##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780646_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70700 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.492927864214995 34.0 27.0 34.0 12.0 34.0 2 30.801994342291373 34.0 32.0 34.0 21.0 34.0 3 31.06947666195191 34.0 32.0 34.0 23.0 34.0 4 31.462517680339463 34.0 34.0 34.0 23.0 34.0 5 31.45813295615276 34.0 34.0 34.0 23.0 34.0 6 34.27128712871287 38.0 35.0 38.0 23.0 38.0 7 33.55476661951909 37.0 34.0 38.0 21.0 38.0 8 32.773663366336635 37.0 32.0 38.0 11.0 38.0 9 33.26063649222065 37.0 34.0 38.0 21.0 38.0 10-11 33.63733380480905 37.0 34.0 38.0 21.0 38.0 12-13 33.94183168316832 38.0 34.0 38.0 22.0 38.0 14-15 33.51668316831683 37.0 34.0 38.0 21.0 38.0 16-17 33.34804101838755 37.0 34.0 38.0 21.0 38.0 18-19 33.36395332390382 37.0 34.0 38.0 20.0 38.0 20-21 33.46522630834512 37.0 34.0 38.0 21.0 38.0 22-23 33.42899575671853 37.0 34.0 38.0 21.0 38.0 24-25 33.80205091937765 37.5 34.0 38.0 21.0 38.0 26-27 33.856463932107495 38.0 34.0 38.0 21.0 38.0 28-29 34.00036775106082 38.0 34.0 38.0 22.0 38.0 30-31 33.814101838755306 38.0 34.0 38.0 21.0 38.0 32-33 33.598748231966056 37.0 34.0 38.0 21.0 38.0 34-35 33.480304101838755 37.0 34.0 38.0 21.0 38.0 36-37 33.43557991513437 37.0 34.0 38.0 21.0 38.0 38-39 33.93501414427157 37.5 34.0 38.0 21.5 38.0 40-41 33.47461810466761 37.5 34.0 38.0 21.0 38.0 42-43 33.79350070721358 37.0 34.0 38.0 21.5 38.0 44-45 32.89100424328147 37.0 32.5 38.0 16.0 38.0 46-47 33.577128712871286 37.0 34.0 38.0 21.0 38.0 48-49 33.32404526166903 37.0 34.0 38.0 21.0 38.0 50-51 33.31186704384724 37.0 34.0 38.0 21.0 38.0 52-53 33.27387553041018 37.0 34.0 38.0 21.0 38.0 54-55 32.97323903818953 37.0 32.5 38.0 21.0 38.0 56-57 33.058224893917966 37.0 33.0 38.0 16.0 38.0 58-59 32.92327439886846 37.0 32.0 38.0 16.0 38.0 60-61 32.9632531824611 37.0 32.5 38.0 19.5 38.0 62-63 32.965629420084866 37.0 32.0 38.0 19.5 38.0 64-65 32.995261669024046 37.0 32.5 38.0 19.0 38.0 66-67 32.9619801980198 37.0 32.5 38.0 19.5 38.0 68-69 32.5461315417256 37.0 31.0 38.0 15.0 38.0 70-71 32.37744695898161 37.0 31.0 38.0 11.0 38.0 72-73 32.45682461103253 37.0 31.0 38.0 11.0 38.0 74-75 32.15683168316832 37.0 30.5 38.0 11.0 38.0 76 31.526930693069307 36.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.462534489759882 1101 2 -1.0981961286765696 1101 3 -1.7346547555737963 1101 4 -2.4780188477941927 1101 5 -0.8828411011664201 1101 6 -2.180357097417847 1101 7 -2.266131649362709 1101 8 -1.2299960967189847 1101 9 -0.7616574052821221 1101 10-11 -2.141614744606713 1101 12-13 -2.460642450757888 1101 14-15 -1.684978197760607 1101 16-17 -2.6277892256637045 1101 18-19 -0.7680784372876772 1101 20-21 -1.4577322685674652 1101 22-23 -0.6201440607491904 1101 24-25 -1.9771050265857042 1101 26-27 -2.0133610880402912 1101 28-29 -0.5840948485138426 1101 30-31 -1.842418826831377 1101 32-33 -1.452202242732156 1101 34-35 -2.1311232731721645 1101 36-37 -0.9876592892078477 1101 38-39 -1.4141071480974077 1101 40-41 -0.21755310052122212 1101 42-43 -1.1944520735795834 1101 44-45 -0.9270100508070769 1101 46-47 -0.267093480307544 1101 48-49 -1.1336840029625321 1101 50-51 -1.1453682974713715 1101 52-53 -0.8872786044501737 1101 54-55 -1.429524301850428 1101 56-57 -1.498387574519466 1101 58-59 -1.1214603791058195 1101 60-61 -0.5546971961437421 1101 62-63 -1.7379733934245998 1101 64-65 -1.0019253497572116 1101 66-67 -1.5961184959497636 1101 68-69 -0.9904947061114946 1101 