##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780648_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 105149 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.049548735603764 34.0 34.0 34.0 32.0 34.0 2 33.36118270264102 34.0 34.0 34.0 33.0 34.0 3 33.50289589059335 34.0 34.0 34.0 34.0 34.0 4 33.52489324672607 34.0 34.0 34.0 34.0 34.0 5 33.520822832361695 34.0 34.0 34.0 34.0 34.0 6 37.26635536239051 38.0 38.0 38.0 36.0 38.0 7 36.853474593196324 38.0 37.0 38.0 35.0 38.0 8 36.909376218508974 38.0 37.0 38.0 35.0 38.0 9 36.71691599539701 38.0 37.0 38.0 34.0 38.0 10-11 36.422643106448945 38.0 37.0 38.0 34.0 38.0 12-13 36.31603724238937 38.0 37.0 38.0 34.0 38.0 14-15 36.574484778742544 38.0 37.0 38.0 34.0 38.0 16-17 36.69165184642745 38.0 37.0 38.0 34.0 38.0 18-19 36.71588412633501 38.0 37.0 38.0 34.0 38.0 20-21 36.45013742403637 38.0 37.0 38.0 34.0 38.0 22-23 36.52868786198633 38.0 37.0 38.0 34.0 38.0 24-25 35.864929766331585 38.0 36.5 38.0 32.5 38.0 26-27 36.095721309760435 38.0 37.0 38.0 33.5 38.0 28-29 35.96487841063633 38.0 37.0 38.0 32.5 38.0 30-31 35.70642136396923 38.0 36.5 38.0 31.5 38.0 32-33 36.20923641689413 38.0 37.0 38.0 34.0 38.0 34-35 36.25851886370769 38.0 37.0 38.0 34.0 38.0 36-37 36.39919542744106 38.0 37.0 38.0 34.0 38.0 38-39 36.03949633377398 38.0 37.0 38.0 32.5 38.0 40-41 35.56361924507128 38.0 36.0 38.0 31.0 38.0 42-43 35.926166677762026 38.0 36.5 38.0 32.5 38.0 44-45 36.13744305699531 38.0 37.0 38.0 34.0 38.0 46-47 36.427693083148675 38.0 37.0 38.0 34.0 38.0 48-49 36.24117205108941 38.0 37.0 38.0 34.0 38.0 50-51 36.37650857354801 38.0 37.0 38.0 34.0 38.0 52-53 36.29575174276503 38.0 37.0 38.0 34.0 38.0 54-55 36.48203026181894 38.0 37.0 38.0 34.0 38.0 56-57 36.55239231947047 38.0 37.0 38.0 34.0 38.0 58-59 36.59843650438901 38.0 37.0 38.0 34.0 38.0 60-61 36.29316969253155 38.0 37.0 38.0 34.0 38.0 62-63 36.54735185308467 38.0 37.0 38.0 34.0 38.0 64-65 36.53854054722346 38.0 37.0 38.0 34.0 38.0 66-67 35.96802632454897 38.0 37.0 38.0 33.0 38.0 68-69 35.45167333973694 38.0 36.0 38.0 31.0 38.0 70-71 35.47077480527632 38.0 36.0 38.0 31.0 38.0 72-73 35.19496143567699 38.0 36.0 38.0 29.0 38.0 74-75 35.7907302970071 38.0 36.5 38.0 32.0 38.0 76 35.18503266792837 38.0 36.0 38.0 28.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5239825599584478 1101 2 0.45715120697667544 1101 3 0.21075197763785525 1101 4 0.3979044952381514 1101 5 0.1516479134486417 1101 6 -0.9969263099243264 1101 7 -0.019560655450924003 1101 8 0.6184825176922644 1101 9 0.2980411013016848 1101 10-11 0.3051619516099606 1101 12-13 0.9298748670108168 1101 14-15 0.6140910912122521 1101 16-17 -0.13522593846810338 1101 18-19 -0.28262488267197483 1101 20-21 0.193348269991219 1101 22-23 0.6447825278501682 1101 24-25 -1.1065217215086705 1101 26-27 -0.11216179580922159 1101 28-29 0.5993232361370886 1101 30-31 1.0970003755190874 1101 32-33 0.42463119085586953 1101 34-35 0.6828341835591232 1101 36-37 0.41493476029688026 1101 38-39 0.43600895898782 1101 40-41 1.2549548747198926 1101 42-43 0.8724349720786719 1101 44-45 0.6148836963540774 1101 46-47 0.7688358597105207 1101 48-49 -0.9916842744192991 1101 50-51 -1.3239373877575034 1101 52-53 -1.6385626340761164 1101 54-55 -0.5524978745791032 1101 56-57 -0.9117543271727584 1101 58-59 -1.0242021321269235 1101 60-61 -1.5415037316243385 1101 62-63 -0.8109727507567683 1101 64-65 -1.2673633730778704 1101 66-67 -0.9793113370749751 1101 68-69 -0.8281622713685834 1101 70-71 -0.6683884754512235 