FastQCFastQC Report
Wed 25 May 2016
SRR1780648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105149
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT11631.106049510694348No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT4740.4507888805409466RNA PCR Primer, Index 24 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1520.14455677181903775No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT207.8952E-570.0000134
GTCCGGA150.002219268870.0000133
CAATACG207.8952E-570.0000169
CAACGGA252.3810209E-455.99999664
AACGGAA252.3810209E-455.99999665
ACGGAAC252.3810209E-455.99999666
TCCGTAC252.3810209E-455.99999640
CATCGTT252.3810209E-455.99999632
ACCAACG252.3810209E-455.99999662
ATCATTG252.3810209E-455.99999622
TGCATAA200.00693456352.50000430
CCGTACT200.00693456352.50000441
TACGAGT200.00693456352.5000044
TCAGATG200.00693456352.5000043
CTCGCTA200.00693456352.50000413
AACTCGT200.00693456352.50000437
GGACCAG200.00693456352.50000442
ACAAGTA200.00693456352.50000430
CGGAACA305.85492E-446.66666867
ACCCTTT305.85492E-446.66666811
Produced by FastQC (version 0.11.5)