70-71 0.06889824499709363 1101 72-73 -1.3718923649463548 1101 74-75 -2.033500125130061 1101 76 -2.5755673068942926 1102 1 -0.6386982136764132 1102 2 -1.1521688413571454 1102 3 -0.5127446432142442 1102 4 -0.24273259846300022 1102 5 -0.4205618074264521 1102 6 -0.5862559738223396 1102 7 0.5833868097063117 1102 8 1.474525037577429 1102 9 0.004393959083849097 1102 10-11 -1.1567966600695243 1102 12-13 -4.691748657285444 1102 14-15 -1.2711539602004152 1102 16-17 -2.7442619383442803 1102 18-19 -1.5158044940025022 1102 20-21 -0.6868654948916983 1102 22-23 -1.2306978328198142 1102 24-25 -0.6638693390522548 1102 26-27 -5.416885700132319 1102 28-29 -1.3735210122377524 1102 30-31 -1.0058417802824167 1102 32-33 -6.521055907793688 1102 34-35 -1.041770678201594 1102 36-37 -0.40750278840528154 1102 38-39 -0.7685747778459344 1102 40-41 -0.5723551336940389 1102 42-43 -0.37543120680590647 1102 44-45 -6.364576931491932 1102 46-47 -1.1797138655402932 1102 48-49 -0.3905058328180715 1102 50-51 -6.5018423477656455 1102 52-53 -2.3830651213041065 1102 54-55 -0.6688568326155462 1102 56-57 -2.066839955471849 1102 58-59 -0.472483653584149 1102 60-61 -0.5529582983374297 1102 62-63 -5.167180188502179 1102 64-65 -1.4747717917047787 1102 66-67 -1.3139488865329625 1102 68-69 -7.454981062451786 1102 70-71 -0.8749888604068659 1102 72-73 -0.731911091538116 1102 74-75 -6.708660638773722 1102 76 -3.2674479917525048 1103 1 -0.09923399714904235 1103 2 -0.39930450306080445 1103 3 -0.13243800680532303 1103 4 -0.25839651610289494 1103 5 -0.5872745987033667 1103 6 -1.797967934856274 1103 7 0.3434742619673443 1103 8 0.9493963499148421 1103 9 -0.22101701119345307 1103 10-11 0.41277770202711395 1103 12-13 -1.2979782471454087 1103 14-15 -0.7979708085980448 1103 16-17 -0.7574074522646939 1103 18-19 -0.8719372221809465 1103 20-21 -0.7434465542817463 1103 22-23 -0.25499627749795906 1103 24-25 0.39307149229771454 1103 26-27 -0.7197445034754324 1103 28-29 1.0264347081364065 1103 30-31 0.25210087760211763 1103 32-33 -5.2484871359998095 1103 34-35 -1.3101052107747932 1103 36-37 0.0021806122699814523 1103 38-39 0.7135611606053871 1103 40-41 0.47435987156416815 1103 42-43 0.593725266321897 1103 44-45 -4.672597078721687 1103 46-47 0.019544450726932894 1103 48-49 -0.010531293602923597 1103 50-51 -0.6667147670937013 1103 52-53 -0.5430667817900741 1103 54-55 -0.039643349469479006 1103 56-57 0.8518792563508129 1103 58-59 -0.02139880275113626 1103 60-61 0.6747773522963243 1103 62-63 0.5851998413865616 1103 64-65 0.8153981313593732 1103 66-67 -0.7658639803504173 1103 68-69 -3.611800124830708 1103 70-71 0.12164865550612802 1103 72-73 1.2141092770897686 1103 74-75 -1.782884361583264 1103 76 -0.09055088653304466 1104 1 -2.0555404421408348 1104 2 0.00150625227581358 1104 3 0.6872202444262037 1104 4 0.7757013903010446 1104 5 0.16552199407167478 1104 6 -0.2688987640845113 1104 7 1.5344635887325282 1104 8 2.257355093757205 1104 9 0.6490568804321626 1104 10-11 0.5912721601551922 1104 12-13 0.604833739718309 1104 14-15 0.3086973974774878 1104 16-17 0.33128815528868216 1104 18-19 1.5994810865218412 1104 20-21 1.7465088028611078 1104 22-23 0.7343946297269994 1104 24-25 1.240156878176208 1104 26-27 1.4434841500549425 1104 28-29 0.6078694372004492 1104 30-31 -0.33832656492661073 1104 32-33 -0.2684224808273967 1104 34-35 0.5307070839706967 1104 36-37 1.4271472584112033 1104 38-39 0.9899107090454464 1104 40-41 1.193533209002588 1104 42-43 1.4112026883251758 