1101 72-73 -1.8987475558030695 1101 74-75 -1.1500513833293908 1101 76 -1.1488091174203987 1102 1 -1.2398747386862006 1102 2 0.022239226917477595 1102 3 0.18348916411549965 1102 4 0.3645036539856221 1102 5 0.17797600193594576 1102 6 0.33016513734160213 1102 7 -0.11991896671436564 1102 8 0.476653572373678 1102 9 0.30276146272869653 1102 10-11 0.576271158651565 1102 12-13 0.6181830146973724 1102 14-15 -0.13544933564551087 1102 16-17 0.2642677521379113 1102 18-19 -0.1609158937328914 1102 20-21 0.5733918904928572 1102 22-23 0.5594966577769469 1102 24-25 -4.399978646263307 1102 26-27 -0.8867372748558111 1102 28-29 -0.20802993413865067 1102 30-31 1.5519310500789842 1102 32-33 0.048443311123818944 1102 34-35 0.20247119867050856 1102 36-37 -2.525756936229058 1102 38-39 -0.2645908229096747 1102 40-41 0.8098076555268605 1102 42-43 0.7855912269470267 1102 44-45 0.8283203687329532 1102 46-47 0.9466984551303597 1102 48-49 -2.9619600183046373 1102 50-51 -0.43213181056479044 1102 52-53 -0.14825261693948732 1102 54-55 0.3949627890756773 1102 56-57 0.7234302810216633 1102 58-59 0.035916266377519435 1102 60-61 -5.302276437653315 1102 62-63 -2.2686297066689036 1102 64-65 -0.17237973076598223 1102 66-67 1.009027968112747 1102 68-69 -0.7147645458661671 1102 70-71 -1.4552166418322798 1102 72-73 -5.986728549727353 1102 74-75 -2.314882353885551 1102 76 0.14704693492419807 1103 1 0.6226950650947316 1103 2 0.3011725739127087 1103 3 0.013244786842072642 1103 4 0.23983025883217834 1103 5 0.06234507822058788 1103 6 -0.0016653867631148955 1103 7 0.5926431522466515 1103 8 0.47903586654858543 1103 9 0.024955222654924114 1103 10-11 -0.12207404345920736 1103 12-13 -1.0851573203607714 1103 14-15 -0.4764376034870992 1103 16-17 0.01545889922536503 1103 18-19 0.3275312603221323 1103 20-21 -0.5216565238726361 1103 22-23 0.3801201528330438 1103 24-25 -2.962609928603584 1103 26-27 -0.3944861349408555 1103 28-29 -0.6465129506866347 1103 30-31 0.7142856789021863 1103 32-33 1.0190360669002416 1103 34-35 0.12602004850912607 1103 36-37 -0.7242174106386088 1103 38-39 -0.06379928645801414 1103 40-41 -0.5382378386070528 1103 42-43 -0.30648572371642757 1103 44-45 0.6928113774936477 1103 46-47 0.7030640476299794 1103 48-49 -1.9801858495459896 1103 50-51 -0.723540181895288 1103 52-53 0.11468349659091359 1103 54-55 -0.2440812193263895 1103 56-57 0.1604275657186207 1103 58-59 -0.46008762726457064 1103 60-61 -3.957727536582567 1103 62-63 -1.347002061810045 1103 64-65 -0.11261744786214933 1103 66-67 -0.6092798345410841 1103 68-69 0.14788210611394703 1103 70-71 0.4339810629681722 1103 72-73 -5.22360031569897 1103 74-75 -2.099092610558536 1103 76 -3.4290583983408247 1104 1 -0.04434960049194103 1104 2 0.13329476784929994 1104 3 0.3240524702486951 1104 4 0.026546296786357004 1104 5 -0.14279262138597915 1104 6 0.10863315524105133 1104 7 0.5085885352598396 1104 8 0.5038449385226613 1104 9 0.8876259007387048 1104 10-11 -0.3351265775948207 1104 12-13 0.5073907009305927 1104 14-15 0.5412550602481474 1104 16-17 0.2571737800399916 1104 18-19 0.47142156349269015 1104 20-21 -0.49778473493488207 1104 22-23 0.0929528304539673 1104 24-25 0.6011137464716256 1104 26-27 0.9417439044018963 1104 28-29 1.338837662597335 1104 30-31 0.7899278913530097 1104 32-33 0.4983116975412969 1104 34-35 0.026043191300146873 1104 36-37 0.9955118187064542 1104 38-39 1.3347774318942243 1104 40-41 1.6603735939317161 1104 42-43 0.6376707215508688 1104 44-45 0.5299786998727214 1104 46-47 0.896422066607073 1104 