FastQCFastQC Report
Wed 25 May 2016
SRR1780647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCACGTTAGTCATATCAAGTGAGTGGCTGAGGAGTTTTAGCGTTTGCG12.631578947368421No Hit
CGTGCACGCGGCGCAGCGCGGGCCACAGGACGGCGGCGACGGCGGCCCGA12.631578947368421No Hit
GTCCTGCACCTTCTCGATCGTCTCGACGAGCAGGGCGACCGCGCGCAGCG12.631578947368421No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC12.631578947368421No Hit
GGCTTGAACAGCTCGAGCTGGAGGATCTCTGCGCGCTTCTTCTCGCCGCC12.631578947368421No Hit
CAGGTAGAGCTCGTCGGTCAGGGACACGTCCGAGACGAGCCGGTTGTCCG12.631578947368421No Hit
CCCTTAGGATCAATTCAATTGGTCACGCGTGGGGCTGCTGTCTCTTATAC12.631578947368421No Hit
CCATGTGCCCCGGCCCTCTCGCGGTGATCTTCTGGGGTCGTTGGCGCAGG12.631578947368421No Hit
AACCTAGATAGTACAGCCTGCCACACACCAAGACTCTGTGGTATATAGCC12.631578947368421No Hit
CGGCAACGGCCCCCCCACCCGGCGCCCGCCAGCCACCCGCGCCCGCCCAA12.631578947368421No Hit
GTTCGGTGCGCCGTCCGCCAATTTGCCCTGCGACCTCGGAACCCTCTCCT12.631578947368421No Hit
CTTATACACATCTGACGCTGCCGACGAATCTGGCGGTGTAGGTTTTGGGG12.631578947368421No Hit
GTCCCCAGCAGCCAGGACTTCGACGGGCAGACCATCCGGGACTTCTCCCC12.631578947368421No Hit
TTTACGCATGGGTGACGATCAGGACGCGTGCCGGTCTGGACCCTGTCTCT12.631578947368421No Hit
GGGTCGCCTTCCCAGAGGCTTTGGTGGGTTCGAGGCCTTCCCTCTCGGTG12.631578947368421No Hit
CGCTGATGGAGGCCGAGCTGGACCGGATGGCCGAGGCCGACGTCACCGGG12.631578947368421No Hit
GTGGTGGCCCCGGACGCCCACGCGCGCGTGCTGCTCACGGCCTCCGAGGC12.631578947368421No Hit
TTGGGTTGTGACAGCGTGAAGTTGCTAGCGCGTCGAGACAGTCGGTAACA12.631578947368421No Hit
CATATACACGGGGTCATGATAGCACTAGTCATAATGCCAATTAATATTTG12.631578947368421No Hit
ACCCTCACCGTGGCCTCCGGCGTGGACGTCAACTTCCTCGCGGCCACCCG12.631578947368421No Hit
ATGATGGACAATGGGGCGCATGCTCCCCCTCGTCGCACCCCTGACCCTGT12.631578947368421No Hit
GAAGAATGACTCATGAATGGTGTTTCAGACAGCTGGCCTGGCTGGGGGTG12.631578947368421No Hit
GAGCTCAAGGCCGTCGACAAGATCATCGTCCTGGCGCTGTCTCTTATACA12.631578947368421No Hit
GGCGTGGACCGGGCGCAGATCTCGATCACACCGTCCTCCCGGTGTGGATC12.631578947368421No Hit
ATACTCATGTCTCATTATGTGTTCTCCAAGAAGCAGACGTTAGACAAAGA12.631578947368421No Hit
CTCAAGGGCGACACGGTCGCGGACCCGGATCGGGCGCTCAGGGAGTCTGT12.631578947368421No Hit
GCCTCGCCGGGTGGGCTCCTTTAGGTGATCTTAGGCAGCTTGGGGGGGTT12.631578947368421No Hit
GTCCTGGACCGGGCGGCCTTCGACCGGGTCACGGCCTGTCTCTTATACAC12.631578947368421No Hit
CTCTACCTCGACGCCGGCCCCCGCGAGAGCCGGGATCTGTCTCTTATACA12.631578947368421No Hit
CACTCCCTCTTAGCCAATATTGTGCCTATTGCCCTACTCGTCTTTGCCGC12.631578947368421No Hit
ATGTATGCACTAAGGCCTTGGGCTTCTCAACCAAAGGGCACCACAGGACT12.631578947368421No Hit
CCTTCACCGAGGCGGACATCGAGGCGTTCGAGTCCGTGCTCACGCTCGTA12.631578947368421No Hit
GCCCGCGCTCACGAGCCTGCGCCGCGACCTCCTTGGCCAGCAGCGCACCG12.631578947368421No Hit
ACTCCTTGGCGCCTGCCTGAGCCTCCAAATCACCACAGGACTATTCCTAG12.631578947368421No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATC12.631578947368421No Hit
GTTTCTATTGATGCTGATCTTATTGGTTTCTTTTTGTGGGTTTTCATTCT12.631578947368421No Hit
AGTCCGTCGACGTGACCGCGAGCCCCGCGGCGGCCAGTTTGCGGTCGAGC12.631578947368421No Hit
CTTATACACATCTGACGCTGCCGACGAACTTCTAGGTGTAGATCTCGGTG12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers