FastQCFastQC Report
Wed 25 May 2016
SRR1780647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTGTGTCCACCCCCAGCCAGGCCAGCTGTCTGAAACACCATTCATGA12.631578947368421No Hit
GCCGTGACCCGGTCGAAGGCCGCCCGGTCCAGGACCTGTCTCTTATACAC12.631578947368421No Hit
CTTATACACATCTCCGGCCCACGAGACTTTAATGCATCTCGTATGCCGTC12.631578947368421No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG12.631578947368421No Hit
TTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAAACCTGTCTCTT12.631578947368421No Hit
CTTATACAAATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT12.631578947368421No Hit
ATCCCGGCTCTCGCGGGGGCCGGCGTCGAGGTAGAGCTGTCTCTTATACA12.631578947368421No Hit
CTGCATTGCTGCGTGCTTGATGCTTGTCCCTTTTGATCGTGGTGATTTAG12.631578947368421No Hit
CGCCAGGACGATGATCTTGTCGACGGCCTTGAGCTCCTGTCTCTTATACA12.631578947368421No Hit
ATCGAGCGGGCCAGCGAGATGGCGGTGTCCTCGTTCAGCGTCCCCGAGCG12.631578947368421No Hit
CTATACACATCTCCGAGCCCAAGAGACACCGGCTAATCTCGTATGCCGTC12.631578947368421No Hit
ACTCCCTGAGCGCCCGATCCGGGTCCGCGACCGTGTCGCCCTTGAGCTGT12.631578947368421No Hit
CCCCATCATACTCTTTCACCCACAGCACCAATCCTACCCTGTCTCTTATA12.631578947368421No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT12.631578947368421No Hit
TTCATGAACCGCAACGTCAACGAGGGCTTCTCCGGCGGCGAGAAGAAGCG12.631578947368421No Hit
GAGTTGGCTTGTGAGCCCCGCCCCCTCCGAGCCCACGAGACTTTAATGCA12.631578947368421No Hit
ATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTTCTGCTT12.631578947368421RNA PCR Primer, Index 36 (100% over 28bp)
CGTGGGGCCTGGGCCTGGTGGTCGGTGCGCTGCTGGCCAAGGAGGTCGCG12.631578947368421No Hit
GTGCGGGAGCGGCCGCGGAAGGCGGCCACCGGGGTGCCGTCGTCGGTGGT12.631578947368421No Hit
GAGCTGGAGCGCACCGGCCGGATCGATCGGGCCGCCGTCGCCGCCGTCCT12.631578947368421No Hit
CTGTCTCTTATACACATCTCAGAGCCCACGAGACGATCCAAAATCTCGTA12.631578947368421No Hit
ACCTGGGCGAGACCGCCGCGTGGGTGCGGGACCGGCAGATCGCCCTGGAG12.631578947368421No Hit
GCGGCGGGCGGTGCGCACGGCCTCGGAGGCCGTGAGCAGCACGCGCGCGT12.631578947368421No Hit
ATACACATCTCCGAGCCGACGAGACCGGTTCCCATCTCGTATGCCGTCTT12.631578947368421RNA PCR Primer, Index 27 (95% over 22bp)
CACTGGGGCAGCCCTCAACAAATTAAGGAGAGAAATTGATATATAAATAC12.631578947368421No Hit
CAGCTGGCTAATGTAGCAAAACTAACCTCAAATATTAATTGGCATTATGA12.631578947368421No Hit
CCCCGGTGACGTCGGCCTCGGCCATCCGGTCCAGCTCGGCCTCCATCAGC12.631578947368421No Hit
GGCACACATGTACAGTCAGGGACCACATAATCATATTTTGGTCAATGACA12.631578947368421No Hit
GTCGTCGGCCGATCCACACCGGGAGGACGGTGTGATCGAGATCTGCGCCC12.631578947368421No Hit
AAAAAAAAAAAAGCTAAACTAACCAAGATATTTTGTACTCTGTGGCAAGC12.631578947368421No Hit
GCGTGATCATCCCGCTGACCGCCGAGTTCTTCGCGCTGCGCGCGGTCGCC12.631578947368421No Hit
GGTCCAGACCGGGCCGCGTCCAGATCGTCCCCCATGCGTAGCCTGTCTCT12.631578947368421No Hit
GTCCAGCGCATCTCCCCGGGCGGGGCGGCGCTCGACCGCAAACTGGCCGC12.631578947368421No Hit
GTCGAACGTCGGGTGCGGGGAGAAGTCCCGGATGGTCTGCCCGTCGAAGT12.631578947368421No Hit
CACCGGCCCTGCGCCTCATGCCGGCGCCGCCGCCCAGCCCCCGCGCCGCT12.631578947368421No Hit
GGCCCAGGTGGGGCGTGAGCTCGGCCGGTCGCTGGGCCTGTCTCTTATAC12.631578947368421No Hit
GGAGAAGACCTGCGCCAACGACCCCAGAAGATCACCGCGAGAGGGCCGGG12.631578947368421No Hit
GGTCAGGGGTGCGACGAGGGGGAGCATGCGCCCCATTGTCCATCATCTGT12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)