1104 44-45 -1.6141380269975514 1104 46-47 0.6413886625495948 1104 48-49 1.4954082589422342 1104 50-51 1.9781436072905336 1104 52-53 0.4642838955498263 1104 54-55 0.5332733171971924 1104 56-57 1.0243058778614795 1104 58-59 1.82980450184656 1104 60-61 1.339276018141966 1104 62-63 1.874749820861112 1104 64-65 0.47203298357612056 1104 66-67 1.0400422183359552 1104 68-69 -3.125539348968637 1104 70-71 0.3479161021399477 1104 72-73 1.526545135053638 1104 74-75 -0.33260726798720697 1104 76 -0.08896016403715024 1105 1 -1.85963491408803 1105 2 -0.7688114044898633 1105 3 -0.8262389139896378 1105 4 -0.5988345007880653 1105 5 -0.6621904646742003 1105 6 -0.9142679885069569 1105 7 -0.023910998726215382 1105 8 -0.532094163668539 1105 9 -0.32000252550842845 1105 10-11 -0.4831399685576798 1105 12-13 -2.8817586695226183 1105 14-15 -1.4055404325555116 1105 16-17 -0.5613995509819532 1105 18-19 -0.5924537319599992 1105 20-21 -1.5867987466438436 1105 22-23 -1.016714074893457 1105 24-25 -1.1727969172033355 1105 26-27 -1.249069480308087 1105 28-29 -2.1665736321064806 1105 30-31 -1.4107533388540077 1105 32-33 -0.3951242135006652 1105 34-35 0.05440242225451897 1105 36-37 -0.6712696145260963 1105 38-39 -0.8424428388093403 1105 40-41 -0.8597619642647416 1105 42-43 -0.5455836153051763 1105 44-45 -0.03500745769062519 1105 46-47 -0.31916208002466107 1105 48-49 -1.2100630694406078 1105 50-51 -1.0425865907292717 1105 52-53 -1.8786152381135395 1105 54-55 -1.8020195870932412 1105 56-57 -1.0247607475510563 1105 58-59 -1.0142291202656502 1105 60-61 -1.2777052112309661 1105 62-63 -0.8927058309540641 1105 64-65 -1.195819739196157 1105 66-67 -0.1338954879346801 1105 68-69 0.17729171538780264 1105 70-71 0.2647374717769111 1105 72-73 -0.44161183689355 1105 74-75 -1.4795750897693836 1105 76 -2.183122705291275 1106 1 -0.7999247531277973 1106 2 -0.12203890601096745 1106 3 -0.24722458797603153 1106 4 -0.054169419754821035 1106 5 0.19936161232120853 1106 6 -0.24390458345136778 1106 7 -0.13744050810173292 1106 8 0.12109461889686912 1106 9 1.4165149809908186 1106 10-11 -0.044813733699562874 1106 12-13 0.1899128831075032 1106 14-15 -0.6499123557813533 1106 16-17 -0.07446719852882921 1106 18-19 -1.0566525261411002 1106 20-21 -0.1516668256305138 1106 22-23 -0.7706827678521435 1106 24-25 0.07279188748718468 1106 26-27 -0.3743114737811766 1106 28-29 -1.6450984398945252 1106 30-31 -0.9016267278688872 1106 32-33 0.14315223984299053 1106 34-35 0.08728587819787492 1106 36-37 0.094992346865574 1106 38-39 -0.6105024660011011 1106 40-41 -0.6335879502152935 1106 42-43 -0.0871096952874808 1106 44-45 0.17396427747172538 1106 46-47 -0.1678815888470524 1106 48-49 -0.12969357997769038 1106 50-51 -0.008762817290474345 1106 52-53 -0.2818316770758713 1106 54-55 -0.16254837740243744 1106 56-57 0.17617680430468852 1106 58-59 -0.4333618156580954 1106 60-61 0.27737250324438634 1106 62-63 0.1361121346413725 1106 64-65 0.38867055340852374 1106 66-67 0.503874853345259 1106 68-69 0.6368995476794055 1106 70-71 -0.7487726781207549 1106 72-73 -0.21570055861488413 1106 74-75 -1.827893580836367 1106 76 -0.2457921007224364 1107 1 0.47233945981994907 1107 2 0.7116778209032617 1107 3 0.25063936207326165 1107 4 0.6228214883402572 1107 5 0.3986715038755939 1107 6 0.6162115300331337 1107 7 0.7758851573599799 1107 8 1.5585193094434828 1107 9 -4.52923489405066E-4 1107 10-11 0.22393147388068257 1107 12-13 0.6403729554045796 1107 14-15 0.48991676022259156 1107 16-17 1.3205651160729914 1107 18-19 1.4910252041689063 1107 20-21 1.081833557763069 1107 22-23 0.016410561099554855 1107 24-25 0.6470196232742467 1107 26-27 0.9195870912314206 1107 28-29 -0.32901781770151217 1107 30-31 0.455025150759667 1107 32-33 1.7521044799569196 1107 34-35 1.4269999761275542 1107 36-37 -0.2844029866868354 1107 38-39 1.1513690423787821 1107 40-41 1.0503040423359167 1107 42-43 0.48657033538400185 1107 44-45 0.421554374963236 1107 46-47 1.0534597409809692 1107 48-49 0.9983187981579178 1107 50-51 1.293707332780727 1107 52-53 1.2818390426086523 1107 54-55 0.7985919446481731 1107 56-57 1.1145325935477572 1107 58-59 1.630815531258321 1107 60-61 0.35321596912236686 1107 62-63 -0.40726611051143635 1107 64-65 0.6059177874976882 1107 66-67 1.6500299634339939 1107 68-69 1.5259618765215563 1107 70-71 0.4652567884144645 1107 72-73 1.1477154781908965 1107 74-75 0.3432505751500443 1107 76 0.020721208511869094 1108 1 -0.6929856175794313 1108 2 -0.4536472564961187 1108 3 -0.6267600187316908 1108 4 -0.6267657149621151 1108 5 -0.2226907252265704 1108 6 -0.13693604478626753 1108 7 0.6974021852237584 1108 8 -0.23688832729542497 1108 9 -1.7627852248309956 1108 10-11 -0.46974757668691325 1108 12-13 -0.06906976905361972 1108 14-15 0.08372480975818775 1108 16-17 -0.8904771955885096 1108 18-19 -0.3170759310220106 1108 20-21 0.21632788180847484 1108 22-23 -0.6037906772905401 1108 24-25 -0.7620361042799431 1108 26-27 -1.2145202359099656 1108 28-29 0.02727733193729165 1108 30-31 -0.2750316087862643 1108 32-33 -0.2646395860905528 1108 34-35 -0.7380155037486062 1108 36-37 -0.20824199597475967 1108 38-39 0.3684743055366795 1108 40-41 0.022233866897330756 1108 42-43 0.4367509339392157 1108 44-45 -0.5341215796291294 1108 46-47 0.1593162941285371 1108 48-49 -0.48405994281215214 1108 50-51 -0.6063958663936759 1108 52-53 -0.33596281497649017 1108 54-55 -0.7264854547326323 1108 56-57 0.7700021497913099 1108 58-59 -1.6775694016622111 1108 60-61 0.29204465849284844 1108 62-63 -0.4383806615950263 1108 64-65 -0.6528025427396713 1108 66-67 -0.10507839569913813 1108 68-69 0.6548060457682041 1108 70-71 0.9677077688066191 1108 72-73 -0.33548885617442536 1108 74-75 0.4618006267496355 1108 76 0.1721143973663608 1109 1 0.36874629841745943 1109 2 -0.04955080847952331 1109 3 0.7345570670863033 1109 4 0.4017020060645926 1109 5 0.9890800810995906 1109 6 1.014839946917128 1109 7 -0.14913657547108272 1109 8 1.8853569410478457 1109 9 1.532677570087337 1109 10-11 1.747753071485242 1109 12-13 1.3867508168940006 1109 14-15 0.9778912603674783 1109 16-17 -0.2845010483237971 1109 18-19 0.6403780487550179 1109 20-21 1.6407898989202252 1109 22-23 1.8706194860488452 1109 24-25 1.8413473543666754 1109 26-27 1.038876186179742 1109 28-29 0.9550061367078371 1109 30-31 1.4319038332671425 1109 32-33 1.815552272867187 1109 34-35 1.0248701586833349 1109 36-37 1.1942988388709708 1109 38-39 1.5017385005068604 1109 40-41 0.937414058756282 1109 42-43 1.1060405372578543 1109 44-45 1.1599152365492458 1109 46-47 0.4850616921062496 1109 48-49 0.8047396423576672 1109 50-51 2.2175216861082703 1109 52-53 1.6145686861902249 1109 54-55 0.9578935963433324 1109 56-57 1.5563127538877524 1109 58-59 1.3726898671995968 1109 60-61 1.132905431114061 1109 62-63 0.12953481675602774 1109 64-65 1.5124424663347398 1109 66-67 0.9385695171865223 1109 68-69 2.171451106696388 1109 70-71 1.3039146653583416 1109 72-73 0.7017940061538042 1109 74-75 2.5668693329980172 