061 25.804334801091784 26.29411596876813 27.153848348533987 72-73 20.373945543942405 26.024023053000978 26.125307896413663 27.47672350664295 74-75 20.35682697885857 27.25608422334021 26.325024489058386 26.062064308742833 76 20.06866446661404 26.759170320212274 26.451036148703267 26.721129064470418 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.5 14 4.0 15 5.0 16 4.0 17 5.0 18 8.0 19 9.5 20 22.0 21 36.0 22 50.0 23 74.0 24 103.5 25 123.0 26 173.5 27 280.0 28 402.0 29 468.0 30 612.0 31 901.5 32 1235.0 33 1423.0 34 1690.5 35 2249.5 36 2833.5 37 3126.0 38 3537.5 39 4419.0 40 5380.0 41 6142.5 42 6414.0 43 6692.0 44 7332.5 45 7604.5 46 7514.0 47 7641.5 48 7633.5 49 7181.0 50 6864.0 51 6297.0 52 5184.5 53 4295.0 54 3951.0 55 3633.5 56 2913.5 57 2233.5 58 1956.0 59 1636.0 60 1186.0 61 935.0 62 814.0 63 715.5 64 535.0 65 401.5 66 307.0 67 264.0 68 219.5 69 144.5 70 92.5 71 71.0 72 62.0 73 42.0 74 24.0 75 17.0 76 14.0 77 9.0 78 6.0 79 5.0 80 4.0 81 4.5 82 4.0 83 2.0 84 1.5 85 2.0 86 2.5 87 2.0 88 2.5 89 2.0 90 1.5 91 1.5 92 1.0 93 0.5 94 0.5 95 1.5 96 2.0 97 1.0 98 1.5 99 4.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7693843973789575 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 105149.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.39806370008274 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96205861522377 42.27714956870726 2 10.704529358200398 12.074294572463836 3 5.200499139995278 8.798942453090376 4 2.8565647027081718 6.444188722669736 5 1.7705979562240732 4.99291481611808 6 1.0522410711274492 3.5606615374373507 7 0.807729924791744 3.1888082625607472 8 0.5598462109203737 2.525939381258975 9 0.3878452666014637 1.9686349846408429 >10 1.6964014704394454 14.11996309998193 >50 0.0016862837678324508 0.048502601070861344 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007608251148370408 0.0 2 0.0 0.0 0.0 0.008559282541916709 0.0 3 0.0 0.0 0.0 0.013314439509648213 0.0 4 0.0 0.0 0.0 0.017118565083833418 0.0 5 0.0 0.0 0.0 0.017118565083833418 0.0 6 0.0 0.0 0.0 0.017118565083833418 0.0 7 0.0 0.0 0.0 0.017118565083833418 0.0 8 0.0 0.0 0.0 0.01806959647737972 0.0 9 0.0 0.0 0.0 0.035188161561213134 0.0 10 0.0 0.0 0.0 0.03804125574185204 0.0 11 0.0 0.0 0.0 0.039943318528944644 0.0 12 0.0 0.0 0.0 0.04089434992249094 0.0 13 0.0 0.0 0.0 0.04089434992249094 0.0 14 0.0 0.0 0.0 0.04945363246440765 0.0 15 0.0 0.0 0.0 0.05420878943213916 0.0 16 0.0 0.0 0.0 0.055159820825685456 0.0 17 0.0 0.0 0.0 0.06086600918696326 0.0 18 0.0 0.0 0.0 0.07227838590951888 0.0 19 0.0 0.0 0.0 0.07608251148370408 0.0 20 0.0 0.0 0.0 0.08273973123852818 0.0 21 0.0 0.0 0.0 0.08369076263207448 0.0 22 0.0 0.0 0.0 0.08939695099335229 0.0 23 0.0 0.0 0.0 0.0941521079610838 0.0 24 0.0 0.0 0.0 0.10556448468363941 0.0 25 0.0 0.0 0.0 0.1065155160771857 0.0 26 0.0 0.0 0.0 0.11697686140619502 0.0 27 0.0 0.0 0.0 0.14170367763839883 0.0 28 0.0 0.0 0.0 0.16833255665769528 0.0 29 0.0 0.0 0.0 0.214933094941464 0.0 30 0.0 0.0 0.0 0.26058260183168647 0.0 31 0.0 0.0 0.0 0.3014769517541774 0.0 32 0.0 0.0 0.0 0.34142027028312205 0.0 33 0.0 0.0 0.0 0.3851677143862519 0.0 34 0.0 0.0 0.0 0.4717115711989653 0.0 35 0.0 0.0 0.0 0.5829822442438825 0.0 36 0.0 0.0 0.0 0.7646292404112259 0.0 37 0.0 0.0 0.0 1.2334877174295524 0.0 38 0.0 0.0 0.0 1.7993513965896015 0.0 39 0.0 0.0 0.0 2.41942386518179 0.0 40 0.0 0.0 0.0 3.2458701461735253 0.0 41 0.0 0.0 0.0 4.244453109397141 0.0 42 0.0 0.0 0.0 5.5207372395362775 0.0 43 0.0 0.0 0.0 6.8455239707462745 0.0 44 0.0 0.0 0.0 7.912581194305224 0.0 45 0.0 0.0 0.0 9.043357521231776 0.0 46 0.0 0.0 0.0 10.154162188893855 0.0 47 0.0 0.0 0.0 11.126116273098175 0.0 48 0.0 0.0 0.0 12.01723268885106 0.0 49 0.0 0.0 0.0 12.98633367887474 0.0 50 0.0 0.0 0.0 13.887911439956634 0.0 51 0.0 0.0 0.0 14.816118080057823 0.0 52 0.0 0.0 0.0 15.87366498968131 0.0 53 0.0 0.0 0.0 16.920750553975786 0.0 54 0.0 0.0 0.0 17.98210158917346 0.0 55 0.0 0.0 0.0 18.87131594213925 0.0 56 0.0 0.0 0.0 19.772893703221143 0.0 57 0.0 0.0 0.0 20.515649221580805 0.0 58 0.0 0.0 0.0 21.300250121256504 0.0 59 0.0 0.0 0.0 22.02018088617105 0.0 60 0.0 0.0 0.0 22.723944117395316 0.0 61 0.0 0.0 0.0 23.405833626568015 0.0 62 0.0 0.0 0.0 24.18282627509534 0.0 63 0.0 0.0 0.0 24.90751219697762 0.0 64 0.0 0.0 0.0 25.695917222227507 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCCGT 20 0.00693736 52.494648 59 GTTCTAC 20 0.00693736 52.494648 33 ATACTGG 20 0.00693736 52.494648 6 CTGGGTA 20 0.00693736 52.494648 31 GATTCAA 20 0.00693736 52.494648 9 AGTGCGC 35 0.0012511981 39.995922 8 TCATGTC 35 0.0012511981 39.995922 36 AACTTCC 40 0.0024106847 34.996433 22 AGAAAAG 40 0.0024106847 34.996433 20 TCCACGA 40 0.0024106847 34.996433 12 GGGTTCA 40 0.0024106847 34.996433 19 TTTGAGG 40 0.0024106847 34.996433 35 GACTGTT 40 0.0024106847 34.996433 6 TGTGAAA 55 2.9768972E-4 31.814938 10 TGCTTGT 45 0.004292987 31.107937 31 ACCAGTG 45 0.004292987 31.107937 43 GTGTATT 45 0.004292987 31.107937 26 CTTTGAG 45 0.004292987 31.107937 34 CCTATTG 45 0.004292987 31.107937 2 CTAGACC 50 0.00718464 27.997145 3 >>END_MODULE