48-49 0.13994837864192533 1104 50-51 0.7642751386323638 1104 52-53 0.3998258247556947 1104 54-55 0.11850845898881346 1104 56-57 -0.011789513661142337 1104 58-59 -0.5114646233302977 1104 60-61 0.478271074849971 1104 62-63 0.41777461025660045 1104 64-65 0.754311503769415 1104 66-67 0.1414838775625995 1104 68-69 1.5888679890114261 1104 70-71 1.6305559298971275 1104 72-73 1.3161011422968585 1104 74-75 0.4110323605129764 1104 76 0.246757385042649 1105 1 0.7401447295242605 1105 2 0.2911054598187519 1105 3 0.24814411570113037 1105 4 -0.04540464049667747 1105 5 -0.29671532446397464 1105 6 0.04531447900869523 1105 7 0.7168042260721563 1105 8 -0.9068701737198737 1105 9 0.5551565649368015 1105 10-11 0.4282615270105907 1105 12-13 -0.6958955753943386 1105 14-15 0.14604561798940807 1105 16-17 0.2872709797622761 1105 18-19 0.5775312603221323 1105 20-21 0.30720253653005614 1105 22-23 0.28616042128942354 1105 24-25 0.5508128901883538 1105 26-27 0.17430581136786572 1105 28-29 0.895433358038197 1105 30-31 1.0515340010498377 1105 32-33 0.9150092212626646 1105 34-35 -0.7447853206183837 1105 36-37 0.5509503745962903 1105 38-39 0.057006082669509794 1105 40-41 0.7134400137419377 1105 42-43 0.5391518601761973 1105 44-45 0.947844934540619 1105 46-47 0.9983660610527991 1105 48-49 0.13726381488353212 1105 50-51 0.8737752543463202 1105 52-53 0.5576365167251467 1105 54-55 0.9723617336937451 1105 56-57 0.4607631361884259 1105 58-59 0.49461035931260966 1105 60-61 1.0053597117395796 1105 62-63 0.02548115966646236 1105 64-65 0.7766442971042977 1105 66-67 0.6390423131099254 1105 68-69 0.1512378108119421 1105 70-71 1.0950548884715303 1105 72-73 1.226064113831228 1105 74-75 0.21298792635421648 1105 76 0.9568476419276308 1106 1 -0.40029401634371453 1106 2 0.14420573027853578 1106 3 0.18676477081482545 1106 4 0.03480671881415276 1106 5 0.24112400239167897 1106 6 0.2910572417052819 1106 7 -1.1178396685487044 1106 8 0.33343835077176465 1106 9 -0.8065583504193015 1106 10-11 0.38466240850914346 1106 12-13 1.1047530272301245 1106 14-15 -0.31462502209874543 1106 16-17 0.3288541711877002 1106 18-19 0.7131868748668566 1106 20-21 0.3340732166863205 1106 22-23 0.4326594696716768 1106 24-25 0.08281729768108903 1106 26-27 0.95168986425778 1106 28-29 0.5281840341876531 1106 30-31 0.6429644327837849 1106 32-33 0.15368997855004807 1106 34-35 0.5001811223346309 1106 36-37 0.9589189211253952 1106 38-39 0.08947377775114518 1106 40-41 1.0102320602209502 1106 42-43 0.7420968666872554 1106 44-45 0.9984810158789017 1106 46-47 0.8897313820985744 1106 48-49 1.1127095726717755 1106 50-51 0.3179678818130185 1106 52-53 0.599169623005821 1106 54-55 0.07193100144893094 1106 56-57 0.32400564845929125 1106 58-59 0.5553135547448491 1106 60-61 0.7531810079431267 1106 62-63 0.4932764115947421 1106 64-65 -0.7533441833124144 1106 66-67 0.223110228566199 1106 68-69 1.3412483286923376 1106 70-71 0.08612721142054625 1106 72-73 0.6347964588177035 1106 74-75 -0.4092198268261882 1106 76 0.27784333460518695 1107 1 0.6290608703523688 1107 2 -0.5426589496441139 1107 3 0.18085164541194132 1107 4 0.11256989628229519 1107 5 -0.023007995426539196 1107 6 0.23174004033670315 1107 7 0.6711844798124815 1107 8 0.009939565627718139 1107 9 -0.22193991436039084 1107 10-11 0.355233142877168 1107 12-13 0.1866380373952481 1107 14-15 0.21380632614343398 1107 16-17 0.5234356657084476 1107 18-19 0.42027022886100696 1107 20-21 0.7365376387619875 1107 22-23 -0.02090328135723496 1107 24-25 0.4572656539066102 1107 