1109 76 1.2197939410531617 1110 1 0.6625552446263363 1110 2 0.7687277765639209 1110 3 -0.2962125445185215 1110 4 0.22866935512516662 1110 5 0.3849263073045037 1110 6 0.3680097367530095 1110 7 1.1432366205583264 1110 8 2.0396258224004242 1110 9 -0.8993099537386939 1110 10-11 -0.22994329210611397 1110 12-13 0.8442600378757561 1110 14-15 0.735113331313336 1110 16-17 0.8148256343506475 1110 18-19 1.0241565471583627 1110 20-21 -0.4365549534203055 1110 22-23 0.47883296724794633 1110 24-25 1.065324382363812 1110 26-27 -0.6704710425748672 1110 28-29 0.7050742110697996 1110 30-31 0.04563752636316565 1110 32-33 1.641046740278128 1110 34-35 1.3432725152939753 1110 36-37 -0.0362504593442452 1110 38-39 0.48114291172552015 1110 40-41 0.6981264878970634 1110 42-43 0.6838680987104411 1110 44-45 1.310681382550186 1110 46-47 0.3277108987305013 1110 48-49 1.063731646714423 1110 50-51 0.5307503912101339 1110 52-53 0.4186259181628884 1110 54-55 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1.193239073960548 2116 50-51 1.1659870138839423 2116 52-53 1.6388202966487313 2116 54-55 0.649917090090959 2116 56-57 0.7298633412314501 2116 58-59 0.19790775276231898 2116 60-61 -0.5830854745320195 2116 62-63 1.3611566432054403 2116 64-65 1.8396497418178797 2116 66-67 0.8567203318890648 2116 68-69 1.3487910081742172 2116 70-71 -2.3344900333911838 2116 72-73 1.241202873148879 2116 74-75 -0.33981880644874707 2116 76 -0.006428112755099136 2117 1 -0.581121703650961 2117 2 0.24108958560914573 2117 3 0.5291573991223366 2117 4 0.02388854499717752 2117 5 -0.6476398383408508 2117 6 -0.6478985338819356 2117 7 -1.5552003155031962 2117 8 -2.988414933867105 2117 9 0.1939463831945929 2117 10-11 -0.14500201111552968 2117 12-13 0.3245621006759478 2117 14-15 -0.3258128465372394 2117 16-17 0.14347162674355474 2117 18-19 -0.1563315285794431 2117 20-21 -0.3227677855366764 2117 22-23 -0.38800120360632917 2117 24-25 -0.638526363076096 2117 26-27 0.8806963968321071 2117 28-29 0.25997579079161426 2117 30-31 -0.9148833098621623 2117 32-33 0.06004782304000855 2117 34-35 -0.2307023313147596 2117 36-37 -1.0492713649773862 2117 38-39 0.17277788215778855 2117 40-41 -0.9004326059145384 2117 42-43 -0.45096351793633005 2117 44-45 -0.02371157672131119 2117 46-47 -0.7302446247432215 2117 48-49 -0.2741733478717663 2117 50-51 0.3146422260750654 2117 52-53 0.6741854839476176 2117 54-55 0.007317976497368761 2117 56-57 -1.0171161985657733 2117 58-59 -0.8232658250410978 2117 60-61 0.3303988312174724 2117 62-63 -0.6098985447005774 2117 64-65 -0.23074671255647417 2117 66-67 -0.8875878461207662 2117 68-69 0.29275312340705284 2117 70-71 1.1293099787513725 2117 72-73 0.7277853514440693 2117 74-75 -0.43533516853969445 2117 76 -0.4131429448842958 2118 1 0.8827036054782127 2118 2 1.0664864110059717 2118 3 0.465080694161653 2118 4 0.7693356495603041 2118 5 -0.12622734455266382 2118 6 0.9962302041694571 2118 7 1.1320164987854398 2118 8 -3.2233823401052284 2118 9 -0.7180066116313313 2118 10-11 -0.9266676546596244 2118 12-13 0.7685242159087764 2118 14-15 -0.3043565046389105 2118 16-17 -0.2504744108488879 2118 18-19 -0.1265440457532847 2118 20-21 -2.3889491997843955 2118 22-23 0.2575014418963164 2118 24-25 0.23288174590106792 2118 26-27 1.1404087532295506 2118 28-29 -0.0731821501011396 2118 30-31 5.04453591908316E-4 2118 32-33 0.4542131011831856 2118 34-35 1.202210060161164 2118 36-37 0.04441743036887402 2118 38-39 -0.4101388941291546 2118 40-41 -0.5568388148069374 