FastQCFastQC Report
Wed 25 May 2016
SRR1780646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70700
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACC252.3775667E-455.99094442
TGTCAGT200.006929645352.49151219
ATCTATA200.006929645352.49151223
GCGACAA200.006929645352.49151216
TGCTTCG200.006929645352.49151252
ACGCACT200.006929645352.4915128
TAGGTTG200.006929645352.49151232
GCTGCTT200.006929645352.49151250
TTCCGAC200.006929645352.49151224
TGACGCA200.006929645352.4915126
CCATGGA200.006929645352.49151230
GCTTCGC200.006929645352.49151253
TTAGTCC200.006929645352.4915123
TAGATGA200.006929645352.49151210
GACGCAC200.006929645352.4915127
GAACTCT305.8464945E-446.65912222
TTGTCAC305.8464945E-446.65912241
CCTGAAC305.8464945E-446.65912219
ACATATG400.002275245535.3948481
GTCACCA400.002406067734.99434343
Produced by FastQC (version 0.11.5)