26-27 -0.15487655613195983 1107 28-29 0.6573678493312016 1107 30-31 0.2349215905167128 1107 32-33 0.7547213664609984 1107 34-35 -0.05546671819693216 1107 36-37 0.971935234905331 1107 38-39 0.39747075300775947 1107 40-41 0.42738012388932844 1107 42-43 0.5712484509585991 1107 44-45 0.41858564224870776 1107 46-47 0.6352810424347695 1107 48-49 0.2999151560505595 1107 50-51 0.7702723891765331 1107 52-53 0.47221097769417497 1107 54-55 0.4781143703188633 1107 56-57 0.5687867264580007 1107 58-59 0.4338899419119997 1107 60-61 0.9406035298849105 1107 62-63 0.4514382601592217 1107 64-65 0.39808513190551764 1107 66-67 -0.3641238667380051 1107 68-69 0.5122269075588051 1107 70-71 -0.17607713230931665 1107 72-73 0.9292068817859231 1107 74-75 -0.8068620145586252 1107 76 0.9953769290912291 1108 1 -0.7251344605871424 1108 2 0.20239960936793722 1108 3 0.11262787951375941 1108 4 0.26261967423904764 1108 5 -0.02329883292667745 1108 6 -0.30385958828617987 1108 7 -0.20381132541588443 1108 8 -0.5936936659124896 1108 9 -0.19130130232642983 1108 10-11 0.3670735849501696 1108 12-13 0.9762226990729346 1108 14-15 0.49520838661526767 1108 16-17 -0.18252202862133515 1108 18-19 0.03301450439597886 1108 20-21 0.7962823729253259 1108 22-23 -0.027614915975846088 1108 24-25 0.8029539879670438 1108 26-27 -0.7661163069065964 1108 28-29 -1.0662302579878329 1108 30-31 0.04906524186966266 1108 32-33 0.4758920992596387 1108 34-35 0.3966457687992815 1108 36-37 0.826801729306716 1108 38-39 0.8819445049233607 1108 40-41 0.8168115365765445 1108 42-43 -0.5031164606156224 1108 44-45 0.4246205203766138 1108 46-47 0.6328089236835837 1108 48-49 0.8758069645005122 1108 50-51 0.3677756610984062 1108 52-53 -0.2591838118401455 1108 54-55 -0.389850997646505 1108 56-57 0.02808625552496835 1108 58-59 0.20030827809777918 1108 60-61 0.3802100043751082 1108 62-63 0.5695742155044812 1108 64-65 0.28780738209287904 1108 66-67 0.3550389687038944 1108 68-69 0.9664367803733214 1108 70-71 -0.6775480988965583 1108 72-73 -0.7656822084520059 1108 74-75 -0.9335049667829765 1108 76 0.5801952115839271 1109 1 -0.8052691407218262 1109 2 0.2334097103780337 1109 3 0.3597995966854768 1109 4 0.2780405496599201 1109 5 0.05858668895884733 1109 6 0.006679132252543241 1109 7 0.04628968468512795 1109 8 0.3963736741548516 1109 9 0.7364764754513473 1109 10-11 0.6527469856235655 1109 12-13 0.7566435744938076 1109 14-15 0.871370002776878 1109 16-17 0.8491278030284946 1109 18-19 0.7356744370559127 1109 20-21 1.020491127134079 1109 22-23 0.7091022557413282 1109 24-25 0.1332401832532355 1109 26-27 0.5796749388846507 1109 28-29 -0.3438060155635867 1109 30-31 0.6530888108932373 1109 32-33 0.9761277894953224 1109 34-35 -0.08648554433203515 1109 36-37 1.02143135893634 1109 38-39 1.0936280066068704 1109 40-41 0.8787643985294054 1109 42-43 1.0978431353439717 1109 44-45 0.7988292745853656 1109 46-47 0.8771691930438479 1109 48-49 0.9849483786419242 1109 50-51 0.4223211156438609 1109 52-53 0.008274100617768454 1109 54-55 -0.29045705825257073 1109 56-57 0.3674633599020751 1109 58-59 0.5023284801179742 1109 60-61 1.0514894656545692 1109 62-63 0.4848435757738443 1109 64-65 0.9078747221602228 1109 66-67 0.9047022683672026 1109 68-69 0.3138105177470649 1109 70-71 0.7281421367936787 1109 72-73 0.43305516528536714 1109 74-75 -0.2186228119008149 1109 76 0.5492861206748358 1110 1 -0.4355721710248588 1110 2 -0.29591688894856105 1110 3 -0.1567323898465105 1110 4 -0.09091567929630884 1110 5 0.3134015037736617 1110 6 