2118 42-43 0.37168549255798666 2118 44-45 0.6507015894045622 2118 46-47 0.15784038212631657 2118 48-49 -0.42733479661333007 2118 50-51 0.07420842210534317 2118 52-53 -0.3813262234977941 2118 54-55 0.7275524177262938 2118 56-57 1.3405674519355557 2118 58-59 1.017097821952376 2118 60-61 -2.2570781485246947 2118 62-63 0.49516152721032114 2118 64-65 -0.20166079949266447 2118 66-67 -1.6668856917169599 2118 68-69 1.748261907645123 2118 70-71 -0.6605991095002608 2118 72-73 -1.4598553279093203 2118 74-75 0.8084310918011539 2118 76 0.43435332802634363 2119 1 -0.48694934044591776 2119 2 -0.572469736424754 2119 3 0.06619270205332128 2119 4 -0.8345563780605367 2119 5 -0.3412753223125087 2119 6 0.08447341105672734 2119 7 -3.1791663547702953 2119 8 -0.06070634692076027 2119 9 -1.6440222156130346 2119 10-11 -0.3321918228956591 2119 12-13 -0.6229204975698295 2119 14-15 -0.6819011194716609 2119 16-17 -0.932924415332792 2119 18-19 -0.6439864276024494 2119 20-21 -1.9258517197377643 2119 22-23 -0.020769565188253125 2119 24-25 -0.7534871896204081 2119 26-27 -0.42604268610324425 2119 28-29 -0.41243423511593846 2119 30-31 -0.4247937259435588 2119 32-33 -0.31141330003643475 2119 34-35 0.050878341032841945 2119 36-37 -1.3507040833967068 2119 38-39 -0.5950998841748927 2119 40-41 -0.504780703426789 2119 42-43 -1.025767650657869 2119 44-45 -1.1097683236077245 2119 46-47 -1.2764256029871248 2119 48-49 -1.3774289585718904 2119 50-51 0.3187156411888452 2119 52-53 -1.4741430112298346 2119 54-55 -1.5339936319553544 2119 56-57 -0.027291932872984148 2119 58-59 1.0868225103211415 2119 60-61 -0.028264589202663615 2119 62-63 -0.6829390915682652 2119 64-65 0.22547578018629366 2119 66-67 -0.20316046527986487 2119 68-69 -0.7117416794771145 2119 70-71 -0.7992413837357546 2119 72-73 -1.97429349488986 2119 74-75 -1.0457075504730824 2119 76 -0.6339813504778284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 9.0 13 106.0 14 313.0 15 511.0 16 742.0 17 848.0 18 900.0 19 895.0 20 807.0 21 721.0 22 644.0 23 727.0 24 727.0 25 806.0 26 966.0 27 1180.0 28 1308.0 29 1657.0 30 2040.0 31 2519.0 32 3247.0 33 4180.0 34 5668.0 35 8327.0 36 14131.0 37 16720.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.452931285091346 23.1709750049876 13.42377518739134 28.952318522529712 2 16.663366336633665 20.31824611032532 38.64639321074964 24.371994342291373 3 16.4016973125884 25.2970297029703 29.27015558698727 29.031117397454032 4 12.558698727015557 20.397454031117398 37.895332390381895 29.148514851485146 5 11.78783592644979 38.330975954738335 35.445544554455445 14.435643564356434 6 27.12305516265912 38.89108910891089 17.554455445544555 16.43140028288543 7 24.338048090523337 30.751060820367755 24.596888260254595 20.314002828854314 8 21.39179632248939 37.68882602545969 19.343705799151344 21.575671852899575 9 22.882602545968883 16.933521923620933 22.00848656294201 38.17538896746818 10-11 24.285714285714285 26.202263083451204 27.065063649222065 22.446958981612447 12-13 23.213578500707214 23.707213578500706 26.075671852899575 27.003536067892504 14-15 20.992220650636494 25.535360678925034 24.150636492220652 29.32178217821782 16-17 22.223479490806223 28.046676096181045 25.293493635077795 24.436350777934937 18-19 21.231966053748234 26.884724186704386 26.07920792079208 25.804101838755304 20-21 21.004243281471005 27.06930693069307 26.24045261669024 25.685997171145686 22-23 22.355021216407355 26.88684582743989 26.219943422913722 