0.03957475514816622 1110 7 0.462855341250787 1110 8 0.6689325967137734 1110 9 0.90297479194966 1110 10-11 -0.6514449335683423 1110 12-13 0.3752125980628307 1110 14-15 0.7763531677600426 1110 16-17 0.7585554124561042 1110 18-19 0.12897410035557755 1110 20-21 0.6565517331946893 1110 22-23 -0.7111165994775277 1110 24-25 -0.691995506982451 1110 26-27 0.8921328513425593 1110 28-29 0.20481351305593876 1110 30-31 -0.20851051570608803 1110 32-33 0.5095621329296733 1110 34-35 0.9202147923682986 1110 36-37 1.0793269818319615 1110 38-39 0.9610690840479421 1110 40-41 -1.4996867799217668 1110 42-43 0.027186569687408735 1110 44-45 0.6569437526998456 1110 46-47 0.5688358597105179 1110 48-49 0.9431470318405815 1110 50-51 -0.718082924760175 1110 52-53 0.5674155147591762 1110 54-55 -0.48917759697310714 1110 56-57 0.47421420165291295 1110 58-59 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2116 38-39 -4.401734847021668 2116 40-41 -1.1853192423069956 2116 42-43 -0.9551273443608252 2116 44-45 -0.0973084867922509 2116 46-47 -0.6839316679647567 2116 48-49 0.17555723473049056 2116 50-51 -1.2281093886611814 2116 52-53 -0.11056353775862249 2116 54-55 -0.7530400840828264 2116 56-57 -0.16519961672769767 2116 58-59 -2.186232405490877 2116 60-61 -1.6480554297943826 2116 62-63 0.3505077824159173 2116 64-65 -1.548017036757365 2116 66-67 -0.7149164278197588 2116 68-69 -0.8410849312074262 2116 70-71 0.6356329117014212 2116 72-73 0.22306131534686813 2116 74-75 -0.022165247325169446 2116 76 -0.9707384795711675 2117 1 0.5751230161409211 2117 2 0.41983127900548567 2117 3 0.3126427339403719 2117 4 0.10005035358148717 2117 5 0.21549845366472908 2117 6 0.25736540676234654 2117 7 0.7131524297831646 2117 8 0.27823641925288456 2117 9 -0.743180387293755 2117 10-11 -0.39891968104309683 2117 12-13 -1.3334054451140318 2117 14-15 0.09360039493374472 2117 16-17 -0.8974408244224819 2117 18-19 -1.2028304649048707 2117 20-21 0.03210877419290625 2117 22-23 0.18331794201583307 2117 24-25 -1.4178137383153882 2117 26-27 -1.4357662375859448 2117 28-29 -0.632016799877313 2117 30-31 -0.1876709930283269 2117 32-33 -1.7903673085438925 2117 34-35 0.5773870046875729 2117 36-37 -0.7702843273381674 2117 38-39 0.48963290856764985 2117 40-41 0.09481832009803526 2117 42-43 0.18843137063809223 2117 44-45 -0.5772981763950256 2117 46-47 -0.7546935520541922 2117 48-49 -0.857110444887482 2117 50-51 -0.2781690804345729 2117 52-53 0.5797446888530544 2117 54-55 -0.4614864700172774 2117 56-57 0.2305025755883463 2117 58-59 -1.2704656375290782 2117 60-61 -0.32333896571797993 2117 62-63 -0.4727299536379235 2117 64-65 -0.7685713562711527 2117 66-67 -0.1865967512406428 2117 68-69 -0.08234144303725799 2117 70-71 0.008509783852503006 2117 72-73 1.1754571260696807 2117 74-75 1.1202742469227118 2117 76 1.2858547481258142 2118 1 0.06186700951291613 2118 2 0.2599824803310895 2118 3 -0.6655055676337724 2118 4 0.16855698158480692 2118 5 0.17464302698701317 2118 6 0.2922659867126356 2118 7 0.7699047081593093 2118 8 0.5480168666431098 2118 9 -1.4757300973186034 2118 10-11 -0.8038492585078885 2118 12-13 -1.3467132330179297 2118 14-15 0.6612526318848637 2118 16-17 -2.7123745443893412 2118 18-19 -0.3629640605966742 2118 20-21 -0.26288915455606343 2118 22-23 0.6446891102014192 2118 24-25 0.5997660048494851 2118 26-27 -2.738634920270279 2118 28-29 -0.5961064850471516 2118 30-31 -0.6518642407499513 2118 32-33 -0.2481914593309682 2118 34-35 -0.25157944104564933 2118 36-37 -3.390986481439249 2118 38-39 -0.4373813830644977 2118 40-41 -0.3259304371513423 2118 42-43 0.43615299449890443 2118 44-45 -2.098166030578952 2118 46-47 -0.07905146423314591 2118 48-49 0.457201899768684 2118 50-51 -0.9746272411636525 2118 52-53 -1.5720780120582916 2118 54-55 -1.0279218469849667 2118 56-57 0.04854317210864423 2118 58-59 -0.9040095480510288 2118 60-61 -0.876233539040264 2118 62-63 -1.3923395228177071 2118 64-65 0.07740523859214932 2118 66-67 0.25740179888363457 2118 68-69 -1.605461782722422 2118 70-71 -0.18833673644576265 2118 72-73 0.9278908460365471 2118 74-75 -0.02862281190081717 2118 76 -0.3604791375411267 2119 1 0.4756832402305591 2119 2 -0.9862728293045038 2119 3 -0.2852797683938917 2119 4 -0.5683086566892825 2119 5 -0.2196672792951233 2119 6 -0.32538435981094693 2119 7 -0.8841865057910638 2119 8 -0.23378505600388166 2119 9 -0.5920949531200819 2119 10-11 -0.5001101572335713 2119 12-13 -0.5987532509030231 2119 14-15 -0.5037093623024802 2119 16-17 -0.3865864826857859 2119 18-19 -0.07996048357900776 2119 20-21 -1.1677628411198882 2119 22-23 -0.14954853790946743 2119 24-25 1.0498274848405345 2119 26-27 0.4399923992021044 2119 28-29 -0.22856792032549578 2119 30-31 0.7490411918456203 2119 32-33 0.5550166783842201 2119 34-35 0.7720065191600227 2119 36-37 0.14293929544427186 2119 38-39 -1.5727211997423396 2119 40-41 -0.9065530617880526 2119 42-43 -0.004379086878252281 2119 44-45 0.06573403649012732 2119 46-47 -0.12362445774979847 2119 48-49 -0.8687817800882343 2119 50-51 0.6596227029454482 2119 52-53 0.11160743395109307 2119 54-55 0.10113024333473675 2119 56-57 -1.1433302908915834 2119 58-59 -0.31790961512012217 2119 60-61 0.2462513704164735 2119 62-63 -0.2348389639087003 2119 64-65 -0.0963316270461263 2119 66-67 0.44041655408148017 2119 68-69 0.7216676606042043 2119 70-71 -0.6323340536825128 2119 72-73 0.7350392922694908 2119 74-75 -0.3401307484087539 2119 76 -0.240872609483894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 3.0 22 4.0 23 18.0 24 29.0 25 65.0 26 162.0 27 257.0 28 507.0 29 832.0 30 1338.0 31 2221.0 32 3491.0 33 5428.0 34 8243.0 35 13401.0 36 24189.0 37 44961.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.20436713615916 22.25508564037699 11.298252955330055 27.242294268133794 2 19.068179440603334 19.142359889299946 36.87624228475782 24.9132183853389 3 18.45191109758533 23.55894968092897 29.676934635612323 28.31220458587338 4 14.175122920807615 18.274068226992174 37.37458273497608 30.176226117224132 5 13.618769555583029 36.465396722745815 36.060257349095096 13.855576372576058 6 29.865238851534485 38.11258309636801 17.21557028597514 14.80660776612236 7 28.244681356931594 29.520014455677178 22.631694072221322 19.6036101151699 8 24.37873874216588 35.24522344482591 20.334953256807005 20.0410845562012 9 23.881349323341166 15.880322209436134 21.734871468107162 38.50345699911554 10-11 25.054922062977298 25.8518863707691 27.08347202541156 22.009719540842042 12-13 24.69590771191357 23.02589658484627 26.92227220420546 25.355923499034706 14-15 21.82617048188761 24.939371748661422 23.848538740263816 29.385919029187153 16-17 23.12480384977508 28.133410683886673 25.759160809898336 22.98262465643991 18-19 22.60982035016976 27.37020799056577 26.734919019676838 23.285052639587633 20-21 21.943147343293802 27.07633928995996 26.601299108883588 24.379214257862653 22-23 22.31214752398977 27.717334449210167 27.194267182759702 22.776250844040362 24-25 22.412956851705676 28.21424835233811 26.86188171071527 22.510913085240944 26-27 