24.538189533239038 24-25 21.835219236209337 28.004243281471002 25.66760961810467 24.49292786421499 26-27 20.647100424328148 28.187411598302685 26.700848656294202 24.464639321074966 28-29 21.804809052333805 27.184582743988685 27.847949080622346 23.162659123055164 30-31 21.37694483734088 27.161951909476663 27.285007072135787 24.176096181046677 32-33 20.51768033946252 28.533239038189535 26.883309759547387 24.065770862800566 34-35 21.364214992927863 27.525459688826025 27.45827439886846 23.65205091937765 36-37 22.12305516265912 27.0990099009901 26.44483734087694 24.33309759547383 38-39 21.485855728429986 26.917256011315416 27.686704384724187 23.91018387553041 40-41 21.36138613861386 26.525459688826025 27.813295615275813 24.299858557284303 42-43 20.915841584158414 26.174681753889672 28.244695898161243 24.664780763790663 44-45 20.321074964639323 26.38826025459689 28.132248939179632 25.15841584158416 46-47 20.901697312588404 26.24328147100424 28.384016973125885 24.47100424328147 48-49 20.02050919377652 26.775106082036775 28.74823196605375 24.45615275813296 50-51 20.172560113154173 26.695190947666198 28.05162659123055 25.080622347949085 52-53 19.164073550212162 26.91089108910891 28.3995756718529 25.525459688826025 54-55 19.071428571428573 27.48868458274399 28.079207920792083 25.360678925035362 56-57 19.643564356435643 26.553041018387553 27.91654879773692 25.886845827439885 58-59 19.5990099009901 26.45756718528996 27.618104667609618 26.325318246110324 60-61 19.923620933521924 26.395332390381892 27.45049504950495 26.230551626591232 62-63 19.51202263083451 26.549504950495052 27.84370579915134 26.094766619519095 64-65 20.68741159830269 26.651343705799153 26.710042432814713 25.951202263083452 66-67 20.345827439886847 27.04031117397454 26.212164073550216 26.4016973125884 68-69 19.83097595473833 26.940594059405942 26.3988684582744 26.82956152758133 70-71 20.605374823196605 27.338048090523337 25.864214992927863 26.192362093352195 72-73 20.258132956152757 27.018387553041016 26.251768033946256 26.47171145685997 74-75 20.292079207920793 27.475247524752476 26.26096181046676 25.97171145685997 76 20.792079207920793 27.042432814710043 26.274398868458277 25.89108910891089 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 2.5 15 2.5 16 4.0 17 6.0 18 8.0 19 13.0 20 13.5 21 11.0 22 25.0 23 51.0 24 73.5 25 84.0 26 137.5 27 227.5 28 305.0 29 346.0 30 408.0 31 581.5 32 839.0 33 985.0 34 1168.5 35 1512.0 36 1924.0 37 2176.0 38 2408.5 39 3050.0 40 3768.0 41 4233.0 42 4389.0 43 4601.0 44 4980.0 45 5205.5 46 5264.0 47 5427.0 48 5253.5 49 4572.0 50 4227.0 51 3942.5 52 3353.0 53 2804.5 54 2561.0 55 2392.5 56 1941.5 57 1448.0 58 1237.0 59 1051.5 60 782.5 61 617.5 62 536.0 63 441.5 64 319.5 65 240.5 66 157.5 67 126.0 68 116.5 69 87.0 70 58.0 71 49.0 72 41.0 73 21.5 74 12.0 75 14.0 76 13.0 77 9.0 78 8.5 79 11.0 80 7.5 81 4.5 82 5.5 83 6.0 84 4.0 85 1.5 86 1.5 87 2.0 88 1.5 89 2.5 90 2.5 91 2.0 92 3.0 93 1.5 94 0.0 95 0.5 96 1.0 97 1.5 98 2.5 99 3.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.743988684582744 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70700.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.479490806223474 #Duplication Level Percentage of deduplicated Percentage of total 1 74.78074648174588 41.48797736916549 2 9.784825616969202 10.857142857142858 3 5.41760146848868 9.016973125884018 4 3.0160106057515805 6.693069306930694 5 2.0625127472975726 5.721357850070722 6 1.2849275953497858 4.2772277227722775 7 0.8668162349581888 3.3663366336633667 8 0.5787273098103203 2.5685997171145685 9 0.46400163165408936 2.3168316831683167 >10 1.7438303079747093 13.694483734087695 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005657708628005658 0.0 2 0.0 0.0 0.0 0.005657708628005658 0.0 3 0.0 0.0 0.0 0.007072135785007072 0.0 4 0.0 0.0 0.0 0.026874115983026876 0.0 5 0.0 0.0 0.0 0.0297029702970297 0.0 6 0.0 0.0 0.0 0.0297029702970297 0.0 7 0.0 0.0 0.0 0.03253182461103253 0.0 8 0.0 0.0 0.0 0.042432814710042434 0.0 9 0.0 0.0 0.0 0.09618104667609619 0.0 10 0.0 0.0 0.0 0.11598302687411598 0.0 11 0.0 0.0 0.0 0.13154172560113153 0.0 12 0.0 0.0 0.0 0.14285714285714285 0.0 13 0.0 0.0 0.0 0.1471004243281471 0.0 14 0.0 0.0 0.0 0.15983026874115983 0.0 15 0.0 0.0 0.0 0.1669024045261669 0.0 16 0.0 0.0 0.0 0.17114568599717114 0.0 17 0.0 0.0 0.0 0.17963224893917965 0.0 18 0.0 0.0 0.0 0.19943422913719944 0.0 19 0.0 0.0 0.0 0.21357850070721357 0.0 20 0.0 0.0 0.0 0.23196605374823195 0.0 21 0.0 0.0 0.0 0.2376237623762376 0.0 22 0.0 0.0 0.0 0.24893917963224893 0.0 23 0.0 0.0 0.0 0.27015558698727016 0.0 24 0.0 0.0 0.0 0.28005657708628007 0.0 25 0.0 0.0 0.0 0.2857142857142857 0.0 26 0.0 0.0 0.0 0.2956152758132956 0.0 27 0.0 0.0 0.0 0.31541725601131543 0.0 28 0.0 0.0 0.0 0.3408769448373409 0.0 29 0.0 0.0 0.0 0.37482319660537483 0.0 30 0.0 0.0 0.0 0.4016973125884017 0.0 31 0.0 0.0 0.0 0.4497878359264498 0.0 32 0.0 0.0 0.0 0.48514851485148514 0.0 33 0.0 0.0 0.0 0.5261669024045261 0.0 34 0.0 0.0 0.0 0.6039603960396039 0.0 35 0.0 0.0 0.0 0.6973125884016973 0.0 36 0.0 0.0 0.0 0.85997171145686 0.0 37 0.0 0.0 0.0 1.14992927864215 0.0 38 0.0 0.0 0.0 1.5912305516265912 0.0 39 0.0 0.0 0.0 2.046676096181047 0.0 40 0.0 0.0 0.0 2.649222065063649 0.0 41 0.0 0.0 0.0 3.217821782178218 0.0 42 0.0 0.0 0.0 3.8642149929278644 0.0 43 0.0 0.0 0.0 4.471004243281471 0.0 44 0.0 0.0 0.0 5.123055162659123 0.0 45 0.0 0.0 0.0 5.809052333804809 0.0 46 0.0 0.0 0.0 6.38048090523338 0.0 47 0.0 0.0 0.0 7.063649222065064 0.0 48 0.0 0.0 0.0 7.678925035360679 0.0 49 0.0 0.0 0.0 8.319660537482319 0.0 50 0.0 0.0 0.0 8.885431400282885 0.0 51 0.0 0.0 0.0 9.46958981612447 0.0 52 0.0 0.0 0.0 10.067892503536068 0.0 53 0.0 0.0 0.0 10.652050919377652 0.0 54 0.0 0.0 0.0 11.178217821782178 0.0 55 0.0 0.0 0.0 11.783592644978784 0.0 56 0.0 0.0 0.0 12.374823196605375 0.0 57 0.0 0.0 0.0 12.90947666195191 0.0 58 0.0 0.0 0.0 13.441301272984441 0.0 59 0.0 0.0 0.0 13.975954738330977 0.0 60 0.0 0.0 0.0 14.415841584158416 0.0 61 0.0 0.0 0.0 14.910891089108912 0.0 62 0.0 0.0 0.0 15.38048090523338 0.0 63 0.0 0.0 0.0 15.823196605374823 0.0 64 0.0 0.0 0.0 16.343705799151344 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCACC 25 2.3775667E-4 55.990944 42 TGTCAGT 20 0.0069296453 52.491512 19 ATCTATA 20 0.0069296453 52.491512 23 GCGACAA 20 0.0069296453 52.491512 16 TGCTTCG 20 0.0069296453 52.491512 52 ACGCACT 20 0.0069296453 52.491512 8 TAGGTTG 20 0.0069296453 52.491512 32 GCTGCTT 20 0.0069296453 52.491512 50 TTCCGAC 20 0.0069296453 52.491512 24 TGACGCA 20 0.0069296453 52.491512 6 CCATGGA 20 0.0069296453 52.491512 30 GCTTCGC 20 0.0069296453 52.491512 53 TTAGTCC 20 0.0069296453 52.491512 3 TAGATGA 20 0.0069296453 52.491512 10 GACGCAC 20 0.0069296453 52.491512 7 GAACTCT 30 5.8464945E-4 46.659122 22 TTGTCAC 30 5.8464945E-4 46.659122 41 CCTGAAC 30 5.8464945E-4 46.659122 19 ACATATG 40 0.0022752455 35.394848 1 GTCACCA 40 0.0024060677 34.994343 43 >>END_MODULE