22.058697657609677 28.097747006628687 27.377816241714136 22.465739094047493 28-29 23.128607975349265 26.731590409799427 27.935596154029046 22.204205460822262 30-31 22.482857659131326 26.989795433147247 28.13436171528022 22.392985192441202 32-33 21.648327611294448 27.96507812722898 27.728271310235954 22.65832295124062 34-35 22.87706017175627 27.674062520803812 27.780578036881 21.668299270558922 36-37 22.448620528963662 27.542344672797647 26.667395790735053 23.341639007503638 38-39 21.93696563923575 27.247524940798296 27.763459471797162 23.05204994816879 40-41 21.638817297358987 25.655022872305018 29.03023328800084 23.675926542335162 42-43 22.54942985667957 25.099620538473978 29.09014826579425 23.260801339052204 44-45 20.84375505235428 25.5951078945116 29.53760853645779 24.023528516676336 46-47 21.643096938629945 24.661195066049128 29.677410151309097 24.018297844011833 48-49 20.6131299394193 24.58083291329447 30.248028987436875 24.558008159849358 50-51 19.241742669925536 25.55278699749879 30.843850155493634 24.361620177082045 52-53 19.20179935139659 25.738238119240314 31.623695898201596 23.436266631161494 54-55 17.932172441012277 26.53139830145793 30.34978934654633 25.18663991098346 56-57 18.673025896584846 26.100105564484682 29.53475544227715 25.69211309665332 58-59 18.334458720482363 26.11437103538788 30.302237776869013 25.248932467260744 60-61 19.051536391216274 26.794358481773482 28.624142882956566 25.529962244053678 62-63 18.10906428021189 27.932267544151635 27.497646197300973 26.461021978335502 64-65 19.02538302789375 28.302694271937916 26.0696725598912 26.60225014027713 66-67 18.650201143139736 27.748242969500424 25.71636439718875 27.885191490171092 68-69 18.498987151565874 28.19427669307364 25.309322960750936 27.997413194609557 70-71 19.600757020989263 27.662650144081258 24.982168161371007 27.754424673558475 72-73 20.225584646549184 27.357844582449665 24.68354430379747 27.73302646720368 74-75 20.86562877440584 27.74443884392624 24.53090376513329 26.85902861653463 76 20.99021388696041 27.520946466442858 24.995007085183882 26.49383256141285 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 2.5 15 3.5 16 5.0 17 6.0 18 13.5 19 15.5 20 28.0 21 46.0 22 44.0 23 68.0 24 113.5 25 133.0 26 184.0 27 319.0 28 467.5 29 532.0 30 658.0 31 913.0 32 1269.0 33 1496.0 34 1793.0 35 2368.5 36 2804.0 37 2961.0 38 3534.5 39 4513.5 40 5487.0 41 6615.5 42 7176.0 43 7163.5 44 7520.5 45 7718.5 46 7547.0 47 7762.5 48 7538.5 49 6844.5 50 6590.0 51 6184.5 52 5194.5 53 4243.5 54 3877.0 55 3549.5 56 2793.5 57 2113.0 58 1861.0 59 1535.0 60 1034.0 61 768.5 62 678.0 63 587.0 64 426.5 65 312.5 66 235.0 67 202.0 68 176.0 69 116.5 70 57.5 71 32.0 72 29.5 73 16.5 74 3.5 75 1.0 76 2.0 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 105149.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.05363817059601 #Duplication Level Percentage of deduplicated Percentage of total 1 58.196409915471556 23.309779455819836 2 16.179124323297557 12.96065583124899 3 8.846994016525786 10.630628917060553 4 5.16668249596353 8.277777249427004 5 3.0487225757431857 6.105621546567252 6 1.9493779086332985 4.684780644609079 7 1.2655522841675373 3.5482981293212488 8 0.9948713078165068 3.1878572311672007 9 0.7621806439357964 2.7475296959552633 >10 3.5544686105043217 22.109577837164405 >50 0.02849273435274005 0.7360982986048369 >100 0.004748789058790008 0.5953456523599844 >500 0.0 0.0 >1k 0.002374394529395004 1.106049510694348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 1163 1.106049510694348 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 474 0.4507888805409466 RNA PCR Primer, Index 24 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 152 0.14455677181903775 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.01046134532900931 0.0 2 0.0 0.0 0.0 0.011412376722555611 0.0 3 0.0 0.0 0.0 0.017118565083833418 0.0 4 0.0 0.0 0.0 0.023775784838657523 0.0 5 0.0 0.0 0.0 0.023775784838657523 0.0 6 0.0 0.0 0.0 0.023775784838657523 0.0 7 0.0 0.0 0.0 0.023775784838657523 0.0 8 0.0 0.0 0.0 0.024726816232203824 0.0 9 0.0 0.0 0.0 0.04374744410312984 0.0 10 0.0 0.0 0.0 0.04660053828376875 0.0 11 0.0 0.0 0.0 0.04850260107086135 0.0 12 0.0 0.0 0.0 0.05040466385795395 0.0 13 0.0 0.0 0.0 0.052306726645046556 0.0 14 0.0 0.0 0.0 0.06467013476114847 0.0 15 0.0 0.0 0.0 0.06942529172887997 0.0 16 0.0 0.0 0.0 0.06942529172887997 0.0 17 0.0 0.0 0.0 0.07418044869661147 0.0 18 0.0 0.0 0.0 0.08178869984498188 0.0 19 0.0 0.0 0.0 0.08559282541916709 0.0 20 0.0 0.0 0.0 0.0932010765675375 0.0 21 0.0 0.0 0.0 0.0941521079610838 0.0 22 0.0 0.0 0.0 0.0970052021417227 0.0 23 0.0 0.0 0.0 0.1017603591094542 0.0 24 0.0 0.0 0.0 0.11031964165137091 0.0 25 0.0 0.0 0.0 0.11031964165137091 0.0 26 0.0 0.0 0.0 0.12078098698038023 0.0 27 0.0 0.0 0.0 0.14550780321258405 0.0 28 0.0 0.0 0.0 0.17308771362542677 0.0 29 0.0 0.0 0.0 0.22444340887692701 0.0 30 0.0 0.0 0.0 0.2796032297026125 0.0 31 0.0 0.0 0.0 0.32049757962510345 0.0 32 0.0 0.0 0.0 0.35758780397340917 0.0 33 0.0 0.0 0.0 0.4089434992249094 0.0 34 0.0 0.0 0.0 0.5002425130053543 0.0 35 0.0 0.0 0.0 0.6219745313792808 0.0 36 0.0 0.0 0.0 0.8140828728756336 0.0 37 0.0 0.0 0.0 1.3057661033390713 0.0 38 0.0 0.0 0.0 1.8877973161894075 0.0 39 0.0 0.0 0.0 2.534498663800892 0.0 40 0.0 0.0 0.0 3.4465377702117945 0.0 41 0.0 0.0 0.0 4.528811496067485 0.0 42 0.0 0.0 0.0 5.867863698180677 0.0 43 0.0 0.0 0.0 7.304872133829138 0.0 44 0.0 0.0 0.0 8.457522182807255 0.0 45 0.0 0.0 0.0 9.681499586301344 0.0 46 0.0 0.0 0.0 10.867435734053581 0.0 47 0.0 0.0 0.0 11.914521298348058 0.0 48 0.0 0.0 0.0 12.902642916242666 0.0 49 0.0 0.0 0.0 13.963993951440337 0.0 50 0.0 0.0 0.0 14.941654224005934 0.0 51 0.0 0.0 0.0 15.953551626739198 0.0 52 0.0 0.0 0.0 17.116663021046325 0.0 53 0.0 0.0 0.0 18.25694966190834 0.0 54 0.0 0.0 0.0 19.393432177196168 0.0 55 0.0 0.0 0.0 20.378700700910137 0.0 56 0.0 0.0 0.0 21.348752722327365 0.0 57 0.0 0.0 0.0 22.148570124299802 0.0 58 0.0 0.0 0.0 22.975967436685085 0.0 59 0.0 0.0 0.0 23.747253896851134 0.0 60 0.0 0.0 0.0 24.512834168655907 0.0 61 0.0 0.0 0.0 25.26510000095103 0.0 62 0.0 0.0 0.0 26.11342000399433 0.0 63 0.0 0.0 0.0 26.906580186211947 0.0 64 0.0 0.0 0.0 27.770116691551987 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTGT 20 7.8952E-5 70.00001 34 GTCCGGA 15 0.0022192688 70.00001 33 CAATACG 20 7.8952E-5 70.00001 69 CAACGGA 25 2.3810209E-4 55.999996 64 AACGGAA 25 2.3810209E-4 55.999996 65 ACGGAAC 25 2.3810209E-4 55.999996 66 TCCGTAC 25 2.3810209E-4 55.999996 40 CATCGTT 25 2.3810209E-4 55.999996 32 ACCAACG 25 2.3810209E-4 55.999996 62 ATCATTG 25 2.3810209E-4 55.999996 22 TGCATAA 20 0.006934563 52.500004 30 CCGTACT 20 0.006934563 52.500004 41 TACGAGT 20 0.006934563 52.500004 4 TCAGATG 20 0.006934563 52.500004 3 CTCGCTA 20 0.006934563 52.500004 13 AACTCGT 20 0.006934563 52.500004 37 GGACCAG 20 0.006934563 52.500004 42 ACAAGTA 20 0.006934563 52.500004 30 CGGAACA 30 5.85492E-4 46.666668 67 ACCCTTT 30 5.85492E-4 46.666668 11 >>END_MODULE